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QIIME virtual machine download and installa;on
Alexander V. Alekseyenko
Download QIIME Virtual Machine
Click the download link
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Put the downloaded file on Desktop in directory QIIME
Click to unpack
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Wait for Archive U;lity to finish
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QIIME directory aKer unpacking
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Installing QIIME virtual machine
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Click New to add virtual machine
Click Con;nue
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Virtual Machine configura;on
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1. Type in name ‘QIIME’
2. Select ‘Linux’ 3. Select ‘Ubuntu (64 bit)’
4. Click
Selec;ng memory for the virtual machine
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Select at least 1024MB memory (select more if your machine has more physical
memory)
Click
Select QIIME virtual machine hard disk
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1. Put check mark
2. Select
3. Click icon
Locate unpacked QIIME image file
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1. Select image
Click ‘Open’
Select QIIME virtual machine hard disk
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Click ‘Con;nue’
QIIME virtual machine summary
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Click to finish
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QIIME virtual machine in VirtualBox
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Install VB Guest Addi;ons
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Click on Devices -‐> Install Guest Addi;ons…
Execute installa;on autorun
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Click to ok
Execute autorun
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Click to Run
Alterna;vely run from the command line
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When Guest Addi;ons installa;on is complete, Re-‐start the QIIME virtual machine
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Downloading reference sequences
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In terminal type: �wget hfp://greengenes.lbl.gov/Download/Sequence_Data/Fasta_data_files/core_set_aligned.fasta.imputed
Downloading reference sequences
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In terminal type: �wget hfp://greengenes.lbl.gov/Download/Sequence_Data/lanemask_in_1s_and_0s