Squamous Cell Lung Carcinoma Looking for tell-tale signs Nacho

Caballero

P2-N

P1

P4 P3

P2

P2

Normal

Intermediate Tumor

P2-N

Human height follows a normal distribution

Distribution of the number of reads that align to each gene

# reads

RNA Seq data can be modeled using a

negative binomial distribution

# reads

- 0 + Log Fold Change

Significance High

Low

Genes with significant count differences between

normal and intermediate samples

Normal Intermediate Tumor

Patient 1 Patient 2 Patient 3 Patient 4

100K counts 10K counts 1K counts 100 counts 10 counts 1 counts

CDON counts are significantly smaller in intermediate than in normal

Patient 1 Patient 2 Patient 3 Patient 4

71 1267

Intermediate vs

Normal

Tumor vs

Normal

Number of significant genes

226 1041 217

3 patients 4 patients

43 28 15

3 patients 4 patients

Number of significant genes

Intermediate vs

Normal

Tumor vs

Normal

Interpreting the tell-tale signs