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Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms Useful products Gene for pest resistance Gene for toxic-cleanup bacteria Some uses of prot Protein for “stone-washing” jeans Protein for dissolving clots Some uses of genes:

Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

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Page 1: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Recombinant DNA technologyDNA isolated from

two sources and cut by the same enzyme

Gene of interest

RecombinantDNA

Plasmid(vector)

Transgenic organisms

Useful products

Gene for pestresistance

Gene fortoxic-cleanup

bacteria

Some uses of proteins:

Protein for“stone-washing”

jeans

Protein fordissolving

clots

Some uses of genes:

Page 2: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Restriction Endonucleases

Recombinant DNA technology

Page 3: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

First DNA Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”

Recombinant DNA technology

Page 4: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Cloning Vectors: pBR322 plasmid

Recombinant DNA technology

Page 5: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Genetically Modified (GM) FoodsIn the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified.

●Corn has been genetically modified to resist insect

infestation.

Page 6: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Golden Rice

Genetically Modified (GM) Foods

Page 7: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Recombinant DNA technology

Page 8: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Weeding Out Land Mines

First Glowing Pet

Recombinant DNA technology

Page 9: Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms

Molecular Techniques•Nucleic Acid Extraction

Buffer Components:

SDS (sodium dodecyl sulfate)NaClSodium citrateEDTA

•Polymerase Chain Reaction