Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest...

Recombinant DNA technologyDNA isolated from

two sources and cut by the same enzyme

Gene of interest

RecombinantDNA

Plasmid(vector)

Transgenic organisms

Useful products

Gene for pestresistance

Gene fortoxic-cleanup

bacteria

Some uses of proteins:

Protein for“stone-washing”

jeans

Protein fordissolving

clots

Some uses of genes:

Restriction Endonucleases

Recombinant DNA technology

First DNA Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”

Recombinant DNA technology

Cloning Vectors: pBR322 plasmid

Recombinant DNA technology

Genetically Modified (GM) FoodsIn the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified.

●Corn has been genetically modified to resist insect

infestation.

Golden Rice

Genetically Modified (GM) Foods

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Recombinant DNA technology

Weeding Out Land Mines

First Glowing Pet

Recombinant DNA technology

Molecular Techniques•Nucleic Acid Extraction

Buffer Components:

SDS (sodium dodecyl sulfate)NaClSodium citrateEDTA

•Polymerase Chain Reaction