2nd International Forum on Sustainable

Future in Asia

2nd NIES International Forum

Date January 26-28, 2017

Venue Udayana University, Bali, Indonesia

Organizer

National Institute for Environmental Studies (NIES - Japan)

Co-organizers

Integrated Research System for Sustainability Science, The

University of Tokyo (IR3S, UTokyo - Japan)

Udayana University (UNUD - Indonesia)

Bogor Agricultural University (IPB - Indonesia)

Asian Institute of Technology (AIT - Thailand)

2nd

International Forum on Sustainable Future in Asia 2

nd NIES International Forum, Bali, Indonesia, Jan. 26-28, 2017

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3

Background and Objectives

The world is now gearing toward the realization of sustainable societies. Over the past few

years, the international community has adopted several historic agreements such as the

Sustainable Development Goals (SDGs) by the UN General Assembly and the Paris Agreement at

UNFCCC COP21 in Paris. In this regard, it is important to drive the major steps taken by national

and regional stakeholders including governments, the private sector, citizens, and NGOs in

meeting the global commitments, especially those related to climate change, waste management,

biodiversity, environmental risks, and the well-being of society in general. Based on this

situation, the National Institute for Environmental Studies (NIES) is undertaking a broad range

of environmental research toward the realization of sustainable societies in an interdisciplinary

and comprehensive manner. In FY 2016, NIES established five issue-oriented research

programs—the Low-Carbon Research Program, Sustainable Material Cycles Research Program,

Harmonization with Nature Research Program, Research Program on Health and Environmental

Safety, and Environment-Economy-Society Integration Research Program—and will pursue

these programs in an integrated manner that transcends individual fields. NIES has also

established Fukushima Branch, where it is conducting the Environmental Emergency Research

Program.

In order to disseminate the outcomes of these research activities at NIES as well as to bridge the

gaps between science and policy, NIES initiated the Forum on Sustainable Future in Asia in

collaboration with The University of Tokyo and the Asian Institute of Technology in 2016. The

Second International Forum on Sustainable Future in Asia will be held from January 26-28,

2017, at Kampus Sudirman, Universitas Udayana, Denpasar, Indonesia. The forum will focus on

five key areas for the realization of sustainable societies in Asia: Environmental Monitoring,

Environmental Risks and Health, Biodiversity, Waste Management and Recycling, and

Synergizing Adaptation and Mitigation Actions. The participants will include specialists from

academia, business circles, and central/local governments in the Asia region.

The expected outcomes of the forum are to elucidate new ways of building sustainable societies

in Asia by stimulating discussions among the stakeholders, to catalyze policy-research

networking for the formulation of relevant science-based policies, and to determine feasible

paths toward sustainable societies in Asia by converting aspirations into actions at the local,

national, and regional levels. Another expected outcome of this forum is to create the foundation

for a consortium of relevant institutions and governments in the region that, through periodic

gatherings of researchers and policy-makers, will facilitate exchanges of knowledge gained

through research and catalyze dialogue at the policy-research interface in the spheres of

environmental management and sustainable development in Asia.

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Community Structure of Mangrove Ecosystem in Nusa Lembongan, Nusa Penida

District, Bali

Made Ayu Pratiwi

Udayana University

Variation of Podocarpus imbricatus at Pohen Hill, Tabanan, Bali

Made Pharmawati

Udayana University

Morphometric Characteristics Of Nyalian Fish In Buyan Lake And Beratan Lake,

Bali

G.R.A. Kartika

Udayana University

MSW Management at DKI Jakarta for Climate Change Mitigation

Rias Parinderati

Bogor Agricultural University

The Use of Horizontal Sub-Surface Flow Constructed Wetlands to Treat Laundry

Wastewater

Gede Herry Purnama

Udayana University

Vulnerability and Adaptation of Cocoa Farming in Bali, Indonesia

Ibnu Budiman

Su-re.co

Implementation of Management and FisheriesConservation (Case Study

Lemuru Fisheries at Bali Strait)

Nyoman Dati Pertami

Study Program of Aquatic Resources Management, Faculty of Marine and Fishery,

Udayana University

Sea Level Rise Impact on Eastern Coast of North Sumatra, Indonesia

Jonson Lumban

IPB

Catch per unit effort (CPUE) and fishing season index (FSI) of Bali sardinella

(Sardinella lemuru Bleeker 1853) in Pengambengan Port, Bali, Indonesia

Rani Ekawaty

Udayana University

Water Quality Condition and Heavy Metal Chromium (Cr) Content

in Tukad Badung River, Denpasar, Bali

Alfi Hermawati Waskita Sari

Udayana University

The Peformance of Male Local Rabbit that Given Commercial Feed

Supplemented by Cod Liver Oil

Ni Gusti Ayu Manik Ermayanti

Udayana University

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Variation of Podocarpus imbricatus at Pohen Hill, Tabanan,

Bali

Made Pharmawati Biology Department, Faculty of Mathematics and Natural Sciences, Udayana University

Jalan Kampus Bukit Jimbaran, Badung 80364, Bali, Indonesia

made_pharmawati@unud.ac.id

Abstract - Podocarpus imbricatus Blume is member of Podocarpacea. In Bali, P. imbricatus can be

found at Pohen Hill, Tabanan Regency. This species is one of the dominant species at Pohen Hill.

This study aims to determine genetic variation of P. imbricatus at Pohen Hill using RAPD (Random

Amplified Polymorphic DNA). The genetic diversity of P. imbricatus need to be studied to gain

information used for conservation of this species. Leaf samples were collected from Pohen Hill,

Candikuning, Baturiti, Tabanan. DNA was extraction using CTAB method followed by chloroform:

isoamylalcohol purification and ethanol precipitation. RAPD analysis was done using polymerase

chain reaction using four primers. PCR products were visualised using agarose gel electrophoresis and

ethidium bromide staining. Result showed that there was low genetic variation detected among

samples.

Keywords: Pohen Hill, Podocarpus imbricatus, Polymorphism, RAPD

2nd

International Forum on Sustainable Future in Asia 2

nd NIES International Forum, Bali, Indonesia, Jan. 26-28, 2017

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Contact Us

National Institute for Environmental Studies (NIES)

16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

Phone: +81 29 850 2227 E-mail: forum@nies.go.jp

Center for Remote Sensing and Ocean Sciences

(CReSOS) Udayana University

Udayana University Post Graduate Building, 3F PB Sudirman Street, Denpasar, Bali 80232, Indonesia

Phone/Fax: +62 361 256 162 E-mail: cresosunud@gmail.com

Variation of Podocarpus imbricatusat Pohen Hill, Tabanan, Bali

Made PharmawatiBiology Department, Faculty of Mathematics and Natural Sciences, Udayana University

Jalan Kampus Bukit Jimbaran, Badung 80364, Bali, Indonesia

Podocarpus imbricatus (Podocarpaceae) is anative species of Indonesia. It is distributed inSumatra, Jawa, Sulawesi, Lesser Sunda andPapua

In Bali, P. imbricatus grow at Forest Pohen Hill,Candikuning, Tabanan Regency. Figure 1 showspicture of P imbricatus at Pohen Hill. P. imbricatusis a long live pioneer species

Fig. 1: P. imbricatus at Pohen Hill, Candikuning, Tabanan, Bali

The aim of this study was to determine genetic variationof P. imbricatus at Pohen Hill using RAPD (RandomAmplified Polymorphic DNA).

Introduction

Materials and Methods

Leaf samples of P. imbricatus were collected fromPohen Hill, Candikuning, Baturiti, Tabanan Regency,Bali. As many as 16 individuals were sampled

DNA was extraction from 0.1 g leaf using CTAB methodfollowed by chloroform:isoamylalcohol purification andethanol precipitation

PCR – RAPD reaction was conducted using 20 µlreaction containing 25 ng DNA . The primers used wereoperon primers (OP) and primer from University ofBristish Columbia (UBC); OPA 4 (AATCGGGCTG).OPD 14 (CTTCCCCAAG), UBC 106 (CGTCTGCCCG),and UBC 250 (CGACAGTCCC).

Results and Discussion

DNA extraction resulted in DNA at concentration rangedfrom 200 ng/µl to 400 ng/µl. Smeared DNA was observed(Figure 2).

Fig. 2: Result of DNA electrophoresis from P. imbricatus

The number of amplified band, size range and level ofpolymorphism are shown in Table 1. Figure 3 showed PCRproduct using primer UBC 106.

Primer Number ofBand

Size Range(bp)

Percentage ofPolymorphis

mOPA4 2 422-300 50

OPD14 1 415 0UBC106 5 408-230 80UBC250 2 410-250 0

Average 32.5

Table 1: Number of band and percentage of polymorphsm of PCRproducts

Fig. 3: PCR product using UBC 106. Numbers above figure aresample number

PCR analysis of P. imbricatus showed averagepolymorphism of 32.5% (Table 1). This indicates that thediversity of P. imbricatus at Pohen Hill need to beincreased. Further study need to be conducted to analysedwhether there was inbreeding depression in population ofP. imbricatus at Pohen Hill.

Acknowledgement

The author thanks Directorate of Higher Education of Indonesia for research funding through Hibah Unggulan Perguruan Tinggi