View
234
Download
0
Tags:
Embed Size (px)
Citation preview
Recombinant DNA technologyDNA isolated from
two sources and cut by the same enzyme
Gene of interest
RecombinantDNA
Plasmid(vector)
Transgenic organisms
Useful products
Gene for pestresistance
Gene fortoxic-cleanup
bacteria
Some uses of proteins:
Protein for“stone-washing”
jeans
Protein fordissolving
clots
Some uses of genes:
Restriction Endonucleases
Recombinant DNA technology
First DNA Cloning Experiment
Cohen et al., 1973
“Recombinant DNA”
Recombinant DNA technology
Cloning Vectors: pBR322 plasmid
Recombinant DNA technology
Genetically Modified (GM) FoodsIn the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified.
●Corn has been genetically modified to resist insect
infestation.
Golden Rice
Genetically Modified (GM) Foods
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615
“Supermice”
Recombinant DNA technology
Weeding Out Land Mines
First Glowing Pet
Recombinant DNA technology
Molecular Techniques•Nucleic Acid Extraction
Buffer Components:
SDS (sodium dodecyl sulfate)NaClSodium citrateEDTA
•Polymerase Chain Reaction