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ETTORE MAJORANA - CENTER FOR SCIENTIFIC CULTURE

INTERNATIONAL SCHOOL OF PURE AND APPLIED BIOSTRUCTURE: Director: C. Nicolini - 2nd course - "Chemical Carcinogenesis" Directors: E. FARBER, C. NICOLINI - 18-30 October 1981, A NATO ADVANCED STUDY INSTITUTE

LIST OF PARTICIPANTS

Gianni BRAMBILLA

Fredic BURNS

Peter CERUTTI

Giovanni DELLA PORTA

Emanuel FARBER

Charles KING

Istituto di Farmacologia Viale Benedetto XV, 2 16132 Genova, Italy

Institute of Environmental Medicine New York University Hedical Center 550 First Avenue New York, N.Y. 10016, USA

Dept. Carcinogenesis Swiss Inst. for Explto Cancer Res. Ch. de Boveresses CH-1066 Epalinges sur Lausanne, Switzerland

Istituto Nazionale Tumori Via Venezian, 1 Milano, Italy

Dept. of Pathology School of Medicine University of Toronto 100 College Street Toronto, Canada

Dept. of Chemistry and Carcinogenesis Michigan Cancer Foundation

481

482

Roberto MONTESANO

Claudio NICOLINI

Franz OESCH

Silvio PARODI

Manfred RAJEWSKY

Ditta Kavi SARMA

Ron SCHULTE-HERMANN

Paul TS'O

Bernard WEINSTEIN

110 E. Warren Detroit, MI.

IARC Lyon, France

48201, USA

Istituto di Farmacologia Viale Benedetto XV, 2 16132 Genova, italy

PARTICIPANTS

Vorsteher der Abt. Moledularpharma­kologia Pharmakologisches Inst. der Univer­sitat Obere Zahlbacher Strasse 67 D-6500 Mainz, FRG

Istituto Scientifica Studio e Cura Tumori • Viale Benedetto XV, 10 16132 Genova, Italy

Inst. fur Zellbiologie Univer:sity of Essen Hufelanstrasse 55 D-4300 Essen 1, FRG

Dept. of Pathology Faculty of Medicine University of Toronto 100 College Street Toronto, Canada

Inst. fur Toxikologie und Pharmako­logie der Phillips Universitat Marburg, FRG

Division Biophysics School of Public Health John Hopkins University Baltimore, MD 21205, USA

Dept. of Medicine Columbia University College of Physicians and Surgeons New York, NY 10032, USA

PARTICIPANTS

Gerald WOGAN

Peter ABBOTT

Charles ARUS

Cecilia BALBI

Franco BIGNONE

Claudio Blonda

Stefania BONATTI

Franco M. BUffiNAGURO

Marco CAVANNA

Britta CHRISTENSEN

483

Dept. of Nutrition and Food Sciences Massachuettes Inst. of Technology Room 16-333 Cambridge, HA 02l39, USA

Imperial Cancer Res. Fund Laboratories P.O. Box n. 123 -Lincoln's Inn Fields London WC 2A 3PX, UK

Univ. Autonoma de Barcelona Dept. Bioquimica (Ciencias) Bellaterra - Barcelona, Spain

Istituto Scientifico Studio Tumori Viale Bebedetto XV, 10 Genova, Italy

Istituto Giannina Gaslini Via V Maggio Genova - Quarto, Italy

Istituto di Genetica Universita degli Studi Via Amendola 165 Bari, Italy

Istituto di Mutagenesi e Differen­ziamento CNR - Via Svezia, 10 56100 Pisa, Italy

Istituto di Patologia Generale Piazzette Sant' ndrea delle Dame, 2 80138 Napoli, Italy

Istituto di Farmacologia Viale Benedetto XV, 2 16132 Genova, Italy

The Danish Cancer Society Laboratory of Environmental Carcino­genesis Ndr. Frihavnsgade 70 DK 2100 Copenhagen, Denmark

484

Lorenzo CITTI

Amedeo COLUMBANO

Helene COULOMB

Jacques DE GERLACHE

Alain DELEENER

Silvio DE FLORA

Leila DIAMOND

Eugenia DOGLIOTTI

Lars DRAGS TEN

Cecile DREVON

PARTICIPANTS

Istituto di Mutagenesi e Differenzia­mento CNR Via Svezia, 10 56100 Pisa, Italy

Istituto di Patologia Generale I Universita di Cagliari 09100 Cagliari, Italy

Institut de Recherches Scientifiques sur Ie Cancer Boite Postal n. 8 7 rue Guy Mocquet 94800 Villejuif, France

Unit of Biochemical Toxicology and Cancerology Universite' Catholique de Louvain U.C.L. 73.69 B - 1200 Brussels, Belgium

Universiteit Brussel Faculteit der Wetenschappen Algemene Dierkunde Pleinlaan 2 1050 Bruxelles, Belgium

Istituto di Igiene Via Pastore, 9 16132 Genova, Italy

The Wistar Institute Thirty-Sixth Street at Spruce Philadelphia, PA. 19104, USA

Istituto Superiore di Sanita' Roma, Italy

Arbejdsmilj¢ Instituttet Produktregisteret Blegdamsves 104 C 2100 Copenhagen Denmark

Centre International de Recherche sur Ie Cancer 150 Cours Albert-Thomas 69372 Lyon Cedex 2, France

PARTICIPANTS

Mauro DURANTE

Mine ENGINUN

Joseph FRIEDMAN

Chiara GERI

Walter GIARETTI

Maria Ignacia GOMES

Vahdet GUL

Stephen Thomas HADFIELD

Janet A. HALL

J.B. HANSEN

Istituto diGenetica Via Matteotti 1/A 56100 Pisa, Italy

I.U. Kimya Fakultesi Analitik Kimya Kirsusu Istanbul, Turkey

Institut Suisse de Recherches Experimentales sur Ie Cancer rue des Boveresses CH-1066 Epalinges s.1 Lausanne, Switzerland

Istituto di Genetica Via Matteotti 1/A 56100 Pisa, Italy

Istituto di Fisica Sperirnentale Politecnico di Torino Via Duca degli Abruzzi 24 10129 Torino, Italy

Universidade Nova de Lisboa Dep. Bioquimica Campo de Santana 130 1198 Lisboa Codex, Portugal

T.C. Bursa Universitesi Tip Fakultesi Patologi Ve Adli Tip Kursusu Bursa, Turkey

Imperial Cancer Research Fund Labs. P.O. Box n. 123 Lincoln's Inn Fields London, WC 2A 3PX, UK

485

Christie Hospital & Holt Radium Inst. Manchester Area Health Authority South District Manchester M20 9BX, UL

Kemisk Laboratorium II H.C. 0rsted Institutet Universitetsparken 5 2100 Capenhagen, Denmark

486

Filiz HINCAL

Michael A. HUBERT-HABART

D(')n JENS

Marc LANS

Giovanna Maria LEDDA

S. LIMBOSCH

Walter LUTZ

Ottar MADSLIEN

John Hendrick MEERMAN

Dorcas OKOR

PARTICIPANTS

Faculty of Pharmacy Dept. of Analytical Toxicology Hacettepe University Hacettepe, Ankara, Turkey

Istitut Curie - Laboratoire Curie 11 rue Pierre et Marie Curie 75231 Paris Cedex OS, France

Duphar B.V. P.O. Box 2 1380 AA Weesp, The Netherlands

Unit of Biochemical Toxicology and Cancerology Universite' Catholique de Louvain U.C.L. 73.69 B-1200 Brussels, Belgium

Istituto di Patologia Generale I Universita' di Cagliari 09100 Cagliari, Italy

Universite' Libre de Bruxelles Dept. de Biologie Moleculaire Rue de Chevaux 67 1640 Rhode-St-Genese, Bruxelles, Belgium

Institute fur Toxicologie Universitat Zurich Schorenstrasse 16 CH 8603 Schwerzenbach bei Zurich, Switzerland

Norwegian State Pollution Control P.O. Box 8100 Oslo, Norway

Dept. of Pharmacology State University of Groningen Bleomsingel 1 9713 BZ Groningen, The Netherlands

Univers.ity of Benin Department of Chemistry P.M.B. 1154 Benin City, Nigeria

PARTICIPANTS

Paolo PANI

Eligio PATRONE

Veronique PREAT

Paola PRINCIPE

Srinivasan RAJALAKSHMI

Edgar RIVEDAL

Luigi ROBBIANO

Jose' RUEFF

Andrzey SAWARYN

Pev-Olov SCHULTZ

Cattedra di Patologia Generale I Via Porcell 4 09100 Cagliari, Italy

Centro di Studi Chimico-Fisici Istituto di Chimica Industriale Via A. Pastore, 3 Genova, Italy

Unit of Biochemical Toxicology and Cancerology Universite' Catholique de Louvain U • C. L. 73.69 B-1200 Brussles, Belgium

Istituto Superiore di Sanita' Viale Regina Elena 299 00161 Roma, Italy

University of Toronto Dapt. of Pathology Medical Sciences Building 1 Kings College Circle Toronto, M5S lAB, Canada

Lab. for Environmental and Occupational Cancer

487

NHIK - The Norwegial Radium Hospital Montebello, Oslo 3, Norwey

Istituto di Farmacologia Universita' di Genova Viale Benedetto XV, 2 16132 Genova, Italy

Universidade Nova de Lisboa Facultade de Ciencias Medicas, Departmento de Bioquimica Lisboa, Portugal

Freie Universitat Berlin Institut fur Kristalographie Takustrasse 6 1000 Berlin 33/ West Germany

Foretagshalsan, Mellangatan S-57100 Nassjo Sweden

488

Avishay-Ahraham STARK

Maurizio TANINGHER

Willy TAYLOR

Sven Arne THOREN

Jean Jacques TOULME

Gino TURCHI

Luigi VALDATTA

Franz l-lATJEN

William Peter WATSON

A. Kamil PINARCI

PARTICIPANTS

Tel Aviv University Dept. of Biochemistry Ramat-Aviv, Tel Aviv, Israel

Istituto di Oncologia Universita di Genova Viale Benedetto XV 10 16132 Genova, Italy

Department of Physiology The Medical School the University Newcastle upon Tyne NE1 7RU UK

Institute of Hygiene Faculty of Medicine 21 Blegdamsves, DK-2100 Copenhagen ~ Denmark

Museum National d'Histoire Naturelle Laboratoire de Biophysiq~e, INSERM 6J rue Buffon, 75005 Paris, France

Istituto di Mutagenesi e Differen­ziamento CNR, Via Svezia 10 56100 Pisa, Italy

Via Emilia Pavese 117, Castle S. Giovanni 29015 Piacenza, Italy

Kemisk Laboratorium II K0 Benhavns Universitet H.C. 0rsted Institutet Universitetsparken 5 2100 Copenhagen ~ Denmark

Sittinbourne Research Centre Sittinbourne, Kent ME9 BAG, UK

Environmental Engineering Dept. lfuddle East Technical University Inonu Bulvari Ankara, Turkey

SUBJECT INDEX

Acanthamoeba,451 2-Acetylaminofluorene,29,79,lll,

160,383,430 Actin, 451 Actin antibodies,456 Activation, 296 Acyltransferase,34,14 Adriam¥cin,76 AFB1-N -Gua,52,82 Aflatoxin Bl ,47,66,112,142 Alkaline elution,93,117,124,198 Alkilating agents,109 Alkylnitrosamines,365 Ames test,122,135,204 Amines,144 Anabolic Androgens,279 Analgesic Pyrazolon,212 Anchorage-indipendent,348,427 Aniline, 27 Antineoplastic, 210 Aromatic amine,32 Aromatic Hydrocarbons,1,27l Arylamine, 71, 74 Aspergillus Flavus,47

Bacterial mutagenicity,6,204 Benign tumor,315 Benzene, 2 Benzidine, 26, III Benzo(a) anthracenes,3 Benzo(a)pyrene,6,10,111,142,318 Bloom Syndrome,85 Bp metabolities,323 Burkitt's lynphoma,272

Calcium,430,437 Calorimetry, 401 Carbohydrates, 1 Carcinogenici.ty, 26 , 39 Carcinogenic potential,26,l03 Carcinogenic resonse,114,210 Carcinomas, 322, 329,447 Cell cycle,441,4ll,4l3 Centrioles,440 Centrosomes,440 Cigarette, 27 Chemical controls,67-70 Chemical viral interactions,272 Chloroform, 113 Chromatin,81,261,381-432 Chromatin fiber,392 Chromatography,59,180 Chromatosomes,81 Chromosomal aberration,5,86 Chromosomal damage,271 Cimetidine,214 Clastogenicity,87 Cloning efficency,349 Clornaphazin,26 Computer image analysis,384 Congeners, 2 Core-DNA, 79 Covalen t • 77 Cycling Gl> 410 Cyproterone acetate.248 Cytokeratin.454 Cytoskeleton,435-55 Cytoplasmic Microtubule

Complex, 436

489

490

Deacylase,39 Decadurobulin,285 Dehydrogenase,13 DGA,98 Dielectric constant,388 Diethylnitrosamine,279-283,370 Differential inhibition,305 Differential stimulation,305 Differentiation, 355 Dimethylnitrosamine,160 Distamycin A,182 DMNA,383-432,414,430 DMSO,58 DNA

adducts,48 alkaline elution,93-117 binding, 48, 327 conformation,78,179 damage,103-114,306 damagin potency,107 flexibility,96,158 fragmentation,121-147 lesion, 75-92 methylation,179-192 repair,299 sequence, 80 superpacking,417 synthesis, 236 transfection,365

DNase 1,354,428 Dose-response,128 Drugs,193-218

Elasticity,155-163 Elastoniscometer,157 Electric dichroism,180 Electrical property,426 Electron microscopy,383,437 Embryonic fibroblasts,355 Enthalpy, 400 Enzymes,12 Epoxidation,47~66

Epoxide Hydrolase,II,68 Estrogens, 234 Ethylnitrosourea,363 Excretion,61

Feulgen,412 Fibronectin,460 Filter pore,96 Fluorescence,387,413 Foci,167,246 Facs II,387 Freeze etching,397 Functional load,242

Go cells,242,365,407 Gene expression,261

SUBJECT INDEX

Genetic studies,36,351 Genotoxicity,29,193-218,210 Glutamyltransferase,168 Glucuronide, 49 Groove,183 Growth control,252

Heat capacity,399 Hematopoietic tissue,358 Hepatocytes,167 Herbicides,306 Higher order chromatin structure,

84,383 Histamines,214 Histones,81 Hydrazine derivative,1l0,134,198 Hydrogen bonding,189 Hydrolase, 6 Hydrophohic,6 Hydroxyaflatoxin,62 Hormones,315 Hydroxyl-radicals, 75 Hyperplasia, 282 Hypertrophy, 231 Hypolidemic agents,306

ICR-antitumor,207 Induction, 305 Immunofluorescence, 442 Initiation, 167 Initiating agents,262 Initiators,296,334

SUBJECT INDEX

Initiated cells,231,304 Ions,388 Integrated optical density,412 Intracellular water,388

Keratin, 447

Leukemia, 94 Linker, 82 Links, 97 Liposomes,354 Liposomes stimulation,407 Liver architecture,279

Malignant phenotypes,315,364 Melting enthalpy,402 Membrane effects,268 Metabolism, 30, 296 Metabolism of epoxides,4 Metabolites,1,47 Metabolic activation,31 Methyltesterone,382-3 Methylated purines,170 Methylation, 169 Methyl carbonium,183 Methyltransferase,370 Microcalorimetry,385 Micrifilaments,427 Microfilaments pattern,454-6 Microsomes,16,205 Microtubules,427,436-444 Microviscometry,384 Monoclonal antibodies,367 MNNG,186 Model,

elution, 94-103 initiation, 175

Minichromos'omes,83 Mononucleof ilamen t ,390, Monooxygenas-e ,10 Morphological Transformation,

350 mRNA-cDNA lihrary, 357

491

MTOCs,440 Multistage carcinogenesis,295-314

261-275 Multistage theory,318 Myosin, 457

2-Naphthylamine,26 N-aromatic compounds,25-42 Near-ultraviolet light,76,187 Neoplastic transformation,347-361 N-hydroxy-2-acetyl-aminofluorene,

38-41 Nitroreductases,38 Nitrosatable drugs,211 Nitrosamines,297-302 Nitrosocompounds,130,140 N-nitroso,109 N-methyl-N-nitroso-N-nitroguanidine,

179 N-methyl-N-nitrosourea,181 N-7-methyl-guanine,170 N-oxidized derivatives,39 Nuclear-cell morphometry,387 Nuclear pores,408 Nuclear matrix,428 Nucleosomes,81,390

6 °6-ETDGuo,367 °6-methylguanine,170,300 o -12-tetradecanocyl-13-phorbol-

6 acetate,315 o -positive guanine,301 Organ specificity,105 Organotropism, 53 Oxygen, 271

Papillomas, 315-344 Partial-hepatectomy, 417 Pathways DNA,35 Pericentrioles,440 Phenacetine,26 Phase transitions,428 Phenanthrene,17 Phenelzine, 200

492

Phenobarbital, 231-260 Phorboid receptors,266 Phorbol-12-myristate-13-acetate,

86 Phospholipid metabolism,268 Phosphotyrosine,459 Physical agents,80 pp_60src ,456 Preneoplastic nodules,424 Procarcinogens,296 Progression ,259 Progesterone, 248 Promotion, 264-268,315-328 Protein kinase,458 Promoters,76,231-260 Pulse carcinogenesis,373-375

Quaternary structure,389 Quinternary structure,405

Radicals,n Radioimrnunoassay,367 Radiolysis,n Random walk,96 Ranitidine,214 Rat nervous system,363 Repair mechanisms, 299 _ Resting cells,409-413 Revertants, 2,18 Rouss Sarcoma Virus,272,457

Salmonella typhimuriurn,9,29,205 Saturation density,349 Sedatives, 234 Selection regirnens,172,306 Selection procedures,167-176 Short-term tests,121-150 Skin,

carcinoma, 319 papilloma, 315

Smoke, 27 Species specificity, 54-57

SUBJECT INDEX

Soft-agar positive colonies,351 Somatic mutation,350 Stern cells,365 Steroids,231 Strand breaks,

double, lll, 160 single, 107, 160

Stress fiber,454 Sulfatransferase,39

TD50,108 Tertiary structure,389 Tetranuclefilament,393 Therapeutic drugs,193-2I7 Thermal profiles,423 Theory of initiation and promotion, Tissue susceptibility,29 Toxic effects,1,15 Topomyosin,457 TPA,25,315 Transformed phenotype,427,435 Trasmethylation,301 Tropism, 366 Transition probabilities,317 Turnorigenicity,349,366 Tubulin,445

Ultimate carcinogens,79,298 Unscheduled DNA synthesis,37 UV,76,84,187,365

Vimentin,447 Vinculin,459 Viscosity,155,200,403,415

Z-DNA,79

Water,388,414