Sarcoma
Learn more about the FusionPlex Sarcoma Panel at www.archerdx.com/sarcoma
FUSIONPlex
Archer™ FusionPlex™ Sarcoma Panel Genomic translocations represent an important aspect in sarcoma biology. Translocations have been reported in 20-30% of soft tissue cancers, with more than 30 distinct gene fusions recurring in specific sarcoma subtypes. Traditional methods of detecting transloca-tions, such as FISH, lack the high-resolution molecular characterization crucial for understanding the identity of these fusion partners. Amplicon-based enrichment methods (such as RT-PCR or traditional targeted sequencing) enable fusion identification, but require that fusion partners and breakpoints be known in advance. The FusionPlex Sarcoma Panel is a targeted sequencing assay that simultaneously detects and identifies fusions of 26 genes associated with soft tissue cancers. Using Archer’s proprietary Anchored Multiplex PCR™ based enrichment, fusions of all genes in this panel can be identified in a single sequencing assay, even without prior knowledge of fusion partners or breakpoints.
• Innovative – first comprehensive NGS-based test on the market
• Comprehensive – identify sarcoma- related fusions in a single assay
• Streamlined – reduce turn-around time and eliminate reflex testing
• Detailed – characterize all molecular fusion partners
For Research Use Only. Not for use in diagnostic procedures.
ALK FUS NTRK3 TCF12
CAMTA1 GLI1 PDGFB TFE3
CCNB3 HMGA2 PLAG1 TFG
CIC JAZF1 ROS1 USP6
EPC1 MEAF6 SS18 YWHAE
EWSR1 MKL2 STAT6
FOXO1 NCOA2 TAF15
Assay Targets Includes the following genes and their fusion partners:
Sarcoma Fusion Map
The FusionPlex Sarcoma Panel can be used to identify all of the genes list-ed in the diagram above. By targeting the genes highlighted in red, our Anchored Multiplex PCR enrichment chemistry has the ability to identify each of these sarcoma-associated fusion pairs.
PN-MKT-0016 REV A
WWTR1
YWHAE
EWSR1
FUS
SS18
COL1A1
JAZF1
TPM3
TPM4
ATICCAR
S
CLTCRANB
P2
SEC31A
CD74EZRGOPCLRIG3SDC4SLC34A2CDH11CNBPMYH9OMDTHRAP3AHRRHEY1SRFTEADTFE3NAB2LPPHM
GA2
ETV6
COL1A2
CICC11orf95
BCOR
ASPSCR1
ACTB
MEAF6EPCPAX7PA
X3
TAF15
TCF12
TFG
CAMTA1
FAM22A-B
CREB1
E1AFETV1FEVFLI1
NFATC2PBX1
POU5F1WT
1ZNF444ZSG
CREB3L1CR
EB3L2SSX1SSX2SSX4PLAG1JJAZ1
YAP1STAT6NTRK3DUX4MKL2CCNB3GLI1
ATF1
DD1T3
ERG
PDGFB
FKHR
PHF1
NR4A3
NCOA2USP6
ALK
ROS1 W
WTR1
YWHAE
EWSR1
FUS
SS18
COL1A1
JAZF1
TPM3
TPM4
ATICCAR
S
CLTCRANB
P2
SEC31A
CD74EZRGOPCLRIG3SDC4SLC34A2CDH11CNBPMYH9OMDTHRAP3AHRRHEY1SRFTEADTFE3NAB2LPPHM
GA2
ETV6
COL1A2
CICC11orf95
BCOR
ASPSCR1
ACTB
MEAF6EPCPAX7PA
X3
TAF15
TCF12
TFG
CAMTA1
FAM22A-B
CREB1
E1AFETV1FEVFLI1
NFATC2PBX1
POU5F1WT
1ZNF444ZSG
CREB3L1CR
EB3L2SSX1SSX2SSX4PLAG1JJAZ1
YAP1STAT6NTRK3DUX4MKL2CCNB3GLI1
ATF1
DD1T3
ERG
PDGFB
FKHR
PHF1
NR4A3
NCOA2USP6
ALK
ROS1
5’ Partner3’ Partner
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