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RESUME

1) PERSONAL DETAILS

FULL NAME Dr.Sandhya Ilanthodi

DATE OF BIRTH 13 October 1977

LICENSURE (KMC) 61512

PERMANENT ADDRESS

“PANCHAVATI”, 1-17-898, Giri nagar Main Road, Landlinks township, Derebail, Konchady, Mangalore. Ph.No: 9611873871Email Address: drsandhyai@yahoo.co.in

2) QUALIFICATION

COURSE UNIVERSITY

MD (PATHOLOGY) A.J. INSTITUTE OF MEDICAL SCIENCES,RGUHSD C P (Diploma clinical pathology) KASTURBA MEDICAL COLLEGE,

MAHE UNIVERSITYM.B, B.S BANGALORE MEDICAL COLLEGE,

BANGALORE UNIVERSITYH.S.C CANARA P.U. COLLEGE,

MANGALOREMETRICULATION B.E.M HIGH SCHOOL, KASARAGOD.

3) DETAILS

UNDERGRADUATE STUDIES: COURSE MBBS

COLLEGE BANGALORE MEDICAL COLLEGE,BANGALORE, BANGALORE UNIVERSITY

DATE OF PASSING: JUNE 2000

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POSTGRADUATE STUDY

COURSE DIPLOMA CLINICAL PATHOLOGY (DCP)

COLLEGE KASTURBA MEDICAL COLLEGE, MANGALORE MAHE UNIVERSITY

DATE OF PASSING DECEMBER 2006

COURSE MD (PATHOLOGY)

COLLEGE A.J.INSTITUTE OF MEDICAL SCIENCES, RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES.

DATE OF PASSING MAY 2011

4) EXPERIENCE

TEACHING EXPERIENCE Two years of undergraduate teaching as tutor during the DCP course at kasturba medical college, Mangalore.Four years of undergraduate teaching experience as senior resident from August 2007 to May 2011, during which period MD (pathology) was also completed at A.J.Institute of Medical Sciences.One year undergraduate and post graduate teaching experience as assistant professor since June 2011 till date. Total teaching experience in years: 7 years

WORKING EXPERIENCE

Worked as a tutor in AJIMS in the department of community medicine and pharmacology from December 2002 to May 2004. Worked as pathologist reporting hematology, cytology and histopathology cases since August 2007 at AJIMS, Mangalore.Presently working as assistant professor and section head for pathology in the central diagnostic laboratory of AJIMS, at AJIMS, Mangalore since June 2011 till date.

OBJECTIVE:  Keen interest in establishing as a pathologist. Well versed with cytology(including FNAC),haematology and histopathology.  

FIELD OF INTEREST:  Cytology, heamatology and histopathaology.

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PUBLICATIONS:

1) Prasad K, Tiwari A, Ilanthodi S, Prabhu G, Pai M. Automation of immunohistochemical evaluation in breast cancer using image analysis. World J Clin Oncol 2011; 2(4): 187-194 Available from: URL: http://www.wjgnet.com/2218-4333/full/v2/i4/187.htm DOI: http://dx.doi.org/10.5306/wjco.v2.i4.187

2) Aravind Pallipady, Sandhya Ilanthodi, Rashmi Va idya, Zulfikar Ahmed, Rithin Suvarna,

Gauri Metkar. A Clinico-Morphological Spectrum of the Non Neoplastic Lesions of the Uterine Cervix at AJ Hospital, Mangalore Journal of Clinical and Diagnostic Research. 2011 June, Vol-5(3): 546-550.

3) Aravind Pallipady, Sandhya Ilanthodi. Pleomorphic lipoma : A cytologicaldiagnostic dilemma. Journal of Clinical and Diagnostic Research. 2011 Apr, Vol-5(2):355-356.

4) Sandhya Ilanthodi, Aravind pallipady, Jayaprakash K, Francis N P Monteiro. Secondary Cardiac Pleomorphic Rhabdomyosarcoma – A Case Report and Review of Literature. Journal of Clinical and Diagnostic Research. 2011 Apr, Vol-5(2):364-366.

CMEs and Conferences attended:

More than twenty CMEs and two conferences attended with poster publication in one state conference. Attended two CMEs as chair person to two case presentations.

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CURICULLUM VITAE

NAME : MUHASIN.TP

DATE OF BIRTH : 18/12/1990

SEX : MALE

AGE : 22 YEARS.

NATIONALITY : INDIAN

ADDRESS : MUHASIN.TP

S/O MUHAMMED.TP

ROSHNA (H). PATTANATHUPARAMBU

POST-KOLATHARA.

CALICUT – 673655

KERALA

CONACT NO :09743502316 (Karnataka)

:09809582204 (Kerala)

QUALIFICATION:

BSC.MLT :A.J.INSTITUTE OF MEDICAL SCINCES

MANGALORE- KARNATAKA (2008-12)

PUC (VHSE-MLT) :G.V.H.S.S. CHERUVANNUR–CALICUT (2006-08)

SSLC :G.V.H.S.S. CHERUVANNUR –CALICUT .(2006

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SYNOPSIS

Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore

“STUDY OF RED BLOOD CELL HISTOGRAM IN

VARIOUS ANEMIAS”.

Name of the candidate : MUHASIN.T.P

Research supervisor (Guide) : Dr. SANDHYA.I

Course and Subject : MSC MLT (HAEMATOLOGY

AND BLOOD TRANSFUSION)

Department of Pathology,

A. J Institute of Medical Sciences,

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Kuntikana, Mangalore – 575004.

2012

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA, BANGALORE.

ANNEXURE II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR

DISSERTATION

1 Name of the candidate and

address (in block letters)

MUHASIN.TP

DEPARTMENT OF PATHOLOGY

A J INSTITUTE OF MEDICAL SCIENCES

MANGALORE-575004

2 Name of the Institution A J INSTITUTE OF MEDICAL SCIENCES

MANGALORE-575004

3 Course of study and Subject MSC MLT (HAEMATOLOGY AND

BLOOD TRANSFUSION)

4 Date of admission to course 27/09/12

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5 TITLE OF THE TOPIC: “STUDY OF RED BLOOD CELL HISTOGRAM IN

VARIOUS ANEMIAS”

6

BRIEF RESUME OF THE INTENDED WORK

6.1 NEED FOR STUDY

The RBC histogram is an integral part of automated hematology analysis

and is now routinely available on all automated cell counters. This histogram

and other associated complete blood count (CBC) parameters have been

found abnormal in various hematological conditions and may provide major

clues in the diagnosis and management of significant red cell disorders.

One histogram graph is worth 1000 numbers. A large collection of data,

displayed as a visual image, can convey information with far more impact

than the numbers alone. In hematology, these data take on several forms, one

of which is the RBC histogram.

Therefore a study of variation in RBC histograms in various anemias is

intend.

6.2 REVIEW OF LITERATURE

Visual scanning of the histogram gives a good initial sense of the range, size,

shape, and other salient features of the red cell morphology.(1)

The various automated red cell parameters involved in anemia work up are

known and studied by various scholars. Anemia classification based on

morphology along with MCV and MCHC is in use presently. Red cell

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deformation and viscosity affects on the MCV and MCHC measurements.

The MCHC from pulse –editing systems should be used only as quality

control parameters. The relevance of a clinically used MCHC and accurate

MCV in the classification of anemia’s is also documented.(2)

Hence there is a need for hematologists to give more clinically useful

opinions on blood samples run on automated analyzers instead of signing out

a parameters littered automated report. Graphical representation of results in

the form of histograms or scatter plots has been largely ignored in favor of

newer numerical parameters that have become available over the years. Some

of these like the red cell distribution width , hemoglobin distribution width,

and reticulocyte hemoglobin content provide very useful information in

addition to the red cell indices that have been traditionally used.(3)

There have been very few studies outlining the utility of red cell histograms

or scatter plots in identification of hematological conditions (4)

Patients with iron deficiency anemia , sequential histograms can show the

progressive appearance of a new well hemoglobinized erythrocyte population

well ahead of that indicated by the conventional numerical parameters.(5)

A histogram can provide useful information for laboratories in 1)Monitoring

the reliability of the results generated by the analyzer 2)investigating the

potential cause(s) of the erroneous automated results3)arriving at the

presumptive diagnosis Eg; certain conditions like the presence of

fragmented red cell or red cell agglutination that could not have been

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identified earlier without blood film examination can now be presumably

detected on the red cell histogram(6)

A fair knowledge of histogram pattern can help to differentiate subclinical

anemias. A study concluded that iron deficiency anemia is characterized by

RBC with decreased hemoglobin concentration and can be distinguished

from beta thalessemia trait which in comparison shows increase in

microcytosis and preserved RBC hemoglobin concentration.(7)

There is dramatic reduction in the number of medical technologists and

technicians in medical laboratories which has been largely taken care of

automation ,especially in hematology. However automation has also created

problems relating to maintenance of expertise especially in the field of

microscope examination(8)

Several attempts have been made to create an expert system with sufficient

accuracy to diagnose classes of anemia directly from the automated

hematology form(9)

The RBC histogram is frequently used along with peripheral smear

examination as an aid in monitoring and interpreting abnormal

morphological changes, particularly Dimorphic red cell population which

should be correlated with numerical data for better interpretation of results as

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it is associated with numerous clinical conditions.(10)

Automated histograms have also been proven to identify red cell fragments

more effectively than routine peripheral smear examinations .RBC fragments

are commonly seen in malignancies with cytotoxic chemotherapy and severe

iron deficiency(11)

6.3 OBJECTIVE OF THE STUDY

1. The objective of the study is to know the utility and advantage of red cell

histograms

2. To study the histogram in various anemias

7.MATERIALS AND METHODS

7.1 SOURCE OF DATA

All pa All anemic patients from Central Diagnostic Laboratory of A.J.IMS.

7.2 METHOD OF COLLECTION OF DATA.

A total of about 100 patients will be included in the study.

7.2 a: TYPE OF STUDY: prospective study.

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7.2 b: SAMPLE SIZE: 100

Sample 7.2c:DURATION OF STUDY: 6 MONTHS

DATA ANALYSIS

The RBC histograms of all anemic patients visiting the Central Diagnostic

laboratory of AJIMS will be analyzed. The RBC histogram will be correlated

with clinical findings when the Hemoglobin is low. The spectrum of

variations in RBC histogram will be tabulated with respect to age groups and

clinical diagnosis. Cases will be cross checked with peripheral smear,

wherever possible.

INCLUSION CRITERIA

All anemic patients with low hemoglobin.

EXCLUSION CRITERIA

Patients with normal hemoglobin.

7.3 DOES THE STUDY REQUIRE ANY INVESTIGATION OR

INTERVENTIONS TO BE CONDUCTED ON PATIENTS OR OTHER HUMANS

OR ANIMALS? IF SO,DESCRIBE BRIEFLY : NO

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7.4 Has Ethical committee clearance? → YES

List of references

1Benie T constantino. CML health care INC, Missisauga, Ontario,Canada “The red cell histogram and the dimorphic red cell population doi:10.1309 /LMF1UY85HEKBMIWO(2011) LabMedicine, 42, 300-308.

2.Anaemia diagnosis ,classification and monitoring, using cell—Dyn technology reviwed for the new millennium. L Van Hove, T schisano, L Brace.Laboratory hematology 6.93-108©2000.Carden Jennings publishing co.Ltd.L

3. Markovic M , Majkic – singh , subota- Reticulocyte hemoglobin content in

the diagnosis of iron deficiency anemia. Clin Lab. 2004., 50: 431-436

Mediline.

4. Radtke H, Meyer T, kallus U: Raoid identification of iron deficiency in

blood donors with red cell indexes provided by Advia 120.

Transfusion .2005: 45:5-10 Mediline.

5. Fossat C , David M, Harlae JR: New parameters in erythrocyte counting

value of histograms . Arch pathol Lab Med. 1987., 111:1150-1154 Medline

6.Gulati GL,Hyun BH: The automated CBC.A current perspective. Hematol

8.

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Oncolcin North Am.1994;8;593-603.Mediline

7.Siemel DL,DelongER, Feussner J, ,Weinberg, JB,Crawford J.Erythrocyte

anisocytosis. Visual inspection of blood films Vs automated analysis of ered

cell distribution with Arch intern Med.1983 Apri;148(4):822-4

8.Pierre RV.peripheral blood film review.The demise of the eye –count

leucocyte differential.ClinicalLabmed march 2002 22(1):279-297

9.Birndorf NI, Pentecost JO,CoakleyJR,Spackman KA.An expert system to

diagnose anemia and report results directly on hematology forms:comput

Biomed Res 1996 Feb;29(1):16-26

10.http://labmed.ascpjournals.org/content/42/5/300.full.

11.d onofrioG, ZinG, RicercuBM, MaciniS,mango G,Automated

measurement of red blood cell microcytosis and hypochromia in Iron

deficiency and thalassemia trait.Arch pathol Lab Med.1992 Jan;116(1):84-9

9 Signature of candidate

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10 Remarks of the guide Study will throw light on RBC histogram variation.

11 Name & Designation of

(in block letters)

11.1 Guide

11.2 Signature

Dr. SANDHYA.I

MBBS,MD,DCP

ASSISTANT PROFESSOR OF PATHOLOGY

AJ INSTITUTE OF MEDICAL SCIENCES,

KUNTIKANA, MANGALORE

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11.5 Head of Department

11.6 Signature

DR. UMARU. N

PROFESSOR AND HEAD OF THE DEPARTMENT,

M.B.B.S, MD,

A J INSTITUTE OF MEDICAL SCIENCES,

KUNTIKANA, MANGALORE- 575004.

12 12.1 Remarks of the

Chairman and Principal

12.2 Signature

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PROFORMA

PATIENT DETAILS:

Name:

Age:

Sex:

Hospital No:

Relevant clinical conditions :

P.smear findings :

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LABORATORY INVESTIGATION

Hb::

RBC indices

M.C.V:

M.C.H:

M.C.H.C:

H.C.T:

R.D.W:

RBC Histogram:

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INFORMED CONSENT FORM

TITLE OF RESEARCH :

“STUDY OF RED BLOOD CELL HISTOGRAM IN VARIOUS ANEMIAS

IN A.J HOSPITAL, MANGLORE’’

INVESTIGATORS: DR.SANDHYA.I

Mr. MUHASIN. T.P

INTRODUCTION:-

You are being invited to participate in research project designed to Study about RBC

histogram in various anemias patients in A.J hospital. You have been selected for this study

because you meet the inclusion criteria of the study.

EXPLANATION OF PROCEDURES:-

In this project, if you are selected, detailed history taking and clinical examination

along with laboratory investigations concerned with the study will also be done.

POSSIBLE BENEFITS:

The investigators do not promise or guarantee that you will receive direct benefit from being in the study. There also may be benefits involved that are not known to the researchers at this time.

POSSIBLE RISKS:-

There are no known physical risks for the child or person associated with these

methods.

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ALTERNATIVE PROCEDURES:-

If you don’t want to participate in the study you will receive the usual care given in

the hospital.

CONFIDENTIALITY:-

The result of the study may be published for scientific purpose, however your

identification will not be revealed.

WITHDRAWAL:-

Participation in the study is voluntary; if you do not wish to participate in the study

you will not lose benefits to which you are entitled. You are free to withdraw your consent

and discontinue your participation in this project at any time.

SIGNIFICANT NEW FINDINGS

Any significant new findings discovered during the course of the study, which may

influence your decisions to continue participations, will be made known to you.

COSTS OF PARTICIPATIONS

The cost of the study will be covered by the investigators or by a research grant,

there will be no additional cost to you for participating in this study.

PAYMENT FOR PARTICIPATION.

There will be no payment to you for participating in this study

Payment of Research Related Injuries:

If, as a result of your participation, you experience injuries from known or

unknown risk of the research procedures as described, immediate care and treatment,

including hospitalization if necessary, will be available. Neither investigator nor A.J.

Institute of Medical Science has made provision for monetary compensation in the event of

injury resulting from the research and in the event of such injury, treatment is provided, at

free of charges

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QUESTIONS:

If you have any questions about this study, questions about a research related injury

or experience, any problems during the study, you should contact Muhasin.Tp. at

9743502316 and Dr Sandhya.I. at 9611873871 . If you have questions about your rights as

a research participant, you may contact Research Ethics Committee, AJIMS, Mangalore.

LEGAL RIGHTS

By signing this consent form, you are not waiving any of your legal rights.

CONSENT STATEMENT

My signature below indicates that I have decided to participate in the study and

that I have read (or been read) the information provided above and that I was given the

opportunity to ask questions and that they have answered to my satisfaction and that I

have received a copy of this signed consent form

__________________________________ _____________________________

Signature of Person Obtaining consent Date

If other than the Principal Investigator

__________________________________ _____________________________

Signature of Principal Investigator Date