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CURRICULUM FRAMEWORK AND SYLLABUSFOR OUTCOME BASED EDUCATION IN

Master of Science in Biotechnology Degree Program

FOR THE STUDENTS ADMITTED FROM THEACADEMIC YEAR 2019 - 2020 ONWARDS

HICAS

HINDUSTHAN COLLEGE OF ARTS AND SCIENCE (AUTONOMOUS)(Affiliated to Bharathiar University and Accredited by NAAC)

COIMBATORE-641028TAMILNADU, INDIA.

Phone: 0422-4440555 Website: www.hindusthan.net/hicas/

http://www.hindusthan.net/hicas

HINDUSTHAN COLLEGE OF ARTS AND SCIENCEDEPARTMENT OF BIOTECHNOLOGY

VISION

To provide world class education to the students to face global challenges and to inculcate the latest

trends in technological advancement. To cater the needs of the environmental and ethical values in the

mind of students to become good citizens and entrepreneurs.

MISSION

The Mission of the College is to pursue a philosophy of perceptual acquisition of knowledge.

The important policy is to provide value based education and to bring out the hidden potentials in

students that equip them to approach life with optimism.

PROGRAMME EDUCATIONAL OBJECTIVES (PEO)

Post Graduates of Biotechnology program will be,

PEO1: Understand and apply the fundamental knowledge of biological sciences, concepts of

Biotechnology and related aspects of Science and Technology.

PEO2: Apply the acquired practical skills in research and development of process and

product for Human Welfare.

PEO3: To produce Biotechnologists who will be employable in core Biotech/ Pharma

industries, domain based software services, academic/ Industry sponsored research/

Entrepreneurs.

PEO4: Demonstrate professional and ethical attitude with awareness of major issues at the

forefront of the discipline and think about the social entailment of their work, especially its

impact on safety, health and environment for sustainable development.

PEO5: Exhibit strong, independent learning, analytical, problem solving skills, and ability to

work in teams and provide opportunity to manage and work on multidisciplinary projects.

PROGRAM OUTCOME (PO) :

PO1: Key principles of biochemical functioning at an advanced level, knowledge of

contemporary issues and being inquisitive in understanding cutting edge areas of

Biotechnology, its Industrial applications.

PO2: Will possess an in-depth understanding in the areas of biotechnology chosen for

research emphasis on societal context.

PO3: Ability to design a system, component, or process to meet desired needs within

realistic constraints such as economic, environmental, social, political, ethical, health and

safety, manufacturability, and sustainability.

PO4: Understanding professional, ethical responsibility and to articulate debate, analyze

scientific problems and to think critically to analyze results with clarity.

PROGRAM SPECIFIC OUTCOME (PSO) :

PSO1: To make students competent in the field of biotechnology and its allied areas. To

Acquire knowledge in domain of biotechnology enabling their applications in industry and

research/ an effective entrepreneur to develop biotechnology start-ups.

PSO2: To Recognize the importance of Bioethics, IPR, entrepreneurship, Communication

and management skills so as to guide next generation of industrialists.

PSO3: To make the students, an eminent researcher or Scientist in the field of biotechnology

to discover/ innovate unique products for societal need with proper ethical statute.

HINDUSTHAN COLLEGE OF ARTS AND SCIENCE (AUTONOMOUS)COIMBATORE – 641 028

M.Sc., BIOTECHNOLOGYSCHEME OF EXAMINATIONS – CBCS PATTERN

(For the Students Admitted from the Academic Year 2019 -2020 and Onwards)

CODE NO. COURSE TYPE

SUBJECT

LE

CT

UR

E

HR

S/

WE

EK

EX

AM

D

UR

AT

ION

(H

RS)

MAX. MARKSCREDIT POINTSIE EE

TOTAL

First Semester

19BTP01 DSC Cell and Molecular Biology 4 3 30 70 100 419BTP02 DSC Applied Microbiology 5 3 30 70 100 419BTP03 DSC Biochemistry 5 3 30 70 100 4

19BTP04A DSEElective I Plant and Animal System Physiology

4 3 30 70 100 319BTP04B

Elective I Occupational Health and Industrial Safety

19BTP05 SECPractical I - Cell and Molecular Biology

4 6 40 60 100 3

19BTP06 SEC Practical II - Microbiology 4 6 40 60 100 319BTP07 SEC Practical III – Biochemistry 4 6 40 60 100 3

Credits 24Second Semester

19BTP09 DSC Genetic Engineering 5 3 30 70 100 4

19BTP10 DSC Research Methodology and Biostatistics

5 3 30 70 100 4

19BTP11 DSC Bioprocess Technology 5 3 30 70 100 4

19BTP12A DSE Elective II Bioethics, Biosafety and IPR 5 3 30 70 100 3

19BTP12B Elective II Genomics and Proteomics

19BTP13 SEC Practical IV- Genetic Engineering 4 6 40 60 100 319BTP14 SEC Practical V - Bioprocess Technology 4 6 40 60 100 3

19BTP15 AECC Self Supportive Course:Biodiversity and Conservation Biology - 3 30 70 100 3

16GSP01 AEE Skill Based - Cyber Security 2 - 100 - 100 2Credits 26

Students should complete Online course, Mini project, News review/Poster Presentation at the End of the First Year

Third Semester

19BTP16 DSC Immunology and Immunotechnology 5 3 30 70 100 4

19BTP17 DSC Animal and Pharmaceutical Biotechnology

5 3 30 70 100 4

19BTP18 DSC Plant Biotechnology 4 3 30 70 100 4

19BTP19A DSEElective III Developmental Biology and Behavioral Studies 4 3 30 70 100 3

19BTP19BElective III Biotechniques

19BTP20 SEC Institutional Training - - 100 - 100 3

19BTP21 SECPractical VI - Immunology and Immunotechnology

4 6 40 60 100 3

19BTP22 SECPractical VII - Animal and Pharmaceutical Biotechnology

4 6 40 60 100 3

19BTP23 SEC Practical VIII - Plant Biotechnology 4 6 40 60 100 3 Credits 27

Fourth Semester19BTP24 DSC Environmental Biotechnology 5 3 30 70 100 4

19BTP25 SEC Practical IX - Environmental Biotechnology

5 6 40 60 100 3

19BTP26 SEC Project Work 1 - 50 150 200 6

Credits 13Students should complete Online course, Case study Analysis/Research Article Review/Paper Presentation at the End of the Year

90

No of papers Course Type

Total Credit Points

1Ability Enhancement Compulsory course(AECC) 03

1 Ability Enhancement Elective (AEE) 02

10 Discipline Specific course(DSC) 40

3 Discipline Specific Elective(DSE) 09

11 Skill Enhancement Course(SEC) 36

26 TOTAL 90

PG-REGULATIONS(From 2019-2020 and Onwards)

1. Internal Marks for all PG

Components MarksTest I 5

Model Exam 10Assignment 5Attendance* 5

Seminar 5(3+2)**

TOTAL 30

*Split-up of Attendance Marks for PG

75-79 - 1 marks 80-84 - 2 marks 85-89 - 3 marks 90-94 - 4 marks 95-100 - 5 marks

**3-For External paper presentation/ Mini Project

**2-Internal paper presentation/ Mini Project

Question Paper Pattern for IE test I

Duration: Two Hours Maximum: 50 Marks

Section-A (3 x 6=18 Marks)

Answer ALL Questions Either or Type

ALL questions carry EQUAL Marks

Section-B (4 x 8=32 Marks)

Answer ALL Questions

Either or Type

ALL questions carry EQUAL Marks

Question Paper Pattern for IE Model Exam

Duration: Three Hours Maximum: 70 Marks

SECTION – A (5x6=30 marks)Answer ALL Questions

ALL Questions carry EQUAL Marks

Q.No 1 to 5: Either or type questions(One question from each Unit)

SECTION – B (5x8=40 Marks)Answer ALL Questions



2 a) Components for Practical I. E.

Components Marks

Test –I 20

Test - II 20

Total 40

2 b) Components for Practical E. E.

Components Marks

Completion of Experiments 50

Record 5

Viva 5

Total 60

3. Institutional/ Industrial Training, Mini and Major Project Work

Institutional / Industrial Training MiniProject

Project Work

Components Marks Components Marks

I.EWork Diary Report Viva –voceExamination

255025

- 5050

I. Ea) Attendance Marks 20b) Review Marks 30 50

Total 100 100

E.E*1

a) Final Report Marks 120b) Viva-voce Marks 30 150

Total 200

*1Evaluation of report and conduct of viva voce will be done jointly by Internal and External

Examiners.

4. Components for Cyber Security Paper

Components Marks

Two Tests (2 x 40) 80

Two assignments (2 x 10) 20

Total-------100

=====

The question paper pattern is as follows:

a) Test I – 2 hours [4 out of 7 essay type questions] 4 x 10 = 40Marksb) Test II – 2 hours [4 out of 7 essay type questions] 4 x 10 = 40 Marks

------------------------Total = 80 Marks------------------------

The passing minimum for Cyber Security is 50 In case the candidate fails to secure 50 marks which is the passing

minimum, he/she may have to reappear for the same in the subsequent semesters.

BOS meeting approved: Approved in 5th Academic Council meeting on :

5. Question Paper Pattern for EE Theory

Duration: Three Hours Maximum: 70 Marks

SECTION – A (5x6=30 marks)Answer ALL Questions



SECTION – B (5x8=40 Marks)Answer ALL Questions




MASTER OF SCIENCEProgramme Code: DSC Programme Title: Master of Science in Biotechnology

Course Code: 19BTP01 Course Title Batch: 2019-2021CELL AND MOLECULAR BIOLOGY Semester: I

Hrs/Week: 4 Credits: 4

Course ObjectiveTo impart knowledge on various aspects of Cell and Molecular Biology which

Supports Students to have Solid Foundation in Basic Understanding.

Course Outcomes (CO)

K1 CO1 Understand the knowledge of cell structures, functions, cellular organelles and various metabolic processes will help students to design experiments with appropriate understanding and leads to conduct genuine research.

K2 CO2 Describe and discuss the properties and biological significance of the major classes of molecules found in living organisms and the relationship between molecular structure and biological function.

K3 CO3 Represent and illustrate the structural organization of genes and the control of gene expression ,replication and photosynthesis.

K4 CO4 Outline the processes that control eukaryotic cell cycle and cell death and link the rapid advances in cell and molecular biology to a better understanding of diseases, including canc

Mapping of Outcomes

POCO PO1 PO2 PO3 PO4

CO1 S S S S

CO2 S M L L

CO3 S S M L

CO4 S S M L

S - Strong; M-Medium; L-Low.


CELL AND MOLECULAR BIOLOGY

Unit No. Topics Hours

I

Introduction to Different Types of CellsTypes of Cells - Prokaryotic and Eukaryotic Cell Structure and Intracellular Organelles – Nucleus, Mitochondria, Chloroplast, Ribosomes, golgi complex, Endoplasmc reticulum, Peroxysomes and Microbodies; Fractionation and Purification of Subcellular Organelles.

9

II

Cell Division, Cell Cycle and CytoskeletonEvents of Mitosis and Meiosis. Cell Cylce and its regulation. Cytoskeleton - Cell Motility and Cellular Movements – Microtubules – Microfilament Specialized Cell Structure and Function (Muscle Cell, Nerve Cell and Motile Cell).

9

III Cell Signaling Structure and Functions of Plasma Membrane – Cell Recognition, Cell to Cell Signaling – Receptor and Receptor – Response Mechanisms G protein coupled receptors – Jak/STAT Pathway, Receptor Tyrosine Kinase pathways

11

IV

Central Dogma of CellDNA Replication, RNA Transcription and Processing, Interaction of mRNA, rRNA and tRNA on Protein Synthesis. Mitochondrial Electron Transport System – Oxidative Reactions in Microbodies– Pathways of Photosynthesis – Light and Dark Reactions.

10

VGene transfer, Gene Regulation, Genetic recombination DNA as a genetic material – Conjugation, Transformation and Transduction. Mutation – Spontaneous and Induced; Operon concept – lac, trp and ara operons Recombination – Holliday model;

9

Text Book:1. Darnell Lodish, “Molecular Cell Biology”, Baltimore Scientific Books, USA.2. Ajoy Paul”Cell and Molecular Biology,Books and Allied

Reference Books:1. Alberts Bruce, “Molecular Biology of Cell”, Garland Science, New York. 2. Cooper G.M, “The Cell: A Molecular Approach”, ASM Press, Ohio. 3.Lewin” Genes VII” Pearson Prentice Hall4.Susan viseli”Cell and Molecular Biology, Lippincott Williams & Wilkins,5.Karps”Cellbiology” John Wiley

Course Designed by Verified by HOD Checked by Approved by

Dr.S.KOMATHI


Programme Code: DSC Programme Title: Master of Science in Biotechnology

Course Code: 19BTP02 Course Title Batch: 2019-2021APPLIED MICROBIOLOGY Semester: I


Course Objective1. To provide knowledge on the Fundamentals of Microbiology and to help the students to understand the different fields of Microbiology. 2. To know about the theoretical basis of the tools, technologies and methods common to microbiology.Course Outcomes (CO)

K1 CO1 Gain knowledge on importance of microbial taxonomy and systematics

K2 CO2 Discuss various techniques involved in Molecular taxonomy and Phylogeny

K3 CO3 Understand various modern diversity study methods and get idea on Anaerobic microbiology

K4 CO4 Apply the appropriate tools in Medicine and Human health.

Mapping of Outcomes


CO1 S S S S

CO2S S M L

CO3 S M L M

CO4 S S L M



APPLIED MICROBIOLOGYUnit No. Topics Hours

I

Introduction and TechniquesHistory & scope of Microbiology, Microscopic techniques: Principles, working mechanism and application-simple, compound, dark field and phase contrast microscope, fluorescence, SEM & TEM.

12

II

Culture Techniques and SterilisationMicrobial cultures: Methods of pure culture technique- Serial Dilution, Pour Plate, Spread Plate and Streaking methods. Microbiological Media- Types and composition of media. Staining Techniques: Simple, Differential (Gram’s AFB), special- capsular staining (negative), spore, Acid Fast Staining, Fungal Staining - LPCB.Methods of sterilization: physical methods-Dry heat, moist heat, radiation methods, filtration methods, chemical methods & their application. Preservation and maintenance of Microbial cultures- Lyophilizers, Deep freezer

12

III

Microbial TaxonomyDomains and Kingdoms of Life- Bacterial Nomenclature- Classification of Bacteria by Physiological, Metabolic, Serological and Molecular methods- Bergey’s Manual of Systematic Bacteriology with general characteristics of each division- Numerical Taxonomy- 16S rRNA based classification. Archeabacterium, Actinomycetes- Structure and Classification.

12

IV

Food and Industrial MicrobiologyNormal Microflora in Milk, Meat, Canned Food and Stored Grains. Milk Quality Tests - Chemical Preservations, Radiation. Fermented Food: Cheese – Idli – Yogurt. Food Borne Diseases: Botulism – Salmonellosis – Shigellosis. Microbial Production of Organic Acids - Citric Acid; Antibiotics – Penicillin and Streptomycin; Enzymes - Amylase and Lipase; Vitamin - B12; Alcoholic beverage – Beer and wine

12

V

Medical Microbiology: Pathogenicity and laboratory diagnosis of Corynebacterium diphteriae, Streptococccus pyogenes E.coli Salmonella sp, Pathogenicity and Laboratory diagnosis of viral infections – Hepatitis, Dengue virus, HIV and Ebola virus. Pathogenicity and laboratory diagnosis of Entamoeba histolytica, Taenia solium, Plasmodium vivax, Wucheraria bancrofti Mycosis –Superficial, Subcutaneous and Systemic infections – Cryptococcosis, Candida albicans.

12

Text Book:1.Presscott L.M, Harley J.P and Klein D.A, “Microbiology”, Tata Mc Graw Hill, New Delhi. Reference Books:1.Frazier W.C and West Hoff D.C, “Food Microbiology”, Tata McGraw Hill Ltd, New Delhi.2.Stanbury P.F, Whittaker A and Hall S.J, “Principles of Fermentation Technology”, Butterworth-Heinmenn Publishers, New York. 3.Introduction to Plant Biotechnology by H.S. Chawla OladeleOgunseitan. Microbial Diversity - Form and Function in Prokaryotes. 4. Wolfgang R. Streit and Rolf Daniel. Metagenomics: Methods and Protocols. 5. A. Mark Osborn and Cindy J. Smith. Molecular Microbial Ecology.Taylor and Francis G


Dr.V.VIJAYALAKSHMI



Course Code: 19BTP03 Course Title Batch: 2019-2021BIOCHEMISTRY Semester: I


Course Objective

To develop understanding and provide scientific basics of the life processes at the

molecular level and to explain the structure, function and inter-relationships of biomolecules.


K1 CO1 Acquire knowledge on the building blocks of the macromolecules, their chemicalproperties and their modification and their importance in normal functioning of living organisms.

K2 CO2 To develop a thorough Understanding about the various biochemical reactions- thermodynamics - energy production through biochemical process responsible for the manifestation of life disease and metabolic errors.

K3 CO3 Understand the metabolic pathways and identify how the genetic abnormalities disturb the normal homeostasis and link with pathological conditions

K4 CO4 To develop the various applications of biochemistry in medicine, agriculture, and pharmaceuticals

Mapping of Outcomes


CO1 S L M L

CO2 S S S L

CO3 M S M S

CO4 S S S S



BIOCHEMISTRYUnit No. Topics Hours

I

Atoms, Molecules and Chemical BondsClasses of Organic Compounds and Functional Groups. Covalent and Non-covalent Interactions - Vander Waal’s – Electrostatic – Hydrogen Bonding and Hydrophobic Interactions; Respiration and Photosynthesis. Energy Metabolism (concept of free energy); Principles of Thermodynamics; Kinetics, Dissociation and Association Constants; Bioenergetics.

12

II

CarbohydratesStructure and Classification of Mono, Di and Polysaccharides – Occurrence –Isolation – Purification – Properties and Biological Reactions. Structural Features of Homoglycans, Heteroglycans and Complex Carbohydrates. Glycolysis and TCA Cycle; Glycogen Breakdown and Synthesis; Gluconeogenesis; Interconversion of Hexoses and Pentoses.

12

III

Proteins and Lipids Proteins: Classification– Primary Structure of Proteins – Structural Comparison at Secondary and Tertiary levels (Ramchandran Map) – Conformation of Proteins and Polypeptides (Secondary, Tertiary, Quaternary and Domain Structure) – Protein Folding - Biophysical and Cellular Aspects. Lipids: Structure and Classification – Properties –Oxidation of Fatty acids – Biosynthesis of Fatty acids – Triglycerides.

12

IV

Nucleic AcidsClassification – Structure and Properties – Biological Significance of Double Strand – Sequence Dependent Variation in the Shape of DNA. Physical Properties of Double Stranded DNA, Structure of Double Stranded DNA (B, A, C, D, T and Z DNA) - Types of RNAs and their Biological Significance - DNA Bending – DNA Supercoiling, Secondary and Tertiary Structural Features and their Analysis - Biosynthesis of Purines and Pyrimidines

12

V

EnzymesClassification – Regulation of Enzymatic Activity - Enzyme Kinetics - Active Sites; Coenzymes – Coenzymes Interactions, Activators and Inhibitors – Isoenzymes – Allosteric Enzymes – Ribozyme – Hammer Head – Hair Pin and other Ribozymes –Abzyme: Structure and Drug Targets (Enzymes and Receptors).

12

Text Books:1. Principles of Biochemistry – Lehninger , Nelson, Cox, CBS publishers2. Fundamentals of Biochemistry – Voet et al., John Wiley and Sons, Inc.Reference Books:1. Biochemistry (3rd Edition) - Christopher K. Mathews, Kensal E. van Holde, Kevin G. Ahern, Pearson Education. 2. Principles of Biochemistry (8th Edition) – Smith et al., McGraw – Hill International book Company. 3. Biochemistry – Zubay, WCB publishers. 4. Harper’s Biochemistry – R.K.Murray, D.K. Granner, P.A. Mayes and V.W. Rodwell, Prentice-Hall International. 5. Biochemistry (6th Ed.) – J.M. Berg, J.L. Tymoczko and L. Stryer, W H Freeman and Company, NY.

Course Designed by Verified by HOD Checked by Approved byDr. MOHAMMED RAFIQKHAN K


Programme Code: DSE Programme Title: Master of Science in Biotechnology

Course Code: 19BTP04 ACourse Title Batch: 2019-2021

PLANT AND ANIMAL SYSTEM PHYSIOLOGY Semester: I


Course Objective1. To make the students understand Plant and Animal cells, its functions and also to provide the basics of Plant and Animal Cell Physiology.2. To understand about the photosynthesis ,translocation biosynthesis , phytochromes and biological clocks.Course Outcomes (CO)

K1 CO1 To understand about biosynthesis of secondary metabolite, biotic and abiotic stress.

K2 CO2 To understand about blood and cardiovascular system with their anatomy, specialized tissues.

K3 CO3 To understand about anatomy, structure and function of lungs, Nervous system and sense organs.

K4 CO4 To understand about Excretory system , endocrinology and reproduction in regulation of body

Mapping of Outcomes


CO1 S M M L

CO2 S M L L

CO3 S M L L

CO4 S S M L



PLANT AND ANIMAL SYSTEM PHYSIOLOGY


I

Plant MetabolismPhotosynthesis - Electron Transport - CO2 fixation- C3, C4 and CAM pathways - Solute Translocation through Xylem and Phloem. Biosynthesis – Storage - Breakdown and Transport - Physiological Effects and Mechanisms of Action of cytokinin and auxins Structure, Function–of Cryptochromes and Phototropins; - Photoperiodism and Biological Clocks.

10

IISecondary Metabolites and Stress physiologyBiosynthesis of Terpenoids – Steroids – Alkaloids - Flavonoids and Phenolic Compounds and their roles. Responses of Plants to Biotic (Pathogen and Insects) and Abiotic (Water, Temperature and Salt) Stresses

9

IIIMuscular System: Classification – Structure and Function. Blood and Cardiovascular System: Blood Corpuscles - Blood Groups – Haemoglobin - haemostasis. Comparative Anatomy of Heart Structure, Myogenic Heart - Specialized Tissue - Cardiac Cycle - Heart as a Pump - Blood Pressure.

10

IVRespiratory System: Anatomy and Structure of Lungs - Transport of Gases - Exchange of Gases – Waste Elimination - Neural and Chemical Regulation of Respiration. Nervous System: Types - Neurons – Synapse. Sense organs: Vision - hearing and tactile response.

9

V

Excretory System: Anatomy and Structure of Urinary System – Micturition - regulation of electrolyte balance and acid-base balance. Endocrinology and Reproduction: Endocrine glands - basic mechanism of Hormone Action - Hormones and Diseases. Reproductive Processes - Neuroendocrine Regulation.

10

Text Book:1. Frank B Salisbury and Cleon Wross, “Plant Physiology”, CBS Publishers and Distributors, New Delhi.2. Neilson K.S, “Animal Physiology”, Cambridge University Press, Pergamon Press, Oxford.

Reference Books 1. Roy G. Noggle and George J. Friltz, “Introductory Plant physiology”, Prentice-Hall of

India Pvt.Ltd, New Delhi.2. Verma, V. 2007. Text book of plant physiology. Ane books pvt. Ltd. 3. Malick, C. P. 2010. Text book of plant physiology. Kalyani publisher. 4. Mukherji, S. 1995. Text book of plant physiology. Tata McGraw-hill education private

Ltd. 5. Singh, Sp. 2001. Text book of plant physiology. Academic internet publishers.


Dr. K. MANIMEKALAI


Programme Code: DSE Programme Title: Master of Science in Biotechnology

Course Code: 19BTP04 B Course Title Batch: 2019-2021OCCUPATIONAL HEALTH AND

INDUSTRIAL SAFETYSemester: I


Course Objective1.The areas covered under this study helps to get knowledge on existing policies/directives

for Industrial Occupational Health and Safety Management. It also gives emphasis on prevention and control methods.

2. Educate students about how to reduce work place hazards and to encourage thestandard of Safety, Health & Environment Programme,


K1 CO1 Will be able to identify hazards in the home or workplace that pose a danger or threat to their safety or health, or that of others.

K2 CO2 Should be able to know how to control unsafe or unhealthy hazards and propose methods to eliminate the hazard

K3 CO3 Should be able to discuss the role of health and safety in the workplace pertaining to the responsibilities of workers, managers, supervisors. To explain a comprehension of the changes created by WHMIS legislation in everyday life.

K4 CO4 Will be able to identify the decisions required to maintain protection of the environment, home and workplace as well as personal health and safety.

Mapping of Outcomes


CO1 S S M SCO2 S L S LCO3 S M S MCO4 M S S S



OCCUPATIONAL HEALTH AND INDUSTRIAL SAFETYUnit No. Topics Hours

I

Parameters of safetyConcept of safety organization and Management - Safety Regulations. Definition and Role of Ergonomics in Designing Work-Place. Factors affecting the conditions of occupational and Industrial safety.

9

II

Work EnvironmentEffects of Light -Ventilation – Vibration – Noise. The Work Physiology and their Relevance to Safety - Performance Evaluation of Man - Environment systems.

9

III

Occupational Health and SafetyOccupational Health and Hazards – Physical, Chemical and Biological hazards. Occupational Diseases, their Prevention and Control. Health Protection Measures for Workers. Principles of Arthropod Control.

10

IV

Health Education Medical First-Aid and Management of Medical Emergencies Industrial Safety management TechniquesIndustrial Safety Standards. Accidents-Definition, Frequency Rate, Prevention and Control. Types of accidents -Work Study - Method of Study and Measurement. Measurement of Skills. Safety - Cost of Expenses.

10

V

Case Study Principles of Functions in Safety Management - Case Study - Visit to an Industry - Preparation of report on safety measures followed in Airport/Industry - .Distillery, Paper, Tannery, Pharmaceutical Industries.

10

Text Book:1.Kolluru R.V, “Environmental Strategies–Hand Book”, Mc Graw Hill Inc., New York.Reference Books:1. Walsh W and Russell L, “A B C of Industrial Safety”, Pitma Publishing, United Kingdom. 2. Hommadi A.H, “Environmental and Industrial Safety”, I.B.B Publication, New Delhi.3. 4.Risk assessment- A Practical Guide, 1993, Institution of Occupational Safetyand Health, United Kingdom5.Fundamentals of Occupational Safety and Health by James Kohn and Mark A. Friend published by ABS Consulting, 2003 - Technology & Engineering


Dr. S.G. ANTONY GODSON


Programme Code: SEC Programme Title: Master of Science in Biotechnology

Course Code: 19BTP05Course Title Batch: 2019-2021

PRACTICAL I - CELL AND MOLECULAR BIOLOGY Semester: I


Course ObjectiveTo develop practical skills such as identification of cell types, cellular component and cell

division, etc. Course Outcomes (CO)

K1 CO1 To know basic Concept principle and application of cell and molecular biology

K2 CO2 Students will be able to differentiate the cells of various living organisms and get awareness of physiological processes of cell e.g. cell divisions.

K3 CO3 Students will be able to observe and correctly identify different cell types, cellular structures using different microscopic techniques.

K4 CO4 Independently execute a laboratory experiment using the standard methods and techniques in molecular biology, with the appropriate analysis and interpretation of results obtained.

Mapping of Outcomes


CO1 S M L L

CO2 M S M M

CO3 S M S M

CO4 M S M S



19BTU05 PRACTICAL I -CELL AND MOLECULAR BIOLOGY I


1. Microscopy – Working principle of Bright Field Microscope2. Identification of Cell Types – Plant, Animal and Microbes.3. Fractionation of Cellular components – Nucleus, Mitochondria,

Chloroplast.4. Mitotic Preparation - Onion Root Tip.5. Identification of Barr Bodies.6. Mounting of Polytene Chromosome from Chironomous Larvae.7. Identification of Mutant s - Physical and Chemical Methods8. Auxotrophic selection of mutants by replica plating technique9. Sucrose Fractionation of Castor Bean

Lipid Solubility of Membranes

Reference Books:

1. Joseph Sambrook, Fritsch E.F, Tom M and Chris N, “Molecular Cloning: A Laboratory Manual”, Cold Spring Harbor Laboratory, New York.

2. Cappuccino, P., & Sherman, D. (2004). Microbiology-A Lab Manual. (7th ed.) Singapore: Pearson Educatio3. Dubey, R., & Maheswari, E. (2004). Practical Microbiology. New Delhi: S. Chand & Co. 4. Chitanya K.V.”Cell and Molecular Biology –Lab manual.PHI Learninig pvt Ltd.5. Palsamy”Laboratory manual on Biotechnology.Rastogi publications.


Dr. S. KOMATHI




PRACTICAL II - APPLIED MICROBIOLOGY Semester: I


Course Objective1. To enable the students to understand the basic techniques and applications in Microbiology,.. 2.. To know about the fundamentals laboratory apparatusCourse Outcomes (CO)

K1 CO1 To understand principles of microscopy

K2 CO2 To establish protocol for the isolation of microbes and staining methods

K3 CO3 To prepare analytical quality solutions, buffers and dilution series and know it calibration, understand principles of calibration.

K4 CO4 To select and optimize media for maximum production of microbial metabolites .

Mapping of Outcomes


CO1 S S M S

CO2 S M S L

CO3 S S M M

CO4 S S S S



19BTU06 PRACTICAL II- APPLIED MICROBIOLOGY I


1.Microscopy- Light, SEM, TEM, Fluorescent, Inverted 2.Microscope.Isolation of Microbes from Soil, Water and Contaminated Food. 3.Pure culture Techniques- Streak plate, pour plate, Spread plate,

decimal dilution.4.Staining of Bacteria: Simple, Gram’s, Endospore, Flagellar Staining.5.Staining of Fungi - Lacto Phenol Cotton Blue Method.6.IMVIC Test.8.Hydrogen sulphide test.9.Oxidase test.10.Catalase Test.11.Urease test.12.TSI test13.Carbohydrate fermentation.14.Bacterial Growth curve15.Effect of pH and Temperature on Bacterial Growth by Turbidity

method16. MPN test17.Antibiotic sensitivity test.

1. Subbarao N. S. 2006. Soil Microbiology. (4th Edition of Soil microbiology and Plant growth). Oxford & IBH, New Delhi

2. S. Rajan. Medical Microbiology by MJP Publishers


Dr. V. VIJAYALAKSHMI



Course Code: 19BTP07 Course Title Batch: 2019-2021PRACTICAL III - BIOCHEMISTRY Semester: I


Course Objective

1. To enable the students to understand the basic techniques and applications in

Biochemistry.

2. To prepare the analytical quality solutions, buffers and dilution series and know it

calibration, understand principles of calibration


K1 CO1 To establish and understand the importance of Biomolecules in Living organisms

K2 CO2 To protocol for the isolation of microbes and staining methods

K3 CO3 To prepare analytical quality solutions, buffers and dilution series and know it calibration, understand principles of calibration.

K4 CO4 To select and optimize media for maximum production of microbial metabolites .

Mapping of Outcomes


CO1 S S S S

CO2 S M S S

CO3 S S S M

CO4 M S S S



19BTU06 PRACTICAL III -BIOCHEMISTRY I


1. Estimation of Carbohydrates by Anthrone method. 2. Estimation of Acid Value, Saponification value, Iodine number of

fat. 3. Estimation of Amino Acids by Ninhydrin Method4. Protein Estimation by Lowry’s Method and Bradford method. 5. Separation of LDH Isozymes from Serum by SDS-PAGE. 6. Extraction and purification of peroxidase from soy bean seeds7. Assay of amylase activity and determination of enzyme kinetic

parameters - Km, Vmax and Kcat8. Paper Chromatography - Separation of Plant Pigments. 9. Thin Layer Chromatography - Separation of Amino Acids. 10. Ion Exchange Chromatography. 11. Gel permeation Chromatography.12. Estimation of Ascorbic Acid.

Estimation of Calcium and Iron

Reference Books:

1. Laboratory Manual of Biochemistry by J. Jayaraman, Published by Willy Eastern. 1981. Edition: 2.

2. Protein Methods by Daniel M. Bollag et al. Published by Wiley-Liss, Inc. 1996. Edition: 2.

3. Biochemical Methods by S. Sadasivam and A. Manickam, Published by Reprint New age international (P) Ltd. 1996. Edition: 2.

4. An Introduction to practical Biochemistry by David T Plummer, Published by Tata Mcgraw hill Publication. 1971. Reprinted 2004.




Course Code: 19BTP09

Course Title Batch: 2019-2021 GENETIC ENGINEERING Semester: II


Course Objective To impart knowledge on genetic manipulation techniques and its application in producing

GMO’s.


K1 CO1 To study the structure of gene and genome organization and expression in prokaryotes and eukaryotes.

K2 CO2 Understanding of types and functions of enzymes in genetic engineering

K3 CO3 The students will have knowledge of plant and animal vectors as a tool in genetic engineering.

K4 CO4 Understanding about advanced molecular techniques in biotechnology.

Mapping of Outcomes


CO1 S M S L

CO2 S S M L

CO3 S S S M

CO4 M S M S



GENETIC ENGINEERING


IGene and GenomeGenome Organization - C - Value Paradox; Human Genome Project and Genome Sequencing Methods - Maxam and Gilbert, Sanger’s method, Pyrosequencing, Automated DNA sequencing, N

12

II

Enzymes in Genetic EngineeringNucleases: Exonucleases and Endonucleases, Restriction Enzymes (Type I, Type II, Type III, Type IV & Type V), RNases; Methylases: CpG Methylase , Dam Methylase, Dcm Methylase, Polymerases: DNA Pol I, Klenow Fragments, Reverse Transcriptase, Taq & Pfu Polymerases; Ligases: T4 DNA Ligase, E.coli DNA Ligase, T4 RNA Ligase; Topoisomerases: Type I(A, B) & Type II(A,B); End Modifying Enzymes: Terminal Transferase, T4 Polynucleotide Kinase, Alkaline Phosphatases

12

III

Vectors Steps in Gene Cloning – Host Cell Types – Prokaryotic Hosts, Eukaryotic Hosts – Vectors - Plasmid Vectors – pBR322 – pUC vectors – Bacteriophage Vectors – Lambda and M13 vectors; Hybrid Vectors – Cosmids – Phagemids. Eukaryotic Vectors – Yeast Vectors – YAC and BAC. Plant Vectors – CaMV– TMV –Animal Vectors – SV40 – Retrovirus – Adenovirus.

12

IV

Advanced Molecular TechniquesGenomic Library Construction – cDNA Library Construction – Chromosome Walking and Jumping. Screening of Recombinants –. Blotting Techniques - PCR – Real time PCR - Genome editing – ZFN, TALENs, CRISPR Tool Site Directed Mutagenesis and Protein Engineering – in situ hybridization and site directed mutagenesis.

12

VGene therapy Gene Therapy – Germline, Somatic Cell Line – In vivo – Ex vivo – Anti-Sense Gene Therapy and Embryo Gene Therapy –RNA interference (RNAi). Gene knockout Genetic Counseling.

12

Text Book:1. Primrose S.B, “Molecular Biotechnology”, Blackwell Scientific Publishers, Oxford.Reference Books:1. Mickloss D.A and Freyar G.A, “DNA Science: A First Course in Recombinant Technology”, Cold Spring

Harbor Laboratory Press, New York.2. Walker M.R and Rapley R, “Route Maps in Gene Technology”, Blackwell Science Ltd, Oxford.3. Desmond S.T. Nicholl. “An Introduction to Genetic Engineering”Third Edition Cambridge University Press4. Senthilkumar Sadasivama and Mohammed Jabir (2008). IPR, Biosafety and Biotechnology Management,

Jasen Publications, India5.Brown T.A”Gene cloning and DNA analysis” Blackwell Scientific Publishers, Oxford

Course Designed by Verified by HOD Checked by Approved byDr. G. RAJALAKSHMI




RESEARCH METHODOLOGY AND BIOSTATISTICS Semester: II


Course ObjectiveTo enable the students to understand the basic knowledge on thesis writing and problem solving in research


K1 CO1 To understand the research , collection of data’s, data characteristics, and thesis writing

K2 CO2 To understand the exact method of data analysis for the problem under investigation - about publication of manuscript- databases, journal information and impact factor

K3 CO3 How to use and interpret results of descriptive statistical methods

K4 CO4 To know principal methods of statistical interference and design for effective use in analysing biotechnological results

Mapping of Outcomes


CO1 S M S M

CO2 S S S S

CO3 S S M S

CO4 S S S S



RESEARCH METHODOLOGY AND BIOSTATISTICSUnit No. Topics Hours

I

Introduction to research methodologyWhat is Research? Basic and Applied Research - Essential steps in Research - Defining the Research Problem - Research/Experimental Design. Literature Collection - Literature Citation. Research Report – Components – Format of Thesis and Dissertation – Manuscript/Research Article – Review Monographs – Bibliography. Significance of Research, Teaching Learning, Evaluation and Monitoring Process.

12

II

Databases and Information Retrieval Journals - Standard of Research Journals – Plagiarism – Copyrights - Impact Factor - Citation Index – Bibliometrics – Google Scholar Citation. Access to Archives and Databases – Science Direct – Sciverse– Pubmed - National Informatics Center Network Services – Online Data Base Library.

12

III

BiostatisticsDefinitions - Scope of Biostatistics – Classification and Tabulation of Data – Graphical and Diagrammatic Representation – Scale Diagrams – Histograms – Frequency Polygon - Frequency Curves. Measures of Central Tendency – Arithmetic Mean – Median and Mode. Calculation of Mean – Median – Mode in Series of Individual Observation Discrete Series – Continuous Open End Classes.

12

IV

Measures of DispersionStandard deviation and Range, Chi – Square Test, Student - t Test, Regression, Correlation, One Way and Two Way ANOVA. Experimentation Design: Completely Randomized Design – Factorial Design – Plackett Burman Designs – Response Surface Methodology: Central Composite Designs (adapted from Montgomery) and Box-Behnken design – Taguchi statistical method.

12

V

Proportion Data and AnalysisExamples of Proportion data - MPM-sterility testing of medicines - animal toxicity infection and immunization studies (e.g., LD50, ED50, PD50) –statistical treatment to proportion data.Introduction to Count data – Examples (bacterial cell count, radioactivity count, colony and plaque count, etc.). Statistical treatment to count data Poisson distribution - standard error -confidence limits of counts. Application of statistical software for biological research.

12

Text Book:1. Karthikeyan S, Chaturvedi R M and Bhonsale R M, “Comprehensive Textbook of

Biostatistics and Research Methodology”, Bhalani Publishing House, New Delhi.2. Kothari C R, “Research Methodology Methods & Techniques”, Second Edition, New

Age International publishers, New Delhi

Reference Books: 1. How to Write and Publish a Scientific Paper? ; Robert A. Day, Barbara Gastel ; 6th edition; Cambridge : Cambridge University ; 2006. 2. Research Methodology: A step by step guide for beginners byRanjit Kumar, Third Edition, Ranjit Kumar University of Western Australia, SAGE Publications Ltd3. Research Methodology by R. Pannerselvam, Publisher:PHI Learning, 2004, Edition: Second Edition.


4. Introduction to Bio-statistics and Research methods by P.S.S Sundar Rao & J. Richard, Publisher: PHI Learning, 2012, Edition: fifth edition.5. Research Methods in Community Medicine: Surveys, Epidemiological Research, Programme Evaluation, Clinical Trials, Sixth Edition, by J. H. Abramson, Z. H. Abramson




Course Code: 19BTP11 Course Title Batch: 2019-2021BIOPROCESS TECHNOLOGY Semester: I


Course Objective1. To impart knowledge on Design and Operation of Fermentation Processes with its entire prerequisite and to endow the students with the basics of Microbial Kinetics, Metabolic Stoichiometry and Energetics.2. To evaluate design features and the instrumentation and control of bioreactors..Course Outcomes (CO)

K1 CO1 To learn about fermentation and usage of microorganisms in the production of fermented products.

K2 CO2 How batch, fed-batch and continuous flow bioreactors operate and be able to analyze data from these types of bioreactors

K3 CO3 To evaluate design features and the instrumentation and control of bioreactors. K4 CO4 To evaluate about the down stream processing in the product recovery

Mapping of Outcomes


CO1 S S M L

CO2 S M L S

CO3 S S S M

CO4 S S M L


BIOPROCESS TECHNOLOGY



Unit I

Introduction to Bioprocess Engineering Fermentation – a Historical Perspective. Sources of Industrially Important Microbes for Fermented Products – Antibiotics – Vitamins – Enzymes – Organic Acids. Biotechnology and Bioprocess Engineering – Bioprocess Regulatory Constraints – Cell Construction. Substrate for Fermentation – Media Formulation - Cell Nutrients. Stoichiometry of Microbial Growth and Product Formation.

12

Unit II

Bioreactor - Designs Introduction to Fermentation Process – Types of Fermentation – Batch – Fed Batch – Continuous. Sterilization, Thermal Death Kinetics. Design of Continuous Sterilization Process, Fibrous Filters. Bioreactor Design, Parts and their Functions, Alternative Vessel Designs – CSTR – Tower – Airlift – Loop Jet – Bubble Column – Packed Bed.

12

Unit III

Kinetic Studies Microbiology of Industrial Fermentation – Fermentation Kinetics – Rheological properties of the Medium – Theory of Mixing. Oxygen Transfer Rate – Oxygen Transfer Coefficient and Correlation. Biological Heat Transfer and Heat Transfer Coefficient – Mass Transfer.

12

Unit IV

Control and Monitoring Different types of Instrumentation, Common Measurement and Control Systems – Additional Sensors – Feedback Control – pH – Temperature – Agitation – Aeration – O2 – Anti Foam – Baffles – Sparger and On/Off Valves - PID Control – Computers in Bioprocess Control Systems – Biosensors in Bioprocess Monitoring and Control.

12

Unit V

Downstream Processing Filtration – Removal of Microbial Cells – Cell Disruption: Enzymatic, Chemical and Physical Methods; Purification of Fermentation Products - Precipitation Methods, Membrane Process. Centrifugation – Ultracentrifugation; Chromatography - Ion Exchange and Gel Permeation Chromatography – HPLC. Crystallization – Drying – Lyophilisation.

12

Text Book:1. Michael L. Shuler and FikretKarg, “Bioprocess Engineering Basic concepts”, Prentice Hall International services, New Jersey. Reference Books:1. El-Mansi E.M.T and Bryce C.F.A, “Fermentation Microbiology and Biotechnology”, Taylor & Francis, London. 2. Wulf Crueger and Anneliese Crueger, “Biotechnology-A Textbook of Industrial Microbiology”, Panima Publishing Corporation, New Delhi.Principles of fermentation technology by P.F. Stanbury and A.Whitaker, Pergamon press. Second edition. 2005.3. Fermentation microbiology and Biotechnology. Second edition, edited by El-.Mansi, C.F.A. Bryce, A.L. Demain, A.R. Allman. Taylor and Francis, 2007.4. Introduction to Biochemical engineering by D.G.Rao, McGraw-Hill publications, I edition, 2007.5. Industrial Microbiology by Prescott and Dunns 4th edition edited by Gerald Reed, Chapman & Hall publications 2007.Industrial microbiology by L. E. Cassida Jr.


Dr. V. VIJAYALAKSHMI



Course Code: 19BTP12 A Course Title Batch: 2019-2021BIOETHICS, BIOSAFETY AND IPR Semester: II


Course Objective1. To provide basic knowledge on intellectual property rights and their • implications in biological research and product development;2. To become familiar with India’s IPR Policy;Course Outcomes (CO)

K1 CO1 Understand the rationale for and against IPR and especially patents;

K2 CO2 Understand why India has adopted an IPR Policy and be familiar with broad outline of patent regulations;

K3 CO3 Understand different types of intellectual property rights in general and protection of products derived from biotechnology research and issues related to application and obtaining patents;

K4 CO4 Gain knowledge of biosafety and risk assessment of products derived from recombinant DNA research and environmental release of genetically modi ed organisms, national and international regulations;

Mapping of Outcomes


CO1 S S M M

CO2 S S M M

CO3 S S S S

CO4 S S M M



BIOETHICS, BIOSAFETY AND IPRUnit No. Topics Hours

I

Introduction to Bioethics in BiotechnologyBiotechnology and Ethics –Benefits And Risks Of Genetic Engineering – Ethical Aspects of Genetic Testing and Genetic counseling - Ethical Implications of Cloning: Reproductive Cloning, Therapeutic Cloning; Ethical, Legal and socioeconomic aspects of Gene Therapy (ELSI), Germ Line, Somatic, Embryonic and Adult Stem Cell Research.

12

II

Biosafety Regulations Guidelines and Regulations (National and International) - Cartagena Protocol on Biosafety –The Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). GMO’s and LMO’s, RCGM, GEAC etc for GMO application in food and agriculture; Environmental release of GMO’s

12

III

Biosafety and Institutional Biosafety CommitteesIntroduction to Biosafety its Issues in Biotechnology – Risk Assessment and Risk Management – Safety Protocols: Risk Groups – Biosafety Levels –Biosafety Containment.

12

IV

Intellectual Property RightsIPR and its Different types Patents, Trade Mark, Trade Secret, Copy Rights, Designs Geographic Indications, Plant breeders rights - IP relevance to biotechnology Protection of New GMO’s.

12

V

Patent What can and what cannot be patented? -- Patent Application -- International Patenting and Patent co-operation treaty – Revocation of Patent – Patenting of biological material Patenting in India - FlavrSavr™ Tomato as a Model Case - Biopiracy and Case Studies on Patents (Basmati Rice, Turmeric, and Neem).

12

Text Book: 1. Shaleesha A. Stanley, “Bioethics”, Wisdom Educational Service, Chennai.Reference Books: 1.Jose Cbelli., Robert P. Lanza., Keith H.S., Michael D. West, “Principles of Cloning”, Academic Press, USA.2.Biological safety: principles and practices by fleming and Hunt. American society for Microbiology. 3.Introduction to Plant Biotechnology by H.S. Chawla Ganguli, P. (2001). Intellectual Property Rights: Unleashing the Knowledge Economy. New Delhi: Tata McGraw-Hill Pub.4.National IPR Policy, Department of Industrial Policy & Promotion, Ministry of Commerce, GoI5.Complete Reference to Intellectual Property Rights Laws. (2007). Snow White Publication Oct.6.Kuhse, H. (2010). Bioethics: an Anthology. Malden, MA: Blackwell.7. O ce of the Controller General of Patents, Design & Trademarks; Department of Industrial Policy & Promotion; Ministry of Commerce & Industry; Government of India. http://www.ipindia.nic.in/


Mrs. R.GOHILA



Course Code: 19BTP12 B

Course Title Batch: 2019-2021GENOMICS AND PROTEOMICS Semester: I


Course Objective1. To gain an appreciation for the field of systems biology.

2. To understand and learn the technical details of several current experiments or

technologies used in the field of systems biology.

3. To understand some of the larger questions and issues with systems biology and large-scale data collection and analysis.


K1 CO1 Explain the importance of bioinformatics in systems biologyK2 CO2 Discuss the use of genes and genomes data in systems biology

K3 CO3 Integrate the omics data for networkingK4 CO4 Apply the appropriate tools in systems biology for modelling

Mapping of Outcomes


CO1 S S M M

CO2 S S M L

CO3 S S S S

CO4 S S S S


GENOMICS AND PROTEOMICS


Unit No.

Topics Hours

I

Genome Mapping – Mapping Genomes, Genetic and Physical Maps, Sequencing Genomes, Methodology for DNA Sequencing, Assembly of a Contiguous DNA Sequence, Genome Analysis DNA Sequencing databases, Sequence analysis programs, Pairwise sequence alignment, Multiple sequence alignment, DNA sequencing, Genomic sequencing, Sequencing cDNA Libraries of expressed genes

12

II

Gene Prediction and Genome Rearrangement Introduction, gene prediction methods and tools, Applications to Complex GenomesComparative Genomics – Completed genomes, Sequence assembly and gene identification,functional classification of genes.

12

III

Protein classification Principles of classification: Based on structural features, Phylogenetic relationship. Protein structure prediction Use of sequence pattern, leucine zipper, coiled coil, transmembrane, signal peptide, cleavage site. Secondary structure prediction: Chou – Fasman / GOR method, Neural network, nearest neighbor method, tertiary structure prediction, threading profile, contact potential, modeling.

12

IV

Bioanalysis of protein and peptides Complex protein and peptide mixtures, Extracting proteins from biological samples, Protein separation before digestion: 1D and 2 D Electrophoresis, Immobilized pH gradient, Sample preparation, First dimension criteria, second dimension criteria, Stabilization, Detecting protein on gel: Electro blot, Image analysis, Digital imaging, Spot detection and quantification, Gel matching. Data Analysis – Database for 2D gel

12

V

Tool of Proteomics Mass Spectrometry for protein and peptide analysis: MALDI-TOF Analyzers, ESI Tandem MS instrument, Tandem Mass Analyzers, The Triple Quadrupole Mass Analyzer, The Ion Trap Mass Analyzer, Q-TOF & Fourier Transform–Ion Cyclotron Resonance, MS Instrument.

12

Text Book:1. Michael L. Shuler and FikretKarg, “Bioprocess Engineering Basic concepts”, Prentice Hall International services, New Jersey. Reference Books:1. El-Mansi E.M.T and Bryce C.F.A, “Fermentation Microbiology and Biotechnology”, Taylor & Francis, London. 2. Wulf Crueger and Anneliese Crueger, “Biotechnology-A Textbook of Industrial Microbiology”, Panima Publishing Corporation, New Delhi.3. Bioinformatics and Functional Genomics by Pevsner, J. A. John Wiley & Sons, Inc., USA(2009).3. Ideker et al. A new approach to decoding life: Systems Biology. Annual Review on Genomics and Human Genetics 2001, 2: 343-372.4. Ideker et al. Integrated Genomic and Proteomic Analyses of a Systematically Perturbed Metabolic Network. Science, 2001, 292: 929-934.


Dr.K.MANIMEKALAI



Course Code: 19BTP12 A Course Title Batch: 2019-2021BIOETHICS, BIOSAFETY AND IPR Semester: II


Course Objective1. To provide basic knowledge on intellectual property rights and their • implications in biological research and product development;2. To become familiar with India’s IPR Policy;Course Outcomes (CO)

K1 CO1 Understand the rationale for and against IPR and especially patents;

K2 CO2 Understand why India has adopted an IPR Policy and be familiar with broad outline of patent regulations;

K3 CO3 Understand different types of intellectual property rights in general and protection of products derived from biotechnology research and issues related to application and obtaining patents;

K4 CO4 Gain knowledge of biosafety and risk assessment of products derived from recombinant DNA research and environmental release of genetically modi ed organisms, national and international regulations;

Mapping of Outcomes


CO1 S S M M

CO2 S S M M

CO3 S S S S

CO4 S S M M



BIOETHICS, BIOSAFETY AND IPRUnit No. Topics Hours

I

Introduction to Bioethics in BiotechnologyBiotechnology and Ethics –Benefits And Risks Of Genetic Engineering – Ethical Aspects of Genetic Testing and Genetic counseling - Ethical Implications of Cloning: Reproductive Cloning, Therapeutic Cloning; Ethical, Legal and socioeconomic aspects of Gene Therapy (ELSI), Germ Line, Somatic, Embryonic and Adult Stem Cell Research.

10

II

Biosafety Regulations Guidelines and Regulations (National and International) - Cartagena Protocol on Biosafety –The Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). GMO’s and LMO’s, RCGM, GEAC etc for GMO application in food and agriculture; Environmental release of GMO’s

10

III

Biosafety and Institutional Biosafety CommitteesIntroduction to Biosafety its Issues in Biotechnology – Risk Assessment and Risk Management – Safety Protocols: Risk Groups – Biosafety Levels –Biosafety Containment.

9

IV

Intellectual Property RightsIPR and its Different types Patents, Trade Mark, Trade Secret, Copy Rights, Designs Geographic Indications, Plant breeders rights - IP relevance to biotechnology Protection of New GMO’s.

9

V

Patent What can and what cannot be patented? -- Patent Application -- International Patenting and Patent co-operation treaty – Revocation of Patent – Patenting of biological material Patenting in India - FlavrSavr™ Tomato as a Model Case - Biopiracy and Case Studies on Patents (Basmati Rice, Turmeric, and Neem).

10

Text Book: 1. Shaleesha A. Stanley, “Bioethics”, Wisdom Educational Service, Chennai.Reference Books: 1.Jose Cbelli., Robert P. Lanza., Keith H.S., Michael D. West, “Principles of Cloning”, Academic Press, USA.2.Biological safety: principles and practices by fleming and Hunt. American society for Microbiology. 3.Introduction to Plant Biotechnology by H.S. Chawla Ganguli, P. (2001). Intellectual Property Rights: Unleashing the Knowledge Economy. New Delhi: Tata McGraw-Hill Pub.4.National IPR Policy, Department of Industrial Policy & Promotion, Ministry of Commerce, GoI5.Complete Reference to Intellectual Property Rights Laws. (2007). Snow White Publication Oct.6.Kuhse, H. (2010). Bioethics: an Anthology. Malden, MA: Blackwell.7. O ce of the Controller General of Patents, Design & Trademarks; Department of Industrial Policy & Promotion; Ministry of Commerce & Industry; Government of India. http://www.ipindia.nic.in/


Mrs. R.GOHILA




PRACTICAL IV- GENETIC ENGINEERING Semester: II


Course ObjectiveTo enable the students to understand the techniques involved genetic material

isolation and various methodology in Genetic Engineering.


K1 CO1 To know basic of rDNA technology, Concept and principle and application of genetic engineering.

K2 CO2 Students will be able to observe Gel electrophoresis.

K3 CO3 To explain principles, material and methodology of techniques involved in rDNA technology, , blotting techniques, sequencing methods, PCR. DNA fingerprinting and Reporter gene assays

K4 CO4 Independently execute a laboratory experiment using the standard methods and techniques in molecular biology, with the appropriate analysis and interpretation of results obtained.

Mapping of Outcomes


CO1 S S S S

CO2 S S S S

CO3 S M S S

CO4 S S M S


PRACTICAL IV - GENETIC ENGINEERING



1. Isolation of Genomic DNA from Plant Tissue 2. Isolation of Genomic DNA from Bacteria3. Isolation of Genomic DNA from Animal Tissue4. Isolation of Genomic DNA from Human blood5. Isolation of Plasmid DNA from Bacteria. 6. Restriction Digestion 7. Ligation. 8. Transformation in E.coli, 9. Polymerase Chain Reaction. 10. Southern Hybridization using Non-Radioactive Detection. 11. Western Blotting.12. Reporter gene assay - GUS gene

Reference Books:

1.John Vennison”Laboratry manual for Genetic Engineering” PH Publisher. 2.Aksan Kurnaz”Techniques in Genetic Engineering”CRC Press3. Dubey, R., & Maheswari, E. (2004). Practical Microbiology. New Delhi: S. Chand & Co. 4.Chitanya K.V.”Cell and Molecular Biology –Lab manual.PHI Learninig pvt Ltd.5.Green and Sambrook”Molecular Cloninig-Lab Manual 4th Edition


Dr.G.RAJALAKSHMI

Programme Code: SEC Programme Title: Master of Science In Biotechnology



PRACTICAL V - BIOPROCESS TECHNOLOGY Semester: II


Course Objective1. Aims to enhance the knowledge about the operation of fermentor and working design.

2. Enable the students to train individually to produce industrially important products.


K1 CO1 To know basic of fermentation technology, Concept ,principle and application of bioprocesses technology.

K2 CO2 Students will be able to fermentor design and working principles

K3 CO3 To explain principles, material and methodology of citric Acid Production ,enzyme production, wine production ,antibiotic Production.Isolation and secondary screening of industrially

K4 CO4 Independently students execute the isolation and secondary screening of industrially important microbes in fermentation technology field.

Mapping of Outcomes


CO1 S M S S

CO2 S S S S

CO3 S S S S

CO4 S S S S


PRACTICAL V - BIOPROCESS TECHNOLOGY Unit No. Topics Hours


1. Fermentor – Design and Working Principle. 2. Citric Acid Production. 3. Amylase Production. 4. Wine Production.5. Antibiotic Production and its Bioassay6. Culture optimization (pH, Temperature, Carbon & Nitrogen sources).7. Bioassay of Antibiotics.8. Immobilization of cells for enzyme production.9. Purification of enzymes by salting, dialysis and column chromatography technique

1.Instrumentation, measurement and analysis, II edition, Nakra BC and Chaudhry KK, Tata McGrawHill Publishing Co. Ltd., New Delhi, 2004 2. Fermentation Microbiology and Biotechnology , Mansi El-Mansi and Charlie Bryce, Taylor and Francis Ltd., 2002. 3.Manual of Industrial Microbiology and Biotechnology, III edition, Arnold L. Demain and Julian Davies, ASM press, Washington DC, 1999. 4.Principles of Fermentation Technology by Peter. F. Stanbury, Allan Whitaker, Stephen. J. Hall. Published by Elsevier Science Ltd., reprinted 2007, Edition: 2.5. Bioprocess Engineering Principles by Pauline M. Doran, Published by Elsevier, Reprinted 2006.


Dr.V.VIJAYALAKSHMI

Programme Code: ABE Programme Title: Master of science in BiotechnologyCourse Code: 19BTP15 Course Title Batch: 2019-2021

BIODIVERSITY AND Semester: II


CONSERVATION BIOLOGYHrs/Week: - Credits: 03

Course Objective1. Helps to impart knowledge on ecological and evolutionary interactions between organisms

and their environment2. Aims to enhance the knowledge about the basic concept and structure of ecosystem,

concept of community, animal habitat interaction, many behavioral aspects


K1 CO1 Should be able to have knowledge on basic biodiversity and their distribution in Western Ghats.

K2 CO2 Will be able to know the decreases of animal diversity and species extinction.

K3 CO3 Should be able to understand about conservation biology by In-situ and Ex-situ tools.

K4 CO4 Should be able to explain how to analyze and solve biological problems, by laws and policy in India. And to understand about role of different organization in conservation of animals.

Mapping of Outcomes


CO1 S S M MCO2 S S M SCO3 S S M MCO4 S S S S


BIODIVERSITY AND CONSERVATION BIOLOGYUnit No. Topics

I Biodiversity; Species Concepts; Animal Diversity


What is Biodiversity - Components of Biodiversity (Ecosystem, Genetic and Species diversity) - Assigning values to biodiversity - Species concepts - Animal diversity: (Distribution, inventory, species richness) - Biodiversity Hotspots (Western Ghats, Indo-Burma region).

II

Loss of Animal Diversity, Status of Species ExtinctionsPast rates of Extinctions - Concepts of Island biogeography and extinction rates on Islands - Human induced, Modern and local extinctions - Population reduction - threats to wildlife (examples) - Habitat loss, degradation and fragmentation. Threats to animal diversity in India - Status of species: Rare - endemic and threatened species - Measuring status of species in the wild - IUCN Red list (Assessments and methodologies) - Status of Indian animals.

III

Conservation: Tools In Animal Conservation What is conservation biology? - In situ and Ex situ conservation of Indian animals (Case studies) - Population management - Project Tiger and Elephant - Captive breeding programme - peoples participation in conservation - Successes and failures of conservation actions in India (Case study) - Tools in Conservation: Interpretation of various data on wildlife - GIS - Remote sensing - Landscape model – PVA and CAMP processes.

IV

Animal Laws And Policies In India; Economics of Biodiversity Conservation Wildlife Protection Act of India (1972) - Protected Area network - forest policy - Prevention of cruelty to Animal Act - Convention on Biological diversity - International Trade in endangered species - Zoo policy - Laws and their applications in Zoological parks - wildlife sanctuaries and biosphere reserves - Economics of biodiversity conservation.

V

Conservation Education and Awareness Wildlife / Animal magazines, Journals- How to write popular and Scientific articles - Magazine and Journal information – Wildlife – nature - environment games (examples) – Role of NGO’s and Government organizations in wildlife conservation - Wildlife celebration days in India - Biotechnology in conservation.

Text Book:1. Primack R.B, “Essentials of Conservation Biology”, Sinauer Associates, USA.2. Richard B. Primack. 2002. Essentials of Conservation Biology. 3rd edition, Sinauer Associates, Inc. Publishers. 698 pp. ISBN 0-87893-719-6.

Reference Books:1.Meffe G.K and Carroll C.R, “Principles of Conservation Biology”, Sinauer Associates, USA.2. Groom Bridge B, “Global Biodiversity: Status of the Earth's Living Resources3.Biodiversity and Conservation (Peter J. Bryant), School of Biological Sciences, University of

California


4.Biodiversity (E.O. Wilson, Editor, Frances M. Peter, Associate Editor), National AcademicPress, Washington, D.C.

5.The Wildlife (Protection) Act 1972, The wildlife (as amended up-to 1991)


Dr. S. G. ANTONY GODSON


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