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INTRODUCTION TO DRINKING WATER QUALITY A Layperson’s Guide to Water Quality, Waterborne Diseases and Water Quality Monitoring A Publication by

Introduction to Drinking Water Quality: A Layperson’s Guide to Water Quality, Waterborne Diseases and Water Quality Monitoring

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INTRODUCTION TO DRINKING WATER QUALITY

A Layperson’s Guide to

Water Quality, Waterborne Diseases

and Water Quality Monitoring

A Publication by

CONTENTSIntroduction

Why Water Quality

“Improved” Water vs. “Safe” Water

Global Health Crisis

Waterborne Diseases

How Water Becomes Contaminated

Corrective Actions to Improve Water Quality

UN Sustainable Development Goal 6 (SDG 6)

Water Quality Monitoring

Aquagenx Compartment Bag Test (CBT)

Aquagenx Procurement Programs for SDG 6

Conclusion

2

INTRODUCTION

Poor water quality is a global health

crisis with many layers of complexity.

Not everyone who must help remedy

this monumental problem is a

scientist, or policy maker, or water,

sanitation and hygiene expert, or

public health professional.

However, everyone involved with this

challenge at every level must have

some understanding of the subject and

science behind it.

3

Source: justgiving.com

4

This booklet provides basic information to help people make intelligent decisions about safe drinking water.

It’s also an introductory resource for United Nations Sustainable Development Goal 6. All stakeholders for targets in this goal, including people who aren’t scientists or water professionals, need an elementary understanding of water quality.

AquagenxFebruary 2016

“More people die from unsafe water than from all forms of violence, including war…These deaths are an affront to our common humanity, and undermine the efforts of many countries to achieve theirdevelopment potential.”

Ban Ki-moonUnited Nations2012

“The quality of drinking-water is a powerful environmental determinant of health. Assurance of drinking-water safety is a foundation for the prevention and control of waterborne diseases.”

“Water and sanitation are at the very core of sustainable development, critical for thriving people, planet and prosperity.”

6

Safe and sufficient water supplies are mandatory for the health and well-being of humans and ecosystems, and for social and economic development.

The United Nations says safe water is a fundamental human right.

7

Source: Intentional Living

Throughout much of the 20th

century, water scarcity in developing countries – insufficient water quantity – was the focus of water challenges.

Billions of dollars were spent on improving access to water supplies and services.

8

Source: Rajnikant V/s

9

Water quantity is still an enormous problem in much of the developing world.

However, water quality is just as important as water quantity.

Source: preserveourwaters.weebly.com

All the investment in the world to improve water access is wasteful if the water quality in those sources is contaminated, causing death and disease.

Water quality has rightfully become a global concern.

10

Source: Sri Lanka Sunday Observer

11

Despite the growing concern, water is rarely monitored for qualityin the developing world.

Source: ChildFund International

Due to the achievements ofthe UN Millennium Development Goals and the work of NGOs, governments and other entities, there has been good progress toward safe drinking water in developing countries.

12

13

91% of the global population now uses an improved drinking water source

2.6 billion people have gained access to an improved drinking water source since 1990

The UN Joint Monitoring Program’s definition of “improved” water refers to the construction of a source – if the source is protected in some way.¹

It does not refer to the quality of the water.

¹ http://www.wssinfo.org/definitions-methods/

17

Billions of dollars have been spent on improving water sources, but there is recognition that an improved water source does not always equal safe water.

Water quality testing studies on improved water sources reveal those sources contain a high level of fecal contamination.

18

Source: MaaMatiManush.tv

World Health Organization and UNICEF Joint Monitoring Program

663 million people - 1 in 10 - lack

access to safe water

20

World Health Organization

1 billion people lack access to an

improved supply

2 million annual deaths attributable to unsafe water, sanitation and hygiene

21

Centers for Disease Control and Prevention

780 million people do not have access to an

improved water source

Estimated 800,000 children younger than

5 years of age die from diarrhea each year

22

United Nations

About 3.5 million deaths related to

inadequate water supply, sanitation and hygiene occur each year, predominantly in developing countries

23

In 2015, the World Economic Forum said the water crisis is the #1 global risk based on impact to society as a measure of devastation.

In 2016, the World Economic Forum ranked the water crisis as the top global risk to industry and society over the next decade.

24

Unsafe drinking water is especially tragic because the crisis is preventable.

25

Source: Rochester Institute of Technology

What is lurking in water that causes such tragedy?

Four types of organisms transmit waterborne diseases…

27

Source: MAD Water

The greatest waterborne riskto health comes from the transmission of fecal pathogens.

Fecal coliforms are a group of total coliforms found in the intestines and feces of humans and warm-blooded animals.

29

In any given sample of drinking water, the concentration of pathogens might be small, but the number of different pathogens is very large.

It’s impossible and too expensive to test for all pathogens in drinking water. Instead, we look for indirect evidence of pathogens by testing for indicator organisms.

30

Source: Sisters of Notre Dame

E. coli is a bacteria that is the most reliable indicatorof fecal contamination and pathogens in water.

Why is that?

31

32

E. coli is generally not found growing and

reproducing in the environment.

It has to be introduced into the environment via the feces of humans and warm-blooded animals.

At the Source

34

o Leaking septic tanks and latrineso Contaminated surface water run-off entering

wells, springs, other sourceso Wells with defective casings, linerso Collecting water with unwashed hands and/or

dirty containerso Objects falling into the well/sourceo Animals using the same sourceo Open defecation

At the Household Level

35

o Transporting water from the source to house in dirty containers

o Storing water in open and/or dirty containerso Handling water with dirty hands or utensils

Natural Disasters and Emergencies

36

o Via disaster itself such as flood waterso Poor sanitation and hygiene amongst

displaced populations, such as in refugee camps

Ensuring safe water supplies after a disaster is one of most important public health requirements to prevent the spread of waterborne diseases, along with adequate sanitation.

Water quality testing for E. coli following a disaster is critical.

37

38

Water sources that were once clean can become recontaminated.

Most waters are more vulnerable to E. coli contamination during and shortly after precipitation events and especially when there is flooding. Water sources can also become recontaminated in the ways described on pages 34-36.

Source: Reuters

40

When contaminated water is found, it is best to take actions to identify the reasons for the contamination by doing a sanitary assessment or sanitary inspection.

See the following examples:

www.who.int/water_sanitation_health/hygiene/emergencies/fs2_1.pdfwww.who.int/water_sanitation_health/dwq/wsp170805AppC.pdficeh.uws.edu.au/pdf_files/water_SanitaryInspection_Annex.pdfwww.lboro.ac.uk/well/resources/technical-briefs/50-sanitary-surveying.pdfhttp://www.who.int/water_sanitation_health/dwq/2edvol3c.pdf

41

Another recommended action is to take steps to correct any water point deficiencies, based on findings from the sanitary inspection/assessment. The actions depend on the types of deficiencies.

Some corrections or fixes are obvious, such as: restricting animals from accessing the water; adopting good sanitation and hygiene practices such as washing hands with soap; not allowing surface water sources for drinking water to be used for other purposes such as human or animal waste disposal or bathing.Source: pinterest.com

42

For unimproved waters that are typically of higher risks, treating the water is probably the best immediate intervention.

Treating water can be done at the water source point (where the water is collected) or at the point of use in the home (household water treatment).

Source: Wikipedia.com

The United Nations Sustainable Development Goals pick up where the Millennium Development Goals left off in 2015. Sustainable Development Goals are the 2030 Agenda.

Sustainable Development Goal 6 (SDG 6) is a dedicated

goal for water and sanitation. SDG 6 expands on the

Millennium Development Goals focus on drinking water and sanitation and covers the entire water cycle, including water management, wastewater and ecosystems.

44

45

Target 6.1: “By 2030, achieve universal and equitable access to safe and affordable drinking water for all.”

Target 6.3: “By 2030, improve water quality by reducing pollution, eliminating dumping and minimizing release of hazardous chemicals and materials, halving the proportion of untreated wastewater and increasing recycling and safe reuse globally.”

www.unwater.org/sdgs/a-dedicated-water-goal/en/

SDG 6 champions water quality. Targets involving water quality include…

46

In addition, SDG Goal 11 for safe cities references water and natural disasters…

Target 11.5 “By 2030, significantly reduce the number of deaths and the number of people affected and substantially decrease the economic losses relative to gross domestic product caused by disasters, including water-related disasters, with a focus on protecting the poor and people in vulnerable situations.”

47

SDG 6 is more ambitious than the Millennium Development Goals.

The new water targets are specific, measurable and action-oriented.

Source: umweltverschmutzung.wordpress.com

48

SDG 6 recognizes there is a need to test water at the household levelto both understand where water is unsafe and then to validate and audit various efforts to improve household and community water quality.

Accurate assessment of progresstoward safe water targets includes measuring E. coli concentrations in drinking water with accessible, precise tests.Source: waterencyclopedia.com

49

Measuring water quality with quantifiable data is crucial to ensure water sources and supplies remain safe to drink over time, and to understand how water quality changes over time.

This requires ongoing water quality monitoring.

One of the most important ways to eliminate the global water crisis of unsafe drinking water is to conduct ongoing water quality monitoring.

This should include routine bacteriological testing, and measuring water samples for the presence of fecal pathogens, especially E. coli.

51

Ongoing water quality monitoring must be done in a health risk-based framework.

We have to measure the presence of fecal organisms to know the extent of the health risk.

We have to obtain data to help make informed decisions about drinking water quality in order to take the most appropriate corrective actions.

52

Water quality should be monitored for safety over time, from the source or point of production, to the point of use and every point in between:

Source Tap Point of use Stored water Before treatment After treatment Households Non-households (schools, healthcare

facilities)

53

Source: Living Water International

Water quality monitoring has been extremely difficult to do in low resource, rural and disaster areas.

Part of the problem has been various water quality testing methods that aren’t convenient and practicalfor use in these settings.

54

Source: Lawrence Berkeley National Laboratory

Laboratories are often inaccessible or unavailable in remote locations and disaster areas.

Lab testing is expensive. You have to factor in sample transportation, sample analysis and processing and technicians’ fees.

Labs can also take a long time to generate and provide test results.

55

57

Complicated systems that are difficult for anyone to use

Require electricity, other resources, trained technicians

Require constant temperature control in an incubator

Require extra, bulky equipment that’s difficult to transport and use in-the-field

Require removable pieces of equipment that must be sterilized before re-use

Require a cold chain Do not analyze 100 mL sample Do not provide quantitative

test results

Roadblocks to on-site water quality monitoring other testing methods present

58

In order to easily monitor water quality no matter who is doing the testing and no matter where they are, simple, portable tests are needed.

Water quality tests must measure the presence of E. coli in water samples and generate quantified test results that provide data to make informed decisions about water safety.

60

The Aquagenx Compartment Bag Test (CBT) overcomes many of the roadblocks to on-site water quality testing.

It does exactly what the World Health Organization recommends for water quality testing, namely to test for E. coli bacteria in a 100 mL water sample.

The CBT does this in a remarkable way.

61

The CBT is optimized for on-site water quality testing in-the-field.

Lab–free Electricity-free Cold chain-free Small footprint - easy to pack and carry Simple to use - don’t have to be a scientist Easy color-change scoring method Quantified test results – Most Probable Number E. coli per 100 mL WHO recommended indicator organism and sample volume

62

It is the ideal water quality test for E. coli in low resource settings…

o Rural, remote locationso Developing countrieso Disaster and emergency areas

63

1. Collect 100 mL water sample2. Dissolve E. coli growth medium in sample for about 15 minutes3. Pour sample into compartment bag4. Incubate 24-48 hours depending on ambient temperature5. Score and record test results

The CBT is an every-person’s water quality test that takes just a few simple steps to complete.

64

In addition to its portability and simplicity, the CBT has two great features for

testing in remote, rural areas…

65

1

The CBT works at variable temperatures.

That’s because the enzymatic hydrolysis of our E. coli medium’s substrate, called X-Gluc for short, can occur during E. coli growth at a range of temperatures.

Other tests require precise, constant temperature control in an electricity-dependent incubator.

66

2

The CBT enables ambient temperature incubation at 25° Celsius and above, with test results in 24 hours.

If you’re testing in colder climates, you just need to maintain the sample anywhere between 25° - 44.5° Celsius and incubate for 48 hours. It takes longer for bacteria to grow in colder temperatures.

67

In colder climates, you can maintain CBT samples between 25° - 44.5°Celsius in several ways…

With the Aquagenx portable incubator,

or by other methods and techniques such as:

• Thermal coolers and bags• Poultry or reptile incubators• Keeping samples in warm rooms or near any type of heat source

68

Color changes in the CBT are easy to see.

Blue/blue-green color indicates presence of E. coli in a compartment.

Yellow/yellow brown color indicates absence of E. coli in a compartment.

The concentration of E. coli is estimated from the combination of positive and negative compartments in the bag.

69

To score your test results, line up your compartment bag to the Most Probable Number (MPN) Table in the CBT Instructions sheet.

70

Then, match the color sequence of your five compartments to one of the 32 color-coded rows on the MPN Table.

Each row gives you the Most Probable Number (MPN) of

E. coli in the 100 mL sample.

Our table also gives the Upper 95% Confidence Interval, which is the worst possible concentration of E. coli you would find if the same sample

was tested many times.

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Health Risk Category E. coli CFU per 100 mL

Safe <1

Intermediate Risk/Probably Safe 1-10

High Risk/Probably Unsafe >10-100

Very High Risk/Unsafe >100

The health risk categories in our MPN Table match the World Health Organization Guidelines for Drinking Water Quality, 4th Edition.

73

The combined data of MPN value and upper 95% Confidence Interval provides excellent information in order to make informed decisions about the health risk of water.

This is important in the WHO Intermediate health risk categories, because most decisions about water safety take place in these middle categories.

74

The CBT’s visual, color-change test results are also a powerful educational tool to help communities adopt better behaviors for sanitation and hygiene.

75

If you only used a Presence/Absence test for E. coli and scored a positive test result, you wouldn’t know if only one E. coli bacterium was in the sample or hundreds or more.

Isn’t it better to accurately measure the health risk of drinking water and obtain data and better information so you know more precisely how dangerous the sample might be?

76

The CBT has been tested extensively by independent parties and compared to other testing methods such as membrane filtration and IDEXX Colilert.

Time and time again, results are reliable and on par with more complicated, expensive and less portable water quality tests.

Visit this webpage to read papers, studies and articles on the CBT:

www.aquagenx.com/comparative-studies-and-cbt-performance-data

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“The CBT should be adopted for standard water quality testing practices and disaster response within the Red Cross/Red Crescent movement and used by all member societies working in a water supply capacity. Its speed, convenience and quantitative test results add value to water quality investigations and validate projects and programs. It is simple to use on-site in remote locations, and its color-change test results are an excellent way of raising water quality awareness for local communities.”

Stuart BryanAustralian Red Cross

Source: www.redcross.org.au

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“We will absolutely continue using the CBT in our projects to test for E. coli. It’s very easy to use, and even the village children in Tanzania learned to use it! The CBT is cheap, reliable, easy to transport and easy to understand. I recommend the CBT to anyone who does water quality testing in developing countries and low resource areas without access to electricity, incubators or labs.”

Pedro PiquerasEngineers Without BordersLos Angeles Professional Chapter

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Tens of thousands of CBTs are used by hundreds of customers around the world in dozens of countries.

Customers include:

NGOs - small, medium and large Humanitarian relief organizations Governments Private companies Universities

See more testimonials and customer stories on our website: www.aquagenx.com/testimonials

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Aquagenx is committed to helping all countries attain SDG 6 water quality targets.

We work with governments, NGOs and service providers to develop tailored, affordable, sustainable CBT Kit procurement programs that ensure convenience and success in meeting SDGs, especially in rural and low resource areas.

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Contact us so we can work with you to develop a CBT Procurement Program that meets your needs for SDG 6.

Contact Us

CBT Procurement Program contact form: www.aquagenx.com/cbt-procurement-program-for-sdg-6/

Contact Us

CONCLUSION

Many of us have heard the phrase,

“What isn’t measured isn’t managed.”

For safe water quality throughout the

world, that phrase is absolutely true.

We live in a data-driven world.

Obtaining data to ensure safe water for

everyone is a requirement, not a luxury.

Simple, convenient and effective tools

like the Aquagenx CBT are readily

available to help prevent the

devastating impact of contaminated

water on human lives.

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ADDITIONAL RESOURCES

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UN Water: http://www.unwater.org/

WHO Guidelines for Drinking Water Quality:

http://www.who.int/water_sanitation_health/dwq/guidelines/en/

U.S. EPA Water Quality Criteria: http://www.epa.gov/wqc