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Nanoparticle Mediated Tumor Vascular Disruption: A Novel

Strategy in Radiation TherapySijumon Kunjachan, Alexandre Detappe, Rajiv Kumar, Thomas Ireland,

Lisa Cameron, Douglas E Biancur, Vincent Motto-Ros, Lucie Sancey, Srinivas Sridhar, G. Mike Makrigiorgos, and Ross I Berbeco.

Presented by,Mr. Syed Baseeruddin AlviRollNo. BM16RESCH11005

Subject: BionanotechnologyDepaetment: BMEIndian Institute of Technology Hyderabad Date: 15-09-

2016

Journal club discussion

It is challenging to treat deeply seated non resectable tumors by radiation therapy.

Problem Statement

• Inefficiency in passive targeting (EPR) of fully developed tumors

• Hindrance posed by dense fibrous microenvironment of solid tumors

• Off target toxicity due to radiation

A strategy to intensify tumor damage and spare the healthy tissue is desirable.

A dual-targeting strategy using vessel-targeted-radiosensitizing gold nanoparticles and

conformal-image guided radiation therapy to specifically amplify damage in the tumor

neoendothelium

Innovation

Experiment & Resultsa b c

d

(a) Conjugated AuNP (b) DLS of naked AuNP & conjugated NP (c) Absorption & fluorescence spectra of the same (650/668nm) (d) TEM image of conjugated NP (e)

Confocal imaging with RGD:AuNP (red) and FITC-Dextran (green) shows a high degree of vascular damage in targeted NP (+IR)

fe

In v

ivo

anal

ysis

g

(g) SARRP images of panc-1 tumor xenograft (r-100%, y-60%, g-10%) (h) bio distribution by ICPMS

(I-L) laser induced breakdown spectroscopy of tumor tissue post

NP administration

h

Au (p

pm) p

er g

m o

f tis

ue

This dual-targeting strategy could help maximize the therapeutic index by Increasing tumor damage Reducing the amount of radiation dose needed and therefore

limiting collateral damage to healthy tissues.

Conclusion

In v

ivo

anal

ysis

Thank you