Essential innovations from VWR SSUE OVEMBER vwr

vwr.comEssential innovations from VWR ISSUE 7 - NOVEMBER 2008

TitroLine KF trace - coulometric Karl Fischertitration from Schott

page 8

Additions to the Stuartmelting point range

page 10Are you registered for our e-newsletter? Get all the latest news and offers go to www.vwr.com

New microscopes from the VWR Collection plus complete rangeof cold light sources

page 5

Julabo FL Series Chillers environmentally friendly and economic cooling

page 6

table of contents

editorial

From air monitoring to water testing, stability testing or recycling of

electrical items to meet the WEEE directive this issue has something

for every industry or application.

There are features on high end investment products such as spectro-

photometers, centrifuges and balances. As well as the time saving

rotary evaporators and the safety and security of a good fume hood.

We're also pleased to announce the formation of the VWR BioSciences

group to support Life Scientists with cutting edge instrumentation.

Finally ... Do you Sudoku? Try our brainteasing competition on the back

page and you could win one of our great prizes.

Essential innovations from VWR

EditorVWR International Europe bvbaHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International Europe bvba

Layout and typesettingMarketing Services VWR

PrintingStork Druckerei GmbH, Bruchsal/Germany No part of this publication may be reproduced or copied withoutprior permission by writing of VWR International Europe.

Run177.000 copiesPublication date: November 2008

Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.

table of contents

editorial

Unbelievably the year is nearly over, but before 2009 arrives we have some

great equipment choices for your laboratory.

The fantastically popular VWR Collection microscope range increases with

new phase contract, polarisation and digital models.

Schott showcase the Titroline KF trace for coulometric Karl Fischer and

Stuart launch their new melting point range that saves you from having to

wait around.

Plus we have our first entry from Huber, specialists in ultra low tempera-

ture work as well as some great product features for all applications.

Seasons greetings!

See you again next year!

Best wishes

Your VWR equip team


The VWR Collection dry block heater 3

Illumination systems, new from the VWR Collection 4

New VWR Collection microscopes 5

Julabo FL Series chillers-environmentally friendly and economic cooling 6

Huber presents a new generation of controllers 7

TitroLine KF trace - coulometric Karl Fischer titration from Schott 8

Food safety – use of incubators in food microbiology with Binder 9

Additions to the Stuart melting point range 10

Eppendorf's new Biophotometer plus 11

SCAT Europe solvent level control is now available for all laboratories 12

Personnel safety and reliable weighing results, from Sartorius 13

VWR Collection, Uncertainty Manager® 14

VWR E q u i p M a g a z i n e Issue 7 N o v e m b e r 2 0 0 82

Competition!

Do you like a puzzle?Test yourself against the clock andtry your hand at our competitionon www.vwr.comand you could win An Eternityemergency rechargeableflashlight. 15-30 seconds ofshaking offers up to 5 minutes ofcontinuous bright LED light thatis visible up to 1 mile away. Thewaterproof case even floats.

Simply put all the pieces of thepuzzle together to make our special VWR equip picture - the fastest 10 entries will win.

Once you have solved the puzzleclick on the send button and fill in your name and email address to be entered into our prize draw.

Closing date for entries31/12/2008

• Exceptional temperature uniformity and stability

• Optional external temperature probe

• Heat from ambient +5 °C to 120 °C

• LED display

VWR E q u i p M a g a z i n e Issue 7 N o v e m b e r 2 0 0 8 3

Essential equipmentFor more information on these products complete the faxback enquiry form on the back

cover and send to your local VWR sales office orvisit our website www.vwr.com and click on equip.

VWR digital dry block heaters

Micro-processor PID control, with optional external probe, offers a temperature stabilityand uniformity of ±0,2 °C.

Easy to use controls with independent LED displays for temperature and time. Controls allow users to adjust temperature setpoint in ±0,1 °C increments.

Timer will display elapsed time or when programmed to user defined limit, will count downto zero and shut off unit.

Audible alarm will sound when time reaches zero.

Optional external temperature probe kit monitors actual block or sample temperature.LED display on front panel will show actual probe temperature and adjust heater output tomaintain user defined set-point. All dry block heaters include a built-in support rod holder formounting the probe kit. Kit includes stainless steel RTD probe, 457 mm vertical support rod,thermometer/probe extension clamp and hook connector.

Calibration mode allows operator to adjust temperature display to match an externalthermometer. When measuring your dry block heater it is recommended that you use either aglass thermometer inserted into the thermowell or an electronic surface temperature probe.Modular blocks are sold separately. Heaters will accept blocks from older VWR units.

Temperature stability is defined as the fluctuations in temperature once the unit hasreached set point and cycles the heater on and off to maintain that set-point. This ismeasured in one single thermometer well in the block.

Uniformity is defined as the difference in temperature from one end of the block to the otheror from one block to another block of the same type.

Modular Heating Blocks are interchangeable and accommodate various tube sizes from0,2 ml microtubes to 50 ml. Each block has a thermometer well for measuring blocktemperature. Efficient heater features close tube and block contact with a low density designfor exceptional temperature uniformity. Our most popular blocks are 460-3212 for 1,5 mlEppendorf tubes, 460-3216 for 13 mm test tubes, and 460-3221 for 15 ml centrifuge tubes.See www.vwr.com for over 40 block options.

Designed for applications that require repeatable results and superiortemperature stability. These multi-purpose units are ideal for incubation and activation of cultures, enzyme reactions, immunoassays, melting/boilingpoints, and a wide variety of other laboratory procedures.

Buy a 1 or 2 block Digital

Dry Block Heater get a FREE

Temperature ProbeKit use special

catalogue numbersbelow*

Blocks W x D x H Electrical Weight Cat. No. Cat. No. Cat. No.(mm) (50/60 Hz) - (230V) (kg) EU plug UK plug CH plug

1** 203x315x89 0,5 amps 110 watts 2,6 999-3264 999-3265 999-3266 2** 203x391x89 0,9 amps 210 watts 2,9 999-3267 999-3268 999-32693 203x467x89 0,9 amps 210 watts 3,6 460-3270 460-3271 460-32724 203x429x89 1,3 amps 310 watts 3,9 460-3273 460-3274 460-32756 203x531x89 1,7 amps 410 watts 4,5 460-3276 460-3277 460-3278

Description Cat. No. Cat. No. Cat. No.Euro plug UK plug CH plug

Optional External Temperature Probe Kit 460-3288 460-3268 460-3269

Performance data was calculated using an external temperature probe inserted into the thermowell.

* Offer valid until 31/12/08 **Special package Cat. Nos.

GreatValue

Number Temperature Stability Uniformity Uniformity Stability Uniformity Uniformity Heat-up time of range @ 37 °C within the block across Similar @ 60 °C within the across similar to 100 °Cblocks (°C) (°C) @ 37 °C (°C) blocks@ 37 °C (°C) (°C) block @ 60 °C (°C) blocks @ 60 °C (°C) (min)

1 N/A N/A 452 Ambient ± 0.3 ± 0,5 503 +5 to 120 ± 0,2 ± 0,2 ± 0,4 ± 0,4 ± 0,4 ± 0,7 554 ± 0,5 ± 0,8 606 ± 0,6 ± 1,0 65


VWR Collection Visilight illumination systems


New products

Halogen Cold Light Sources VisiLight CL 30 and CL 150

The VWR VisiLight cold light sources, with a modern and attractive design guarantee a preciseobject illumination in microscopy. The optimal thermal management maintains a low temperatureand protects the light guides and the electronics. Selectable input voltages of 88-264 V.AC enablea flexible and international use. The integral soft start protects the lamp and prolongs its life span.• User friendly and stackable, vertical or horizontal positioning• Continuous electronic dimming from 0 to 100% for optimal application• Noiseless and vibration-free operation• Practical filter holder (CL 150 only) for a range of optional filters • Aluminum housing

Ordering information: Goose neck light guides, focus optics, ring light and filters must beordered separately

Fiber Optic Ring LightRing lights with 360° lighting, enable shadow-free illumination of the object in the area of microscopy andphotography.They are available with a standard inner Ø of 66 mm and a standard length of 750 mm.The ring light can be fitted to microscopes and other instruments using special ring adapters.

VWR LED Illumination

LED spot lightLED lamps from the VisiLight I Led-series are designed for use in clinical and industrial research.LED technology makes the product a very efficient alternative to current halogen lamps and fiberoptic systems.• The 3 Watt power LEDs have an average life span of 50,000 working hours• Colour temperature is typically 5500 K, quite similar to daylight, for many professional applications• Continuous dimming driven by an analogue potentiometer• To be used with a holder or directly with the microscope

LED Ring Light, R LEDThe R LED has been developed for applications in stereomicroscopy. It delivers a shadow free,flicker free and uniform lighting for your application and is suitable for all types of camera ormicroscope. The ring light can be adapted from Ø 66 mm to Ø 50 mm. The ring light consists of 48 long lasting, white LEDs. As an optional accessory a diffuser delivers scattered light for specialapplications.• Optimal solution for applications requesting uniform illumination• Diffuser minimising mirror effects and designed for quality control and inspection• Plug and Play

Description Cat. No. Cat. No.EU plug UK plug

I-Led 1, 1 arm 631-1754 631-1773I Led 2, 2 arm 631-1755 631-1774Microscope stand for LED Lamp I Led 1 631-1770Holder for stereo-microscope stand Ø25mm 631-1776Holder for stereo-microscope stand Ø32mm 631-1777Wall holder for 631-1778stereomicroscopeLED Ring 631-1756 631-1775Light R LED (Ø 66 mm)Frosted glass diffuser 631-1757

Description Cat. No.

Fibre Optic ring light (Ø 66 mm, 750 mm, Ø 8 mm) 631-1753Ring light adapter Ø 50 mm 631-1779

Description Cat. No. Cat. No. EU plug UK plug

VisiLight model CL 30, 30 W 631-1750 631-1771VisiLight model CL 150, 150 W 631-1751 631-1772Gooseneck light guide (2 arms, 450 mm, Ø 4,5 mm) 631-1752Focus optic for Gooseneck light guides which enables an 631-1763optimal guiding of the light beam for individual applications.

Model CL 30 CL 150

Intensity 30 W 150 WFilter No YesLighting Electrical Electrical dimming + Iris diaphragmWeight 2,9 kg 3,5 kgW x D x H (mm) 244x164x132 294x164x132


For more information on these products complete the faxback enquiry form on the backcover and send to your local VWR sales office orvisit our website www.vwr.com and click on equip.Essential equipment

Description Type Cat. No. EU plug Cat. No. UK plug Cat. No. CH plug

BI 500 PH Binocular 630-0895 630-0990 630-0997

TR 500 PH Trinocular 630-0896 630-0991 630-0998


BI 500 PL Binocular 630-1097 630-1098 630-1099

TR 500 PL Trinocular 630-0988 630-0995 630-1002


DS 100 Monocular 630-1109 630-1110 630-1111

BI 500 PH and TR 500 PH New phase contrast microscopes

Type Binocular or TrinocularContrasting technique Phase contrastObjectives Plan achromatic 4x, plan achromatic phase contrast, 10x, 40x (spring),

100x (oil, spring)Eyepieces Wide field 10x/20 with centre scopeHead 30° inclined and 360° rotatingNosepiece 5 position nosepiece (backward)Stage Built in coaxial mechanical stage 210 x 140 mm, travel 75 x 50 mm,

coarse and fine focusing adjustmentCondenser 1,25 N.A. phase contrast condenser, centre adjustableIllumination Halogen 6V 30W Koehler illumination

BI 500 PL and TR 500 PL Series: Polarisation microscopes

Type Binocular or TrinocularOptical Bright field or infinityObjectives Strain-free plan achromatic 4x, 10x, 40x (spring),100x (spring, oil)Eyepieces Wide field 10x/18 mmHead 30° inclined Nosepiece 5 position nosepieceStage Round stage 150 mm diameter, 360° rotateCompensator λ,λ/4, Quarts wedgeBertrand Lens Plug in, centre adjustableAnalyser 360° rotate with scalePolariser 360° rotate with scaleCondenser 1,25 N.A. polarise condenser, iris diaphragmIllumination 6V 30W halogen lamp, adjustable brightness

DS 100, Digital Monocular Microscope with built in camera

Type Monocular Objectives Achromatic 4x, 10x, 40x, 100x (oil)Eyepieces Wide field 10x/18 with pointerHead 45° inclined and 360° rotatingNosepiece 4 position nosepieceStage Coaxial mechanical stage 120 x 120 mm, coarse and fine focusing adjustmentCondenser 1,25 N.A. Abbe condenser, iris diaphragm with carry filter (blue filter)Illumination LED illumination with batteryCamera Integrated 1,3M pixel camera, maximum resolution 1280 x 1024,

USB 2,0, driver CDROM included

Ergonomic designprovides a comfortableworking position

New VWR Collection microscopesFirst for choice and valueNew

products

>



JULABO presents the FL Series, the latest generation of chillers for coolingapplications in laboratories, pilot or miniplants and throughout industry. There are 20different models with a wide variety of features; a choice of air or water cooled unitsoffers the ideal solution for a range of applications such as accompanying rotaryevaporators, distillation apparatus, mills or kneaders. Other applications include use inthe semiconductor or plastics industries, cooling autoclaves, reaction vessels, Soxhletinstallations, vacuum systems, gas chromatographs, spectrophotometers, Lasers,diffusion pumps, mass spectrometers and electron microscopes.

The FL series includes compact chillers which can be placed under or on top of the labbench - as well as more powerful models up to 11 kW cooling capacity with smallfootprints. All units have stainless steel bath tanks, a bright LED temperature displayintegrated into a splash proof keypad for easy cleaning. An RS232 interface as well asan analogue alarm output are located on the front panel, the lift-up cover at the topof the unit gives convenient access to the filling port and the front drain assists withemptying. The removable venting grid means the condenser can be easily cleaned. Allmodels include an easy to see level indicator. Venting slots are exclusively located onthe front and back, allowing units to be placed directly next to each other.

The 'FL Series': 20 models in different performance categories

Air cooled models (IP class according to IEC 529: IP21)

FL Series chillers environmentally friendly and economic cooling FL300, FL601, FL1703, FLW4006, FL11006

• Working temperature range from -20 °C to +40 °C with cooling capacities up to 11 kW

• PID temperature control with stability of ±0,5 °C• Easy filling from the top with protective hinged lid

and filling level indicator• Powerful immersion pumps, suitable for continuous

operation with capacities up to 60 l/min flow rate and 6 bar pressure

• Permissible temperature in return line +80 °C• Low liquid level protection with optical and

audible alarm signal• Pressure indicator and adjustable bypass for

pump pressure (models with pressure >1 bar)

Model Temperature Temp. Cooling Filling W x D x H Cat. No.Range Stability capacity volume (mm)(°C) (°C) (kW)* (l)

FL300 -20 to +40 ±0,5 0,3; 0,2; 0,15 3...4,5 250 x 500 x 600 461-3465FL601 -20 to +40 ±0,5 0,6; 0,4; 0,33 5,5...8 320 x 500 x 600 461-3466 FL1201 -20 to +40 ±0,5 1,2; 0,9; 0,6 12...17 500 x 760 x 640 461-3467 FL1203 -20 to +40 ±0,5 1,2; 0,8; 0,5 12...17 500 x 760 x 640 461-3469 FL1701 -20 to +40 ±0,5 1,7; 1,1; 0,85 12...17 500 x 760 x 640 461-3468 FL1703 -20 to +40 ±0,5 1,7; 1,0; 0,75 12...17 500 x 760 x 640 461-3470 FL2503 -20 to +40 ±0,5 2,5; 1,5; 1,2 24...30 600 x 760 x 1150 461-3471 FL2506 -20 to +40 ±0,5 2,5; 1,0; 0,3 24...30 600 x 760 x 1150 461-3473 FL4003 -20 to +40 ±0,5 4,0; 2,4; 1,5 24...30 600 x 760 x 1150 461-3472 FL4006 -20 to +40 ±0,5 4,0; 1,9; 0,9 24...30 600 x 760 x 1150 461-3474 FL7006 -20 to +40 ±0,5 7,0; 5,5; 3,0 39...47 780 x 850 x 1480 461-3475 FL11006 -20 to +40 ±0,5 11,0; 7,5; 5,0 39...47 780 x 850 x 1480 461-3476

* Cooling capacity at 20; 0; -10 °C

Model Temperature Temp. Cooling Filling W x D x H Cat. No.Range Stability capacity volume (mm)(°C) (°C) (kW)* (l)

FLW1701 -20 to +40 ±0,5 1,7; 1,1; 0,85 12...17 500 x 760 x 640 461-3477 FLW1703 -20 to +40 ±0,5 1,7; 1,0; 0,75 12...17 500 x 760 x 640 461-3478 FLW2503 -20 to +40 ±0,5 2,7; 1,7; 1,3 24...30 600 x 760 x 1150 461-3479 FLW2506 -20 to +40 ±0,5 2,5; 1,0; 0,3 24...30 600 x 760 x 1150 461-3481 FLW4003 -20 to +40 ±0,5 4,3; 2,5; 1,6 24...30 600 x 760 x 1150 461-3480 FLW4006 -20 to +40 ±0,5 4,0; 1,9; 0,9 24...30 600 x 760 x 1150 461-3482 FLW7006 -20 to +40 ±0,5 7,4; 6,0; 3,1 39...47 780 x 850 x 1480 461-3483 FLW11006 -20 to +40 ±0,5 11,5; 8,0; 5,1 39...47 780 x 850 x 1480 461-3484

* Cooling capacity at 20; 0; -10 °C

Water cooled models (IP class according to IEC 529: IP21)



A new generation of controllers

The new generation of controllers used on the full range of Huber instruments gives the user aneasily accessible array of features that, through “E-Grade” technology, can be upgraded to meetevolving demands at any time. Features include a choice of the way data is displayed fromgraphically to numerically, large or small characters that show every important parameter at aglance, RS232 fitted as standard, unique safety features including Huber’s “Process Safety OverTemperature”, early warning systems, powerful & adjustable pressure & suction pump (vpc) andmuch more. The robust & compact stainless steel construction houses these features in a durableframe to withstand day to day use & abuse for many years. This new range is simply unbeatable inpracticality and easy to use features.

Compatible Control Cooling BathsThe K6 models are compact refrigeration bath circulators for temperatures from -25 to 200°C.These units are a combination of a miniature refrigerated bath and immersion circulator CC1, CC2or CC3, in combination with a pump adapter they are suitable for external applications. Thecombination with the immersion circulator CC-E with its suction/pressure pump is suitable forexternally open and closed applications. The temperature stability is better than 0,02 K to DIN12876. The K6 models and the powerful K6s-CC are low cost alternatives to the Ministat 125,the smallest refrigeration circulator in the world and bestseller since 1976.

K6-cc and K6s-cc

CC-505

Ministat Range

Model Working Heating Bath Pump Data Cooling Power (kW) Temp. Power Volume Depth Max. Pressure. Max. Suction at (°C)Range (kW) (l) (mm) (l/min) (bar) (l/min) (bar) 100 20 0 -20 -40

CC-505 -50..200 1,5 5 150 33 0,7 22 0,4 1,2 1,2 1,0 0,6 0,15CC-505wl -50..200 1,5 5 150 33 0,7 22 0,4 1,2 1,2 1,0 0,6 0,15CC-510 -50..100 3,0 18/11* 200 31 0,6 24 0,35 2,1 2,1 2,1 1,0 0,4CC-510w -50..100 3,0 18/11* 200 31 0,6 24 0,35 2,4 2,4 2,4 1,0 0,4CC-515 -55..100 3,0 26/15* 200 31 0,6 24 0,35 3,3 3,3 3,3 1,6 0,6CC-515w -55..100 3,0 18/11* 200 31 0,6 24 0,35 3,3 3,3 3,3 1,6 0,6CC-520w -55..100 3,0 17/10* 200 31 0,6 24 0,35 5,0 5,0 5,0 3,0 1,5CC-525w -55..100 3,0 17/10* 200 31 0,6 24 0,35 7,0 7,0 5,0 3,0 1,5

* with displacement insert

Model Working Heating Bath Pump Data Cooling Power (kW)Temp. Power Volume Depth Max. Pressure Max. Suction at (°C)Range (kW) (l) (mm) (l/min) (bar) (l/min) (bar) 20 0 -20

Ministat 125-cc -25..150 1,0 2,75/1,3* 120 27 0,7 20 0,4 0,30 0,21 0,05Ministat 125w-cc -25..150 1,0 2,75/1,3* 120 27 0,7 20 0,4 0,30 0,20 0,10Ministat 230-cc -33..200 2,0 3,2/1,7* 135 27 0,7 20 0,4 0,40 0,35 0,25Ministat 230w-cc -33..200 2,0 3,2/1,7* 135 27 0,7 20 0,4 0,40 0,35 0,25Ministat 240-cc -40..200 2,0 4,9/2,8* 157 27 0,7 20 0,4 0,55 0,50 0,35Ministat 240w-cc -40..200 2,0 4,9/2,8* 157 27 0,7 20 0,4 0,55 0,50 0,35

Model Working Temp. Heating Bath Pump Data Cooling Power (kW)Dimensions Range Power Opening Depth Vol. Max. Pressure Max.Suction at (°C)

(kW) (mm) (mm) (l/min) (bar) (l/min) (bar) 0 -10 -20

CC1-K6 210x370x535 -25..200 2 140x140 150 4,5 10 0,2 - - 0,15 0,1 0,05CC2-K6 210x370x535 -25..200 2 140x140 150 4,5 10 0,2 - - 0,15 0,1 0,05CC3-K6 210x370x535 -25..200 2 140x140 150 4,5 10 0,2 - - 0,15 0,1 0,05K6-cc 210x370x546 -25..200 2 140x120 150 4,5 27 0,7 25 0,4 0,15 0,1 0,05K6s-cc 250x370x576 -25..200 2 140x120 150 4,5 27 0,7 25 0,4 0,21 0,15 0,05

For ordering details please contact your local VWR sales office



TitroLine KF traceCoulometric Karl Fischer titration made easy,with simple dialogue

Coulometric water determination according to Karl Fischer is straightforward and precise with thenew TitroLine KF trace. The large display prompts the user through a menu driven dialoguestructure. The pre-parameterised methods are easily recalled and speed up the entire workingprocess. The versatility of the KF titrator provides a trouble-free KF measuring device for nearly all areas of industry, such as the pharmaceutical, chemical and petroleum industries.

The coulometric Karl Fischer titrator TitroLine KF trace is a dedicated instrument for determiningvery low and trace levels of water content in your samples. As the coulometric determination ofwater does not require the standardisation of a titrant, the operation is easier compared withvolumetric titration methods: Once the instrument is installed, the reagents are inserted into thetitration cell and the instrument is switched-on. The TitroLine KF trace starts to operateimmediately. The conditioning is triggered in the background and automatically determines thedrift. Within a few minutes the TitroLine KF trace is ready for testing the first samples.

TitroLine KF traceMeasuring range 10 μg-100 mg / 1 ppm 5% (recommended)Methods 10 (9 x sample, 1 x blank) Conditioning Automatic after switch onEndpoint criteria Drift, drift toleranceAuto start after Yessample additionStatistics Average, sd, rsdRecalculation YesOn-line curve YesDocumentation GLP + curve print outResult printout %, ppm, mg, mg/l, mg/pc, μgPassword protection YesSoftware update Update via RS232 and USB


TitroLine KF trace, module 1 661-0088TitroLine KF trace, module 2 661-0089TitroLine KF trace, module 3 662-1960TitroLine KF trace, module 4 661-0090

Convenient - with preset methodsAll methods are already preset with the most commonly used parameters, butcan be edited if required. A choice of dialogue in 4 languages includes English,French German and Spanish although other languages may be available on request.

Live titration processThe TitroLine KF trace enables you to monitor progress of the titration by displayinga real-time measuring curve. With just a keystroke there is the option to switchbetween the standard or graphic display.

You can decide whether you would like to print the results in either a short form, asstandard form with a curve or as a complete GLP printout, including all methodparameters. Of course all results are displayed with average value and drift.

TitroLine KF trace modules: Module 1 includes TitroLine KF trace basic instrument, magnetic stirrer, full body

glass titration vessel, micro double platinum electrode with fixed cable,connecting cable and generating electrode TZ1752 without diaphragm.

Module 2 is the same as module 1 but includes a KF filtration stand with pump and titration vessel instead of the magnetic stirrer and full body glasstitration vessel.

Module 3 is the same as module 1 with the generating electrode TZ 1753 withdiaphragm instead of the TZ 1752 without diaphragm.

Module 4 is the same as module 2 with the generating electrode TZ 1753 withdiaphragm instead of the TZ 1752 without diaphragm



Optimum growth parameters in microbiological incubators

Microorganisms grow in diverse biospheres and have different optimal temperature and growthconditions. Bacteria multiply only within a narrow temperature tolerance band, for example. Therequirements for an incubator used in quality control are thus correspondingly high. A high-qualityincubator should have the following characteristic

• High temperature accuracy and consistency• Fast recovery times after the door is opened • Low sample dehydration • Long-term reproducibility of the data obtained

Food safety – use of incubators in food microbiology

According to estimates by the World Health Organization (WHO), approximately 10% of thepopulation in the industrialized nations become ill due to contaminated food. In Germany alone,roughly 200,000 infections per year due to contaminated food are recorded, but in reality, this figureshould be multiplied by a factor of 10. This results in high costs due to lost working days andhealthcare measures. The WHO has developed a global strategy to reduce the health and socialburden, and increase food safety. In this connection, the WHO has also identified the new challengesfor the global food production industry, such as new process technology in the manufacture offoodstuffs and the transportation of food products across different climatic zones. Following the concept of the Farm to Fork approach, all partners in the food supply chain from the food& beverage manufacturers to the food retailers must play their part in creating the basis for safe food.

Model Inner W x D x H W x D x H Cat. No.Volume (l) ext. (mm) int. (mm)

KB 23 20 433 x 516 x 618 222 x 277 x 330 390-6076KB 53 53 634 x 576 x 837 400 x 330 x 400 390-6080KB 115 115 834 x 646 x 1022 600 x 400 x 480 390-6081KB 240 240 905 x 765 x 1458 650 x 470 x 785 390-6088KB 400 400 834 x 716 x 1850 650 x 470 x 1308 390-6083KB 720 700 1234 x 867 x 1816 1000 x 600 x 1168 390-6084

KB series: Refrigerated microbiological incubators with program controller, the all rounder, with individual programming functions, including a real-time clock function and a widetemperature range of – 10 to 100 °C.

The International Organisation for Standardisation (ISO) has developedstandards for the requirements on incubators for foodstuff and animal feed.

ISO-Standard Title of Standards Incubation temperature

4833:2003 (E) Horizontal method for bacterial count determinationof colonies at 30°C 30° ± 1°C

6888-3:2003 (E) Horizontal method for bacterial count determinationof coagulase-positive Staphylococcus aureus andother types 37° ± 1°C

7251:2005 (E) Horizontal method for the detection and microbial count of E. coli 37° ± 1°C; 44° ± 1°C

BINDER’s refrigerated microbiologicalincubators KB series provide the bestconditions for meeting these requirements:

• Exceptionally broad temperature range of -10 °C to 100 °C

• APT.line™ preheating chambertechnology with refrigeration system guarantees high temperatureaccuracy and reproducible results

• Microprocessor control with variousprogram and time functions

• Equipment qualification, validation and comprehensive options

New generation - BINDER KB 240 series, withimproved cooling technology



The use of melting point apparatus has been commonplace in the chemistrylab for many years, melting point is a quick and easy way to identify chemicalsor determine their purity. One of the largest suppliers of melting points,Stuart, has recently updated and extended its range, the former top of therange SMP3 is replaced by the SMP30 and for the first time the range hasbeen extended to include an automatic model the SMP40.

The SMP30 offers many features over its predecessor; the maximum temperature has been raisedto 400 °C, a cooling time of 10 minutes is easily achieved down to 50 °C thanks to the internalcooling fan.

One of the problems with melting point determination is that you are trying to keep your eye ontwo things at once, the melting chemical and the temperature of the block. The SMP30 overcomesthis problem by use of its innovative head up display. An image of the block temperature isdisplayed in front of the tubes, through the eyepiece. This allows users to keep an eye on theaction and the temperature at the same time.

The SMP40 utilises digital imagery to automatically, accurately identify the melting point. A videoof the melt is recorded during melting point determination to give greater traceability of the resultslong after the sample has gone. Melting point determination, whilst on the whole quick and easy,inevitably ties the operator to the unit whilst the melt is taking place. I’m sure that we have allmissed a melt or two along the way because we got distracted. Once the sample has been loaded,the SMP40 frees the operator up to do other things and you’ll never miss a melt again, and forcomplete piece of mind the video of the melt can be reviewed on the colour 5,7” VGA touchscreen display at any time to check that you agree with the units result.

The SMP40 is the first melting point apparatus to offer a split design concept. The control interfacecan be split from the block to allow the ultimate in footprint flexibility, once the sample is loadedthe block can be moved to the back of the bench, or even to a shelf to allow more working area.When split the control panel can be used in two orientations flat on the bench or upright to allowa comfortable viewing angle whether you are stood or seated at the bench.

This range of melting point apparatus, as with all Stuart equipment, comes as standard withBioCote antimicrobial protection at no extra cost and a three year warranty.

Additions to the Stuart melting point range

Model Description Max. Temp. (C°) Cat. No.

SMP11 Melting point apparatus, analogue 250 621-0036SMP10 Melting point apparatus, digital 1 °C resolution 300 621-2020SMP30 Melting point apparatus, digital 0,1 °C resolution 400 621-2022SMP40 Melting point apparatus, automatic 400 621-2023


For more information on these products complete the faxback enquiry form on the backcover and send to your local VWR sales office orvisit our website www.vwr.com and click on equip.Applications

The BioPhotometer plus, a compact UV/Vis photometer, is optimisedfor use in molecular biology, biochemistry and cell biology labs.

The photometer provides instant access to 32 routine methods,of which 9 methods are freely programmable. Measurements as well

as calculations of results are performed at the press of a button.The results of the analysis and all accompanying data are available at a glance.This guarantees safe and error-free operation. In addition to its low weight, the photometer is small, yet extremely sturdydue to a robust metal housing, which allows for easy transport and cleaning.

The BioPhotometer plus is designed for use with cuvettes such as the disposable EppendorfUVette®, but is also optimised for measurement of small volumes through the use of microlitre cells.

Features:• 9 wavelengths suitable for the common methods in molecular biological,

biochemical and cell biological labs• Preprogrammed methods for quantification of nucleic acids and proteins

as well as incorporation rate of fluorescent dyes• Assays at 340 nm, 405 nm, and 490 nm are user defined for highest flexibility• Automatic calculation of dilution factors• Measurement of single wavelengths without any calculation• Storage of the last 100 results and all corresponding data• Simple user guidance for error-free operation• Compact design and robust housing• No pre-warming required• Stand-alone unit without need for PC connection

Providing a cost-effective and reliable alternative for quartz cuvettes, the Eppendorf UVette is asingle-use, fully UV-transparent plastic cuvette for all common spectrophotometrical analyses inthe UV and visible range. The UVette is optimised for use with the Eppendorf BioPhotometer,however, with the help of an adapter it can be used in nearly every spectrophotometer.

Eppendorf offers two different qualities: Individually packaged UVettes, certified RNase-free,DNA- and protein-free, and the UVette routine pack, the alternative for everyday applicationsin the approved Eppendorf quality.

Features:• UV-/VIS-transparent between 220 nm and 1 600 nm• Small sample volume ± 50 μl• 10 mm and 2 mm optical light path• Clean room production

BioPhotometer plus and the disposable UVette a highly versatile package

New

For more informationplease contact your local

VWR Life Science specialist



Safe and comfortable monitoring

of liquid levels in the laboratory.

Electronic level control systems

from SCAT Europe are practical

and easy to install, no tools or

other equipment is needed.

SCAT Europe Systems are developed

especially for laboratory applications

and ensure trouble free workflows

without interruptions.

Touchless level controlLevel monitoring can be achieved without touching the fluids by using an adjustable sensor fixedto the outside of the container, at the appropriate fluid level. The critical fluid level is indicated byan acoustic signal and LED on the signal box. Control systems are suitable for all glass and plasticcontainers (non-conductive material only).

Watch up to 15 fluid levels at the same time with SCAT Europe signal boxes. If the critical fluidlevel is reached you can switch and control auxiliary equipment (pumps, valves, HPLC-systems etc.)by the integrated potential free relay contact

Level control for conductive PE-HDThis system has an integrated float and electronic sensor in the SCAT Europe Safety Waste Cap.Critical levels are indicated by the level float on the top of the cap as well as by the beeper andLED on the signal box. SCAT Europe Safety Waste Caps are available with all common threadsizes. The integrated exhaust filters avoid the escape of hazardous vapours and gases. Optionalwaste container can also be purchased.Also available as low-level warning for empty bottles and containers that helps avoid insufficientsolvent supply interrupting, for example, HPLC runs.

Finally- Level control is available for all laboratories


Sensor with signal box, power supply (110/230V), signal cable and mounting parts 590-0682Signal box (1 channel), with power supply 590-0726Signal box (5 channel), with power supply 590-0731Signal box (15 channel), with power supply 590-0738Extension cable for signal transmission, 3 m 590-0739Extension cable for signal transmission, 5 m 590-0727Extension cable for signal transmission, 10 m 590-0728Cable for relay contact 5 m 590-0729Cable for relay contact 10 m 590-0730Level sensor, singular 590-0737

Cap Tubing Ports Cat. No.

S 55 3 ports for 2.3/3.2 mm OD 590-0766S 55 2 ports for 2.3/3.2 mm OD 1 1 leak port for 6-8 mm ID tubing 590-0767S 60/61 3 ports for 2.3/3.2 mm OD 590-0777S 60/61 2 ports for 2.3/3.2 mm OD 1 1 leak port for 6-8 mm ID tubing 590-0779S 65 3 ports for 2.3/3.2 mm OD 590-0768S 65 2 ports for 2.3/3.2 mm OD 1 1 leak port for 6-8 mm ID tubing 590-0769S 70/71 3 ports for 2.3/3.2 mm OD 590-0781S 70/71 2 ports for 2.3/3.2 mm OD 1 1 leak port for 6-8 mm ID tubing 590-0783B 83 3 ports for 2.3/3.2 mm OD 590-0770B 83 2 ports for 2.3/3.2 mm OD 1 1 leak port for 6-8 mm ID tubing 590-0771S 90 3 ports for 2.3/3.2 mm OD 590-0773S 90 2 ports for 2.3/3.2 mm OD 1 1 leak port for 6-8 mm ID tubing 590-0774

Touchless level control

Level control for conductive PE-HD


For more information on these products complete the faxback enquiry form on the backcover and send to your local VWR sales office orvisit our website www.vwr.com and click on equip.

Greater protection and reliability in weighing hazardous powdered compoundsFor researchers and laboratory technicians, the risks involved with handling toxic and high-potencycompounds are considerable. Weighing even the smallest sample quantities can expose personnelto potential hazards. Appropriate protective measures must be taken to protect the health of alllaboratory staff when they weigh hazardous compounds in powdered form.

The Sartorius SWC Safety Weighing Cabinet offers important advantages in design andconstruction over traditional laboratory hoods: The SWC models create a contained area aroundthe laboratory balance which prevents any air or finely powdered particulates from escaping intothe laboratory environment. At the same time, the constant inlet air velocity and the specialcabinet construction both keep the air current practically turbulence-free and as a result, ensureconsistent and repeatable weighing results.

Achieve minimum weight requirements according to USPThe balance and weighing cabinet are perfectly matched to each other. With the SWC SafetyWeighing Cabinet, Sartorius has succeeded in uniting two otherwise contradictory requirements:maximum personnel protection and reliable weighing results. The original Sartorius balanceperformance specifications are maintained and therefore the expected minimum sample quantitiesare unaffected.

Each of the four basic models consists of: Safety Weighing Cabinet with a separate HEPA filterunit, data-logging alarm, lighting unit, waste disposal system (on one side), airflow smoke test kitand antistatic cleaning wipes.

Sartorius SWC Safety Weighing Cabinets comply with the requirements of EN14175. The calibratedairflow alarm system with audit trail function sounds an alarm whenever the airflow velocity dropsbelow a defined level. The filter unit with an HEPA H14 filter is freestanding to effectively preventvibrations. The "bag in, bag out" filter system allows the filter to be changed quickly and withoutcontamination.

All of the balances listed below were tested for use in the Safety Weighing Cabinet (SWC) andachieved their typical repeatability when their digital filter is adjusted accordingly.

Microbalances: ME5 & ME36SSemi-microbalances: ME235S, ME235P Analytical Balances: All ME, LA, CP & ED seriesPrecision Balances: All LA, CP & ED series milligram models plus special –DS version 0,01g modelshaving an analytical style draft shield.

Personnel safety and reliable weighing results,from a single source

Applications

The filter unit with HEPA H14 filter isfreestanding, which eliminates vibrations fromthe separate cabinet and the “bag in, bagout” filter system means that the filter can bechanged quickly and without contamination.

Special bundle prices are available!

For example:

• Purchasing the safety weighing cabinet including fan filtration unit

• Get the cabinet and balance in a combo!

Contact your local VWR sales office for details of this plus other package prices.



The UncertaintyManager® is a new softwaretool for the rapid determination of themeasurement uncertainty of analytical resultsaccording to international standardsEurachem / CITAC Guide "QuantifyingUncertainty in Analytical Measurement" and"ISO Guide to the Expression of Uncertainty inMeasurement" (GUM).

The software, based on a substantial database,comprises numerous functions that facilitatedata entry, enable critical parameters to bevisualised, calculate the measurementuncertainty and the compilation of reports. Italso displays information on the contribution touncertainty of the various parameters involved(see fig.4); this helps to minimise themeasurement uncertainty of both existing andnew analytical methods.

Measurement uncertainty can be calculated intwo ways: either using the uncertaintypropagation method or using the currentlypreferred Monte Carlo method, whichadditionally gives information on the uncertaintydistribution of the analytical result (see fig. 5).

Not only can the UncertaintyManager® beused for the calculation of the uncertainty of

existing procedures; it can also beused to simulate and optimise newprocedures and is therefore avaluable tool in methoddevelopment as well.

UncertaintyManager® - the time and cost saving tool for assessing measurement

uncertainty of your analytical results

UncertaintyManager® Version 3.0The quick and reliable way to evaluate measurement uncertainty in analytical chemistry

How reliable and meaningfulare analytical results? This becomes particularlyimportant if the results obtainedare used for making decisions inthe areas of health and safety orhave legal implications. In orderto be able to evaluate analyticalresults properly, one has toknow the degree of uncertaintyinvolved with the methodsemployed. Therefore,determination of measurementuncertainty is now beingdemanded by internationalstandards authorities (e.g. ISO 17025).

Manual determination of themeasurement uncertainty of analyticalresults according to ISO is extremelycomplex and very time consuming.

Fig.4: Result: Analytical measurement result with its absolute and relative standard measurementuncertainty. The histogram below shows theindividual uncertainty contributions and theirminimisation potentials.

Fig.2: Selection of analytical technique andprogramming wizard for input of themeasurement equation

Fig.3: Illustration of the analytical procedureincluding sample preparation for thesample (left) and for the reference (right).

Fig. 5: Uncertainty distribution of the analyticalresult after uncertainty calculation using theMonte Carlo simulation method. The linesindicate the width of the error bar for the1σ, 2 σ and 3 σ case, respectively.

Fig.1: If threshold values have been exceededor whether a product meets itsspecifications, for instance, can only bedetermined if the uncertainty ofmeasurement is taken into account.

UncertaintyManager® Version 3.0 containsmodules for the following analyticaltechniques (see Fig.2) :• Volume, Mass & Concentration• HPLC and HPLC-MS• Ion Chromatography and IC-MS• GC and GC-MS• Photometry• Titration• ICP-OES*• ICP-MS• Comprehensive inorganic and organic

sample preparation

* Measurement uncertainty of AAS methods canbe calculated using the ICP-OES module.

UncertaintyManager®3.0,

could save you days of

work and effort

AustriaVWR International GmbHGraumanngasse 71150 WienTel.: 01 97 002 0Fax: 01 97 002 600E-mail: [email protected]

BelgiumVWR International bvba/sprlHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenTel.: 016 385 011Fax: 016 385 385E-mail:[email protected]

DenmarkVWR - Bie & BerntsenTransformervej 82730 HerlevTlf.: 43 86 87 88Fax: 43 86 87 90E-mail: [email protected]

FinlandVWR International OyPihatörmä 1 C 102240 EspooTel.: 09 80 45 51Fax: 09 80 45 52 00E-mail: [email protected]

FranceVWR International S.A.S.Le Périgares – Bâtiment B201, rue Carnot94126 Fontenay-sous-Bois cedexTel.: 0 825 02 30 30 (0,15 EUR TTC/min)

Fax: 0 825 02 30 35 (0,15 EUR TTC/min)

E-mail: [email protected]

GermanyVWR International GmbHHilpertstrasse 20aD - 64295 DarmstadtTel.: 0180 570 20 00*Fax: 0180 570 22 22*E-mail: [email protected]*14 Cent/Minute aus d. dt. Festnetz

IrelandVWR International Ltd Orion Business Campus Northwest Business Park Ballycoolin Dublin 15 Tel: 01 8822222 Fax: 01 8822333 Email: [email protected]

Northern IrelandVWR International Ltd A10 Harbour Court, 7 Heron Rd Sydenham Business Park Belfast BT3 9HB Tel.: 028 9058 5800 Fax: 028 9080 7812 E-mail: [email protected]

ItalyVWR International s.r.l.Via Stephenson 9420157 Milano (MI)Tel.: 02 332 03 11Fax: 800 152 999E-mail: [email protected]

The NetherlandsVWR International B.V.Postbus 81981005 AD AmsterdamTel.: 020 4808 400Fax: 020 4808 480E-mail: [email protected]

NorwayVWR International ASKakkelovnskroken 1P.B. 45, Kalbakken 0901 OsloTel.: 02290Fax: 815 00 940E-mail: [email protected]

PortugalVWR International - Materialde Laboratório, LdaEdifício NeoparkRua Tomás Ribeiro, 43- 3 D2790-221 CarnaxideTel.: 21 3600 770Fax: 21 3600 798/9E-mail: [email protected]

SpainVWR International Eurolab S.L.Ronda Can Fatjó, nº 11 Edifici Tecnopark, 3 Parc Tecnològic del Vallés08290 Cerdanyola del VallésBarcelonaTel.: 902 222 897Fax: 902 430 657E-mail: [email protected]

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SwitzerlandVWR International AGLerzenstrasse 16/18 8953 DietikonTel.: 044 745 13 13Fax: 044 745 13 10E-mail: [email protected]

UKVWR International LtdCustomer Service CentreHunter BoulevardMagna ParkLutterworthLeicestershireLE17 4XNTel.: 0800 22 33 44Fax: 01455 55 85 86E-mail: [email protected]

Want to know more?Please tick off the articles that are of interest to you and fax to your local VWR sales office (see below) or email with the title “Equip information required”

Page No. Article Tick for more information3 Dry block heaters �4 Illumination systems �5 VWR Collection microscopes �6 Julabo chillers �7 Huber controllers �8 Titroline KF trace �9 Binder incubators �10 Stuart melting point range �11 Eppendorf Biophotomer plus �12 SCAT Europe level control �13 Sartorius weighing stations �14 VWR Uncertainty Manager® �15 Chromatography �Tick here if you would like to register for our monthly email newsletter packed full of offers and news �

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