Chromatography

Sugaralcohols, Mono-and Disaccharides with BioQuant Carbohydrate

Column: PAD ColumnDimension: 250 x 4.6 mm PEEKEluent: 100 mM NaOHFlow: 0.9 ml/minDetection: BioQuant CarbohydrateTemperature: 28 CInjection: 20 lSample: 1 Inositol, 2 Arabitol, 3 Sorbitol, 4 Fucose, 5 Arabinose, 6 Glucose, 7 Xylose, 8 Fructose

9 Lactose, 10 Sucrose (1- 50 mg/l)

no.

171

0 1 2 3 4 5 6 7 8 9 10111213141516171819202122232425262728min

100

200

300

400

500

600

mV

ch1

Inos

itol

Ara

bito

lS

orbi

tol

Fuco

se

Ara

bino

se

Glu

cose

Xyl

ose

Fruc

tose

Lact

ose

Suc

rose

Chromatography

Sugars in Applejuice with BioQuant Carbohydrate

Column: PAD ColumnDimension: 250 x 4.6 mm PEEKEluent: 100 mM NaOHFlow: 0.9 ml/minDetection: BioQuant CarbohydrateTemperature: 28 CInjection: 20 l (diluted 1:1000)Sample: 1 Sorbitol, 2 Glucose, 3 Fructose, 4 Sucrose

no.

172

0 1 2 3 4 5 6 7 8 9 10111213141516171819202122232425262728 min-200

0

200

400

600

mV

ch1

1

2

3

4

Chromatography

Sugars in Applejuice

Column: Hamilton RCX-30Dimension: 150 x 4.6 mm PEEKEluent: 60 mM NaOHFlow: 2,00 ml/minDetection: BioQuant CarbohydrateTemperature: 32 CInjection: 1 l (diluted 1:1000)Sample: 1 Sorbitol, 2 Glucose, 3 Fructose, 4 Sucrose

no.

177

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 min

350

400

450

500

nA

Cell

1

2

3

4

Chromatography

Sugars in Orangejuice (A) and Diet Orangejuice (B)

Column: Hamilton RCX-30Dimension: 150 x 4.6 mm PEEKEluent: 60 mM NaOHFlow: 2,00 ml/minDetection: BioQuant CarbohydrateTemperature: 32 CInjection: 5 l (diluted 1:1000)Sample: 1 Glucose, 2 Fructose, 3 Sucrose

no.

178

1 2 3 4 5 6 7 8 9 10 11min

480 nA

Cell

1

2

3

1 2 3 4 5 6 7 8 9 10 11min

480 nA

Cell

1

2

3

A ) Orangejuice

B ) Diet Orangejuice

Chromatography

Sugars in Shampoo

Column: Hamilton RCX-30Dimension: 150 x 4.6 mm PEEKEluent: 60 mM NaOHFlow: 2,00 ml/minDetection: BioQuant CarbohydrateTemperature: 32 CInjection: 1 l Sample: 3 Sorbitol, 5 Glucose, 6 Fructose, 7 Sucrose

no.

179

0 1 2 3 4 5 6 7 8 9 10 11 min300

400

500

600

700

800

900

1000

nA

Cell

1

2

3

4

5

6

7

Chromatography

Maltooligosaccacharides from Cornsyrup

Column: Hamilton RCX-30Dimension: 150 x 4.6 mm PEEKEluent: Eluent A: 60 mM NaOH

Eluent B: 60 mM NaOH; 500 mM Na-acetatGradient: 10-60% B in 0-20 minFlow: 2,00 ml/minDetection: BioQuant CarbohydrateTemperature: 32 CInjection: 5 l Sample: G4 - G 10

no.

180

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19min

200

400

600

800

nA

Cell

G 4 G

5

G 6 G

7G

8G

9G

10

Chromatography

Monosaccharides of Glycoproteins

Column: Dionex CarboPAC PA1Dimension: 250 x 4.0 mm PEEKEluent: 20 mM NaOHFlow: 1,00 ml/minDetection: BioQuant CarbohydrateTemperature: 28 CInjection: 5 l Sample: 1 Galactosamine, 2 Glucosamine

no.

181

0 1 2 3 4 5 6 7 8 9 10 11 min0

100

200

300

400

500

600

700

800

900

1000

1100

1200

nA

Cell

1

2

Chromatography

Plasma Catecholamines

Column: ProntoSIL-120-3-C18 AQDimension: 33 x 3.0 mmEluent: Pronto-Cat PhaseFlow: 0.5 ml/minDetection: BioQuant CAT Amine, +500 mVTemperature: 30 CInjection: 5 l Sample: 1 Noradrenalin, 2 Adrenalin, 3 DHBA, 4 Dopamin

no.

182

0 1 2 min

2

4

6

8

10

12

14

nA

Cell

1

2

3

4

Chromatography

Catecholamine - 6 - Standard

Column: ProntoSIL-120-3-C18 AQDimension: 33 x 3.0 mmEluent: Pronto-Cat PhaseFlow: 0.5 ml/minDetection: BioQuant CAT Amine, +500 mVTemperature: 30 CInjection: 5 l Sample: 1 Arenalin, 2 DHBA, 3 DOPAC, 4 5-HIAA, 5 HVA, 6 Serotonin

no.

183

0 1 2 3 4 5 min

5

10

nA

Cell

1

23

4

56

Chromatography

Catecholamine - 8 - Standard

Column: ProntoSIL-120-3-C18 AQDimension: 33 x 3.0 mmEluent: Pronto-Cat PhaseFlow: 0.5 ml/minDetection: BioQuant CAT Amine, +500 mVTemperature: 30 CInjection: 5 l Sample: 1 Noradrenalin, 2 Adrenalin, 3 DHBA, 4 DOPAC, 5 Dopamin, 6 5-HIAA, 7 HVA, 8 Serotonin

no.

184

0 1 2 3 4 5 min

5

10

15

20

nA

Cell

1

2 3

4

56

7 8