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Biotechnology Rauful Hossain Leutrim Cahni

Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

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Page 1: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

BiotechnologyRauful Hossain Leutrim Cahni

Page 2: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

What is it?

• Biological processes that enable us to quantify molecular biology and can be modified for certain purposes.

It includes• Gel electrophoresis• PCR- Polymerase Chain Reaction• Plasmids• Transformation/Transfection• Recombinant DNA

Page 3: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

Recombinant DNA Gel Electrophoresis

PCR Plasmid

Molecules containing DNAfrom two or more organisms

Restriction enzymes added to DNA containing solutionTo cut certain, specific DNA sequences.

Gel is placed with a positive charge at one end, and a negative Charge on the other. Electricity runs through it to separate

DNA by size

DNA primers are addedTo pre-existing strands

To be able and replicate DNA

Circular piece of DNA containingOne chromosome and is easily modified

Via addition of genes

Page 4: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

Transformation/Transfection

When a recombinant DNA is inserted into a host cell in order to producemore copies of the desired gene.

Its done by injecting the plasmid throughthe membrane after the bacteria have undergoneheat shock

Page 5: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

JEOPARDY

Page 6: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

Gel Electrophoresis

Recombinant DNA

Gene Manipulation

PCR Transformation/Transfection

Applications in the world

To cut up certain, specific DNA sequences in an attempt to separate them for evaluation

Restriction Enzymes Collection of DNA fragments

DNA primers added for replication

Recombinant DNA inserted into host cell

Used as innovations to improve lifestyle

To make it easier to observe the DNA through the gel

Same sequences read from opposite sides

Its size DNA or RNA molecule that acts as starting point for 3 prime chain growth

Electroporation, Chemically treating the cell

It can recognize DNA sequences

To separate the DNA strands based on length and size

Molecules that bond and carry foreign DNA to cells

Selectable marker genes

DNA template Opens up the membrane pores to let in the vector

Vaccines contain a viral strand of DNA that has been made non lethal or weakened- through removing genes.

How long certain gene sequences are and whose DNA matches

DNA ligase cDNA RNA transcriptase Normal vectors don’t have genes supporting the expressing of those genes

Health risks, not natural, and obstruction with destiny.

This is when Biologists or forensic specialists analyze a gel electrophoresis experiment.

Plasmids contain no introns, which cause many disruptions to gene addition. But, eukaryotic DNA contains plenty.

Stem cells, can be transformed with recombinant DNA

denaturation, annealing, elongation

Transgenic Animal The gene coding for insulin wouldn’t have been able to be transfected into our somatic cells

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Page 7: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

QUESTIONS

Page 8: Biotechnology Rauful Hossain Leutrim Cahni. Recombinant DNA Gel Electrophoresis PCR Plasmid

Why use restriction enzymes?

What enzymes are used to cut DNA sequences?

What is produced to cut DNA molecules?

What is a genomic library?

What is a vector? What is biotech generally used for?

Why use a dye on the DNA?

What is a palindrome? What makes E.coli easy to manipulate?

What is a primer? What are methods to insert DNA into host cells?

What can electrophoresis do in criminal investigations?

What is the role of the electrical current running through the gel?

What are vectors? What genes confer resistance to antibodies?

What is targeted by the PCR?

What does the heat shock do to the host cell?

How are vaccines made?

What can seeing how DNA separates lead you to conclude?

Which enzyme is used to bind foreign DNA to a vector plasmid

What can be used to make genes transcribed to a particular tissue?

What is not required in PCR reactions?

Why aren’t normal vectors able to be used in transfection?

What ethical issues are anti-biotech?

What are RFLPs? How are plant cells good hosts for recombinant DNA?

What are the 3 main steps in a PCR cycle?

What is an animal that has gained new genetic information from foriegn DNA

Without biotech, why would diabetes be a bigger problem in the world?