Excessive sebum formation, follicular epithelial barrier

dysfunction, and inflammatory responses induced by

Cutibacterium acne are major triggering and aggravating

factors for acne. While conventional anti-acne ingredients

addressing sebum formation usually dry up the skin

surface, resulting in skin dryness, Sebodulin restores

healthy hydrolipidic film by activation of autophagy in

sebocytes, the sebum making cells in skin. Sebodulin also

help to alleviate the inflammatory responses by C. acne

and to improve the skin barrier function.

Description

The first sebum formation (sebogenesis) modulating ingredient based on autophagy signaling, helping to restore the healthy hydrolipidic film on skin with anti-inflammatory activity against C. acne

SebodulinRestoration of healthy hydrolipidic film on skin

SebodulinNew Autophagy ACTIVATOR

SebumFormation

AbnormalKeratinization

Inflammation

C. acne Proliferation

Epidermis

Dermis

Fat

ScarFormatiom

Biological activity

New Autophagy Activator

In vitro Anti-inflammatory Activity

Reduction of Sebum Formation

Reduction of Inflammatory Response by C. acne

Sebodulin activates the autophagy in cultured immortalized sebo-cytes(left) and reconstituted skin model(right).

Blank

200

400

1500

300

8000

LC3-ll/LC3-lControl

--

Control

--

T/LA

-+

T/LA

+-

Sebodulin

+-

Sebodulin

++

EGCG

++

EGCG

Trehalose Sebodulin

Auto

phag

y Act

ivity

(%) Po

lar l

ipid

s

IL-8

(pg/

ml)

Neu

tral

lipi

ds

IL-8

(pg/

ml)

150

30076%p

102%p

1000

200

6000

100

200

100

4000

2000

50

100

500

00

0C. acne (1x108CFU/ml)

Sebodulin C. acne (1x107CFU/ml)

Sebodulin

0

0

Trehalose Sebodulin Non-treated

Autophagy (LC3)

Control

C. acne

C. acne + Sebodulin

Reduction of autophagy

Restoration

Inflammation (H&E)

Spongiosis (inflammation)

Anti-inflammation

Sebodulin

Sebodulin inhibits pro-inflammatory cytokine IL-8 secretion in skin kerati-nocytes(left) and immune cells(right) treated with cell free extract of C. acne.

Sebodulin contained product reduces whitehead by 30% than before (by investigator assessment).

Sebodulin reduces lipid synthesis in cultured human sebocytes

Sebodulin preventes reduction of autophagy and increase of inflamma-tion caused by topical application of C. acne in reconstituted skin model

Sebodulin contained product reduces quantity of skin surface lipid (by sebumeter).

Clinical efficacy

PCPC/INCI Name

• Pentasodium Tetracarboxymethyl Heptadecanoyl Dipeptide-12

Composition

• Sebodulin, 1,2-Hexanediol, Water

Application

• Anti-inflammation • Anti-greasiness• Anti-ACNE

Recommended Dosage

• 1~2% for cream, lotion and essence • 0.01~0.1% for masksheet

keratinocytes immune cells

110

100

110

Sebu

m c

ount

(% o

f sta

rt)

Skin

surfa

ce li

pid(

% o

f sta

rt)

90

105

60

90

85

70

9580

100

50 80

****

Vehicle

Before Before

Vehicle

Sebodulin

Week 8Before BeforeWeek 8 Week 8 Week 8

Sebodulin

Whitehead Skin surface lipids