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Where is the mutation?
D. mau.
D. sech.xxxxxx
x
xx
x
x
Highresolution mapping of the cisregulatory mutation
D. sechellia
x
svb
selection of recombinants based on eye color
D. mauritiana
svbP[w+] P[w+]
1.2 Mb
recombination mapping
50 kb
reporter
enhancer bashing
AlistairMcGregor
Among progeny individuals: select [orange eye] flies:
3/100 flies
Highresolution mapping of the cisregulatory mutation
D. sechellia
x
D. mauritiana
svbP[w+] P[w+] svb
selection of recombinants based on eye color
1.2 Mb
50 kb
reporter
enhancer bashing
recombination mapping
One recombination event
every 2kb
D. mauritianalike
D. sechellialike
D. sechellia
D. mauritiana
D. mauritianalike
D. sechellialike
D. sechellia
D. mauritiana
Five types of recombinants
Three new phenotypes
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QuickTime et unᆰd compresseur TIFF (non compress )ホ ホ
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QuickTime et unᆰd compresseur TIFF (non compress )ホ ホ
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QuickTime et unᆰd compresseur TIFF (non compress )ホ ホ
sont requis pour visionner cette image.
Three enhancer regions
50 kb
reporter
D. melanogaster
reporter
D. sechellia
AlistairMcGregor
Three enhancer regions
50 kb
reporter
D. sechellia
reporter
D. melanogaster
AlistairMcGregor
D. sechelliax
D. mauritiana
svbP[w+] P[w+] svb
selection of 600 recombinants based on eye color
1.2 Mb
Where are the mutations responsible?
1
high recombination
Among progeny individuals: select [orange eye] flies:
3/100 flies
D. sechelliax
D. mauritiana
svbP[w+] P[w+] svb
selection of 600 recombinants based on eye color
1.2 Mb
What are the mutations responsible?
1
high recombination selection of 50 recombinants based on PCR markers
PCR marker
PCR marker
phenotyping
100 kb
2
3
svb
One recombination event
every 2kb
D. sechelliax
D. mauritiana
svbP[w+] P[w+] svb
selection of 600 recombinants based on eye color
selection of 50 recombinants based on PCR markers
PCR marker
PCR marker
phenotyping
1.2 Mb
100 kb
What are the mutations responsible?
1
2
3
svb
D. mauritianalike
D. sechellialike
D. sechellia
D. mauritiana
D. mauritianalike
D. sechellialike
Five types of recombinants
Three new phenotypes
D. sechellia
D. mauritiana