332 PATENT ABSTRACTS

practice, a site-deactivating medium such as an animal- or vegetable-derived total biological fluid or extract (e.g., plasma or gelatin) is cova- lently bound to the vessel wall surfaces for deac- tivation purposes. In preferred forms the process is solid-state, wherein one of the components of the antigen-antibody reaction is coupled cova- lently to the coating medium, and a reaction mix- ture fraction including the sample being determined and the other component which has been labeled, for instance by means of a colorimetfically active enzyme.

4414323

M E T H O D F O R M E A S U R I N G T R A C E E N Z Y M E

Nobuhito Masuda, Minami ashigara, Japan as- signed to Fuji Photo Film Co Ltd

In a photochemical measurement methodof a trace enzyme which comprises: using a synthetic substrate bringing either the reaction product formed by enzyme reaction or the unreacted syn- thetic substrate into contact with silver halide, developing the same, and measuring optical density of the formed silver image and/or colored dye, the synthetic substrate comprises at least one structure (A) which is specifically con- tacted with an enzyme to be measured and at least one photographically fogging agent struc- ture (B) in the molecule thereof.

4 4 1 2 ~ 1

I S O L A T I O N O F B A C T E R I A L L U C I F E R A S E

Thomas O Baldwin, Thomas F Holzman as- signed to Board of Trustees of The University of Illinois

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1112

The present invention provides a novel means for the isolation of bacterial luciferase and a novel affinity resin useful in said isolation.

4411999

I M M O B I L I Z A T I O N O F E N Z Y M E S O N G R A N U L A R G E L A T I N

Shigeki Shigesada, Hironoshi Kitagawa, Toshio Mihara, Yoshiaki lshimatsu, Machida, Japan assigned to Denki Kagaku Kogyo Kabushiki Kaisha

A process for producing an immobilized enzyme composition comprises simultaneously reacting a non-proteolytic enzyme and a water soluble- multifunctional reagent with a non-hardened granular gelatin in an aqueous medium wherein the reacting is carried out in the presence of a water-soluble protein polymer compound in an amount of from 0.01 to 2 parts by weight relative to one part of the non-proteolytic enzyme. The non-proteolytic enzyme forms a uniform film on the surface of the granular gelatin and the bond between the non-proteolytic enzyme and the granular gelatin is strengthened by the water soluble protein polymer.

4 4 1 1 ~ 9

P R O C E S S E S A N D D E V I C E S F O R D E T E C T I O N O F S U B S T A N C E S

S U C H A S E N Z Y M E I N H I B I T O R S

Ann E Grow assigned to Midwest Research In- stitute

The disclosed methods and devices for detecting or monitoring or identifying substances (such as chemical warfare agents) and residual environ- mental pollutants (such as pesticides) utilize the discovery that spectra (e.g. infrared absorption spectra) of an uninhibited enzyme (e.g., a cholinesterase) can differ from spectra of the same enzyme which has been complexed with the agent pollutant. For example, the infrared spectrum of uninhibited butyrylcholinesterase (BuChE) lacks a distinct absorption peak found