1. TRNG CAO NG C TR KHOA: CNG NGH SINH HC-MI TRNG NGNH:CNG NGH SINH HC LP: 08SH1

2. DANH SCH NHM V: 1. Nguyn Thnh Trung(1989) 2. Nguyn Thnh Trung(1988) 3. T Xun Truyn 4. Ng Anh Tun 5. H Thanh Tun 6. Nguyn Gic Vin 7. Bi Vit Yn

3. M U Tinh bt c bit n t hng nghn nm. Ngi La M gi l amilum, mt t bt ngun t ting Hy Lp, amilon. Tinh bt c vai tr c bit cung cp dinh dng cho con ngi. Tinh bt c tch ra t la m v ng cc. Bin tnh tinh bt l mt phn quan trng trong cng nghip sn xut bnh ko, bia

4. NI DUNG I. Cu to v tnh cht ca tinh bt III. ng dng ca tinh bt bin tnh. II. Bin tnh tinh bt bng enzim. IV. Kt lun

5. I. CU TO V TNH CHT CA TINH BT. 1. Cu to: Tinh bt l loi polysaccarit c khi lng phn t cao gm cc n v glucoz c ni nhau bi cc lin kt glycozit. (C6H10O5)n. Tinh bt bao gm 2 thnh phn: + Amiloza + Amilopectin 2. Hnh dng: C dng hnh trn, bu dc, hay a gic Tnh cht c l khc nhau nh nhit h ho, kh nng hp th xanh metylen ...

6. Amiloza Amilopectin Cu to Tnh cht -Gm 6 gc glucozo to thnh 1 vng xon c, lin kt 1- 4 Glicozit, to mch thng. - ha tan: + D ha tan - To mu it: + Cho mu xanh. + To bn - Gm lin kt 1- 4 Gluzit lin kt vi 1- 6 Glicozit to thnh chui xon c. + Kh ha tan(t0 cao). + Cho mu tm, . + To nht.

7. Cu to tinh bt

8. II. BIN TNH TINH BTII. BIN TNH TINH BT Trong thc t sn xut, ng vi mi mt sn phm thc phm thng i hi 1 dng tinh bt hoc mt dn sut tinh bt nht nh nh: tnh bt phi c ha tan tt, giu amiloza, amilopectin, tnh bn.v.v c c nhng loi hnh tinh bt ph hp ngi ta phi bin hnh tinh bt

9. Mc ch ca bin hnh tinh bt nhm: Ci bin cc tnh cht ca sn phm.Ci bin cc tnh cht ca sn phm. Tng gi tr cm quan.Tng gi tr cm quan.To mt hng mi.To mt hng mi.

10. Da vo bn cht ca phng php c th phn loi cc phng php nh sau: Phng php bin hnh vt lPhng php bin hnh vt l Phng php bin hnh bng enzym.Phng php bin hnh bng enzym. Phng php bin hnh ha hcPhng php bin hnh ha hc

11. 2.1. Bin hnh sinh hc tinh bt Cc tc nhn bin hnh tinh bt bng enzim: Cc enzym thy phn . Cc enzym xc tc phn ng tng hp cc oligosaccarit. Dextrin ha: s thy phn na vi v thu nhn maltodextrin.

12. 2.1.1.Tc nhn thy phn C ch thy phn bt u bng s lm yu lin kt glucozit C1-C4 phi thy phn. Khi lin kt glucozit b t s to nn mt ion oxicacbonium(trng thi chuyn). Oxicacbonium tc dng vi mt phn t nc v hai oligosaccarit c to thnh lin ri b tm hot ng ca enzym. Enzym a- amilaza thy phn lin kt a-1,4 trn nhiu mch v tn ti nhiu v tr ca cng mt mch, gii phng ra glucoz v cc oligosaccarit.

13. Kt qu tc ng ca a- amilaza thng lm gim nhanh nht ca dung dch tinh bt . Khi thy phn amiloza sn phm cui cng ch yu l maltoza v maltotrioza.Do maltotrioza bn hn nn vic thy phn n thnh maltoza v glucoz c thc hin ngay sau . C hai c ch tc dng ln amiloza trong dung dch: c ch tn cng nhiu ln v c ch tn cng u tin.

14. Thy phn enzym thu dextrin v cc sn phm c th Thy phn tinh bt bng enzim to ra ng, t ng c th to ra nhng sn phm khc nhau l c s chnh s dng tinh bt trong cng nghip . u im: + S phn ct tinh bt bi enzym c hiu hn . + To ra c nhng sn phm rt c th . + Phn ng nhanh, cng ngh n gin v t g b.

15. Enzim tham gia thy phn tinh bt

16. Gii thch quy trnh: Qu trnh to dung dch ng c th chia lm giai on: Dextrin ha ng ha ng phn ha

17. III. NG DNG BIN TNH BT TRONG SN XUT BIA 3.1. Nguyn liu: Nc. Men Hoa bia. Mch nha. Cht ph gia

18. Quy trnh sn xut bia

19. Quy trnh sn xut bia

20. Quy trnh gy mch nha

21. Bn ln men bia My nghin Bung nu bia Kho xut bia

22. Gii thch quy trnh sn xut bia: H2O: - Nc l nguyn liu c bn trong qu trnh sn xutNc l nguyn liu c bn trong qu trnh sn xut bia, nh hng rt ln n cht lng ca bia.bia, nh hng rt ln n cht lng ca bia. - Thnh phn ca nc ph thucThnh phn ca nc ph thuc vo lng nc cung cp. pH: - pH nh hng rt ln dn qu trnh sn xut bia. Cc ch tiu ha l:Cc ch tiu ha l: + m+ m + ha tan+ ha tan

23. Hoa HOUBLON:Hoa HOUBLON: L nguyn liu quan trng th hai trong sn xutL nguyn liu quan trng th hai trong sn xut bia.bia. u im: + To v ng v mi thm c trng cho bia. + Tit ra cc cht c kh nng tit trng. + Tng kh nng n nh cho bia.

24. Thnh phn ha hc ca Houblon: Cht ng = 18,5%Cht ng = 18,5% Tinh du = 0,5%Tinh du = 0,5% Polphenol = 3,5%Polphenol = 3,5% Protein = 10%Protein = 10% Cc cht v c = 8%Cc cht v c = 8% Cc dng hoa houlon: C 2 loi: + to hng (hm lng - axit cao). + to v ( hm lng - axit cao).

25. Nm men: Chng nm men.Chng nm men. - Chng nm men ln men chm Saccharomyces carlsbergencis. - y l loi nm men hiu kh dng trong qu trnh h hp v tng sinh khi ( 25- 300 C). Ngng hot ng nhit 400 C. - Nng dd ng 200% qu trnh pht trin nm men v ln men ngng hot ng. - Hm lng co2 v o2 nh hng ti kh nng tng sinh khi v tc pht trin nm men.

26. Quy trnh sn xut bia gm 3 giai on chnh: Ch bin dch ln menCh bin dch ln men Ln men chnh, ln men ph.Ln men chnh, ln men ph. Lc bia v chit bia.Lc bia v chit bia. Dy chuyn cng ngh trong khu nu: - Tip nhn tn tr nguyn liu go malt.Tip nhn tn tr nguyn liu go malt. - Nghin nguyn liuNghin nguyn liu - Trn bt nguyn liu vi ncTrn bt nguyn liu vi nc - ng hang ha - Lc dch ngLc dch ng - un si dch ng cng hoa houblon.- un si dch ng cng hoa houblon. - Lng cn v tch cn- Lng cn v tch cn - H nhit

27. Nu ng ha nguyn liu Mc ch: chuyn cc cht dng cao phn t c trong malt v dng d ha tan v to thnh cht chit (extract).extract). Qu trnh thy phn cc cht di tc dng ca cc enzim c trong malt. Cc cht d ha tan trong malt thng l ng v dextrin, cc cht v c v mt phn l protein. Cc cht kh ha tan phn ln l tinh bt, v tru v mt s hn hp cao phn t khc, khi nu xong s c d li trong b hm.

28. Phng php lcPhng php lc Lc bng thng lngLc bng thng lng Lc bng khung bnLc bng khung bn

29. B hm Bnh qun 100kg nguyn liu s c t 110 kg 120kg hm t 75- 80% nc. V trong b hm c cha nhiu cht c kh nng ln men, nu cha lu v nhit cao s pht trin VSV. Thnh phn ca b hm: - nc : 75- 80% - Cht bo : 2% - protein : 5% - Cellutose : 5% - protein digestible : 3,5% - Khong : 1%

30. Kim tra dch ng Dch ng l sn phm ca khu nu, nhng n l nguyn liu ca khu ln men, cho nn mun t mt qu trnh ln men tt, cht lng theo yu cu. Ngi ta cn nm vng cc ch tiu nh ng, pH, mn, mu, trong dch ng.v.vqua iu chnh li cng ngh ca mnh.

31. Quy trnh ln men ng thnh cn v co2 Trong qu trnh hot ng sng ca nm men, thng qua qu trnh TC v NL, tt c cc ng u c ln men chuyn ha thnh cn v co2. gii phng nng lng: PT: C6H12O6 2C2H5OH + 2 CO2 + Q y l qu trnh sinh ha phc tp, di tc dng ca Enzim amilzza v coenzim. Trong bia cha hn 60 loi ester nhng c 6 ester nh hng quyt nh n hng v ca bia.

32. 6 Enzim ng vai tr to hng v cho bia - Ethyl acetate- Ethyl acetate - phenylactate- phenylactate - Issoamylactate- Issoamylactate - Ethylcaproate- Ethylcaproate - IsobutylactateIsobutylactate - Ethycarylate- Ethycarylate Hm lng ester ph thuc vo tng loi bia t trng dch ca ng. Bo ha CO2 ph thuc vo nhit , nhit cng cao th ha tan ca CO2 cng km, v ngc li.

33. c tnh ca bia trc khi lc. Sau khi thi b nm men cn st li, lc men v cc cht gy huyn ph. Bia lc ny trng thi keo, n nh 0- 10 C, hm lng CO2 lc cha t trng thi bo ha phi t t nht 0,5%, t l men chit