Semen Evaluation

In Animals

By

Dr.Sulake Fadhil Abbas

1-Volume

The graduated collection tube use to

determine the volume of ejaculate to the nearest

0.1ml, just after collection.

2-Hydrogen ion concentration (pH) and color: Values of seminal pH determined by using pH paper.

3-Motility a)Mass motility A drop of fresh semen deposit on a warm glass slide (37°C) and its motility evaluate according to wave motion table under microscope (magnification 100x)

b) Individual motility Individual sperm motility evaluate by depositing a few drops of diluted semen by normal saline on a warm glass slide (37°C), covered by cover slip. Evaluation make according to progressively foreword motility.

4-Dead sperms percentage: A drop of fresh semen mix with one drop of eosin stain then with two drops of nigrosin stain and smear make and dried. The slide examine under microscope (magnification 400x), uncolored sperm head (white) is considered as life sperm, while pink head (colored) due to dead sperm. 5-Abnormal sperms percentage: Determination of abnormal sperm percentage make using the same smear of live and dead.

6-Sperm concentration:Made by adding 0.1ml of fresh semen to 19.9 ml of sperm counting solution (2.9 sodium citrate +few drops of eosin to facilitate distinguishing of sperm under microscope +Mercuric chloride (HgCl2) as killing agent for sperm). The solution components mix for 3 minutes, after that using Neubauer chamber (RBCs square) sperms were counted to determine sperm concentration using the following equation:

C=n X200 X 400 X 10 X1000/ 80-n=No .of sperm in five square.-200= converse dilution rate. -80=No .of small square in five large squares.-10= converse depth between slide and cover slide.-400= converse square area of cover slide.