PROJECT Save An Hour - SAH

FRED MARTIN12/02/2009 Dr. GiorgiBIOSC 2

Information on Specimens Used:

General: All slides were prepared slides taken from the MMP slide libraryFluorescent channels on Lycopodium BF and Fluoro images were acquired manually due to NIS exposure changing issue in auto mode. Lily Anther and Brine Shrimp were acquired in auto-mode.All post image processing done in ImageJMetaData Format: Date/Scope/Objective; (Channel): Exposure/ BlackLevel/ Gamma/ WhiteLevel

Specific:

Used For Fluorescent Images

Specimen A1: Invitrogen Slide #4 16 µm cryostat section of mouse intestine w/ DIC: stained with Alexa

Fluor 350 (blue) wheat germ agglutinin to stain the mucus of goblet cells. The filamentous actin prevalent in the

brush border was stained with red-orange–fluorescent Alexa Fluor 568 phalloidin (A-12380) and SYTOX Green nucleic acid stain (S-7020) Specimen B: Lily Anther Cross Section with Pollen (Carolina Slide # nnnn)Specimen C1: Lycopodium Mature Strobillus (longitudinal section) (Slide # nnnn)

Used For Brightfield (Pseudo colorization) Images:

Specimen D: Brine Shrimp (Artemia Nauplii) Whole Section mount (Olympus Sample Slide # nnnn)Specimen E: Lycopodium Mature Strobillus (longitudinal section) (Slide # nnnn)

Used For Extra Credit/Fluorescent/DIC Overlay + DIC only Images:

Specimen F: Typha Latifolia (ls) slide # nnnnSpecimen A2: Invitrogen Slide #4 16 µm cryostat section of mouse intestine w/ DIC: area 2Specimen C2: Lycopodium Mature Strobillus (longitudinal section)

Invt4_10x_Com

positeRG

B_002

.jpg

20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095

SP

EC

IME

N (A

1)

20x_LilyAnther_F

luro_RG

B.jpg

20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830

SP

EC

IME

N (B

)

FM

_lycopodiumC

aptured_F

luoroRG

BnD

IC_20x_2.jpg

20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830

SP

EC

IME

N (C

)

fm_

_pseudocolor_lycopod_20x_Com

positeRG

Bn

DIC

.jpgS

PE

CIM

EN

(D)

20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830

Multichannel _S

hrim

p_Pseudocolor2_R

GB

.jpgS

PE

CIM

EN

(E)

20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged

Extra Credit Image Section:

Specimen F: Typha Latifolia (ls) Fluorescent Composite + 3 channels

Specimen A2: DIC/Fluro Overlay Invitrogen Slide #4 16 µm cryostat section of mouse intestine : area 2

Specimen C2: DIC only: Lycopodium Mature Strobillus (longitudinal section)

Specimen C3: BF Z-Stack: Lycopodium Mature Strobillus (longitudinal section) 11 images in Nikon ND2 file format: attached as separate file named : FredMartinSAH_Zstack.nd2

SP

EC

IME

N (F

)T

ypha_10x_Com

posite_001_RG

B_A

dj

20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095

COMPOSITE CHANNEL: RED

CHANNEL: GREEN CHANNEL: BLUE

invt4_a1_Com

posite_RG

_DapiLU

T_75.jpg

20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830

SP

EC

IME

N (A

2)

SP

EC

IME

N (C

2)F

M_lycopodium

Captu

red DIC

10ms20x.JP

G

20091207 / Nikon2 / 20x ; DIC: 40ms/ 79 / 0.94 / 3,310