DEPARTMENT OF

AGRIL.ENTOMOLOGY

K.K.WAGH COLLEGE

OF AGRICULTURE, NASHIK .

Course No : AEL –ENT-486

Credits = (0+20=20)

Course Title : Mass production of

bioagents & bio-pesticides

GENERAL INFORMATION OF THE STUDENT

NAME OF THE STUDENT : Solunke Balram D

REGISTRATION NO : AKN-108/08

MASS PRODUCTION OF  Metarhizium anisopliae

(Deuteromycota; Hyphomycetes)

MASS PRODUCTION OF BIOAGENTS

& BIO-PESTICIDES

An entomopathogenic fungus is fungal species that can act as a parasite of insects and kills or seriously disables them. These fungi usually attach to the external body surface of insects in the form of microscopic bodies (usually asexual, mitosporic spores also called conidia).

Entomopathogenic Fungi

Verticillium lecaniiBeauveria bassianaNomuraea rileyiMetarrhizium anisopliae

Types of Entomopathogenic Fungi

Metarrhizium anisopliae is a widely distributed soil-inhabiting fungus. The spore of M. anisopliae can be formulated as dust and sprayable formulation. It is used to control termites, mosquitoes, leafhopper, beetles etc.

Metarhizium anisopliae

Metarhizium anisopliae

Termites & leafhopper :

Mosquitoes & beetles :

rhinoceros beetle Oryctes rhinoceros

The rhinoceros

beetle, Oryctes

rhinoceros is one

of the serious

and important

pests of coconut

and has a wide

distribution and

persistent

occurrence in all

the coconut

growing areas in

the country.

rhinoceros beetle

• The adult beetle cause severe damage to the

growing palms by feeding on the tender fronds and

crowns and resulting in stunting of the trees. The

damage to the spathe results in the loss of nuts.

Young seedlings are sometimes killed outright.

The chemical control measures adopted against this pest are always costly, tedious and have to be repeated

Hence an easy method utilizing the safe and specific fungus, Metarhizium anisopliae for the management of this pest is aimed at.

ISOLATION OFMETARHZIUM &

MEDIA PREPARATION

(1) Cassava

chips mixed with rice

bran supplemented with urea or fish meal

extract

(2)Coconut

water wasted from

copra making

industry

(3)carrot broth.

Production procedure

On coconut water

LAMINAR AIR FLOW

The bottles are inoculated in a laminar flow chamber and rested on flat surface for 2 days or till the surface of medium is fully covered by the olive green sporulated fungus.

The whole culture is crushed thoroughly in an ordinary mixer and used in the field. From a single average size coconut 5-6 bottles of cultures can be made.

The flasks are incubated at room temperature. The spores can be harvested in a fortnight.

From a clean uncontaminated mother culture in slant loopful quantities of M. anisopliae spores are transferred aseptically.

The flasks are allowed to cool and taken to laminar flow chamber for inoculation

The conical flasks are plugged with cotton and autoclaved for 20 min at 15 psi.

Carrot cut into small pieces (40 g) is washed in potable water and transferred to conical flask (250 ml) and 15 ml of distilled water is

added.

In Carrot broth

Spore inoculated flask :

Thank you