Caractersticas del Msculo de Pota(Dosidicus gigas)

Caractersticas del Msculo de Pota(Dosidicus gigas)Instituto Tecnolgico Pesquero del Per

Msculo60-80% del peso total y comestible (Sikorski, E. et al 1986)Manto-Aletas-TentculosColorOlorSaborTexturaCaractersticas de sus protenas constituyentesAplicaciones

Color

L*=74.58-72.49/73.97-71.94a* =-2.22 - -2.49b*=2.14-3.16

Donde L= luminosidad, a*= matiz rojo - verde, b*= matiz amarillo -azul

Influencia de la piel y sus pigmentos, manipulacin y el almacenamiento. Reacciones Maillard (azcares-aminocidos)(Moran, 2002; Pariona, 2011)

OlorCaracterstico a Mar en estado fresco, en conservacin vara segn el grado de deterioro alcanzado y los niveles de metabolitos presentes: TMA, BVN-T, Compuestos amoniacales principalmente.Se aprecia mejor en el msculo cocido, mientras ms fresco su olor ser ms intenso (amoniacal).

SaborRelacionado con constituyentes fisiolgicos del msculo y post -mortem:

Snchez - Brambilla, et al. 2004: Compuestos de bajo peso molecular como pptidos y aminocidos solubles en agua.Yamanaka, 1995: Cloruro de AmonioBremner, et al. 1988:Desnaturalizacin de ATP: Hx

TexturaDistribucin de los paquetes musculares fibras radiales y circunferenciales

Fig. 132.Structure of squid (Lolliguncula brevis) (a) cross and longitudinal sections of body; (b) cross-section showing muscle sandwiched between inner and outer tunic; (c) outer tunic consisting of a pattern of collagen molecules forming a cross pattern; (d) muscles forming a pattern that is symmetrical with respect to longitudinal axis (Progress in material science . Autor: Po-Yu Chen, et al. 2012).

Paramiosina y ColgenoProtena del msculo de invertebrados(14%): Elasticidad-elongacin-encogimientoProtena estructural del espacio intercelular Colgeno tipo I.

The Journal of Cell Biology. Epstein, et al. (1985)Torres-Arrola, W et al., (2008)

Protenas constituyentes del Msculo de potaMiofibrilares 75-85% Soluble en soluciones de baja fuerza inica y alta fuerza inicaSarcoplsmicas 10-15% Alta solubilidad en soluciones acuosas de baja fuerza inica Proteasas Baja TransglutaminasaEstroma 12-18%

Variaciones estacionales entre hembras y machosAlta actividad proteoltica

225K150K102K76K52K38K31K24K17K

ABCDEFGA y B: Surimi No reticulado + Extracto B(10 y 15uL respectivamente) C : Extracto A (15uL)F y G: Gel de Surimi (10 y 15uL respectivamente)D y E: Surimi crudo (10 y 15uL respectivamente)MHCMHCPMPMACACTPMTPMMLCMLCMPROTENAS MIOFIBRILARES DE POTA

Utilidad de la protenas musculares segn sus caractersticasMiofibrilar: Carne de la potaGelificacin: Sales de Na y Ca.Emulsificante: Espuma. 5 veces ms que la albmina.

Remocin del Sabor cido-amargo del msculo del manto de potaManto :Composicin proteica y no proteicaEstudios realizadosDesarrollo Tecnolgico de la remocin del saborAplicaciones

Algunos EstudiosFza. gel por sexo y estacionesFza. gel por sexo/estaciones y zonasITP

ITPDela Fuente-Betancourt et al.,2008

Remocin del mal saborCarcter fisiolgico y metablico: NH4 Cl - NH3TamaoOff Flavor

Yamanaka et al., 1994Sanchez-Brambilla et al., 2005Maza et al., 2010

En la IndustriaHasenosa-Alitecno DG 6 plusBudenheimCarnal 659 SAltesaCafodos C

Tratamientos basados en neutralizar el pH- mezcla de fosfatos.Volmenes de agua Tiempo 12-24h de inmersinRegresin del sabor

Remocin del sabor cido-amargo (ITP)

peso (kg)longitud(cm)NTNNPBVNHumedadN-NH4pH0.9537.702.76 0.010.84 0.0181.0 0.879.4 0.077.1 0.06.25 0.02.6049.501.97 0.110.86 0.07294.7 3.887.8 0.04277.4 3.56.45 0.02.8557.002.65 0.020.84 0.0173.8 3.379.4 0.0977.4 0.06.18 0.043.8058.502.70 0.080.95 0.18119.3 8.882.1 0.13154.9 0.36.25 0.05.1061.303.05 0.011.02 0.0547.4 2.179.2 0.1597.4 0.26.38 0.045.6064.002.63 0.030.58 0.04106.2 6.882.3 0.36124.4 6.86.45 0.07.2066.003.17 0.192.04 0.06183.5 7.781.8 0.03182.5 7.66.31 0.018.0069.003.00 0.131.93 0.02183.5 19.582.2 0.08141.1 30.76.28 0.0410.3075.001.74 0.050.82 0.07325.9 3.989.1 0.03306.5 0.26.35 0.014.2081.002.69 0.071.93 0.01313.3 21.885.7 0.56278.6 57.66.28 0.0416.4087.002.67 0.062.02 0.05270.6 3.485.2 0.35293.2 29.96.05 0.026.70100.001.89 0.060.86 0.10340.8 13.388.5 0.53309.7 1.36.2 0.030.40120.002.67 0.050.86 0.17144.0 10.889.0 0.14303.9 15.36.38 0.04

AplicacionesSurimi: imitacin de patas de cangrejo , langostino, langosta, meat balls etcPastas untables: con diferentes saboresAnillas empanizadasSticks/fingersDaruma y piqueosElaboracin de panes y productos de panaderaHojuelas tipo snackHeladosetc etc

16

Aplicaciones

asolari@itp.gob.peDireccin de Investigaciones del ITPGracias!

Grfico2217.5217.5217.5217.587.0396666667108.5733333333122.306666666712674.386666666790.6166666667109.0694.853333333330.136666666724.2466666667101.999666666782.615.4616.1697.446666666783.6939.836666666712.2433333333105.5433333333107.289

BC/FC/Caguacomercialhorasmg N-NH4/100g

tablapeso (kg)longitud(cm)NTNNPBVNHumedadN-NH4pH0.9537.702.76 0.010.84 0.0181.0 0.879.4 0.077.1 0.06.25 0.02.6049.501.97 0.110.86 0.07294.7 3.887.8 0.04277.4 3.56.45 0.02.8557.002.65 0.020.84 0.0173.8 3.379.4 0.0977.4 0.06.18 0.043.8058.502.70 0.080.95 0.18119.3 8.882.1 0.13154.9 0.36.25 0.05.1061.303.05 0.011.02 0.0547.4 2.179.2 0.1597.4 0.26.38 0.045.6064.002.63 0.030.58 0.04106.2 6.882.3 0.36124.4 6.86.45 0.07.2066.003.17 0.192.04 0.06183.5 7.781.8 0.03182.5 7.66.31 0.018.0069.003.00 0.131.93 0.02183.5 19.582.2 0.08141.1 30.76.28 0.0410.3075.001.74 0.050.82 0.07325.9 3.989.1 0.03306.5 0.26.35 0.014.2081.002.69 0.071.93 0.01313.3 21.885.7 0.56278.6 57.66.28 0.0416.4087.002.67 0.062.02 0.05270.6 3.485.2 0.35293.2 29.96.05 0.026.70100.001.89 0.060.86 0.10340.8 13.388.5 0.53309.7 1.36.2 0.030.40120.002.67 0.050.86 0.17144.0 10.889.0 0.14303.9 15.36.38 0.04

Hoja2longitud(cm)peso(kg)NT37.700.952.752.772.760.014043410949.502.601.892.051.970.111533838558.503.802.752.642.700.0795339058longitud(cm)peso(kg)NTNNPBVNHumN NH4pH57.002.852.672.632.650.022674400537.700.952.760.010.840.0081.000.8379.400.0077.100.0064.005.602.612.652.630.027281808149.502.601.970.110.860.07294.670.8087.770.04277.353.466.450.0061.305.103.053.043.050.005023448158.503.802.700.080.950.18119.250.7882.060.13154.860.336.250.0066.007.203.303.033.170.190086552257.002.852.650.020.840.0073.783.2679.400.0977.390.006.180.0469.008.002.913.093.000.128896936264.005.602.630.030.580.04106.186.7682.290.36124.366.826.450.0081.0014.202.742.632.690.073918606761.305.103.050.011.020.0547.382.0479.160.1597.440.216.380.0475.0010.301.771.701.740.047684232266.007.203.170.192.040.06183.530.6981.760.03182.367.596.310.0187.0016.402.632.722.670.061848190669.008.003.000.131.930.02183.4819.4882.170.08141.0730.686.280.04100.0026.701.931.841.890.064630759481.0014.202.690.071.930.01313.3321.8085.690.56278.5757.606.280.04120.0030.402.712.642.670.049176940975.0010.301.740.050.820.07325.613.8589.080.03306.480.216.350.00120.0087.0016.402.670.062.020.05270.613.3685.240.35293.2429.896.050.00longitud(cm)peso(kg)NNP0.000100.0026.701.890.060.860.10340.6313.3488.510.53309.711.286.200.0037.700.950.840.840.840.0022228097120.0030.402.670.050.880.17144.041.7789.000.14303.0015.006.380.0449.502.600.910.810.860.065435786158.503.801.080.820.950.183351097857.002.850.850.840.840.002381745264.005.600.560.610.580.035868875peso(kg)NTNNPBVNHumN NH461.305.100.981.051.020.05221353550.952.760.8481.0079.4077.1066.007.202.081.992.040.06357814022.601.970.86294.6787.77277.3569.008.001.921.951.930.02087668952.852.650.8473.7879.4077.3981.0014.201.931.941.930.00869217713.802.700.95119.2582.06154.8675.0010.300.870.780.820.06919720585.103.051.0247.3879.1697.4487.0016.402.061.982.020.05184432415.602.630.58106.1882.29124.36100.0026.700.930.790.860.09803507867.203.172.04183.5381.76182.36120.0030.400.761.000.880.16949115638.003.001.93183.4882.17141.07120.0010.301.740.82325.6189.08306.48longitud(cm)peso(kg)BVN(mg/100g)0.00014.202.691.93313.3385.69278.5737.700.9581.5880.4181.000.830037032716.402.672.02270.6185.24293.2449.502.60294.10295.23294.670.799030662726.701.890.86340.6388.51309.7158.503.80119.80118.70119.250.777817459330.402.670.88340.0089.00303.0057.002.8576.0871.4773.783.260527851764.005.60101.40110.96106.186.759940828161.305.1045.9448.8247.382.036467529866.007.20183.04184.02183.530.691077407869.008.00169.71197.25183.4819.478456593281.0014.20297.91328.74313.3321.801769905475.0010.30328.33322.88325.613.853731957587.0016.40272.99268.24270.613.3599572107100.0026.70350.06331.20340.6313.3360338932120.0030.40142.79145.29144.041.767766953longitud(cm)peso(kg)HUMEDAD(%)0.00037.700.9579.4079.4179.400.004114809149.502.6087.8087.7587.770.036586205358.503.8081.9682.1582.060.134024708457.002.8579.3479.4779.400.0940216664.005.6082.0382.5482.290.36007530961.305.1079.0579.2679.160.1455768566.007.2081.7481.7881.760.030088707169.008.0082.2382.1182.170.081048199681.0014.2085.2986.0985.690.564409521975.0010.3089.0589.1089.080.033334647987.0016.4085.4984.9985.240.3505168614100.0026.7088.1388.8888.510.5279763077120.0030.4088.9089.1089.000.1414213562longitud(cm)peso(kg)N-NH4(mg/100g)0.00037.700.9577.1077.1077.10049.502.60279.80274.91277.353.460832307358.503.80155.10154.63154.860.327370786757.002.8577.3977.3977.39064.005.60119.54129.18124.366.815774372561.305.1097.5897.2997.440.205972472766.007.20176.99187.72182.367.585107463269.008.00162.76119.38141.0730.675982659281.0014.20319.29237.84278.5757.595533348375.0010.30306.33306.63306.480.214886075787.0016.40314.38272.10293.2429.8940590417100.0026.70310.61308.81309.711.276951808120.0030.40291.90314.40303.1515.9099025767longitud(cm)peso(kg)pH0.00037.700.956.456.456.45049.502.606.256.256.25058.503.806.156.206.180.035355339157.002.856.456.456.45064.005.606.406.356.380.035355339161.305.106.326.306.310.014142135666.007.206.256.306.280.035355339169.008.006.256.306.280.035355339181.0014.206.356.356.35075.0010.306.056.056.05087.0016.406.206.206.200100.0026.706.406.356.380.0353553391120.0030.406.306.256.280.0353553391

datos por tamaosTABULACION DE DATOS PARA EL USO DE ESTADISTICAPOTAPRUEBAS REALIZADASespesor(mm)peso(kg)longitud(cm)NTNNPBVN(mg/100g)HUMEDAD(%)pHNH4(mg/100g)N-NH4(mg/100g)9.150.9537.702.750.8481.5879.406.45522.3077.109.150.9537.702.770.8480.4179.416.45522.3077.1013.212.6049.501.890.91294.1087.806.251069.04279.8013.212.6049.502.050.81295.2387.756.251050.34274.9114.403.8058.502.751.08119.8081.966.19592.58155.1014.403.8058.502.640.82118.7082.156.20590.81154.6314.402.8557.002.670.8576.0879.346.45423.2077.3914.402.8557.002.630.8471.4779.476.45423.2077.3914.935.6064.002.610.56101.4082.036.40456.74119.5414.935.6064.002.650.61110.9682.546.38493.57129.1816.005.1061.303.050.9845.9479.056.32372.8397.5816.005.1061.303.041.0548.8279.266.30371.7297.2919.967.2066.003.302.08183.0481.746.28676.25176.9919.967.2066.003.031.99184.0281.786.30717.23187.7220.388.0069.002.911.92169.7182.236.28621.87162.7620.388.0069.003.091.95197.2582.116.28456.12119.3823.0114.2081.002.741.93297.9185.296.351219.93319.2923.0114.2081.002.631.94328.7486.096.35908.72237.8424.1510.3075.001.770.87328.3389.056.051170.38306.3324.1510.3075.001.700.78322.8889.106.051171.55306.6329.9216.4087.002.632.06272.9985.496.201201.14314.3829.9216.4087.002.721.98268.2484.996.201039.61272.1031.5026.70100.001.930.93350.0688.136.371186.76310.6131.5026.70100.001.840.79331.2088.886.351179.86308.8139.9830.40120.002.710.76380.2089.006.301115.10291.9039.9830.40120.002.641.00320.2088.806.281201.10314.4040.2030.80120.002.801.11330.5081.566.38569.92149.1740.2030.80120.002.830.92310.9081.206.39647.31169.42

por tratamientopHpH inicio6.26.25cf = 1cc =2agua = 3comercial = 4horastratamientopH116.56.500.00BC/FC/Caguacomercial116.516.500.006.320.036.200.007.030.04116.526.500.006.390.016.270.037.080.03126.356.320.0346.540.036.500.016.270.037.100.01126.3186.770.036.670.036.300.007.390.02126.3106.720.036.600.006.310.017.400.00136.26.200.00136.2136.2147.057.030.04horasc/fc/caguacomercial14716.50.006.320.006.200.007.030.041426.500.006.390.016.270.037.080.03216.56.500.0046.540.036.500.016.270.037.100.01216.586.770.036.670.036.300.007.390.02216.5106.720.036.600.006.310.017.400.00226.386.390.01226.4226.4236.256.270.03236.25236.3247.057.080.03247.1247.1416.566.540.03416.55416.5426.56.500.01426.49426.5436.266.270.03436.25436.3447.17.100.01447.09447.1816.756.770.03816.75816.8826.656.670.03826.65826.7836.36.300.00836.3836.3847.47.390.02847.37847.41016.756.720.031016.71016.71026.66.600.001026.61026.61036.326.310.011036.31036.31047.47.400.001047.41047.4

por tratamientopH000.02645751310.0264575131000.03535533910.0353553391000.01154700540.01154700540.02886751350.02886751350.02886751350.02886751350.03214550250.03214550250.00577350270.00577350270.02645751310.02645751310.00577350270.00577350270.02886751350.02886751350.02886751350.0288675135000.01732050810.01732050810.02886751350.0288675135000.01154700540.011547005400

&APage &PBC/FC/CaguacomercialhoraspH

tab-graf-lavadNH4-N inicio220215mg N (NH3)/100gcf = 1cc =2agua = 3comercial = 4horascfccaguacom.192.499107.93124.5115.93184.62108.79121.42131.0718410912113187.03966666674.7380734833108.57333333330.5669509091122.30666666671.91105555471268.7209460496269.4683.88101.14396.87276.793.97113.03793.69277941139474.38666666674.269254423690.61666666675.8341437532109.066.856348080494.85333333331.7533491761426.5228.31100.1187.94431.8922.43102.88979.86432221038030.13666666673.132608072124.24666666673.5255117832101.99966666671.63744017782.64.6251054042815.4818.793.386.027815.514.38100.7482.552815.415.498.382.515.460.052915026216.162.25805225897.44666666673.792694732483.6932.02147050441010.7213.95102.78107.644109.3911.38106.85107.123109.411.4107107.19.83666666670.765005446612.24333333331.4780505179105.54333333332.3942918229107.2890.3076540265horasBC/FC/Caguacomercial0217.53.5355339059217.53.5355339059217.53.5355339059217.53.5355339059187.03966666674.7380734833108.57333333330.5669509091122.30666666671.91105555471268.7209460496274.38666666674.269254423690.61666666675.8341437532109.066.856348080494.85333333331.7533491761430.13666666673.132608072124.24666666673.5255117832101.99966666671.63744017782.64.6251054042815.460.052915026216.162.25805225897.44666666673.792694732483.6932.0214705044109.83666666670.765005446612.24333333331.4780505179105.54333333332.3942918229107.2890.3076540265horasc/fc/caguacomercial187.0 4.7108.6 0.6122.3 1.9126.0 8.7274.4 4.390.6 5.8109.1 6.994.9 1.8430.1 3.124.2 3.5102.0 1.682.6 4.6815.5 0.116.2 2.397.4 3.883.7 2.0109.8 0.812.2 1.5105.5 2.4107.3 0.3

tab-graf-lavad

BC/FC/Caguacomercialhorasmg N-NH4/100g

por tratamiento NH4NH4-N inicio220215217.53.5355339059horastratamientomg N (NH3)/100g1192.499cf = 11184.62cc =21184agua = 312107.93comercial = 412108.791210913124.513121.421312114115.9314131.07141312169.462176.721772283.882293.97229423101.14323113.037231132496.872493.6924944126.524131.8941324228.314222.43422243100.1143102.889.431034487.944479.8644808115.488115.58115.48218.78214.388215.48393.383100.748398.38486.0278482.5528482.510110.721019.391019.410213.9510211.3810211.4103102.78103106.85103107104107.644104107.123104107.1