STLCC_CPLS;Morrison 4/7/2014 Page 1

Hot Plates, Block Heaters,

Plate Incubator,

Water Baths, Bead Bath

Standard Operating Procedures

Prepared by: Bob Morrison

STLCC, Instrumentation Specialist

Mar08, Last revision Mar 11

Typical Digi-Block Jr.

@FV

Precision Analog

Water Bath@FV

Hot Plate &

Stirrer@FV

Precision Digital

Shaker @FV

BioRad-IPS Microplate

Heater/Incubator@FV Bead Bath

@ FV

Block Heater

@BRDG, Digital

VWR 18LShaker

Digital @ BRDG

VWR 18L Analog

Non-shaker

@ BRDG

Heater; Hot Plate, Stirrer

STLCC_CPLS;Morrison 4/7/2014 Page 2

Corning PC-420

Temp. Range: 25-550°C

Stir Range: 60-1100

Size: 5" x 7" Pyroceram© top

Corning PC-351

6"x7.5" ceramic top

• Stirring range: 250 - 1000 rpm

• Temperature range: 25 - 520°C

Corning PC-35

Temp. Range: 25-500°C

Size: 5" x 7”

Analog Stir on/off and

rpm Control Analog On/off and Heat

Control

“On” indicator lights

Heater: Dry Block, Digi-block Jr.

STLCC_CPLS;Morrison 4/7/2014 Page 3

On/Off

Use Up/Down Arrows

to set desired temp. Actual Block temperature

from probe

Probe: Insert in small

diameter hole in block

to monitor actual

temperature, displayed

above set value.

Error Conditions:

Er 2 – probe not working

Er 7 – no probe connected

Recommended: Insert

100C thermometer in

one hole/cell to

validate temperature

and probe display.

STLCC_CPLS;Morrison 4/7/2014 Page 4

Dry Block Incubator/Heater

Low and High Temperature Control Settings

(data sources: tech specs corrected by measured FVCC data (Tolerance: +5C, -10C)

0

20

40

60

80

100

120

140

160

Low Temp High Temp

Low Temp 22 22 22 22 22 25 30 35 40 50 60

High Temp 50 55 60 70 80 90 100 110 120 130 135

0 1 2 3 4 5 6 7 8 9 10

Low

Temp

Setting

High

Temp

Setting

On/Off

Still

Heating if

on

Recommended Procedure:

- Use the chart below to get initial settings for either the low or high dials

Note: they are not additive, use only one dial with the other set a “0”

- Add a thermometer to an open well hole and monitor the temperature

- Well should reach desired temperature in <10 min. for most models

- Adjust setting dial as needed to get closer to desired temperature

Heater: Dry Block, Digital, BRDG, Labnet, Dual block

STLCC_CPLS;Morrison 4/7/2014 Page 5

Product Specifications , Model D1200 (dual block) Temperature range Ambient +5º to 150ºC

Temp. resolution 0.1ºC

Temp. uniformity ±0.2ºC

Temp. accuracy ±0.3ºC

Block chamber Stainless steel

Block capacity

Single block unit One standard block

Dual block unit Two standard blocks or one dual block

Dimensions (WxDxH) 7.9 x 10.4 x 3.3 in./20 x 26.5 x 8.3 cm

Weight 5.5 lb/2.2 kg

Electrical 120V~, 50/60 Hz (also available in 230V)

Dry Bath Blocks

Construction High grade, nonporous aluminum with anodized

surface

1) Use Up/Down arrows to set desired temperature

2) Press Start/Stop to begin heating cycle, note green light

3) Press Start/Stop to turn off heating, light now off

4) To change temperature settings, Press

START/STOP once, use Up/Down arrows to enter

the new set point, then press START to resume

the new heating cycle.

5) Remain cautious of block after heat is turned off. 6) Toggle to OFF using switch in rear

On/Off toggle in Rear.

(4) Blocks for 24 x 0.2ml PCR Tubes

(4) Blocks for 24 x 1.5ml Tubes

(4) Blocks for 12 x 15ml Tubes

(4) Blocks for 5 x 50ml Tubes

D1102

D1105A

D1115-TALL

D1150-TALL

Baths: VWR, 18L, Shaking, Digital

STLCC_CPLS;Morrison 4/7/2014 Page 6

Temperature Range: 5 to 99°C (41 to 210°F)

Shaking Speed Range: 40–400 strokes/min.

Stroke Length: 18 mm Electrical: 115V, 50/60Hz

18.4L ,50.5 x 30 x 15 cm, VWR Cat # 89032-226

On/Off

toggle

(rear

Lower)

Themo

Protect

Set @ high

safe value

Setting Desired Temperature:

1. Turn the sample protection thermostat knob to maximum

2. Press Left arrow control key until “C” appears, press again to show current set temperature

3. Press ^UP or Down arrows to set the desired temperature

4. Press Left arrow control key again to store this set value

5. Reset the sample protection thermostat knob to a safe value

6. Temperature will revert to actual water temperature and move toward set value

7. Use a thermometer to verify that set temperature is accurate

Setting Shaking Speed:

1. Press Left arrow control key until “C” appears

2. Press the ^UP arrow until the display changes to a moving “[___]” symbol

3. Press Left arrow control key again to show the current shaking speed

4. Press UP arrow to set the desired speed between 40-400 or DOWN arrow to set to 0/zero

5. Press Left arrow control Key to lock in the desired speed

SPC-Single Point Calibration:

1. Enter this mode to finely tune the deviation from the set temperature to a closure tolerance.

2. Press Left arrow control key until “C” appears, then UP arrow until SPC displays

3. Use Up/Down arrows to set new tolerance, then Left arrow control key to save

Hotlink to VWR Water Bath User Manual …pdf

Baths: VWR, 18L, Analog, non-shaking

STLCC_CPLS;Morrison 4/7/2014 Page 7

Analog Water Bath 18 L

50.5L x 30W x 15D cm (1929/32 x 1113/16 x 529/32")

CAT# 89032-204

On/Off

toggle

(rear

Lower)

Themo

Protect

Set @ high

safe value

Hotlink to VWR Water Bath User Manual …pdf

Baths: Fuses, 12-15A, 250V required for most bath

STLCC_CPLS;Morrison 4/7/2014 Page 8

STLCC_CPLS;Morrison 4/7/2014 Page 9

STLCC_CPLS;Morrison 4/7/2014 Page 10

Water Bath: Precision Model 185 (static)

Specifications:

Volume 18 liters, chamber size 11.5"x19.5"x6"

Max temperature 99.9°C (65 deg;C without a cover)

Temperature uniformity 0.2°C at 37°C, sensitivity 0.1°C at 37°C

Operating voltage 120V

Desired Bath

Temp Setting

5 ~40C

Max High

Temp Setting

On/Off

Insert Thermometer through ring into

water to get actual temperature of bath

Safety Notes:

1) Make sure that the thermocouple probe in the

bottom of the tank is under the snap lever

and in contact with the tank bottom.

2) Turn off when not in use.

STLCC_CPLS;Morrison 4/7/2014 Page 11

Water Bath: Reciprocal Shaking - Precision Digital

Microprocessor control with digital LED display and shaker control. Push button convenience for entry of

temperature and shaking speed, Over-temperature protection capability, Automatic motor shut down

capability, One-piece, easy-to-remove tray for easy use and maintenance. Bath provides the gentle,

reciprocating motion required for many common biological, tissue culture, and biotechnology

applications. Suitable for use in applications which require a controlled atmosphere.

Chamber Capacity 3.9 gallons / 14.5 liters

Maximum Temperature 99.9oC

Temperature Display

Temperature Uniformity ±0.05oC at 37oC

Temperature Sensitivity ±0.05oC

Oscillation per Minute 30 to 200

Stroke Length 0.5 to 1.5 in.

Bath: Bead, Lab Companion (Jeiotech), 20L

STLCC_CPLS;Morrison 4/7/2014 Page 12

Operating features for BW-20B (Analog model)

• 7℃ above room temperature to 100℃

• PID temperature control

• Over-temperature limiter, alarm indication

• Analog dialed indication with fine adjustment

Dimensions: 19.6” x 11.8 x 6.5 (bathsize)

22.2” x 15.4 x 11.6 (cabinet) 18.5lbs without beads

Constructional features

• Low-profile baths with front slope-panel controls provide easier access and safe operation

• Additional inner circulating system by equipped agitator (BW-E/ D series)

• Dual bath models features individually temperature controller chambers (BW-D series)

• Various accessories gable and flat covers, test tube and spring wire racks, half-shelf adjusters etc. (optional)

* All accessories for dual baths (BW-D series) refer to BW-E/ B series accessories options per each bath size.

(Ex. Each of gable covers for BW-1005D are same as one cover for BW-05E/ B and the other cover for BW-10E/ B)

Dry, metallic beads that replace water in water baths, aluminum

blocks in dry baths, or ice in ice buckets. Bath Armor™ Beads

are convenient, versatile, and can maintain hot or cold sample

temperatures in just about any vessel type.

MIDSCI.com

42370-016 Bath Armor

Beads 16L $900.00 Set Temp

Maximum

(screwdriver) Set Time

For Bath

On/Off

Toggle and

Fuses

Specifications:

Operating range 30-40°C

Temperature variations ±0.5°C

Dimensions 240 x 360 x 160 cm (9,4 x 14,1 x 6,2 in)

Weight 7.2 kg (15,8 lb)

Capacity 1 to 4 x 96-well micro plates

Heater: Plate Incubator; BioRad-IPS, Digital, Type B

STLCC_CPLS;Morrison 4/7/2014 Page 13

The IPS microplate incubator is a dry incubator

designed to heat and maintain a constant and

homogeneous temperature inside the wells of a

microplate.

• Usable for all microplate techniques

• No water or maintenance required

• Incubation temperature regulated to within 0.5°C

• Four plate pads

Note: Allow 5 minutes to reach set

temperature of 37C degrees. 1. On/Off toggle in rear

near voltage plug,

2. Red light “on”

indicator

4. Push this button to get

a current “read” of the

plate temperature

3. Displays approximate

temperature of plates,

use “read” button to

update.

Calibration “screw”, do

not tamper with unless

you have accurate

devices to measure plate

temperatures inside.

110 or 220V set screw on

rear panel. Leave at

110V position.