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Nikon TE2000U

Inverted Fluorescence MicroscopeDépartement de biochimie, UdeM

Nikon TE2000UCondenser Module 

• To adjust the heigh of the module

• Polarizer

• Centering screws

Aperture diaphragme (plays on contrast and resolution)

Condenser Turret:•A:       brightfield•Ph1:   phase contrast (20x)•Ph2:   phase contrast (40x)•DIC M: Nomarski (40x  with corr. Ring)•DIC H:  Nomarski (60x  and 100x)

• Condenser lens (O.N.: 0.52)

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Objective characteristics• Optical Corrections

• Spherical aberrations (PLAN = corrected)

• Chromatic aberrations (APO = corrected for blue, green and red)

• Magnification (here 10x)

• Numerical aperture (here 0,45)

• Dry or immersion (here dry)

• Method(s) of contrast (here Nomarski)

• Working distance in mm (here 4 mm)

• Coverslip thickness to use (here coverslip No 1 ½ of 0,17 mm thick)

• Lens tube length (infini)

NIKON TE2000U TRANSMITED LIGHT  MICROSCOPY PARTS 

13

14

15

6

5

4

2 ‐ 3

1

9

10

11

8

7

16‐17

18

21

20

7

22

19

12

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NIKONTE2000UINVERTEDFLUORESCENCEMICROSCOPEPARTSUSEDFORTRANSMITEDLIGHTMICROSCOPY

1. Tungsten lamp2. IR:Infrared filter3. NBC:Neutral Color Balancing filter4. Fielddiaphragm5. Condensermodule6. Stage7. Objectives8. XYmovements9. 1,5Xlens10. Coarse andfinefocusknobs (Donottouch,usetheZ‐motor)11. Opticalpath switchover dial(1‐100%Eyepieces,5‐ 100%Camera)12. Eyepieces13. Eyepiece tube turret (O:open;M:2.5xmagnifier;C:close;B:Bertrandlens)14. FrontPort15. Photomask dial16. D:Diffuser17. ND:Neutral density filter18. Analyser19. Tungsten lamp voltagecontrol20. ZMotor21. Camera22. Dia‐illuminationON/OFFswitch

NIKONTE2000UEPIFLUORESCENCEMICROSCOPYPARTS

28

25

2631

28

22

18

29

24

27

23

30

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NIKONTE2000UINVERTEDFLUORESCENCEMICROSCOPE

PARTSUSEDFORFLUORESCENCEMICROSCOPY

18. Analyser to remove from light path when observing in fluorescence

22. Dia‐illumination to turn OFF when observing in fluorescence

23. Mercury lamp

24. Uniblitz motorised fluorescence shutter

25. Neutral density filters (ND4 and ND8)

26. Heat absorbing filter (HA)

27. Manuel fluorescence shutter (O: open; C: close)

28. Fluorescence filter cube turret (6 positions)

29. Fluorescence aperture diaphragm (A)

30. Fluorescence field diaphragm (F)

31. UV screen

Starting sequence fordevices

8

6

4

5

1

2

3

7

Fill uplogbook

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Microscopeaccessories

Écrans

Ordinateur

Z Motor (Prior ProScan)

Uniblitz Shutter

Caméra CoolSnap Fx

Dia lamp control box

Lampe au mercure

Pile de secours

Climatizationdon’t touch