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To understand stem cells, one needs to understand the fertilization process

Fertilization process

Stem cells are unspecialized cells that have the potential to become any cell type in the body with an added advantage of dividing and renewing themselves for long periods

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Stem cells are broadly divided into 2 types:- Hematopoietic stem cells- Mesenchymal stem cells

What are stem cells?

• Hematopoietic stem cells are multi-potent stem cells that can differentiate into various blood cell types – RBCs, WBCs, Platelets etc.

• Mesenchymal stem cells - are multi-potent stem cells that can differentiate into osteoblasts and osteocytes (bone cells), chondrocytes (cartilage cells), adipocytes (fat cells), myocytes (heart muscle cells) etc.

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Types of stem cells

Hematopoietic stem cells:

- Umbilical Cord Blood- Bone Marrow- Peripheral Blood

Mesenchymal stem cells:

- Umbilical Cord- Bone Marrow- Adipose Tissue- Dental Pulp

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Sources of stem cells

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Any disease resulting in cellular & tissue destruction can potentially be treated by stem cells as they can be differentiated into various healthy functioning adult cells

Mesenchymal stem cells Bone deformities Arthritis Cardiac Infarct (Heart Attack) Muscular Dystrophy Multiple Sclerosis Immuno modulator in Bone

marrow transplantation Parkinson’s Disease Spinal Cord Injuries Stroke Alzheimer's Disease Diabetes Liver cirrhosis

Hematopoietic stem cells Leukemia (Blood Cancer) Aplastic Anemia Sickle Cell Anemia Thalassemia Hodgkin’s Disease Hunter’s Syndrome

Why are stem cells important?

The list is fast growing…..

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Stem cell banking is a service dedicated to preserving your stem cells for decades to facilitate wide-

ranging therapies in future

What is stem cell banking?

• Umbilical cord blood - The residual blood in the cord and the placenta is a rich source of Hematopoietic stem cells

• Umbilical cord - The umbilical cord contains an inner layer called Wharton’s jelly which is a rich source of Mesenchymal stem cells

• An unique option provided only by Cryo Stemcell – preservation of platelet rich plasma (PRP) from the umbilical cord blood

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What are the banking options?

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Umbilical cord is a conduit between the fetus and placenta, which is physiologically and genetically part of the fetus

Umbilical cord contains two arteries and one vein consisting of cord blood, which is a rich source of Hematopoietic stem cells

Collection of cord blood is painless and safe for you and your baby, and it does not interfere with the birthing process

After the childbirth, the umbilical cord is clamped on both the sides, then cut from the child’s end and the blood is drawn using a syringe into a collection device containing anti-coagulant agents to prevent clotting of the blood

The collected blood is shipped to our laboratory for processing

Umbilical cord blood

Clamping & cutting the cord Extracting the cord blood Off to the bank!

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The blood vessels in the umbilical cord are buried within Wharton's jelly, a gelatinous substance that is a rich source of Mesenchymal stem cells

There are no nerves in the cord, so cutting it isn’t painful for you or the baby

Soon after the birth, post the collection of umbilical cord blood, the delivering doctor will cut the cord between the two clamps and place it into a sterile solution

The collected cord is shipped to our laboratory for processing

Umbilical cord

Cutting the cord Collected cord in solution Off to the bank!

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Unique offering – PRP banking

Platelet Rich Plasma (PRP) contains high concentration of human plateletgrowth factors in a small volume of plasma The concentrated platelets found in PRP contain huge reservoirs of

bioactive proteins, including growth factors that are vital to initiate andaccelerate tissue repair and regeneration These bioactive proteins initiate connective tissue healing: bone, tendon

and ligament regeneration and repair, promote development of new bloodvessels, and stimulate the wound healing process

PRP is isolated and concentrated from umbilicalcord blood post isolation of stem cells from it andis cryopreseved for future therapeutic application

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PRP banking contd..

PRP therapy is non-surgical, with quicker healing times and has shownpromising results for many conditions such as plantar fasciitis, tendonosis,muscle, ligament and tendon strains, sprains and tears (Rotator Cuff,Patella, ACL etc), that have failed other conservative treatment PRP is also widely used in cosmetic applications and hair transplants

Characteristics PRP from Umbilical Cord Blood PRP from Peripheral Blood

Level of growth factors High Low

Mitogenicity High Relatively Low

Proliferation rate of stem cells More Less

Therapeutic potential High Low

PRP from Umbilical Cord Blood vs. PRP from Peripheral Blood

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Who can use these products?

Type of Stem Cell HLA Typing Who Can Use Them?

Cord Blood Stem Cells Required

4 out of 6 HLA Matched Recipients- 100% Match for the Baby (6 out of 6)- 75% Match for Siblings (4 out of 6)- 50% Match for Parents (3 out of 6)

Cord Stem Cells Not Required Anyone in the family

PRP Not Required Anyone in the family

Dhrupad Rao - India’s first child to have preserved his stem cells from cord blood

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About us

India’s first exclusively autologous stem cell preservation company (Estd. 2003)

India’s first and only stem cell bank to offer Platelet Rich Plasma banking

2003 Established India’s first autologous stem cell preservation company

2003 First umbilical cord blood sample processed & preserved successfully

2005 Launched umbilical cord derived Mesenchymal stem cell preservation

2007 Launched expansion & differentiation services

2009 Launched bone marrow derived stem cell preservation

2013 Pioneered PRP preservation from umbilical cord blood

2010 Introduced cord blood plasma separation from umbilical cord blood

2014 Introduced eye-lenticule preservation

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Milestones

• VisionTo enable affordable healthcare by exploiting technology tooffer the best therapeutic options to patients

• MissionTo be a leading stem cell bank and support our clients’therapeutic needs with high quality stem cells

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Vision and Mission

Cryo Stemcell has supported over 110 stem cell transplants across India

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Stem cell therapy

• Expansion & differentiation of stem cells

• Bone marrow processing for stem cell preservation and therapeutic applications

• Peripheral blood mobilized stem cell preservation

• Dendritic stem cell vaccine for cancer treatment

• Preservation of eye lenticules

• Adipose tissue processing for stem cell preservation and therapeutic applications

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Other services rendered

Yearly Payment Option21 Years – One Time

Payment OptionPlan Particulars First Year’s

FeeYearly Fee from

Second Year Onwards

Premium UCB & PRP 40,000 3,000 70,000

Classic UCB & UC 52,000 3,000 82,000

Platinum UCB, UC & PRP 70,000 3,500 1,10,000

Note:

UC: Umbilical Cord derived stem cells

UCB: Umbilical Cord Blood derived stem cells

PRP: Platelet Rich Plasma derived from UCB

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Stem cell preservation plans

Multiple retrievals– Umbilical cord blood stem cells in 4 cryo vials as opposed to 1 cryo bag

Robust methodology enables processing of low cord blood volume– Low cord blood volume is influenced by type of pregnancy, hormonal levels etc.

Unique option of preserving PRP

Post banking services - Expansion and Differentiation services– Expansion of umbilical cord blood stem cells

High cell count & cell viability

Exclusive tie-ups with hospitals for stem cell therapy

Backed by more than 11 years of experience & continuous innovation

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Why Cryo Stemcell?

+91-76769 97616

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Appendix

Human Leukocyte Antigen

6 Loci in their genes: A, B, C, DP, DQ and DR

For baby’s own use, no need of matching HLA, because it’s the same source

Paternal genes and maternal genes come to baby

Chances of HLA matching between blood relatives is very high

If stem cells are not stored, chances of HLA matching from an allogenic bank are 1 in 25,000

HLA Typing

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Father Mother

HLA on Stem Cells

Disease Occurrence

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