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BACKGROUNDNecrotic enteritis from Clostridium perfringens and infection from Escherichia coli are two of the most frequently identified bacterial diseases in broiler flocks. Biotoxins from these bacteria can result in significantly depressed weight gain with associated increases in flock mortality; a heavy economic burden on producers.
Numerous studies have demonstrated the ability of Varium™ (Amlan International, Chicago, IL) to mitigate necrotic enteritis. Recently the United States Department of Agriculture’s Agricultural Research Service (USDA-ARS) analyzed the compound’s ability to neutralize the pathogenic biotoxins of both C. perfringens and E. coli.
Researchers at the USDA-ARS developed specific enzyme-linked immunosorbent assays (ELISA) for in-vivo detection of the pathogenic toxins of interest. Varium was combined with each toxin at various ratios to determine the capacity of Varium to neutralize the biotoxins. Post-incubation, each combination was centrifuged to separate free and Varium-bound toxins. The supernatant was then analyzed to quantify the presence of unbound toxins.
USDA-ARS IN-VITRO ANALYSIS Varium™ Biotoxin Neutralization
UNITED STATES DEPARTMENT OF AGRICULTUREBeltsville, Maryland, United States
PATENT PENDING
Technical Bulletin
Volume 600
DISCUSSIONBased on these results, Varium is clearly effective in biotoxin neutralization. These findings support in vivo research showing
that routine supplementation with Varium is an effective approach to decrease the effects of the bacterial disease necrotic
enteritis. In these experiments Varium neutralized biotoxins associated with necrotic enteritis and other costly diseases.
Amlan International410 N. Michigan Ave. Suite 400Chicago, Illinois 60611, U.S.A.
www.amlan.com+312-321-1887
Varium neutralizes biotoxins associated with necrotic enteritis. Varium neutralizes biotoxins associated with enteric diseases.
C. PERFRINGENS TOXINS
0
25
50
75
100
70
10090
100
α toxin NetB toxin
125µg/ml
250µg/ml
125µg/ml
250µg/ml
E. COLI TOXINS
0
25
50
75
100 90
100 100100
LTBHeat LabileEnterotoxin
ShigaShiga-like Toxin Type 2 protein/
Subunit B
125µg/ml
250µg/ml
125µg/ml
250µg/ml
©2015, Amlan International 10-2015
