BO NONG NGH$P v A Pili\ T TRIEN NONG THON qJCQUANLYCIIA.TLtrQ'NG

NONGLAMSANVATIIVYs.AN

sa: 621- /QD-QLCL

C<)NG HOA xA H(>I CHiJ NGHiA ~T NAM Dqc l~p- Tl! do-H~ phuc

Ha N(Ji, ngayJ1tluing./lfliim2013

QVYETDJNII Chi djnh d6i v6i Phong thfr nghi~m chit hrQ11g nong lam thuy san thl}'C phim

TRu m 1A 1 c~K1 LUOn' L1c, v·,, CVCTRUONG CVCQUANLYClIATLUQNGNONGLAMSANvATHUYsAN 1!!Ns· c$3.oJ. A4-can cu QuyBt dinh s6 29/2008/QD-BNN ngay 28/1/2008 CUa B9 tru6ng B9

Nang nghi~p va Phat tri~n nong than quy djnh chuc nang, nhi~m Vl,l, quy€n h~ va ca

cAu t6 chuc clia Cl,Ic Quan ly CMt lugng Nang lam san va Thuy sitn;

Can cu Thong tu s6 16/201 lfIT-BNNPTNT ngay 01/4/2011 cua B9 tru6ng B9

Nang nghi~p va Phat tri~n Nang them quy dµtli v€ danh gici, chi dinh phong thir

nghi~m nganb nong ngbi~p;

Xet Bien ban danh gia lc;i.i va ma r9ng phong ki~m nghi~m chAt lugng nong lam thuy san vting 5 ngay 18/9/2013 cua phong thir nghi~m va Bao cao thgc hi~n kh~c ph\lc 4U c6ng van 264fITCL5-KN ngay 24/ 10/2013 clia Trung tam CMt lugng nong

lam thuy san vilng 5;

Xet d€ nghi clia Truang phong Quan ly KiSm ngbi~m,

QVYET DJNH:

Di~u 1. Chi dinh phong thir ngbi~m cua Trung tam ChAt lugng nong lam thuy

san \Ung 5 (dja chi: 57 Phan Ng9c Hi€~ Kh6m 3, Phuang 6, Tp. Ca Mau di~n thoc;i.i:

0780. 3838396, Fax: 0780. 3830062) la phong thir nghi~m chAt lugng nong lam thuy

san thgc phfun.

Di~u 2. Phong thir nghi~m neu tc;i.i Di€u 1 duqc cAp ma s6 Ia: LAS-NN 84. Quy

each va vi~c sir d\lllg con dAu v€ ma s6 cua Phong thir nghi~m duqc quy dinh 4U Phµ

h,1c 5 Ban hanb kem theo Thong tu s6 16/201 lfIT-BNMPTNT ngay 0114/2011 cua B9

Nong nghi~p va Phat triSn Nang than.

Di~u 3. Phong thir nghi~m neu tc;i.i Di6u 1 duqc tham gia phan tich cac chi tieu

chAt lugng n6ng lam thuy san trong phc;i.m vi neu tc;i.i Ph\11\lc 1 theo yeu cdu cua cac t6

chuc chilng nh~ chAt lugng san phfun n6ng lam thuy san thgc phfun, cac ca quan

quan ly nha nucrc v€ chAt Iugng nong lam thuy san va ctla cac t6 chuc, ca nhan san

xuAt, kinh doanh san phfun nong lam thuy san thgc phfun.

Di~u 4. QuyBt dinh nay c6 hi~u lgc 03 (ba) nam k~ ru ngay Icy.

Di~u 5. Cac Ong Chanh Van phong, Tru6ng phong Quan ly Ki~m nghi~m, Thu

tru6ng cac ca quan, dan vi c6 lien quan va Giam d6c Trung tam Ch~t luqng nong lam

tbuy san vUD.g 5 chju trach nhi~m thi hanh Quy~t djnh nay.

Noinhfi.n: - Nhu Bisu 5;

KT. eve TRUONG _mo eve uONG

- Cl,lC truang (bao cao ); - v\l KHCN (bao cao); - TIT.tin (d~ dang tai website); -Luu VT, KN.

;: .. -- . ,, r

( ~

Phu Jue 1: Danh muc cac chi tieu/ nh6m chi tieu chi djnh cua Phong thfr nghi~m thu9c Truog tam Chit hrqng nong lam thiiy sin vimg 5

(ban hiznh kem theo Quyit iltnh s6 . 1).2r /QD-QLCL ngay J 1 thang !::( niim 2013 - · ---·· - - -·---- ·-cti.a C7!-C Qucin ly efuit-lu(fng Nang lam-san·virThity sanJ---- - -·-- - ---

DBi tirQ'Dg Phuong phap thii' Gi6i h,n phat Ten phep thfr phep thfr h•A ( f. TT 1~n neu

c6)/Ph3m vi do

I. Linh vvc cac phep thfr: Hoa hQc

Thvc phfun AOAC 937.09-1990 -1 xac dinh ham luqng NaCl

Thvc phfun NMKL No 89-1986 -2

Xac dinh ham lugng chat Thµcphfun NMKL No 5.131-1989 -

bee.

3 xac dinh ham luqng

Thµcphfun NMKL No.54-1964 -Formaldehyde

4 Xac dinh ham luqng tro Thµcphfun NMKL

No.23.3rd_ed.1991 -

5 Xac dinh ham luqng fun Thvc phfun NMKL

No.23.3rd.ed.1991 -

6 Xac dinh ham luqng

Thµcphfun NMKL No.6-1976 -Nitrogen.

7 xac dinh ham luqng

Thvc phfun NMKL No.132-1989 lOppm Sulphite

8 Xftc dinh ham luqng TVB-

Thuy san EC/2074/2005 -N

9 Xac dinh ham lm;mg Thuy san

NMKL No 118. 2nd - -Histamine. 1988

IO xac dinh ham 1 uqng

Thuy san TCVN: 3706-1990 -Nitrogen amoniac.

11 xac dinh ham luqng Thvcphfun :NMKL No.57-1994 -

Phospho.

Phat hi~n nhanh 4lP chat Tomnguyen 5.2CL5/ST3.12

12 agar li~u va san

phfun CUa ( NAF 018/10)

0,1%

rom Dinh tinh agar D9ngv~ 5.2CL5/ST3.13

13 thliy san (k~ (NAF 019/10) 0,2% ca tuai va

,.___ _ ph6i tr9n) Phat hl~11 Iilianh 1.?P chdt Tomnguyen 5 .:lCL5/ST3 .14

14 tinh b9t va Polyvinyl li~u va san (NAF 020/ 10) 0,03% alcohol (PV A) phfun ctia

tom Phat hi~n nhanh ~P chat Tomnguyen 5.2CL5/ST3.15

15 CMC li~u va san (NAF 040/10) 0,2%

phfun ctia tom

..

16 Xac dinh ham luqng Thuy san va 5.2CL5/ST4.l (ELISA

0.2ppb Chloramphenicol. m?tong Chloramohenicol T ABP)

17 Xac dinh ham luqng

Thuy san 5.2CL5/ST4.2

0.2ppb Nitrofuran (AOZ). (ELISA AOZ T ABP)

18 xac dinh ham luqng

Thuy san 5.2CL5/ST4.2

0.2ppb Nitrofuran (AMOZ). (ELISA AOZ T ABP)

19 Xac dinh ham luqng nh6m Thuy san

5.2CL5/ST4.3 6ppb

Tetracyclins (ELISA Bioo Scientific) Xac dinh ham luqng

5.2CL5/ST4.4 (ELISA 20 Ciprofloxacin, Thuy san lOppb Enprofloxacin

Quinolones T ABP)

21 xac dinh ham luqng

Thuy san 5.2CL5/ST4.5

lppb trifuraline. (ELISA Bioo Scientific) xac dinh ham luqng 5.2CL5/ST4.6

22 Malachite green, Thliy san (ELISA MG Bioo 1 ppb leucomalachite green Scientific) xac dinh ham luqng 5.2CL5/ST5.1

23 Malachite green, Thuy san (AOAC Vol 78, No.6, 1 ppb leucomalachite green 1995) xac dinh ham luqng nh6m Quinolones: -Oxolinic acid

5.2CL5/ST5.8 -Flumequine 24 -Ciprofloxacine Thuy san

(Journal of food and drug

-Enprofloxacine analysis, Vol IT, 5 ppb

-Sarafloxacine No.2,2003)

-Difloxacine -Danofloxacine

5.2CL5/ST5.3

Xac dinh ham lugng nh6m (USDAFS: Inspection

Tetracyclines Service, Office of Public

25 -Oxytetracycline Thuy san Health and Scence,

-Tetracycline "Qualitative lOppb

-Chlo tetracycline identification of Tetracyclines in

Tissues'') 5.2CL5/ST5.5

Xac dinh ham lugng (Analysis of Spyramicin,

26 Spiramycin trong thuy san bfuJg phuang phap HPLC. Thuy san Tilmicosin and Tylosin

20ppb residue in food by cation exchange solid phase extraction and HPLC)

xac c1inh ham luqng nh6m Sulfonamides -Sulfacetamide -Sulfadiazine -Sulfathiazole

27 -Sulfapyridine Thuy san 5.2CL5/ST5.6 10 ppb

-Sulfamerazine ( AOAC Vol.86, No.3,

-Sulfamethazine 2003)

-Sulfamethoxypyridazine -Sulfachloropyridazine -Sulfaniethoxazole

-Sulfadoxine -Sulfadimethoxine -Sulfachinoxaline

5.2CL5/ST5.9 Xac dinh ham lugng nh6m (Multi-residue analysis Penicilines of penicillin residue in

28 -Amoxcicline Thuysan porcine tissue using 50ppb -Ampicicline matrix solid phase -Benzyl peniciline dispersion

28 TCN197: 2004) 5.2CL5/ST6. l (Analysis of

Chloramphenicol in

xac dinh ham lugng Thuy san va Crawfish meat- Lib

29 4303-FDA O,lppb Chloramphenicol ~tong

Confirmation of multiple phenicol residues in

honey by electrospray LCMS)

5.2CL5/ST6.2 (Detection xac dinh ham lugng nh6m of nitrofuran metabolites Nitrofurans in shrimp - FDA

30 -AOZ

Thuy san A method for the 0,2 ppb

-AMOZ determination of 0,2 ppb -AHD nitrofurans veterinary 1,0 ppb -SEM drugs residues by 1,0 ppb

LC/MS/MS) 5.2CL5/ST6.3

(LIB No. 4396; Volume 23, May 2007.

31 xac dinh ham hrgng SanpMm - Rapid, specific analysis

50ppb Melamine sfra, thuy san of melamine contamination in infant formula and liquid milk

by UPLC/MS/MS).

5.2CL5/ST6.4 Xac dinh ham luqng nh6m ((USDA) : Identification Beta-Agonits: ofBeta-Agonnists by - Clenbuterol HPLC/MS/MS - 0,2 ppb

32 Th.it lqn Revision: 02 Replaces: CLG-AGONl.01

- Salbutamol Effective: 08/27 /2007. 0,5 ppb - Journal of Food and

Dmg Analysis) Xac dinh ham 1 ugng Aflatoxin: -G1 5.2CL5/ST5.10 AOAC

0,6 ppb

33 -B1 Th1Jcp¥iu, (994.08 & AOAC 0,3 ppb

-G2 llgll cOC 998.31)

0,5 ppb

- B2 0,2 ppb

5 .2CL5/ST6. l (Analysis of

Chloramphenicol in

Xac c1inh ham lm;mg Crawfish meat- Lib

34 Thuy san 4303-FDA O,lppb Florfenicol Confirmation of multiple

phenicol residues in honey by electrospray

LCMS)

Xac c1inh ham 5.2CL5/STI. l 35 lm;mgTrifluralin Thuy san ( AOAC 2007.01)

0,5ppb

Xac c1inh ham luc;mg 5.2CL5/ST6.5 Malachite green. Leuco ( AOAC Vol 78, No.6, Malachite green, Crystal 1995 violet, Screening and Leuco Crystal violet. confirmation of

36 Th ' , triphenylmethane dyes 0~5ppb

- - - _uy s~ - - and their matabolites in~ -trout muscle using HPLC

-vis and ESP, LCMS+ Journal of

chromatography B, 788 (2003) 351-359).

Dinh tinh axit boric va 37 mu6i borat. ThvcpMm 28 TCVNl 83: 2003 0,1 %

Xac c1inh, du hrc;mg thu6c trir sau goc Clo -HCB 9,0 ppb

-Lindane 1,2 ppb

- Heptachlor 9,61 ppb \

-Endrin 3 ppb

38 -Aldrin San~Mm, c6 5 .2CL5/ST7 .2 11,5 ppb

- Dieldrin nguon goc ( AOAC 983.21) 12ppb

- Cis chlordane d(mgv~t 3 ppb

- Tran chlordane 2,5 ppb -

-DDT 18,5 ppb

-DDE 35 ppb

- Chlorpyrifos 2ppb

xac c1inh trichlofon - San ~hfun, c6

39 dichlorvos bfuig s§.c Icy khi nguon goc 5.2CL5/STI.3 lOppb

GC/MS d9ng v~t ( AOAC 2007.01)

Xac ctµih Mm lm;mg nh6m Quinolones bfuig phuong phap UPLC/MS/MS: -Oxolinic acid 0.5 ppb

-Flumequine 5.2CL5/ST6.6

0.5 oob (Confirmation of -Enprofloxacine Thuy san va Fluoroquinolones in 0.5 ppb

40 -Sarafloxacine sanphfun Catfish tissue by 0.5 ppb -Difloxacine thuy san Electrospray LC/MS, 0.5 ppb

- Ofloxacine DFS/ORA 0.5 ppb

8/25/97LC/MS/MS)). -Norfloxacine 1.0 ppb

-Ciprofloxacine 1.0 ppb

-Danofloxacine 1.0 ppb 5 .2CL5/ST5 .11

(HPLC Analysis of Six Miscellanous

Xac ctµih Mm lm;mg Thuy san va Antibacterial Drugs clia

41 sanphfun Agilent Technologies 15ppb Trimethoprim thuy san HPLC determination

trimethoprim in meat and milk with SPE

preseparation procedure) 5.2CL5/ST5.12

42 xac dinh ham lm;mg ( HPLC Determination of 30ppb Praziquantel Thuy san Praziquantel in Tablets

and Raw Materials) 5.2CL5/ST7.4

( AOAC 2007.01; AOAC lppb

43 Xac dinh ham luqng

Thuy san Vol.92, No.6, 2009)

Ethoxyquin 05.2 CL5/ST 7.5 (AOAC Vol 92, No.6, lppb

2009, GC/MS)

xac dinh ham luqng kim 5.2CL5/ST8.l

loc;U r$lg bfuig phuang (AOAC

999 .10.2007(1 st.1999) phap ICP/MS - Hg

USDAFSIS, office of 44 -Cd

Th\!C phfun public health science- 5ppb -Pb -Cu CLG-TM3.0l.

- Se Detennination of

-As Cadimium and Lead by

ICPMS (ICP/MS)) xac dinh ham luqng Thuy San Va

05.2 CL5/ST 5.13 45 Nalidixic acid san phfun (HPLC/FLD)

lOppb thuy san

Xac dinh ham luqng Thuy san va 46 Nitroimidazoles ( RNZ, sanphfun 05.2 CL5/ST 6.7 lppb MNZ, MNZ-OH, DMZ, thuy san (LC MS MS)

IPZ, IPZ-OH, DMZ-OH) Xac dinh Mm luqng Nong san

05.2 CL5/ST 6.8 47 Imidacloprid (LC MS MS)

5ppb

48 xa.c dinh ham Iuqng Nang san 05.2 CL5/ST 6.9

5ppb Aldicarb (LC MS MS)

Xac dinh du Iuqng thuoc Thuy san va trir sau g6c clo ( alpha san phfun HCH, beta HCH, gama thuy san. 05.2 CL5/ST 7.6

49 HCH, HCB, Heptachlor, (AOAC 2007.01 lOppb Heptachlor - exo - GC/ECD) epoxide, alpha Endosulfan, beta Endosulfan) xac dinh du luqng thuoc Nong san trir sau g6c clo ( alpha HCH, beta HCH, gama 05 .2 CL5/ST 7. 7

50 HCH, HCB, Heptachlor, (AOAC 2007.01 lOppb Heptachlor - exo - GC/ECD) epoxide, alpha Endosulfan, beta Endosulfan)

II. Linh Vl}'C cac phep thfr: Sinh hoc

f)jnh 1,uc;mg t5ng s6 vi sinh NMKL 86 -4th 2005 > 10 CFU/g

1 Thµc phfun ISO 4833: 2003 > 10 CFU/g v~t hieu. (TPC) TCVN 4884: 2005 ;::: 10 CFU/g

f)jnh luc;mg t5ng s6 Thµc phfun

NMKL 44 - 6th 2004 > 10 CFU/g 2 Coliform ISO 4832: 2007 ;::: 10 CFU/g

Binh lm;mg tong so NMKL 96 - 3ra 2003 >2:MPN/g Coliform, Coliform cb.iu

ISO 4831 : 2006 > 2:MPN/g 3 nhi~t, ,Coliform phan va E. Thµc phfun

coli bang phuang phap ISO 7251: 2005 >2:MPN/g

:MPN

Binh luc;mg tong so

4 Coliform cb.iu nhi~t,

Thi,rc phfun NMKL 125 - 4th

IOCFU/g Coliform phan va E. coli 2005 bfuig phuang phap dB dia

'

f)jnh 1 uc;mg NMKL 66 - 4m 2003 > 10 CFU/g

)

5 Staphylococcus aureus

Thi,rc phfun ISO 6888-1: 2003 2'.: 10 CFU/g bfuig phuang pb.8.p c~y

TCVN 4830-1: trang

2005 ;::: 10 CFU/g

6 f)jnh luc;mg Enterococcus Thi,rc phfun NMKL 68 - 2°d ed. :

> 10 CFU/g 1992

7 Binh Iuc;mg Clostridium

Thµc phfun NMKL 95-3r11 ed.

> 10 CFU/g perfringenes trong 1997

Binh luc;mg Clostridium NMKL 56 -3 ed. :

> 10 CFU/g 8 Th i,rc phfun 1994

khir sulphite ISO 15213: 2003 2'.: 10 CFU/g

NMKL 71 -5 ed.: f)jnh tinh

9 Binh tinh Salmonella spp. Thµc phfun 1999

ISO: 6579: 2007 DµID tinh

TCVN 4829: 2005 Dinh tinh

Dinh tinh Vibrio cholera va Thµc phfun FDA/BAM 2004 Dinh tinh 10 Vibrio parahaemolyticus ISO: 21872-1: 2007 I Dinh tinh Thµc phfun

Thµc phfun ISO 21567: 2004 Dinh tinh 11 Dinh tinh Shigella spp.

Thµc phfun NMKL 151: 1995 Dinh tinh

12 Dinh tinh Listeria monocytogenes

Thµc phfun ISO 11290-1: 2004 Dinh tinh

13 Dinh luqng Listeria Thµc phfun ISO 11290-2: 2004 >IO CFU lg monocytogenes.

Dinh lusmg t6ng s6 nfu:n. NMKL 98 - 41D ed.

> 10 CFU/g 14 Thµc phfun 2005

men, moc trong thµc phfun ISO 21527 -1: 2008 > 10 CFU/g

15 Dinh tinh Thµc phfun ISO 21528-1 : 2004 Dinh tinh Enterobacteriaceae

Dinh luqng Thµc phfun 16 Enterobacteriaceae ky ISO 21528-1: 2004 >2MPN/g

thu~MPN

NMKL 144- 3ro > 10 CFU/g Dinh luqng Thµc phfun 2005

17 Enterobacteriaceae. ISO 21528-2: 2004 2'.: IO CFU/g

18 Dinh tinh E. coli. Thµc phfun ISO 7251: 2005 Dinh tinh

19 Pruit hi~n va dinh luqng

Thµc phfun NMKL 156: 1996 > 10 CFU/g Vibrio spp.

20 Dinh tinh Campylobater

Thµc phfun ISO 10272-1: 2006 E>inh tinh spp.

21 Dinh luqng Campylobacter

Thµc phfun ISO 10272-2: 2006 >IO CFU lg spp ..

Dinh tinh Staphylococcus Thµc phfun ISO 6888-3: 2003 Dinh tinh

22 aureus. TCVN 4830 - 3: 2005 E>inh tinh I

Thµc phfun ISO 7932 3rd ed: 2004 2'.: 10 CFU/g 23 Dinh luqng Bacillus cereus.

Thµc phfun 2'.: 10 CFU/g TCVN 4992: 2005

Dinh luqng Staphylococcus Thµc phfun ISO 6888-3: 2003 >2MPN/g 24 aureus bing phuang pillip

i'v1PN. Thµc phfun TCVN 4830 -3: 2005 >2MPN/g

25 Dinh tinh Coliform Thµc phfun ISO 4831: 2006 Dinh tinh

26 Phat hi~n WSSV Tom vasan 06.2CL5/ST 2.1 va

10 copies phfun tom 06.2CL5/ST 2.2 (PCR)

27 Pruit hi~n YHV /GAV Tom va san 06.2CL5/ST 2.3

20 copies phfun tom (PCR)

28 Phat hi~n IHHNV Tom vasan 6.2CL5/ST 2.5 va

100 copies phfun tom 6.2CL5/ST 2.6 (PCR)

29 PMthi~n TSV I Tom vasan 6.2CL5/ST 2.4 va I phfun tom 6.2CL5/ST 2.6 (PCR) 10 copies

)