Monoclonal Antibody Against Nsp2 Protein of the Highly Pathogenic Porcine Reproductive and...

Monoclonal Antibody Against Nsp2 Proteinof the Highly Pathogenic Porcine Reproductive

and Respiratory Syndrome Virus

Antigen Used for Immunization

Recombinant Nsp2 produced in Escherichia coli.

Method of Immunization

Six to eight-week-old BALB/c mice were immunized threetimes at 2-week intervals with 100mg of the pET28a-Nsp2protein.

Parental Cell Line Used for Fusion

Splenocytes from the BALB/c mice immunized with Nsp2protein were fused with mouse myeloma cells (SP2/0) ac-cording to conventional hybridoma technology.

Selection and Cloning Procedure

Hybridoma cells were grown in HAT selection medium. Po-sitive clones were subcloned by limiting dilution and screenedby ELISA analysis.

Heavy and Light Chains of Immunoglobulin

The MAb belonged to the IgG1subtype, and the light chainwas k type.

Specificity

The specificity was determined by Western blot and IFA an-alyses. The results clearly showed that this MAb could rec-ognize and bind to the Nsp2 specifically.

Specific Antigen Identified

Non-structural protein Nsp2 of the highly pathogenic porcinereproductive and respiratory syndrome virus.

Availability

Tissue culture supernatant Yes X NoAscitic fluid Yes X NoHybridoma cells Yes No X

Reference

Li B, Du L, Sun B, Yu Z, Wen L, Zhang X, Guo R, Ni Y, Hu Y,Zhou J, Zhu H, Lv L, Yu Y, Wang X, and He K: Monoclonalantibodies against Nsp2 protein of the highly pathogenicporcine reproductive and respiratory syndrome virus.Monoclon Antib Immunodiagn Immunother 2013;32(5):362–365.

Address correspondence to:

Dr. Bin LiInstitute of Veterinary MedicineJiangsu Academy of Agricultural Sciences50 Zhong-ling StreetNanjing 210014Jiangsu ProvinceP.R. ChinaE-mail: libinana@126.com

MONOCLONAL ANTIBODIES IN IMMUNODIAGNOSIS AND IMMUNOTHERAPYVolume 32, Number 5, 2013ª Mary Ann Liebert, Inc.DOI: 10.1089/mab.2013.0048

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