Mahendra Rao- NIHCRM

New Uses of Cord Blood

The Cord Blood Industry

Hybrid • Korea

Other stem cells

MSC and Cord

Banking other cells

Making iPSC

HSC derivatives

• Public cord blood units are HLA typed • FACT offers accreditation services • US FDA has required licenses for public banks and we

expect this to be extended to private banks • The use of cord blood in children has exceeded the use of

bone marrow in children

Private eg: • Beike • China Cord

Blood • CBR

Public eg: • NYCBB • Carolinas • ClinImmune

• Private cord blood banks have proliferated and prices for storage have dropped.

• Most private large cord blood are in Asia and there is some consolidation and the average units stored in banks is in the range of 100K units

• Likely 50% of the units are too small for therapeutic use

Expanison of CBB’s

T cells, NK cells etc.

Potential other uses of cord blood

1: Expansion of cord blood 2: Identifying other stem cell populations a: MSC b: Hemangioblast c: Endothelial cells d: Differentiated blood products 3: Storing and using Placental tissue and cord 4:: Using infrastructure to store and process other cells 5: Making iPSC 6: Transdifferentiating to other cell types

Name/Website ViviCells

International, Inc. http://www.vivicells.

com

MEDIPOST http://www.medi-

post.com/

Lifeline Cord Blood Bank

Thai StemLife http://www.thaistemlife.co.

th

Vita 34 AG http://www.vita34.

de/

Cord Blood America http://www.cordblood-

america.com

Anthrogenesis Inc.

Eticur GmbH http://www.eticu

r.de

Cordlife Group Limited

http://www.cordlife.com

Meko Stem http://www.mekoste

m.com/

MAZE Cord Blood Laboratories

http://www.mazecordblood.com

Community Blood Services

Public Bank http://www.communitybloodserv

ices.org

The Institute For Transfusion Medicine Clinical Services,

ITxMCS, Cord Blood Services http://www.givcord.org/

StemCyte Inc. http://www.stemcy

teinc.com

Cord Blood Bank of Canada Inc

http://www.cordbloodbankofcanada.com

AssureImmune LLC

http://www.assureimmune.co

m/

FamilyCord http://www.famil

ycord.com/

CReATe Cord Blood Bank

http://www.createcordbank.com

MiracleCord, Inc http://www.miraclec

ord.com/

Karocell Tissue Engineering

http://www.karocell.com

NHS Cord Blood Bank

http://www.nhsbt.nhs.uk/

Insception http://www.insception.com

/

Smart Cells International

http://www.smartcells.com/

Cord Blood Registry http://www.cordblood.

com

BioE, Inc. http://www.bio

e.com

Future Health Biobank

http://www.futurehealth.co.uk

CRYOBANKS INTERNATIONAL,

INC. http://www.cryo-

intl.com National Stem Cell,

Inc. http://www.national

stemcell.com

Lazaron Biotechnologies (SA) Ltd

http://www.lazaron.co.za

StemLife Malaysia

http://www.stemlife.com

Celgene Corporation http://www.celgene.com

PacifiCord http://www.pacific

ord.com/

Cord Blood Solutions http://www.cordbloods

olutions.com/

Biohellenika http://www.biohe

llenika.ro

Healthbaby Biotech Co. Ltd

http://www.healthbaby.hk/

Cryo-Cell International, Inc. http://www.cryo-

cell.com

NeoCells http://www.neocells.

com/

Lifebank http://lifebank.com/

StemOne Biologicals Pvt.

Ltd. http://www.ste

mone.co.in

Cells For Life http://www.cellsforlife.com

/

Progenics Cord Blood Cryobank

http://www.progenicscryobank.com

Canadian Cord Blood Registry

http://www.ccbr.ca

BioLife Solutions Inc

http://www.biolifesolutions.co

m

Healthcord http://www.healt

hcord.com/

Cryo-Cell Mexico http://www.cryo-

cell.com.mx/

NeoStem, Inc. http://www.neostem

.com

LifebankUSA, Inc. http://www.lifebankusa.c

om

Stem Cell Institute

http://www.stemcell.co.jp/

China Cord Blood Corporation

http://www.chinacordbloodcorp.com

Relicord http://www.relicor

d.com/

Biosafe http://www.biosafe.ch

Bionet http://www.babybanks.com/2009/bionet-en

Héma-Québec http://www.hema-quebec.qc.ca

Cryocord http://www.cryocord

.com.my

Netcells Cryogenics http://www.netcells.

co.za/

LifeCord International http://english.lifecord.co.

kr

Stem Sciences, Inc

http://www.stemsciences.com/

CorCell, Inc. http://www.corcell.com

Saneron CCEL Therapeutics, Inc.

http://www.saneron-ccel.com

Alpha Cord http://www.alphacord.c

om/

CordBank http://www.cor

dbank.co.nz

HemaStem http://www.hem

astem.com/

Cryolife http://www.cryolife.

com.hk

New England Cord Blood Bank

http://www.cordbloodbank.com

Lifeforce Cryobanks http://www.lifeforcecryo

banks.com

ViaCord http://www.viac

ord.com/

Victoria Angel Registry of Hope Public Cord Blood

Bank http://www.cellsforlife.com

/victoriaangel/

ScienCell Research http://www.scienc

ellonline.com

Anthony Nolan Trust Cord Blood Bank

http://www.anthonynolan.org/

CordBancUSA

D-Finitive Cell Technologies

Cryo-Save http://www.cryo-

save.com

New Jersey Cord Blood Bank

LifeLine Cryogenics http://www.lifelinecryog

enics.com

SEWON Cellontech

http://www.cellontech.com

Virgin Health Bank http://www.virginhealthban

k.com/

Securacell http://www.secura

cell.com

Alberta Cord Blood Bank

http://www.acbb.ca/

Elie Katz Umbilical Cord Blood Program http://www.communitybloodservices.org/cb_

ekucbp.php

Cryosite http://cordblood.

cryosite.com/

Cord Blood Companies

ViviCells International, Inc. http://www.vivicells.com

MEDIPOST http://www.medi-post.com/

Lifeline Cord Blood Bank

Thai StemLife http://www.thaistemlife.co.th

Meko Stem http://www.mekostem.com/

MAZE Cord Blood Laboratories http://www.mazecordblood.com

Community Blood Services Public Bank

http://www.communitybloodservices.org

The Institute For Transfusion Medicine Clinical Services, ITxMCS, Cord Blood

Services http://www.givcord.org/

MiracleCord, Inc http://www.miraclecord.com/

Karocell Tissue Engineering http://www.karocell.com

NHS Cord Blood Bank http://www.nhsbt.nhs.uk/

Insception http://www.insception.com/

National Stem Cell, Inc. http://www.nationalstemcell.com

Lazaron Biotechnologies (SA) Ltd http://www.lazaron.co.za

StemLife Malaysia http://www.stemlife.com

Celgene Corporation http://www.celgene.com

NeoCells http://www.neocells.com/

Lifebank http://lifebank.com/

StemOne Biologicals Pvt. Ltd.

http://www.stemone.co.in

Cells For Life http://www.cellsforlife.com/

NeoStem, Inc. http://www.neostem.com

LifebankUSA, Inc. http://www.lifebankusa.com

Stem Cell Institute http://www.stemcell.co.jp/

China Cord Blood Corporation http://www.chinacordbloodcorp.com

Netcells Cryogenics http://www.netcells.co.za/

LifeCord International http://english.lifecord.co.kr

Stem Sciences, Inc http://www.stemsciences.c

om/

CorCell, Inc. http://www.corcell.com

New England Cord Blood Bank http://www.cordbloodbank.com

Lifeforce Cryobanks http://www.lifeforcecryobanks.com

ViaCord http://www.viacord.com/

Victoria Angel Registry of Hope Public Cord Blood Bank

http://www.cellsforlife.com/victoriaangel/

New Jersey Cord Blood Bank

LifeLine Cryogenics http://www.lifelinecryogenics.com

SEWON Cellontech http://www.cellontech.co

m

Virgin Health Bank http://www.virginhealthbank.com/

Vita 34 AG http://www.vita34.de/

Cord Blood America http://www.cordblood-america.com

Anthrogenesis Inc.

Eticur GmbH http://www.eticur.de

Cord Blood Companies

StemCyte Inc. http://www.stemcyteinc.com

Cord Blood Bank of Canada Inc http://www.cordbloodbankofca

nada.com

AssureImmune LLC http://www.assureimm

une.com/

FamilyCord http://www.familycord.com/

CReATe Cord Blood Bank http://www.createcordb

ank.com

Smart Cells International http://www.smartcells.com/

Cord Blood Registry http://www.cordblood.com

BioE, Inc. http://www.bioe.com

Future Health Biobank http://www.futurehealth.co.uk

CRYOBANKS INTERNATIONAL, INC.

http://www.cryo-intl.com

PacifiCord http://www.pacificord.com/

Cord Blood Solutions http://www.cordbloodsolution

s.com/

Biohellenika http://www.biohellenik

a.ro

Healthbaby Biotech Co. Ltd http://www.healthbaby.hk

Cryo-Cell International, Inc.

http://www.cryo-cell.com

Progenics Cord Blood Cryobank

http://www.progenicscryobank.com

Canadian Cord Blood Registry http://www.ccbr.ca

BioLife Solutions Inc http://www.biolifesoluti

ons.com

Healthcord http://www.healthcord.com

Cryo-Cell Mexico http://www.cryo-

cell.com.mx/

Relicord http://www.relicord.com/

Biosafe http://www.biosafe.ch

Bionet http://www.babybanks.

com/2009/bionet-en

Héma-Québec http://www.hema-

quebec.qc.ca

Cryocord http://www.cryocord.co

m.my Saneron CCEL Therapeutics,

Inc. http://www.saneron-

ccel.com

Alpha Cord http://www.alphacord.com/

CordBank http://www.cordbank.c

o.nz

HemaStem http://www.hemastem.com

Cryolife http://www.cryolife.co

m.hk

ScienCell Research http://www.sciencellonline.c

om

Anthony Nolan Trust Cord Blood Bank

http://www.anthonynolan.org/

CordBancUSA

D-Finitive Cell Technologies

Cryo-Save http://www.cryo-

save.com

Securacell http://www.securacell.com

Alberta Cord Blood Bank http://www.acbb.ca/

Elie Katz Umbilical Cord Blood Program

http://www.communitybloodservices.org/cb_e

kucbp.php

Cryosite http://cordblood.cryosite.com

Cord Blood Companies (con’t)

Blood collection and processing devices

Derivation of an iPSC

culture

Reprogramming

Candidate ESC like line

Select clones

Adult cells

Identify iPSC clones from partially programmed

Differentiated cell type

Grow propagate and bank

Around 1-5M

Multiple strategies with advantages and disadvantages

Around 21-28 days

1-6 rounds of infection Around 50-150 colonies

10-30 clones picked

Pick 3 to be safe per parent line

ESC like line

Test for differentiation

Test for pluripotency

Around 8 weeks-12 weeks

Around 5 weeks for EB stage

Around 6 weeks

Around 6 weeks

Why source from cord blood banks 1: Tissue sourcing is well organized and validated

2: HLA typing data is already being collected by banks

3: Existing samples can be used without compromising their ultimate use

4: A renumeration model already exists

5: Zerofootprint methods work well with cord blood cells

6: A Xenofree media and protocol has been developed so clinical grade iPSC lines can be made

7: Cord blood is the youngest source of stem cells one may obtain reliably and easily

8: Stem cells in general have specific mechanisms to maintain genomic integrity , delay senescence and protect against transformation.

9: The process can be easily extended to blood banks and marrow derived CD34 cells using the same infrastructure

10: Somatic memory and differentiation bias may work in our favor for early therapeutic efforts

11: May revive the cord blood banking business

Table 1: The relative advantages of making iPSC from either a private or a public cord blood bank are listed.

iPSC from blood or cord blood

•Fresh Harvest

•CD34+ cells or mononuclear fraction

•Confirm consent •Data Anonymization Issues •HLA typing data

•iPSC generation

•50,000 cells •Sendai •Electroporation •Vectors •IP issues •96 well format •Media and timeline

•At time of use of unit

•From designed bag or tubing •/ Non Ficoll process

•Cord Bank

•Receive at iPSC site

•Shipping •Tracking •Identity

•iPSC testing •Pluripotency •Differentiation •Karyotype

•Ship and store

Thank You Collaborators