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B. C. A. Cell viability (% Control). Cell viability (% Control). siRNA Control PC. APC 0 1 10 µg/ml. - PowerPoint PPT Presentation
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siRNA Control PC APC 0 1 10 µg/ml
Supplemental Figure 1. A) PC/APC expression in RASF detected by immunofluorescent staining. Scale bar: 20 µm. Nuclei were counterstained with DAPI (blue). RASF viability after PC siRNA transfection (B) or treated with recombinant APC (C) for 72 hrs detected by MTT assay and confirmed by trypan blue exclusion assay. Data on graphs are expressed as percentages of control and shown as mean ± SD (n=3). There were no statistical differences.