METHOD OF DNA REPAIR LINKED TO HIGHER LIKELIHOOD OF GENETIC MUTATION AND UNLOCKING THE SECRETS OF DNA

Mª Alejandra Ramirez RuizMedicine Student 3rd Semester

FOLDING

FOLDING

INTRODUCTION

Neutron Scattering is a

new techniques that will permit advance in DNA

investigation

Scientists have discovered that

the cell have mechanisms that can acts against

it.

METHOD OF DNA REPAIR

LINKED TO HIGHER

LIKELIHOOD

OF GENETIC MUTATIONSCIENCEDAILY (FEB. 15, 2011)

METHOD OF DNA REPAIR LINKED TO

HIGHER LIKELIHOOD OF GENETIC

MUTATION

A method by which cellsrepair breaks in their DNA,known as Break-inducedReplication (BIR), is up to2,800 times more likely tocause genetic mutation thannormal DNA synthesis.

Representation of a DNA ligase repairing double-stranded

METHOD OF DNA REPAIR LINKED TO

HIGHER LIKELIHOOD OF GENETIC

MUTATION

When BIR occurs, instead of using a "bandaid" to repair, the broken piece invadesanother chromosome and initiatesreplication which happens at the wrongplace and at the wrong time.

When the cell use this method accuracy islost.Dna Damage

METHOD OF DNA REPAIR LINKED TO

HIGHER LIKELIHOOD OF GENETIC

MUTATION

There are at least four changes to the replication machinerythat might occur to create a perfect storm or synergy thatmake BIR repair so mutagenic, and the mutagenesis causedoccurs in surges.

This study could help to prevent mutations that can causecancer.

Cancer cells

OBSERVATION

Contrary to the common

idea that all repair

mechanisms are

beneficial, the BIRrepair

system brings fatal

consequences in the

replication process generated

by an excess concentration

of nucleotides.

It is clear that not all

mutations are

harmful because

evolutionary

processes also occur

as a result.

UNLOCKING THE

SECRETS OF DNASCIENCEDAILY (JAN. 28, 2011)

UNLOCKING THE SECRETS OF DNA

Neutron scattering gives information about thecorrelation between base pairs duringdenaturation, which is not possible using othertechniques.

DNA components: Form andstructure determination by neutronscattering

It is used to measure thecharacteristic size of the denaturedregions as the temperature ischanged

A range of temperatures, willcause that the bonds betweenbase pairs break, ordenature, causing the twostrands of DNA to separate.

The experiment could onlymeasure the first stage: when thestrands become 50% denaturedthey are too floppy to remainordered. The DNA sampleliterally falls to pieces.

UNLOCKING THE SECRETS OF DNA

The experiment follows from the pioneering work ofRosalind Franklin, who showed that x-ray scatteringfrom DNA fibres would give structural information.Based on her work, James Watson and Francis Crickdeduced the well-known double helix structure of DNAin 1953.

UNLOCKING THE SECRETS OF DNA

James Watson and Francis Crick

OBSERVATION

The use of advanced techniques for the

study of DNA, allows to continuous learning

about the most important molecule in

molecular biology.

Know in detail the formation and operation

brings great advances, not only in the

field of medicine, but also in the developing

world of technology and information.

MEDICAL UTILITY

MEDICAL UTILITY

Understand the workings of DNA allows forbreakthroughs in medical issues such as geneticrecombination, inheritance, mutations, congenitaldiseases, repair mechanisms, among others.

Knowing the molecule that contains all the geneticinformation is like deciphering the map of life.

Find different mechanisms to counteract the effect ofdiseases that have a direct attack in the genome

MEDICAL UTILITY

MEANS

It’s useful to employ newtechniques to investigate thegenome, and also explore somemechanisms of the cell that cango against their preservation.

Science can develop strategiesabout them and could act againstwhich represents a major threathuman such as cancer.

MEDICAL UTILITY

BIBLIOGRAPHY MARTINEZ S., Lina María. Biología molecular. 6. ed.

Medellín : UPB. Fac. de Medicina, 2009. 56 p.

Public Library of Science (2011, February 15). Method ofDNA repair linked to higher likelihood of genetic mutation.ScienceDaily. Retrieved February 19, 2011, fromhttp://www.sciencedaily.com/releases/2011/02/110215191625.htm

Institut Laue-Langevin (ILL) (2011, January 28). Unlockingthe secrets of DNA. ScienceDaily. Retrieved February18, 2011, from http://www.sciencedaily.com­/releases/2011/01/110124120848.htm

¡THANKS!