Safety Goal 1“No pain at the PC”

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proceed powerpoint by clicking mouse

As we saw in the video……Working with a PC can cause pain in

neck, arms, shoulders and wristsYou can do 3 things to prevent pain:

1 Adjust your work station

Everyone of us is built differentlySo the working station needs to be

tailored to the individual. This is also known as ergonomics >>>>>>>

>>getting it rightYou are in the correct position when the angle between upper and lower parts of your legs is about 90 degrees

The space between the edge of the chair and your knees should be about the size of your clenched fist

Your lower back should be supported

Adjust the height of the armrests; position them just under your elbows

Your monitor should be straight in front of you. The ideal distance between your eyes and the screen is 60 – 100 cm

Take some time to adjust everything!

2 Mind your posture

Sit straight up & keep your shoulders relaxed

Use mouse en keyboard with your elbows on the chairs armrests and with your wrists straight

3 Balance your workload

Alternate different types of tasks

Take regular breaks/ exercise, at least every 2 hours

• Walk over and speak to colleagues• Fetch your own cup of coffee• Use the stairs instead of the lift

Beware of working under too much pressure• You skip breaks and excercise• You suffer from stress• Muscular tension increases

A final remark on pain & the PC:

Work hard but be smart Understand the relationship between working at a PC and your

healthFor help with adjusting your workstation: call 85800 or email

facilitydesk.plant@wur.nl

18 slides to go

Safety Goal 2“Leave the lab alive”

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Proceed to learn more about the 2 dangers in the lab

Flammable

Irritating

Corrosive

Oxidizing

Explosive

Toxic

Danger 1: Chemicals ……...…..

Labcoat, goggles & gloves?

Working area?

Pipetting?

Flammables & Vapours?

Using the sink?

Right or wrong?

Reducing chemical risks

>>>getting it right

with the Fumehood

Use the fumehood when working with flammables or vapours

Make sure there’s no clutter inside the fumehood: it blocks the flow

Make sure the window is only about 1/3 open

Never put your head in!

Danger 2: Equipment………..

Under pressure and hot Sharp & cutting In other ways dangerous

Make sure you know how it is working: ask your lab supervisor

What when it goes wrong?

Fire?• Leave the room, alarm people• Follow the emergency signs

Chemicals in eyes or skin? • Shower or eye wash: rinse with lots of water• Make sure you know where they are and how to

use them!

Call (03174) 88112

More info on the leaflet near the door

A final remark on how to leave the lab alive

Know what you are doing!And know the emergency responsesFor more info on labsafety: ask your labsupervisor

11 slides to go

Safety Goal 3

“Bio-agents behind bars”

Genetically modified organisms (GMO) are genetically changed by men.

They can be any biological entity capable of replication or of transferring genetic material.

Some of them can be a treat to the ecosystem

Pathogens/ Quarantaine organisms are harmful. They can be viruses, fungi, bacteria or insects.

They can be genetically modified. They can be a treat to plants, animals or man.

Both GMO & Pathogens are to be contained on a specific bio safety level to make sure they don’t

harm us or escape to the environment.

Containment levels GMO & Pathogens fall into 4 risk groups: Each risk group should be contained at a specific level (Bio Safety Level, BSL)

BSL-1 BSL-2 BSL-3 BSL-4

Organisms practically unable to cause diseases

Moderate risk for individual/ limited risk for community

High risk for individual and community

High risk

Laboratory ML-IEscherichia coli

ML-IISalmonella spp.

ML-IIIMalaria

ML-IV

Climate room

PCM-I (infected plants)

PCM-II (infected plants)

PCM-III (infected plants)

PCM-IV (infected plants)

Climate room

PL (nonflowering GM plants)

Climate room

PC-I (flowering GM plants)

Greenhouse PKM-I (infected plants)

PKM-II (infected plants)

PKM-III (infected plants)

PKM-IV (infected plants)

Greenhouse PK-I, open soil (GM plants)

PK-II, floored (GM plants)

Containmentlevel 1 Level 1 containment is used when working with agents (Risk Group 1) that pose no risk to healthy adults:• Work may be carried out on an open bench top. • Street clothes and lab coats should not be kept together. • Disinfection should be carried out as required, using effective concentrations and contact times;

solutions should be replaced regularly. • Keep doors and windows closed

Containmentlevel 2  The following precautions, in addition to those for containment Level 1, are recommended:• Items should be autoclaved or chemically decontaminated before removal from the facility. • Use Class I or II biological safety cabinets for procedures that generate infectious particles. • Procedures should be carried out such that aerosol generation is minimized. • Lab coats should not be worn outside the lab. • Wear gloves to prevent skin contamination.

Containmentlevel 3Measures should include the recommendations outlined for levels 1 and 2, plus the following:• Lab personnel should be trained in handling, disposal, and emergency procedures. Written protocols

for these procedures should be developed and posted in a visible location. • Personnel should wear solid-front lab clothing, which should be autoclaved before laundering or

disposal.

Level 4  Not within Plant Sciences Group

Each level has its own rules

Break Please get yourself some coffee. Then watch this

16 minute video

Click for video……

Preparation of the experiment in the lab

• Every GMO or Pathogen project has a license, assigned to the responsible employee. Make sure you know what is in it!

• Pay special attention to the additional working rules in the license (they are aimed at the prevention of spreading)

• Let your responsible employee and the lab manager inform you

• Laminar air flow cabinet: filtered air is blown from the back to the front – Don’t work with

sporulating fungi in laminar air flow cabinet

• Down flow Biosafety cabinet: filtered air in and filtered air out– Working with toxins:

air outlet is outside

Working in flow cabinets

Pack in double bags with indicator tape and name

Autoclave within a week and for 30 minutes at 1210C

Dispose of immediately in container

(Make sure there are no hazardous substances in the waste (like phenol /chloroform)

Waste with GMO/ Pathogens

Transport Radix-Greenhouse• Prevent spreading

of reproductive parts during transport

• Double pack in a box with a lid or double plastic bags

• GM Plants only in absence of Agrobacterium tumefaciens

Labeling of GMO

GMO material should be clearly marked during storage, culture and transport with:

GMO-license number, your name, date, construct name

Experiments and storage of GMO’s should be registrated in GMO logbook of the Responsible Employee

Read rules on door & fill in the log

Working in the Greenhouse

Two final remarks on biosafety

• PSG has Biological Safety Officer. He can advise on GMO or Pathogen projects and is also auditing working methods and registrations.

• PSG is regularly audited by the government. PSG can loose one or more licenses if we don’t succeed to operate within the rules.

Thanks for your attention, please go to the survey