WATERS 2487 Manual

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Waters 2487 Dual Absorbance DetectorOperators Guide

34 Maple Street Milford, MA 01757 WAT048740, Revision 2

NOTICEThe information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This guide is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with or arising from the use of this guide.

19972000 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA. ALL RIGHTS RESERVED. THIS BOOK OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

Alliance, Millennium, and Waters are registered trademarks, and LAC/E, PowerLine, SAT/IN, and TaperSlit are trademarks of Waters Corporation. Microsoft, MS, MS-DOS, Windows, and Windows NT are registered trademarks of Microsoft Corporation. PEEK is a trademark of Victrex Corporation. Teflon is a registered trademark of E.I. du Pont de Nemours and Company. All other trademarks are the sole property of their respective owners.

Attention: The Waters 2487 Dual Absorbance Detector can be used for in vitro

STOP diagnostic applications. This is a highly sensitive instrument. Read the accompanyingoperators guide before using. When using the instrument, follow generally accepted procedures for quality control and methods development. If you observe a change in the retention of a particular compound, in the resolution between two compounds, or in peak shape, immediately take steps to determine the reason for the changes. Until you determine the cause of a change, do not rely upon the results of the separations. Attention: Changes or modifications to this unit not expressly approved by the party

STOP responsible for compliance could void the users authority to operate the equipment.

Attention: To meet the regulatory requirements of immunity from external electrical

STOP disturbances that may affect the performance of this instrument, do not use cables longerthan 9.8 feet (3 meters) when you make connections to the terminal strips on the rear panel of the unit. In addition, ensure you always connect the shield of the cable to chassis ground at one instrument only.

Caution: To protect against fire hazard, replace fuses with those of the same type and rating.

Caution: To avoid the possibility of electrical shock, power off the 2487 Detector and disconnect the power cord before you service the instrument.

Note: The Installation Category (Overvoltage Category) for this instrument is Level II. The Level II category pertains to equipment that receives its electrical power from a local level, such as an electrical wall outlet.

Symbols Used on Rear Panel of the 2487 Detector

Direct current

Alternating current

Protective conductor terminal Frame or chassis terminal Caution, risk of electrical shock (high voltage) Caution or refer to guide

Caution, hot surface or high temperature

2487 Detector InformationIntended UseYou can use the Waters 2487 Dual Absorbance Detector for in-vitro diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds. When developing methods, follow the Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory, American Journal of Medical Technology, 44, 1, pages 3037 (1978). This protocol covers good operating procedures and techniques necessary to validate system and method performance.

Biological HazardWhen analyzing physiological fluids, take all necessary precautions and treat all specimens as potentially infectious. Precautions are outlined in the CDC Guidelines on Specimen Handling, CDCNIH Manual, 1984.

CalibrationFollow acceptable methods of calibration with pure standards to calibrate methods. Use a minimum of five standards to generate the standard curve. Concentration range should cover the entire range of quality control samples, typical specimens, and atypical specimens.

Quality ControlRoutinely run three quality-control samples and monitor them daily. Quality-control samples should represent subnormal, normal, and above-normal levels of a compound. Ensure that quality-control sample results are within an acceptable range, and evaluate precision from day to day and run to run. Data collected when quality-control samples are out of specification may not be valid. Do not report the data until you ensure that chromatographic system performance is acceptable. Conduct a failure investigation to determine the cause(s) for the out-of-specification results.

Table of ContentsHow to Use This Guide..................................................................... 18 Chapter 1 2487 Detector Theory of Operation ................................................. 25 1.1 2487 Detector Description .................................................... 25 1.2 Principles of Operation.......................................................... 27 1.2.1 Waters 2487 Detector Optics..................................... 27 1.2.2 Wavelength Verification and Test............................... 29 1.2.3 Waters TaperSlit Flow Cell......................................... 30 1.2.4 Waters 2487 Dual Absorbance Detector Electronics ................................................................. 33 1.3 Operational Modes................................................................ 34 1.3.1 Single Wavelength Mode ........................................... 34 1.3.2 Dual Wavelength Mode ............................................. 35 1.3.3 Spectrum Scanning ................................................... 37 1.3.4 Cuvette Operations.................................................... 37 1.3.5 RatioPlot .................................................................... 38 1.3.6 MaxPlot...................................................................... 38 Chapter 2 Installing the 2487 Detector ............................................................. 39 2.1 Introduction ........................................................................... 39 2.2 Site Selection and Power Requirements .............................. 41 2.2.1 Site Selection............................................................. 41 2.2.2 Power Requirements ................................................. 42Table of Contents 6

2.3 Unpacking and Inspecting..................................................... 42 2.3.1 Unpacking.................................................................. 43 2.3.2 Inspecting .................................................................. 43 2.4 Making Fluidic Connections .................................................. 44 2.5 Making Electrical Power Connections................................... 47 2.6 Making Signal Connections .................................................. 49 2.6.1 Making I/O Signal Connections ................................. 50 2.6.2 Connecting the 2487 Detector to a 2690 Separations Module in a Stand-Alone Configuration..................... 52 2.6.3 Making IEEE-488 Connections in Breeze and Millennium ............................................................... 58 2.7 Connecting to Other Devices ................................................ 64 2.7.1 Connecting the 2487 Detector to the Millennium System Using the busSAT/IN Module ....................... 65 2.7.2 Connecting the 2487 Detector to a 745/745B/746 Data Module....................................................................... 68 2.7.3 Connecting the 2487 Detector to a Chart Recorder .. 71 2.7.4 Connecting the 2487 Detector to the Waters 600 Series Pump ......................................................................... 74 2.7.5 Connecting the 2487 Detector to the Waters 717plus Autosampler .............................................................. 79 2.7.6 Connecting the 2487 Detector to a Fraction Collector .................................................................... 81

Table of Contents

7

Chapter 3 Using the 2487 Detector .................................................................. 83 3.1 Starting Up the 2487 Detector .............................................. 83 3.1.1 Initializing the 2487 Detector ..................................... 83 3.1.2 Diagnostics Failure .................................................... 85 3.2 Using the Operator Interface................................................. 85 3.2.1 Using the Display....................................................... 85 3.2.2 Using the Keypad ...................................................... 88 3.2.3 Navigating the User Interface .................................... 93 3.2.4 Primary and Secondary Functions............................. 95 3.2.5 Operating the Trace and Scale Functions ............... 102 3.2.6 Operating Other Detector Functions........................ 104 3.3 Operating the 2487 Detector............................................... 109 3.3.1 Overview of Detector Operation .............................. 109 3.3.2 Verifying the Detector ...............................................110 3.3.3 Wavelength Calibration.............................................113 3.3.4 Operating the Detector in Single Wavelength Mode ........................................................................114 3.3.5 Operating the Detector in Dual Wavelength Mode ........................................................................115 3.3.6 Programming Timed Events, Threshold Events, and Methods....................................................................118 3.4 Scanning Spectra................................................................ 126 3.4.1 Scanning