SWAMI VIVEKANANDA INSTITUTE OF MODERN SCIENCE Ist year, IInd semester, 2nd Internal Examination, 2011 Paper name: Principles of Microbiology, Paper code: POM-203Time: 3 Hours Full Marks: 70 GROUP – A (Multiple Choice Type Questions)1. Choose the correct alternatives for any ten of the following: 10 ∞ 1 = 10

i) Mitochondria are not found in a) Bacteria b) Eukaryotes c) Algae d) Fungi.ii) Bacteria that live at pH levels from 5 to 8.5 are known as a) Thermophiles b) mesophiles c) Neutrophiles d) capnophilesiii) Which of the following is a neutral stain? a) Picric acid b) Giemsa c) Neutral red d) Malachite green.iv) The colour reaction of a stain is due to a) Chromophore b) Auxochrome c) Mordant d) None of these.v) Some microorganism which utilize radiant energy as their energy source are called a) Phototrophs b) chemotrophs c) Chemoorganotrophs d) chemolithotrophs.vi) Heterocyst in cyanobacteria performs a) Nitrogen fixation b) respiration c) Photosynthesis d) none of these.vii) Which one is an air borne bacterial disease? a) Tuberculosis b) Cholera c) Tetanus d) none of these.viii) The scientist who gave the postulates for "germ theory of diseases" was a) Louis Pasteur b) Robert Koch c) John Needham d) Paul Ehrlichix) Discontinuous heating is called a) Pasteurization b) Sterilization c) Fermentation d) Tindalisationx) Influenza (Flu) is a a) Air borne bacterial disease b) air borne viral disease c) Water borne bacterial disease d) insect borne viral diseasexi) In contrast with moist heat, dry heat probably damages microbes by a) Oxidizing molecules b) denaturing proteins c) Denaturing nucleic acids d) cross-linking DNA.xii) Phenol coefficient indicates a) Efficiency of a disinfectant b) Dilution of a disinfectant c) Purity of a disinfectant d) Quantity of a disinfectant

GROUP – B (Short Answer Type Questions)Answer any three of the following. 3 × 5 = 15

2) Name two bacterial diseases and mention their causative organisms along with parts of the body affected.3) Schematically represent the endospore staining.4) Describe tydallization.5) a) Give the postulates of "Germ Theory of Diseases". b) Define sterilization.6) Describe the different theories of staining.

GROUP – C (Long Answer Type Questions)Answer any three of the following. 3 × 15 = 45

7) Write short notes on any three (3×5=15) a) methanogenic archaea b) halophilic archaea c) thermophilic archaea d) psychrophilic archaea8) Describe Koch's postulate and Koch’s phenomenon. Describe the theory of spontaneous generation with example. Who discovered germ theory of disease and how? What is meant by Biogenesis and Abiogenesis? (5+3+3+4)9) Draw the structure of a typical Bacteriophage. Write a short note on Herpes virus. Give four differences between the bacteria & viruses. (5+5+5)10) Differentiate between sterilization and disinfection. Briefly state the factor responsible for effectivity of antimicrobial agent. Explain with a schematic diagram the operational principle of an autoclave. (3+7+5)11) Explain briefly the three domain classification of microorganism. Classify bacteria based on nutrient requirement. Cite examples. Classify microorganism on the basis of oxygen requirement. (6+5+4)12) Briefly describe the following : dye, chromophores, gram staining, acid fast staining and radiation. (3×5=15)