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05/01/2023 1
WELCOME
05/01/2023 2
Dr. Md. Eiktier DewanRoll No. 0213/10Registration No. 174Session: 2013-2014
SUPERVISOR NAME
Prof. Dr. Abdul AhadHead, Dept. of Microbiology and Public Health, CVASU
PRESENTED BY
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THESIS TITLE
Transfer Rate of Maternal Antibody (MaAb) Against Newcastle Disease Virus from Parents to Day Old Chicks.
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Contents
Introduction Objectives Materials and Methodology Results & Discussion Conclusion & RecommendationAcknowledgements
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New Castle Disease (ND) also known as Ranikhet Disease (RD)
Highly contagious viral disease
Attacks many species of domestic and wild birds.
Caused by New Castle Disease Virus (NDV)
Is a –ve sense ssRNA virus
Family Paramyxoviridae.
Introduction
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ND is a major problem of the large and small scale
poultry industries in Bangladesh
Vaccination has been reported as the only
safeguard against endemic ND
Level of MaAb transfer and its half life time is
important for designing a suitable vaccination
program
Cont…
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To estimate the transfer rate of anti-NDV Abs from dams to their offspring in the Breeder Farm
To estimate the transfer rate of anti-NDV Abs from dams to their offspring in the Layer Farm
Detecting the persistence of MDA in progeny from vaccinated parent stock
To provide a guideline in vaccination schedule
Objectives
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Materials & Methodology
Study Area
Study was undertaken within Jan. 2014 to Dec. 2014.
Three renowned Breeder Farms (BF) and 3 Layer Farms (LF) were selected randomly from Chittagong
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10 parent’s serum, 10 eggs & 10 DOCs serum samples from each BF out of total 3 BF
In each breeder farm samples were taken from 3 age group i.e. 34, 38 & 42wks
Similarly 10 parent’s serum, 10 eggs samples were taken from each LF out of total 3 LF
From each LF sample were taken from 3 age group i.e. 34, 38 & 42wks
Cont…
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Cont…
Sampling layout for BF
Age group(wks) Parents serum DOC’S Serum Egg yolk
34 10 10 1038 10 10 1042 10 10 10
Sampling layout for LF
Age group(wks) Parents serum Egg yolk
34 10 10
38 10 10
42 10 10
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Blood and eggs from each BF & each LF were collected randomly by single visit and stored at -200c after serum collection
After 21 days from 1st visit, the DOCs were collected from the hatchery of respective BF
Day old chicks (DOCs) were sacrificed and Serum was then separated and stored at -20oC until analyzed
All the tests were done in the CVASU microbiology and the PRTC laboratory
Cont…
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The assay(ELISA)was performed according to the protocol and suppliance provided by the Biochek commercial ELISA kit.
Cont…
B. Separation of serum from blood A. Collected sera samples
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Data was stored in a Excel sheet.
The immune status of the birds and comparison was done using analysis of variance (ANOVA).
A confidence level of 95% was as a cut off to show the level of significance.
Cont…
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Age in
wks
Parent DOC Egg yolk
Mean ±
SD
P value Mean ±
SD
P value Mean ±
SD
P
value
34 10933.6±
2300.75
.774 9805.9±
4843.35
.096 14081±21
90.43
.336
38 10211.1±
2190.60
5784.4±
3110.09
12537.9±
2965.51
42 10586.2±
2245.35
9055.2±
4535.7
13671±18
07.13
Antibody titre against ND in Breeder Farm (BF1)
Results & Discussion
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Antibody transfer percentage (%) in BF1.
34 wks 38 wks 42 wks0
20
40
60
80
100
120
140128.79 122.79 129.14
89.66
56.65
85.54yolk%DOC%
Cont…
AGE(WKS) Type21 Killed27 Live33 Live39 Live45 Live
Maintained ND vaccination schedule
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Age in wks Parent DOC Egg
Mean ± SD P value Mean ± SD P value Mean ±
SD
P
value
34 15381.6±36
33.67
.006 10814.4±1
919.86
.316 12394±1
591.76
.005
38 18274.2±21
00.72
12299.3±1
828.7
14993.5±
2628.18
42 13240.3±36
68.77
11726.4±2
639.38
10967.5±
1636.34
Antibody titre against ND in Breeder Farm (BF2)
Cont…
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34 wks 38 wks 42 wks0
102030405060708090
100
80.58 82.05 82.8370.3 67.3
88.57
yolk %DOC %
Antibody transfer percentage (%) in BF2.
Cont…
AGE(WKS) Type
20 Killed
27 Live
34 Live
41 Live
48 Live
Maintained ND vaccination schedule
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Age in
wks
Parent DOC Egg
Mean ±
SD
P value Mean ±
SD
P value Mean ±
SD
P
value
34 12971.5±
1957.66
.180 9280±326
2.97
.198 11627.1±
4357.31
.849
38 11345.8±
1352.27
9265.8±1
786.33
9779.4±3
860.25
42 10820.7±
3875.56
8648.7±2
357.07
8714.7±2
257.94
Antibody titre against ND in Breeder Farm (BF3)
Cont…
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Antibody transfer percentage (%) in BF3.
34 wks
38 wks
42 wks
0102030405060708090
10089.64 86.19 80.54
71.5481.67 79.93
yolk%DOC%
Cont…
AGE(WKS) Type
22 Killed
29 Live
36 Live
43 Live
50 Live
Maintained ND vaccination schedule
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Age in wks Parent Egg
Mean ± SD P value Mean ± SD P value
34 10229.3±321
7.5112
.383 6749.2±253
1.4799
.052
38 12434.3±364
1.9266
4473±1418.
6051
42 11885.9±401
8.0102
5296.9±190
1.8988
Antibody titre against ND in Layer Farm (LF1)
Cont…
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Age in wks Parent Egg
Mean ± SD P value Mean ± SD P
valu
e
34 14710.4±1713.
8471
.0004 7094.4±3418.
4783
.148
38 13526.3±1826.
6526
7082.8±1235.
7472
42 10881.3±2188.
8898
5361.6±1144.
6295
Antibody titre against ND in Layer Farm (LF2)
Cont…
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Age in wks Parent Egg
Mean ± SD P value Mean ± SD P
valu
e
34 10395.1±2332.
1158
.717 7949.9±1695.
8026
.017
38 11398.4±1953.
9885
5998.3±2039.
1278
42 10961.1±3643.
2464
5497.6±1883.
2991
Antibody titre against ND in Layer Farm (LF3)
Cont…
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Antibody transfer percentage (%) from parent to egg yolk in Layer Farms.
34 WKS
38 WKS
42 WKS
0102030405060708090
65.98
35.98 44.5648.23
52.36 49.27
76.48
52.62 50.16LF 1LF 2LF 3
Cont…
Age Type
16 18 Killed
24 25 24 live
32 32 31 live
40 39 38 live
48 46 45 live
LF1 LF2 LF317
Maintained ND vaccination schedule
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Cont…ELISA test of our study revealed that, the anti-NDV titre of the mentioned BF and LF were different at different ages. Vaccination protocol in breeder and layer farms mainly
leads this variation.
Infection load of NDV also plays significant role in this aspect.
Thereafter ELISA is a sensitive test not specific leads a little deviation of expected solid & accurate estimation and it can detect all functional types of Abs.
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ELISA is therefore, proved more practical, sensitive and rapid for detection of Ab titres against NDV vaccines compared to the HI test.
The obtained results ,revealed that ELISA can detect high levels of Abs and considered accurate and sensitive compared to HI test.
Cont…
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The estimated half life time of ND maternal antibody level is 6.3 days
In G e o r g i a Po u l t r y, 2010 – 2012 the baseline Broiler Breeder NDV titers at 30-34wks was 3435, at 35-39wks was 3493 & at 40-44wks was
Conclusion & Recommendation
In BF1 transfer rate of MaAb was 89%, 56% & 85% respectively after 1wk, 5wks & 3wks of post vaccination
So we concluded our study findings as following:
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In BF2 transfer rate of MaAb was 70.3%, 67.3% & 88.57% respectively after 1wk, 4wks & 2wks of post vaccination
In BF3 transfer rate of MaAb was 71.54%, 81.67% & 79.93% respectively after 5wks, 2wks & 4wks of post vaccination
DOC from BF1 from 34 & 42wks should be vaccinated within 10 days whereas DOC from 38wks should be vaccinated within 7 days
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DOC from BF2 from 34 & 42wks should be vaccinated within 10 days whereas DOC from 38wks should be vaccinated within 15 days
DOC from BF3 from 34 & 38wks should be vaccinated within 10 days whereas DOC from 42wks should be vaccinated within 7 days
According to this study, the maternally derived antibodies persists up to 5 to 15 days in progeny after hatching & vaccine must be given within this time
Cont…
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I express my gratitude to my supervisor Prof. Dr. Abdul Ahad . I also extend my thanks to all members of the dept. of Microbiology and Veterinary Public Health.
•Dr. Nitish Chandra Debnath , Professor, Department of Microbiology and Veterinary Public Health•Dr. Paritosh Kumar Biswas , Professor, Department of Microbiology and Veterinary Public Health•Dr. Abdul Ahad , Professor, Department of Microbiology and Veterinary Public Health•Dr. Himel Barua , Associate Professor, Department of Microbiology and Veterinary Public Health•Dr. Abul Kashem , Assistant Professor, Department of Microbiology and Veterinary Public Health•Dr. Md. Zohorul Islam, Assistant Professor, Department of Microbiology and Veterinary Public Health•Dr. Mukta Das Gupta, Assistant Professor, Department of Microbiology and Veterinary Public Health
ACKNOWLEDGEMENTS
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Thanks to All
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