Guide:Dr. K. N. Gujar .h

Co guide : Mrs. V. M .Gambhire.

Presented By:Mr. Mahesh A. Marathe.

M. Pharm. (Pharmaceutics Dep.)

Sem. III

Sinhgad College Of Pharmacy ,Pune

Marketed product

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First photosynthetic life from nature(3.6 billion years ago) .

Spirulina is Blue Green Algae (Cyanobacteria).

Produce higher all life forms to evolve and produce oxygen.

Spirulina is rich in proteins , vitamins and minerals.

One gram of Spirulina proteins equals to 1 kg of assorted

vegetables.

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P. Saranraj* and S. Sivasakthi ,SPIRULINA PLATENSIS – FOOD FOR FUTURE: A

REVIEW, Asian Journal of Pharmaceutical Science & Technology Vol 4 | Issue 1 | 2014 | 26-

Spirulina is an interesting food rich in vitamins which can be

administered to children without risk.

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P. Saranraj* and S. Sivasakthi ,SPIRULINA PLATENSIS – FOOD FOR FUTURE: A

REVIEW, Asian Journal of Pharmaceutical Science & Technology Vol 4 | Issue 1 | 2014 | 26-

PHYCOCYANIN :

Water soluble blue pigment that gives Spirulina its bluish tint.

It is powerful water soluble antioxidants.

Spirulina plantesis is excellent source of phycocyanin.

Phycocyanin located in photosynthetic lamella in cytoplasm

membrane of Spirulina .

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1) Spirulina helpful as Anti stress , Anti fatigue nutrient .and

also beneficial against Anxiety and depression .

2) Spirulina also used as skin cream to promote faster wound

healing.

3) Spirulina useful for pregnant and nursing mother.

4) Spirulina also useful for menopause.(hormonal balance)

5) Spirulina use for Aging process and prevention of Diabetes type 2.

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Amha Belay, PhD* , The Potential Application of Spirulina as a Nutritional andTherapeutic Supplement in Health Management, The Journal of the American Nutraceutical

ANTICANCER F

O

O

D

I

N

D

U

S

T

R

Y

C

O

S

M

E

T

I

C

I

N

D.PROTECT AGAINST RENAL FAILURE

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Homogenization

In organic and inorganic acid

Freezing and Thawing

Cold Maceration

Sonication

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S.Sivasankari*, Naganandhini and David Ravindran ,”Comparison of Different Extraction methods for Phycocyanin Extraction and Yield from Spirulina platensis”,

Enzymatic method

Thermal shock method

In Sodium phosphate buffer

Other Methods…

S.Sivasankari*, Naganandhini and David Ravindran ,”Comparison of Different Extraction methods for Phycocyanin Extraction and Yield from Spirulina platensis”, Int.J.Curr.Microbiol.App.Sci (2014) 3(8) 904-909

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This method involves

crushing of cells using

mortar and pestle in

presence of acid washed

neutral sand using 50 mm

phosphate buffer at pH

6.8

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Thermic shock method

This method allowed pigment separation via density

gradient centrifugation with sucrose.

With the help of use of reverse phase chromatography it is

possible to achieve isolation up to 85% of phycocyanin.

Freeze thaw Method

This method use to achieve cell disruption via series of

freezing and thawing.

Freezing forms ice crystals and which expanded upon

thawing.

Verónica Cruz de Jesús1,et.al, Methods for Extraction, Isolation and Purification of C

phycocyanin: 50 years of Research in Review, International Journal of Food and

Nutritional Science, Published Date: June 15, 2016

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Enzymatic Method

3-7gm of algae suspended in 30 ml of 0.1 M Na-phosphate

buffer (pH 7.0) containing 100 microgram lysozyme (enzyme).

Shaking bath at 300c for 24 hrs

Enzymatic destruction of cell wall

Centrifuged for 1 hr at 10000 rpm

Remove cell debris and collect clear blue colour supernatant.

M. Muthulakshmi, A. Saranya, M. Sudha, and G. Selvakumar ,

Extraction, partial purification, and antibacterial activity of phycocyanin

from Spirulina isolated from fresh water body against various human

pathogens, J. Algal Biomass Utln. 2012, 3 (3): 7– 11

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Inorganic Acid Extraction

The procedure was carried out in the same way used in inorganic

acid extraction; in this case the wet biomass was treated with 1 M

of acetic acid at room temperature.

The wet biomass was treated with different concentrations of

hydrochloric acid (2, 4, 6, 8 and 12 M) in the proportion 5:1 (g

biomass: mL inorganic acid) and then left for 24 hours at room

temperature.

Organic acid extraction

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Biomass was added to an ultrasonic bath (50 kHz) for 40

minutes with solvent (phosphate buffer or water)

Sonication Method

S.Sivasankari*, Naganandhini and David Ravindran ,”Comparison of Different Extraction methods for Phycocyanin Extraction and Yield from Spirulina platensis”, Int.J.Curr.Microbiol.App.Sci (2014) 3(8) 904-909

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Conclusion

Freezing and thawing method yielded about 0.38 mg/g,

Homogenization using mortar and pestle yielded 0.06mg/g ,

Organic and inorganic acid treatment yielded up to 0.06 mg/g

and 0.28mg/g,

Sodium phosphate buffer and yielded 0.25mg/g ,

Sonication yielded 0.02mg/g of phycocyanin.

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Drying methods were resulted in approximately 50% loss of

Phycocyanin.

Fresh wet biomass was suitable for phycocyanin extraction.

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Isolation of c- phycocyanin also done by combined chromato -

graphy techniques.

Cold maceration and freezing thawing method shows higher

efficiency than other methods.

It can be the excellent source of income generation for industry

Spirulina is excellent natural source of essential nutrients.

Spirulina has wide range of natural treatment for various

diseases and in research area for discovery.

Spirulina useful for athletes ,malnutrition ,cardiac diseases and

also as anti-inflammatory drug.

Yong Chang Seo 1, Woo Seok Choi 1, Jong Ho Park 2, Jin Oh

Park 2, Stable Isolation of Phycocyanin from Spirulina platensis

Associated with High-Pressure Extraction ProcessInt. J. Mol. Sci.

2013, 14, 1778-1787; doi:10.3390/ijms14011778

S.Sivasankari*, Naganandhini and David Ravindran ,”Comparison

of Different Extraction methods for Phycocyanin Extraction and Yield

from Spirulina platensis”, Int.J.Curr.Microbiol.App.Sci (2014) 3(8) 904-

909

M. Muthulakshmi, A. Saranya, M. Sudha, and G. Selvakumar ,

Extraction, partial purification, and antibacterial activity of

phycocyanin from Spirulina isolated from fresh water body against

various human pathogens, J. Algal Biomass Utln. 2012, 3 (3): 7– 11

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P. Saranraj* and S. Sivasakthi ,SPIRULINA PLATENSIS –

FOOD FOR FUTURE: A REVIEW, Asian Journal of

Pharmaceutical Science & Technology Vol 4 | Issue 1 | 2014 | 26-

33.

Verónica Cruz de Jesús1, Gabriel Alfonso Gutiérrez

Rebolledo2,et.al, Methods for Extraction, Isolation and Purification

of C-phycocyanin: 50 years of Research in Review, International

Journal of Food and Nutritional Science, Published Date: June 15,

2016

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