Fernando

Liza Santuza Omar

Alan Júlio

Paola

Lucas

ÍtaloRaphael

Carlos

Marianna

PriscilaClara

Our team

Minas Gerais, Brazil

Where we came from

Where we came from

Belo Horizonte - MG

Where we came from

Universidade Federal de Minas Gerais (UFMG)

Acute Coronary Syndrome (ACS)

NIH Medline PlusWinter 2009 Issue: Volume 4 Number 1

A worldwide problem

Acute Coronary Syndrome (ACS)A worldwide problem

WHO, 2013

Acute Coronary Syndrome (ACS)A Brazilian problem

Teich et al., Rev Bras Cardiol., 2011

Acute Coronary Syndrome (ACS)A challenge

• To develop a sensitive device capable ofdetecting and integrating the signals of ACSbiomarkers, setting a prognosis.

Aiming high

Biomarkers

Tang et al., N Engl J Med., 2013

Trimethylamine-N-OxideTMAO: Introduction

‘

Trimethylamine-N-OxideTMAO: Construction

TorS

TorT

TMAO

TorT

TorR

TMAO

TorR*

pTorCAD RFP

BBa_K1086000-------- Ba_K1086002 -------

‘

Trimethylamine-N-OxideTMAO: Construction

TorS

TorT

TMAO

TorT

TorR

TMAO

TorR*

pTorCAD RFP

TorT + TMAO

2 TorT + 2 TMAO + TorS

Trimethylamine-N-OxideTMAO: Modeling

Trimethylamine-N-OxideTMAO: Results

TorCAD+RFP ----- TMAO

Fluorescence/Absorbance

Ischemia Modified AlbuminIMA: Introduction

Normal patient blood

(Cobalt added)

Ischemic patient blood

(Cobalt added)

AlbuminIMA

Ischemia Modified AlbuminIMA: Construction

pRCNA YFP

-------- Ba_K1086001 --------

Normal patient blood

(Cobalt added)

Ischemic patient blood

(Cobalt added)

AlbuminIMA

Ischemia Modified AlbuminIMA: Modeling

Ischemia Modified AlbuminIMA: Modeling

Albumin 90 µM

Ischemia Modified AlbuminIMA: Modeling

Cobalt 25 µM

Ischemia Modified AlbuminIMA: Results

RCNA+YFP ----- Cobalt

Free Cobalt Assay:

Fluorescence/Absorbance

Ischemia Modified AlbuminIMA: Results

Bovine Serum Albumin Assay:

Fluorescence

RCNA+YFP ----- Cobalt

Ischemia Modified AlbuminIMA: Results

RCNA+YFP ----- IMA+Cobalt

Ischemic and Non-Ischemic Mice Sera Assay:

Fluorescence

Non-Ischemic

Ischemic

Talking with specialistsHuman Practices

Doctor Antonio Luiz Pinho Ribeiro

ETHICS BIOSAFETY

Neuza Antunes Rodrigues

Interest over time. Web Search. Worldwide, 2004 - present.

Scientific disseminationHuman Practices

Interest over time. Web Search. Worldwide, 2004 – present

English x Portuguese

GoalsHuman Practices

Cardbio

Impact

Thinking

outside

the box

Talking

with

specialists

Collaboration

Scientific

dissemination

Scientific disseminationHuman Practices

Our team

Scientific disseminationHuman Practices

Our team

Our university

Scientific disseminationHuman Practices

Scientific disseminationHuman Practices

Our team

Our university

Public schools

Scientific disseminationHuman Practices

UFMG & Escolas

Scientific disseminationHuman Practices

Missions

1. Fuel from sunlight

2. Microplastic hazard on the oceans

3. Spoiled meat

4. Malaria and artemisin

5. Celiac disease

6. Space exploration

7. Our own project

The Brickard Game

Scientific disseminationHuman Practices

Our team

Our university

Public schools

Our state

Scientific disseminationHuman Practices

Scientific disseminationHuman Practices

Our team

Our university

Public schools

Our state

Brazil

Scientific disseminationHuman Practices

Website

Scientific disseminationHuman Practices

Wikipedia

facebook.com/igemufmg

twitter.com/igemufmg

Scientific disseminationHuman Practices

Video aboutSynthetic Biology

Newspaper

GoalsHuman Practices

Cardbio

Impact

Thinking

outside

the box

Talking

with

specialists

Collaboration

Scientific

dissemination

Thinking outside the boxHuman Practices

Hackspace

Thinking outside the boxHuman Practices

Our song!

GoalsHuman Practices

Cardbio

Impact

Thinking

outside

the box

Talking

with

specialists

Collaboration

Scientific

dissemination

CollaborationHuman Practices

CollaborationHuman Practices

Jamboré – Meeting of Brazilian teams

Achievements

Assembly, registry and characterization of new biobricks:

RCNA + YFP (BBa_K1086001)

TorCAD (BBa_K1086000)

TorCAD+ RFP (BBa_K1086002)

Modeling of our systems.

Expand the knowledge about synthetic biology in our University,

our State and our Country.

Thanks

Sponsors

Other supporters

Laboratório de Genética Bioquímica Laboratório de Sinalização Celular e NanobiotecnologiaLaboratório de Genômica e Genética de Parasitos Laboratório de Imunoquímica de Toxinas NaturaisLaboratório de Sistema de InformaçãoLaboratório de Angiogênese

Professor Gastone CastellaniProfessora Andréa Mara MacedoNeuza Antunes RodriguesJuliana PimentaRosalvo Fogaça –UFPRIzabella Pena Neshich – UNICAMPDoutor Antonio Luiz Pinho RibeiroAndré Luis Martins Reis – Johns Hopkins

Thanks

Crowd funding donators

Denis RogerPedro Henrique Nascimento de AguiarFilipe Silva VillelaSabrina LimaMarina DinizDiego Pessoa SantosGraciela Kunrath LimaBel BitarIsabela F. PratesMarcela Segatto do CarmoHenrique TeichmannNicole SchererWagner ArbexGlória FrancoNaíla Barbosa da Costa

Fernanda Santos FreitasPedro E. C. VieiraFrancislon Silva de OliveiraLucas Antunes de Siqueira CostaPedro Pinho TeixeiraJarbas RohrMainá BitarLeonardo Lucas Carnevalli DiasLuandy BaiãoWesley Júnio da SilvaMichele AraújoNeuza AntunesJuliano LealCeres Luciana AlvesMaíra FreireAmanda Campelo

Thank you!