VIDAS UP for testing Listeria and ... - Merieux Nutrisciences · © Silliker Australia Pty Ltd 2017 Speaker Mark Dawson Microbiology Technical Support Manager, Australia Mérieux

© Silliker Australia Pty Ltd 2017

VIDAS UP for testing Listeria and

Salmonella - what you need to know

Mark Dawson - Microbiology Technical Support Manager

1


Speaker

Mark Dawson

Microbiology Technical Support Manager, Australia

Mérieux NutriSciences


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DISCLAIMER: Information presented on INFORM presentations and webinars are for updates and information purposes only. They do not constitute legal advice or legal opinions. Mérieux NutriSciences Australia makes no claims, promises or guarantees about the accuracy, completeness or adequacy of the information presented in this INFORM webinar.


Who is Mérieux

NutriSciences?

Click on the image to play the video.

https://www.youtube.com/watch?v=qCkfhvS4LuA


MERIEUX NUTRISCIENCES CONNECT

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A cost effective integrated program of leading scientific and

technical services


CONTENTS

Introduction

Advantages and Limitations

Listeria

Salmonella

What you need to know

Take home message

© Silliker Australia Pty Ltd 2017 7

Introduction

Vidas and Vidas Up run on the same Vidas equipment. They are both ELFA (Enzyme Linked Fluorescent Assay).

Both provide Negative and Positive results

Both are a screen for enriched samples

Negative = Not Detected, test is complete, result is reported as Not Detected

Positive = Presumptive positive (also called suspect), has to be confirmed

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VIDAS UP

Parameter VIDAS VIDAS UP

TAT Second day Next day Salmonella up to 26 hours

Listeria up to 30 hours

Media Two Stages:

Primary is low selectivity;

then Secondary is more

selective

Single stage, but different

media to Vidas

Process Traditional immunological

method labelling antigens

specific to the target

bacteria that bind with

antibodies

Recombinant

Bacteriophage Protein

Technology, so still an

immunological method,

not DNA/PCR


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VIDAS

Traditional ELISA and ELFA bind with antigens recognised by antibodies. These antigens are on the cell wall of the target bacteria, in our case our targets are Salmonella and Listeria. These antigens have the disadvantage of some level of cross reaction with non-target bacteria, such as Citrobacter and Hafnia for Salmonella.

VIDAS UP

Recombinant Bacteriophage Protein (RBP) use the receptor sites for bacteriophages (virus that invades bacteria) on the cell membrane to selectively capture bacteria. They have superior stability, specificity, and affinity to carbohydrate binding proteins such as antibodies and lectins over the traditional cell wall/membrane antigens used in ELISA and ELFA. To exploit the use of RBPs, VIDAS UP uses the phage protein screens to identify binding partners that recognize the host bacterium, i.e. the target Salmonella or Listeria.


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ADVANTAGES of VIDAS UP

Next day result for Not Detected or Presumptive requiring confirmation

Earlier release of product on “Hold and Release”

Fewer false positive screening results requiring confirmation

LIMITATIONS of VIDAS UP

Not all sample matrices have been validated and some samples may not be suitable for VIDAS UP

Restricted time window for lab to receive samples to provide next day results

No Listeria monocytogenes screen option, Listeria species only


VIDAS UP LISTERIA

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VIDAS UP Listeria (LPT) First Action Official Methods of Analysis #2013.10

Certified ISO 16140 by AFNOR (BIO 12/33-05/12) in human food products and production environment samples.


VIDAS UP SALMONELLA

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VIDAS UP

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VIDAS UP LPT (Listeria) is validated for:

Food

Category Matrices

Sample

size Dairy Cheese (Queso fresco), vanilla ice cream, milk 25 g

Dairy Cheese (Queso fresco) 125 g

RTE Meat

Deli ham, chicken nuggets, chicken liver pate,

pepperoni, beef hot dogs 25 g

RTE Meat Deli ham, Deli Turkey 125 g

Raw Meat Ground beef 125 g

RTE Meal Cooked Chill meal, sandwiches 25 g

Fruits and

Vegetables Mixed salad, Cantaloupe, 25 g

Seafood Cooked shrimp/prawn, smoked salmon 25 g

Nuts and Nut

Products Peanut butter 25 g

Misc Black pepper 25 g

Environmental Environmental surfaces

per swab or

per sponge


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VIDAS UP LPT (Listeria) is not validated and therefore cannot be used until validated for:

Any sample quantity larger than listed in the table, so no 375 g samples, largest validated sample size is 125 g

Any food category not listed

Verification will be required for:

Any sample matrix not listed in the validated food category, until it has been verified, e.g. Misc – can test black pepper, but any other spice (ginger, chilli, garlic, etc.) must be verified first

We have access to the Mérieux NutriSciences corporate network verification database so we can check this first to see if verification may be required


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VIDAS UP SPT (Salmonella) is validated for:

Food Category Matrices Sample

Size Raw Meat Ground beef 25 g

RTE Meat Roast beef 25 g

Seafood Raw cod fish 25 g

Seafood Cooked shrimp 25 g

Dairy Non-fat dry milk 25 g

Dairy Cheese

(American) 25 g

Dairy Vanilla Ice cream 25 g

Egg Powdered egg 25 g

Egg Liquid egg 25 g


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VIDAS UP SPT (Salmonella) is validated for:

Food Category Matrices Sample

Size Nuts and Nut Products Peanut butter 25 g

Spices Black pepper 25 g

Fruits and Vegetables Lettuce 25 g

Environmental Environmental

samples Swab/Sponge

Raw Meat Ground beef 375 g

Pet Food Pet food 375 g

Fruits and Vegetables Produce 375 g

Nuts and Nut Products Almonds 375 g

Poultry Rinses Poultry Rinses 30mL

Carcass swabs Swab 300cm2


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VIDAS UP SPT (Salmonella) is not validated and cannot be used until validated for:

Any sample quantity larger than validated for the sample matrix (e.g Dairy Powder larger than 25 g sample)

Any food category not listed

Some Chocolate types – not all variations have been validated or verified

Verification will be required for:

Any sample matrix not listed in the category, until it is verified, e.g. spices – can test black pepper, but any other spice (ginger, chilli, garlic, etc.) must be verified first

We have access to the Mérieux NutriSciences corporate network verification database so we can check this first to see if verification is required


VIDAS UP

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What you need to know Turn around time is next day, not 24 hours. Incubation time alone

is 18-30 hours depending on the test and sample

To ensure next day results, sample receipt time at the lab is critical, especially for Listeria

Lab needs 1-2 hours from receipt to complete sample registration and setup

Incubation time is 22-26 hours for Listeria test environmental samples and 26-30 hours raw material/product samples

Incubation time for Salmonella is 16-26 hours (18-24 hours for most samples). The time depends on both the sample matrix and the sample size.

Note that some dairy products (e.g. raw milk cheese) also require a 6-8 hour second enrichment making a total incubation time of 24-32 hours


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What you need to know (cont.) VIDAS UP analysis time is 1.5 hours

Data checking and issue/release of COA is 0.5 to 1 hour

Therefore, total time is 27-31 hours for environmental samples and 31-35 hours for raw material/product samples for Listeria

If you as a client want next day COA by 5pm, sample will have to be in sample registration preferably by 10 am, and absolutely no later than11 am. If received later, sample will have to be set up following day to comply with validated incubation times

Lab will need to be aware that VIDAS UP sample is due to be delivered so that suitable staff are rostered to process sample


VIDAS UP

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Take Home Message: All of our labs are NATA accredited for Listeria and Salmonella by

VIDAS UP

Check with us that your sample matrix can be tested by VIDAS UP without having to first verify or validate

Remember the delivery/receipt time requirements at the LAB when sending samples for testing

Remember to advise the Lab to make sure that the Lab is aware of incoming samples. This will ensure they meet setup deadline to achieve next day result reporting to you


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VIDAS UP for testing Listeria and ... - Merieux Nutrisciences · © Silliker Australia Pty Ltd 2017 Speaker Mark Dawson Microbiology Technical Support Manager, Australia Mérieux