Sarcoma

Learn more about the FusionPlex Sarcoma Panel at www.archerdx.com/sarcoma

FUSIONPlex

Archer™ FusionPlex™ Sarcoma Panel Genomic translocations represent an important aspect in sarcoma biology. Translocations have been reported in 20-30% of soft tissue cancers, with more than 30 distinct gene fusions recurring in specific sarcoma subtypes. Traditional methods of detecting transloca-tions, such as FISH, lack the high-resolution molecular characterization crucial for understanding the identity of these fusion partners. Amplicon-based enrichment methods (such as RT-PCR or traditional targeted sequencing) enable fusion identification, but require that fusion partners and breakpoints be known in advance. The FusionPlex Sarcoma Panel is a targeted sequencing assay that simultaneously detects and identifies fusions of 26 genes associated with soft tissue cancers. Using Archer’s proprietary Anchored Multiplex PCR™ based enrichment, fusions of all genes in this panel can be identified in a single sequencing assay, even without prior knowledge of fusion partners or breakpoints.

• Innovative – first comprehensive NGS-based test on the market

• Comprehensive – identify sarcoma- related fusions in a single assay

• Streamlined – reduce turn-around time and eliminate reflex testing

• Detailed – characterize all molecular fusion partners

For Research Use Only. Not for use in diagnostic procedures.

ALK FUS NTRK3 TCF12

CAMTA1 GLI1 PDGFB TFE3

CCNB3 HMGA2 PLAG1 TFG

CIC JAZF1 ROS1 USP6

EPC1 MEAF6 SS18 YWHAE

EWSR1 MKL2 STAT6

FOXO1 NCOA2 TAF15

Assay Targets Includes the following genes and their fusion partners:

Sarcoma Fusion Map

The FusionPlex Sarcoma Panel can be used to identify all of the genes list-ed in the diagram above. By targeting the genes highlighted in red, our Anchored Multiplex PCR enrichment chemistry has the ability to identify each of these sarcoma-associated fusion pairs.

PN-MKT-0016 REV A

WWTR1

YWHAE

EWSR1

FUS

SS18

COL1A1

JAZF1

TPM3

TPM4

ATICCAR

S

CLTCRANB

P2

SEC31A

CD74EZRGOPCLRIG3SDC4SLC34A2CDH11CNBPMYH9OMDTHRAP3AHRRHEY1SRFTEADTFE3NAB2LPPHM

GA2

ETV6

COL1A2

CICC11orf95

BCOR

ASPSCR1

ACTB

MEAF6EPCPAX7PA

X3

TAF15

TCF12

TFG

CAMTA1

FAM22A-B

CREB1

E1AFETV1FEVFLI1

NFATC2PBX1

POU5F1WT

1ZNF444ZSG

CREB3L1CR

EB3L2SSX1SSX2SSX4PLAG1JJAZ1

YAP1STAT6NTRK3DUX4MKL2CCNB3GLI1

ATF1

DD1T3

ERG

PDGFB

FKHR

PHF1

NR4A3

NCOA2USP6

ALK

ROS1 W

WTR1

YWHAE

EWSR1

FUS

SS18

COL1A1

JAZF1

TPM3

TPM4

ATICCAR

S

CLTCRANB

P2

SEC31A

CD74EZRGOPCLRIG3SDC4SLC34A2CDH11CNBPMYH9OMDTHRAP3AHRRHEY1SRFTEADTFE3NAB2LPPHM

GA2

ETV6

COL1A2

CICC11orf95

BCOR

ASPSCR1

ACTB

MEAF6EPCPAX7PA

X3

TAF15

TCF12

TFG

CAMTA1

FAM22A-B

CREB1

E1AFETV1FEVFLI1

NFATC2PBX1

POU5F1WT

1ZNF444ZSG

CREB3L1CR

EB3L2SSX1SSX2SSX4PLAG1JJAZ1

YAP1STAT6NTRK3DUX4MKL2CCNB3GLI1

ATF1

DD1T3

ERG

PDGFB

FKHR

PHF1

NR4A3

NCOA2USP6

ALK

ROS1

5’ Partner3’ Partner