Diuresis research work

8/14/2019 Diuresis research work

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Taking urine and fecalprobes from lab animals:

problems and solutions.Stklyanina L.V., Doe Amenyo

Human Anatomy Department,

Lugansk Medical University.(Scientific supervisor- ass.prof.

Stklyanina L.V.).


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Challenging common clinical tasks, such as

monitoring serological conditions of an organism,

the most obvious and initial step in this research is

the analysis of the daily diuresis and urine

contents.

Getting the probe of urine from the lab rats may be

problematic. First, animals during 24-hours monitoring usualy

kept in the isolated chamber without water supply,

that hardly influences on the urine viscosity and

distorts it's chemical properties.

Second, without proper mechanical filtration the

urine probes are usually mixed with the fecals,

spreading by the animal over the floor of chamber.


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Third, it's difficult to collect ALL the

amount of urine without special V-shape

collector.

Fourth, there is no proper construction for

getting both urine and fecals separately at

the same 24h period.

Fifth, individual chambers for lab animals

are too expensive for wide-users' demands.


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So, we are intrigued by the creation of

the simple construction that allows toget the urine and fecal probes and solve

all those problems, mentioned above.


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Our proposition is to use the 10-liters plastic bottle

with the lattices double floor, putting upside down.

Perforatings over the walls of bottle maintains the

proper ventilation.


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Floor arranged as 2 layers. Upper layer settels the

animal and made up with the dural lattice (cell

diameter no more than 3 mm). Important that the

dural metal resists the corrosion and does not interactwith urinary acids.


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Forming the second layer, we used a tea-strainer,

which is firmly wrapped at the container's mouth

around the neck. Diameter of the cells in our tea-

strainer is no more than 1 mm, .so it hampers thesmallest side bodies and clears the urine's efficiently.


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To create a V-shape collector, we took the plastic funnel

and fixed the container inside the wide part of it. Note that

the end of funnel hermetically united with the lab sterile

flask. It is the place where urine accumulates and furthertransport into the chemical laboratory for analysis.


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We also provided proper water supply for animal to

prevent it from dehydration. Hence, at the outer and

inner surfaces on the wall of bottle we fixed the halvesof syringe (5ml), which are interconnected by the

plastic tube.


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Such easy construction works ascommunicative vessels, or ducting.Outer halve, when filled by water,

sends the water slowly into the innerhalve. So, animal receives water adlibitum and can not overflow or spillthe fluid from the drinking through

because of slow release from outervessel.

Outer and inner halves were left withmeasurements, so you may check the

initial and final (after 24h) amount ofthe water in it.

When compared with the amount of thereceived urine (in ml per 24h), it is

the way to calculate 24h-diuresis.


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As a result, our construction houses the rat in rather

comfortable personal reservoir, totally isolated from

external influences and with proper ventilation and

natural drinking regiment.


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So, benefits of our construction are:

1. Our drinking-through releases the water fromouter vessel into the inner one gradually andproportionaly to the water consumption by rat, sonot even drop of water wouldn't be lost. So, anydrug solution may be applied and the calculationof the liquor intake per hour or 24h is very easyand precise.

2. Metal floor works as the primary filter wherethe fecals hampers and accumulates and does not

pollute the urine, excreted freely by animal. Sincethat, it is easy to pick up the fecals for the furtheranalysis.


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3. Urine, passing through the cells of the metal floor(primary filter), flows to the neck of the container underthe force of gravity. Reaching the neck, urine meets with

the secondary filter of the tea-strainer, where it undergoesthe careful refinement second time. Note that the clearedurine enters directly to the sterile flask, which serves as acollector and transport container of the particular probe.So, there is no need to pour the urine into another glass

for the analysis. Such hermetization of the probe excludesthe any interaction of the urine with the externalenvironment and saves the yield of probe lossless.

4. Finally, this device is attractive from an economicstandpoint since it may be properly designed by thecheapest components within 2-3 hours. Daily capacityisn't restricted. Our construction is mobile and light inweight, therefore was created for both animal's and user 'scomfort.


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HOPE, OUR DEVICEHOPE, OUR DEVICE

WOULD BE USEFUL TOWOULD BE USEFUL TO

YOU, MY DEARYOU, MY DEARCOLLEGES.COLLEGES.

THANKS A LOT FORTHANKS A LOT FOR

YOUR ATTENTION!YOUR ATTENTION!

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Diuresis research work