What is Bio-technology?What is Bio-technology?Why is it important to understand Why is it important to understand

your genetic make up?your genetic make up?How does this information affect How does this information affect

your life? Society?your life? Society?

http://www.komonews.com/news/http://www.komonews.com/news/consumer/17071161.htmlconsumer/17071161.html

Advancement of technology for research associated with life science. Especially Genetic Engineering (changing genes and recombinant DNA (changing DNA sequences)

Stolen IPOD? Cell Phone?Stolen IPOD? Cell Phone?

We are going to begin EHS CSI training!We are going to begin EHS CSI training!

Eliminating crime at EHS by Eliminating crime at EHS by incorporating electrophoresis into our incorporating electrophoresis into our discipline policy. Would you like to discipline policy. Would you like to

help?help?

Tools you need to learn to use…Tools you need to learn to use…Micro pipettes… Micro pipettes… Electrophoresis…Electrophoresis… Before we can use these tools we will need Before we can use these tools we will need

training on how they work…training on how they work…

What is the metric system?What is the metric system?..

How big is a Liter?How big is a Liter? How big is a mili Liter?How big is a mili Liter? mL(1/1000 L) mili means one thousanthmL(1/1000 L) mili means one thousanth How big is a micro liter compared to a L and a How big is a micro liter compared to a L and a

mL?mL? (1/1000 of a mL) (1/1,000,000 of a L)(1/1000 of a mL) (1/1,000,000 of a L) Which of these units do you think would be the Which of these units do you think would be the

best for looking at pieces of DNA?best for looking at pieces of DNA? Why?Why?

Standard measurement system Standard measurement system used through out the worldused through out the world

The MICRO LITER!!The MICRO LITER!!

DNA IS SO SMALL THAT IT NEEDS TO DNA IS SO SMALL THAT IT NEEDS TO BE LOOKED AT IN VERY TINY PIECESBE LOOKED AT IN VERY TINY PIECES

What tool should we use to measure DNA?What tool should we use to measure DNA? Micro-pipettes allow us to take samples as Micro-pipettes allow us to take samples as

small as .5 small as .5 µL and as large as 1000 µL (1 µL and as large as 1000 µL (1 mL)mL)

Are you a metric system master?Are you a metric system master?

When I convert from L to mL will the number get When I convert from L to mL will the number get smaller or larger? smaller or larger?

Larger, I will move my decimal place 3 spaces to Larger, I will move my decimal place 3 spaces to the right because it is a thousand times smaller.the right because it is a thousand times smaller.

When I convert from mL to When I convert from mL to µL will the number µL will the number get smaller or larger?get smaller or larger?

Larger, I will move my decimal place 3 places to Larger, I will move my decimal place 3 places to the right.the right.

Metric System Master?Metric System Master? When I convert from When I convert from µµL to mL will the number L to mL will the number

get smaller or larger?get smaller or larger? Smaller, I will move my decimal place 3 places to Smaller, I will move my decimal place 3 places to

the left. For example 2 the left. For example 2 µµL = .002 mLL = .002 mL

When I convert from mL to L will the number get When I convert from mL to L will the number get smaller or larger?smaller or larger?

Smaller. I will move my decimal place 3 spaces to Smaller. I will move my decimal place 3 spaces to the left.the left.

2.0 mL = .002 L 2.0 mL = .002 L

Factor Label MethodFactor Label Method

A great way to set up conversionsA great way to set up conversions Start the conversion with what you know and Start the conversion with what you know and

make a relationship that relates to what you want make a relationship that relates to what you want to knowto know

For example when determining how many For example when determining how many µL are in 20 L set up this conversionµL are in 20 L set up this conversion

20L 20L 1,000,000 µL 1,000,000 µL 1 L1 LThis means 20 X 1,000,000 =20,000,000 This means 20 X 1,000,000 =20,000,000 µLµL

Metric System Master???Metric System Master???

If I have L and want If I have L and want µL what should I do?µL what should I do? Move the decimal place 6 places to the rightMove the decimal place 6 places to the right Or set a conversion using factor label methodOr set a conversion using factor label method

I will give you a measurement and you must tell me how I will give you a measurement and you must tell me how many many µµL I have… Use Factor Label methodL I have… Use Factor Label method

2 2 µL = how many L?µL = how many L? 2 µL 2 µL 1 L1 L

1,000,000 µL1,000,000 µL2µL X 1L /1,000,000µL2µL X 1L /1,000,000µL==

.000002L.000002L 87 mL = how many µL?87 mL = how many µL?

87mL 87mL 1,000 µL1,000 µL 1 mL1 mL87mLx 1000 µL/ 1mL87mLx 1000 µL/ 1mL

87,000 µL87,000 µL

Try these on your own….Try these on your own…. 100 100 µL = How many LµL = How many L 2,300,000 L = How many mL2,300,000 L = How many mL 45 L = How many µL?45 L = How many µL?

1. .0001

2. 2,300,000,000mL

3. 45,000,000 µL

EQUIPMENT!!!EQUIPMENT!!!

You must show competentence before you You must show competentence before you can join the EHS crime fighting team!!can join the EHS crime fighting team!!

P-10P-10 range of .5-10 range of .5-10 µLµL P-20 range of 2- 20 µLP-20 range of 2- 20 µL P-200P-200 range of 20-200 µLrange of 20-200 µL P 1000P 1000 range of 200-1000 µLrange of 200-1000 µL

IF YOU USE THE WRONG PIPETTE IT IF YOU USE THE WRONG PIPETTE IT WILL MAKE THEM LESS ACCURATE WILL MAKE THEM LESS ACCURATE

AND MAY DESTROY THEM..AND THEY AND MAY DESTROY THEM..AND THEY MIGHT EXPLODE!MIGHT EXPLODE!

If I want to measure 2 If I want to measure 2 µL which pipette would I µL which pipette would I choose?choose?

P-10P-10 If I want to measure 178 µL which pipette would If I want to measure 178 µL which pipette would

I choose?I choose? P-200P-200 If I want to measure 778 µL which pipette would If I want to measure 778 µL which pipette would

I choose?I choose?

Try these on your own.Try these on your own.

Tell me the pipet for the specific Tell me the pipet for the specific measurement.measurement.

600 600 µLµL 25 µL25 µL 225 µL225 µL 90 µL90 µL 1 µL 18 µL1 µL 18 µL

1. P-1000 4. p-200

2. P-1000 5. P-200

3. P-10 6. P-20

Use Factor label method to Use Factor label method to determinedetermine

How many mL are in 2,000 How many mL are in 2,000 ччL?L?

How many How many ччL are in 3 L?L are in 3 L?

How to use pipetsHow to use pipets

Lets look at our Fabulous handout your Lets look at our Fabulous handout your wonderful teacher gave you….wonderful teacher gave you….

Tommorrow, I’ll be back to…Tommorrow, I’ll be back to… Make sure you are measuring the correct Make sure you are measuring the correct

amounts of DNA?amounts of DNA? In order to participate in the lab and gain In order to participate in the lab and gain

your CSI Terrace crime fighting skills…your CSI Terrace crime fighting skills… Finish Metric system/Pipet worksheetFinish Metric system/Pipet worksheet We’ll practice using pipets tommorrowWe’ll practice using pipets tommorrow

We will continue our CSI EHS crime We will continue our CSI EHS crime fighter training tommorrow…fighter training tommorrow…

Practice your conversions and pipette Practice your conversions and pipette choice worksheet tonight!choice worksheet tonight!