View
996
Download
2
Category
Tags:
Preview:
DESCRIPTION
Types of Entomopathogenic Fungi like Verticillium lecanii Beauveria bassiana Nomuraea rileyi Metarrhizium anisopliae(detailed procedure of mass production in bio control lab)
Citation preview
DEPARTMENT OF
AGRIL.ENTOMOLOGY
K.K.WAGH COLLEGE
OF AGRICULTURE, NASHIK .
Course No : AEL –ENT-486
Credits = (0+20=20)
Course Title : Mass production of
bioagents & bio-pesticides
GENERAL INFORMATION OF THE STUDENT
NAME OF THE STUDENT : Solunke Balram D
REGISTRATION NO : AKN-108/08
MASS PRODUCTION OF Metarhizium anisopliae
(Deuteromycota; Hyphomycetes)
MASS PRODUCTION OF BIOAGENTS
& BIO-PESTICIDES
An entomopathogenic fungus is fungal species that can act as a parasite of insects and kills or seriously disables them. These fungi usually attach to the external body surface of insects in the form of microscopic bodies (usually asexual, mitosporic spores also called conidia).
Entomopathogenic Fungi
Verticillium lecaniiBeauveria bassianaNomuraea rileyiMetarrhizium anisopliae
Types of Entomopathogenic Fungi
Metarrhizium anisopliae is a widely distributed soil-inhabiting fungus. The spore of M. anisopliae can be formulated as dust and sprayable formulation. It is used to control termites, mosquitoes, leafhopper, beetles etc.
Metarhizium anisopliae
Metarhizium anisopliae
Termites & leafhopper :
Mosquitoes & beetles :
rhinoceros beetle Oryctes rhinoceros
The rhinoceros
beetle, Oryctes
rhinoceros is one
of the serious
and important
pests of coconut
and has a wide
distribution and
persistent
occurrence in all
the coconut
growing areas in
the country.
rhinoceros beetle
• The adult beetle cause severe damage to the
growing palms by feeding on the tender fronds and
crowns and resulting in stunting of the trees. The
damage to the spathe results in the loss of nuts.
Young seedlings are sometimes killed outright.
The chemical control measures adopted against this pest are always costly, tedious and have to be repeated
Hence an easy method utilizing the safe and specific fungus, Metarhizium anisopliae for the management of this pest is aimed at.
ISOLATION OFMETARHZIUM &
MEDIA PREPARATION
(1) Cassava
chips mixed with rice
bran supplemented with urea or fish meal
extract
(2)Coconut
water wasted from
copra making
industry
(3)carrot broth.
Production procedure
On coconut water
LAMINAR AIR FLOW
The bottles are inoculated in a laminar flow chamber and rested on flat surface for 2 days or till the surface of medium is fully covered by the olive green sporulated fungus.
The whole culture is crushed thoroughly in an ordinary mixer and used in the field. From a single average size coconut 5-6 bottles of cultures can be made.
The flasks are incubated at room temperature. The spores can be harvested in a fortnight.
From a clean uncontaminated mother culture in slant loopful quantities of M. anisopliae spores are transferred aseptically.
The flasks are allowed to cool and taken to laminar flow chamber for inoculation
The conical flasks are plugged with cotton and autoclaved for 20 min at 15 psi.
Carrot cut into small pieces (40 g) is washed in potable water and transferred to conical flask (250 ml) and 15 ml of distilled water is
added.
In Carrot broth
Spore inoculated flask :
Thank you
Recommended