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WELCOME

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Dr. Md. Eiktier DewanRoll No. 0213/10Registration No. 174Session: 2013-2014

SUPERVISOR NAME

Prof. Dr. Abdul AhadHead, Dept. of Microbiology and Public Health, CVASU

PRESENTED BY

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THESIS TITLE

Transfer Rate of Maternal Antibody (MaAb) Against Newcastle Disease Virus from Parents to Day Old Chicks.

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Contents

Introduction Objectives Materials and Methodology Results & Discussion Conclusion & RecommendationAcknowledgements

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New Castle Disease (ND) also known as Ranikhet Disease (RD)

Highly contagious viral disease

Attacks many species of domestic and wild birds.

Caused by New Castle Disease Virus (NDV)

Is a –ve sense ssRNA virus

Family Paramyxoviridae.

Introduction

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ND is a major problem of the large and small scale

poultry industries in Bangladesh

Vaccination has been reported as the only

safeguard against endemic ND

Level of MaAb transfer and its half life time is

important for designing a suitable vaccination

program

Cont…

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To estimate the transfer rate of anti-NDV Abs from dams to their offspring in the Breeder Farm

To estimate the transfer rate of anti-NDV Abs from dams to their offspring in the Layer Farm

Detecting the persistence of MDA in progeny from vaccinated parent stock

To provide a guideline in vaccination schedule

Objectives

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Materials & Methodology

Study Area

Study was undertaken within Jan. 2014 to Dec. 2014.

Three renowned Breeder Farms (BF) and 3 Layer Farms (LF) were selected randomly from Chittagong

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10 parent’s serum, 10 eggs & 10 DOCs serum samples from each BF out of total 3 BF

In each breeder farm samples were taken from 3 age group i.e. 34, 38 & 42wks

Similarly 10 parent’s serum, 10 eggs samples were taken from each LF out of total 3 LF

From each LF sample were taken from 3 age group i.e. 34, 38 & 42wks

Cont…

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Cont…

Sampling layout for BF

Age group(wks) Parents serum DOC’S Serum Egg yolk

34 10 10 1038 10 10 1042 10 10 10

Sampling layout for LF

Age group(wks) Parents serum Egg yolk

34 10 10

38 10 10

42 10 10

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Blood and eggs from each BF & each LF were collected randomly by single visit and stored at -200c after serum collection

After 21 days from 1st visit, the DOCs were collected from the hatchery of respective BF

Day old chicks (DOCs) were sacrificed and Serum was then separated and stored at -20oC until analyzed

All the tests were done in the CVASU microbiology and the PRTC laboratory

Cont…

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The assay(ELISA)was performed according to the protocol and suppliance provided by the Biochek commercial ELISA kit.

Cont…

B. Separation of serum from blood A. Collected sera samples

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Data was stored in a Excel sheet.

The immune status of the birds and comparison was done using analysis of variance (ANOVA).

A confidence level of 95% was as a cut off to show the level of significance.

Cont…

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Age in

wks

Parent DOC Egg yolk

Mean ±

SD

P value Mean ±

SD

P value Mean ±

SD

P

value

34 10933.6±

2300.75

.774 9805.9±

4843.35

.096 14081±21

90.43

.336

38 10211.1±

2190.60

5784.4±

3110.09

12537.9±

2965.51

42 10586.2±

2245.35

9055.2±

4535.7

13671±18

07.13

Antibody titre against ND in Breeder Farm (BF1)

Results & Discussion

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Antibody transfer percentage (%) in BF1.

34 wks 38 wks 42 wks0

20

40

60

80

100

120

140128.79 122.79 129.14

89.66

56.65

85.54yolk%DOC%

Cont…

AGE(WKS) Type21 Killed27 Live33 Live39 Live45 Live

Maintained ND vaccination schedule

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Age in wks Parent DOC Egg

Mean ± SD P value Mean ± SD P value Mean ±

SD

P

value

34 15381.6±36

33.67

.006 10814.4±1

919.86

.316 12394±1

591.76

.005

38 18274.2±21

00.72

12299.3±1

828.7

14993.5±

2628.18

42 13240.3±36

68.77

11726.4±2

639.38

10967.5±

1636.34

Antibody titre against ND in Breeder Farm (BF2)

Cont…

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34 wks 38 wks 42 wks0

102030405060708090

100

80.58 82.05 82.8370.3 67.3

88.57

yolk %DOC %

Antibody transfer percentage (%) in BF2.

Cont…

AGE(WKS) Type

20 Killed

27 Live

34 Live

41 Live

48 Live

Maintained ND vaccination schedule

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Age in

wks

Parent DOC Egg

Mean ±

SD

P value Mean ±

SD

P value Mean ±

SD

P

value

34 12971.5±

1957.66

.180 9280±326

2.97

.198 11627.1±

4357.31

.849

38 11345.8±

1352.27

9265.8±1

786.33

9779.4±3

860.25

42 10820.7±

3875.56

8648.7±2

357.07

8714.7±2

257.94

Antibody titre against ND in Breeder Farm (BF3)

Cont…

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Antibody transfer percentage (%) in BF3.

34 wks

38 wks

42 wks

0102030405060708090

10089.64 86.19 80.54

71.5481.67 79.93

yolk%DOC%

Cont…

AGE(WKS) Type

22 Killed

29 Live

36 Live

43 Live

50 Live

Maintained ND vaccination schedule

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Age in wks Parent Egg

Mean ± SD P value Mean ± SD P value

34 10229.3±321

7.5112

.383 6749.2±253

1.4799

.052

38 12434.3±364

1.9266

4473±1418.

6051

42 11885.9±401

8.0102

5296.9±190

1.8988

Antibody titre against ND in Layer Farm (LF1)

Cont…

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Age in wks Parent Egg

Mean ± SD P value Mean ± SD P

valu

e

34 14710.4±1713.

8471

.0004 7094.4±3418.

4783

.148

38 13526.3±1826.

6526

7082.8±1235.

7472

42 10881.3±2188.

8898

5361.6±1144.

6295

Antibody titre against ND in Layer Farm (LF2)

Cont…

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Age in wks Parent Egg

Mean ± SD P value Mean ± SD P

valu

e

34 10395.1±2332.

1158

.717 7949.9±1695.

8026

.017

38 11398.4±1953.

9885

5998.3±2039.

1278

42 10961.1±3643.

2464

5497.6±1883.

2991

Antibody titre against ND in Layer Farm (LF3)

Cont…

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Antibody transfer percentage (%) from parent to egg yolk in Layer Farms.

34 WKS

38 WKS

42 WKS

0102030405060708090

65.98

35.98 44.5648.23

52.36 49.27

76.48

52.62 50.16LF 1LF 2LF 3

Cont…

Age Type

16 18 Killed

24 25 24 live

32 32 31 live

40 39 38 live

48 46 45 live

LF1 LF2 LF317

Maintained ND vaccination schedule

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Cont…ELISA test of our study revealed that, the anti-NDV titre of the mentioned BF and LF were different at different ages. Vaccination protocol in breeder and layer farms mainly

leads this variation.

Infection load of NDV also plays significant role in this aspect.

Thereafter ELISA is a sensitive test not specific leads a little deviation of expected solid & accurate estimation and it can detect all functional types of Abs.

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ELISA is therefore, proved more practical, sensitive and rapid for detection of Ab titres against NDV vaccines compared to the HI test.

The obtained results ,revealed that ELISA can detect high levels of Abs and considered accurate and sensitive compared to HI test.

Cont…

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The estimated half life time of ND maternal antibody level is 6.3 days

In G e o r g i a Po u l t r y, 2010 – 2012 the baseline Broiler Breeder NDV titers at 30-34wks was 3435, at 35-39wks was 3493 & at 40-44wks was

Conclusion & Recommendation

In BF1 transfer rate of MaAb was 89%, 56% & 85% respectively after 1wk, 5wks & 3wks of post vaccination

So we concluded our study findings as following:

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In BF2 transfer rate of MaAb was 70.3%, 67.3% & 88.57% respectively after 1wk, 4wks & 2wks of post vaccination

In BF3 transfer rate of MaAb was 71.54%, 81.67% & 79.93% respectively after 5wks, 2wks & 4wks of post vaccination

DOC from BF1 from 34 & 42wks should be vaccinated within 10 days whereas DOC from 38wks should be vaccinated within 7 days

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DOC from BF2 from 34 & 42wks should be vaccinated within 10 days whereas DOC from 38wks should be vaccinated within 15 days

DOC from BF3 from 34 & 38wks should be vaccinated within 10 days whereas DOC from 42wks should be vaccinated within 7 days

According to this study, the maternally derived antibodies persists up to 5 to 15 days in progeny after hatching & vaccine must be given within this time

Cont…

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I express my gratitude to my supervisor Prof. Dr. Abdul Ahad . I also extend my thanks to all members of the dept. of Microbiology and Veterinary Public Health.

•Dr. Nitish Chandra Debnath , Professor, Department of Microbiology and Veterinary Public Health•Dr. Paritosh Kumar Biswas , Professor, Department of Microbiology and Veterinary Public Health•Dr. Abdul Ahad , Professor, Department of Microbiology and Veterinary Public Health•Dr. Himel Barua , Associate Professor, Department of Microbiology and Veterinary Public Health•Dr. Abul Kashem , Assistant Professor, Department of Microbiology and Veterinary Public Health•Dr. Md. Zohorul Islam, Assistant Professor, Department of Microbiology and Veterinary Public Health•Dr. Mukta Das Gupta, Assistant Professor, Department of Microbiology and Veterinary Public Health

ACKNOWLEDGEMENTS

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Thanks to All