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Introduction
Umaira Kanwal
Roll No.2021Department Of Biotechnology and Bioinformatics
Government College University Faisalabad
Dengue Virus
The dengue virus (DENV) in one of five serotypes is the
cause of dengue fever. It is a mosquito-borne single
positive-stranded RNA virus of the family Flaviviridae;
genus Flavivirus. All four serotypes can cause the full
spectrum of disease
Dengue Proteins
Structural Proteins
Envelop
Capsid
Non-Structural Proteins
Seven Proteins:
NS1, NS2A,NS2B, NS3, NS4A, NS4B, and NS5,
Membrane
NS3: Enzymetic
Reaction and Viral
Replication
Dengue Virus Structure
It has also been reported that NS3 has DNA
unwinding activity. Despite the high mutation rate
of DENV, some residues that actively participate
in viral replication remain conserved. These
conserved residues can be important in
developing specific antiviral agents and inhibitors
against DENVs.
Methodology
Kainat Arif
Roll No. 2027Department Of Biotechnology and Bioinformatics
Government College University Faisalabad
127 sequence
OF Dengue NS3 Protein
(NCBI)
30 seqof
DENV1
31 seq of DENV4
33 seqof
DENV2
31 seqof
DENV3
Peptides designing for potential peptide vaccine
127 seq’NCBI
Consensus SeqOf Each
Serotype (CLC work bench)
Consensus of all serotype
Global consensus Seq.
Short peptid Oh
highly conserved
Phylogenetic tree analysis
125 sequence
first aligned in CLCWorkbench,
The UPGMA method is based on
pairwise similarity/
dissimilarity distance matrix.1
Evolutionary TREE
NS3 Result and Discussion
Athar Hussain
Department Of Biotechnology and Bioinformatics
Government College University Faisalabad
NS3 Protein
• Two domains ,serine protease (protease domain ->six β-strands(two β-barrels formed by residues 1–180 ) and NTPasehelicase(Involve in Viral replication)
• Cofactor NS2B wraps around the NS3 protease domain and becomes part of the active site
• Second largest protein of DENV genome.
Mutation in residues (Y163
and G164) to alanine can
completely abrogate the
enzyme activity.
Helicase Domain
Consensus Sequence Domain encoding
Helps in processing of the
NS3 poly-protein precursor
into mature proteins
The consensus sequence alignment shows that:1.Residue L226 to L245 encodes the P-loop NTPase superfamily. It has two motifs [Walker A, GK(S/T), and Walker B, Dex (D/H)], designing antipeptidesagainst the Walker A/Walker B region can significantly reduce RNA processing efficiency.
Suggestions
2.I349 to R357 encodes helicase DEAD-box domain. Designing Antipeptides against
DEAD-Box can control Viral activites
3.The peptide string from 465 to 473 (QRR267 GR469 XGRN) is the part of the
helicase domain , mutation in R467 and R469 that will reduce the activity of
helicase4.Mutation in residues (Y159 and G160) to alanine can completely
abrogate the enzyme activity