Usulan Proposal Magang bakteri aeromonas hydrophila

8/19/2019 Usulan Proposal Magang bakteri aeromonas hydrophila

1/12

RENCANA KERJA MAGANG

METODE UJI TANTANG IKAN MAS (Cyprinus carpio) STRAIN RAJADANU

TERHADAP BAKTERI Aeromonas hydrophilla DI BALAI PENELITIAN

PEMULIAAN IKAN (BPPI) SUKAMANDI – SUBANG,JAWA BARAT

Oleh :

MUTHIA RAIDHA

B0A0101!

KEMENTERIAN RISET, TEKNOLOGI DAN PENDIDIKAN TINGGIUNI"ERSITAS JENDERAL SOEDIRMAN

#AKULTAS BIOLOGI

PROGRAM STUDI D III PENGELOLAAN SUMBERDA$A PERIKANAN

PURWOKERTO

%01!


2/12

LEMBAR PENGESAHAN

RENCANA KERJA MAGANG

METODE UJI TANTANG IKAN MAS (Cyprinus carpio) STRAIN RAJADANUTERHADAP BAKTERI Aeromonas hydrophilla DI BALAI PENELITIAN

PEMULIAAN IKAN (BPPI) SUKAMANDI – SUBANG, JAWA BARAT

Oleh :

MUTHIA RAIDHA

B0A0101!

Diaj!a" #e$a%ai Pe&'a" Pela!#aa" Ma%a"%

Pa&a P'%a S*&i D+III Bi'l'%iBi&a"% Keei"a*a" Pe"%el'laa" S$e&a-a Pei!a"a"

.a!l*a# Bi'l'%i U"i/e#i*a# Je"&eal S'e&ia"

Di#e*ji &a" &i#ah!a",

Pa&a *a"%%al0000000000000000000000000000000000

D'#e" Pe$i$i"%, Pe$i$i"% Laa"%a",

D#0 I"&aa1a", M0S, .la"&ia"*' Sih Palii', S0Si NIP0 23452678 239365 2 667 NIP0 23846529 762465 2 662

Me"%e*ahi,

Wa!il De!a" Bi&a"% A!a&ei! Ke*a P'%a S*&i.a!l*a# Bi'l'%i U"#'e&, D+III PSDP,

D0 He"&' Pa'"', M0S, D#0 I"&aa1a", M0S,

NIP0 23436977 23862 2 662 NIP0 23452678 239365 2 667

ii


3/12

KATA PENGANTAR

Pji #-! !eha&ia* Allah SWT -a"% *elah eliah!a" aha* &a"

hi&a-ah+N-a, #ehi"%%a e"li# &aa* e"-ele#ai!a" Re";a"a Keja Ma%a"%

&e"%a" j&l 'a#i, e$ei!a" a#!a" ei!ia"0 Se'%a Re";a"a Keja Ma%a"% i"i

$ea">aa* $a%i e$a;a0

P1'!e*', .e$ai 762

Pe"li#

iii


4/12

DA#TAR ISI

HALAMAN JUDUL 000000000000000000000000000000000000000000000000000000000000000000000000000000000000000i

LEMBAR PENGESAHAN 0000000000000000000000000000000000000000000000000000000000000000000000000000ii

KATA PENGANTAR 0000000000000000000000000000000000000000000000000000000000000000000000000000000000000iii

DA.TAR ISI 00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000i/

I0 PENDAHULUAN 0000000000000000000000000000000000000000000000000000000000000000000000000000000000000002

A0 La*a Bela!a"% Ma%a"% 0000000000000000000000000000000000000000000000000000000000000000000000002

B0 Ra"% Li"%! Ma%a"% 0000000000000000000000000000000000000000000000000000000000000000000000007

?0 Ga$aa" U I"#*a"#i 0000000000000000000000000000000000000000000000000000000000000000000005D0 Tja" &a" Ma">aa* 000000000000000000000000000000000000000000000000000000000000000000000000000000@

II0 PELAKSANAAN MAGANG 000000000000000000000000000000000000000000000000000000000000000000 4

A0 Be"*! Ke%ia*a" Ma%a"% 00000000000000000000000000000000000000000000000000000000000000000000004B0 P'#e& Keja Ma%a"% 00000000000000000000000000000000000000000000000000000000000000000000000004

III0 JADWAL KEGIATAN MAGANG ..................................................

9

DA.TAR PUSTAKA 0000000000000000000000000000000000000000000000000000000000000000000000000000000000000008

i/


5/12

I& PENDAHULUAN

A& L'' Bel'*'+ M''+

I"&'"e#ia ea!a" "e%aa !elaa" -a"% eili!i $a"-a! la, $ai! la $e#a a" !e;il0 Peaia" la* I"&'"e#ia eili!i la# -a"%

eli"%!i #e!i*a 75 &ai *'*al la# eaia" I"&'"e#ia0 Se;aa ,

eaia" &i$a%i e"ja&i *i%a !el''! -ai* eaia" *a1a, a-a, &a" la*0

Se*ia !el''! eaia" eili!i !e"%%la" &ala ea">aa*a""-a -a"%

&i#e#ai!a" &e"%a" !e$*ha" e"&&! I"&'"e#ia0 Salah #a* !e%ia*a"

e"&&! I"&'"e#ia &ala ea">aa*!a" eaia" *e#e$* #e;aa '*ial

a&alah $&i&a-a i!a"0

I!a" a# (Cyprinus carpio) ea!a" #alah #alah #a* je"i# i!a" ai

*a1a -a"% &i$&i&a-a!a" 'leh e*a"i i!a", lai &ai e$e"iha",

e"&e&ea" hi"%%a e$e#aa" &i !'la e!aa"%a" a*a" ai &ea#0 Di

!ala"%a" e*a"i i!a", i!a" a# *elah laa &i!e"al &a" &i#!ai (&i!'"##i)

#ehi"%%a ea#aa""-a *i&a!lah #li* (Sa"*'#', 2385)0 I!a" a# $ea#al &ai

?i"a &a" *e#e$a !e A#ia, E'a, #e$a%ia" Aei!a U*aa, &a" A#*alia0 I!a"

a# #e$a%ai i!a" !'"##i ea!a" #alah #a* !''&i*a# #e!*' ei!a"a"

ai *a1a -a"% *e# $e!e$a"%0 I!a" a# $a"-a! &ii"a*i !'"#e" !ae"a

a#a &a"%i"%"-a -a"% e"a! &a" %ih #e*a eili!i !a"&"%a" '*ei" -a"%

;! *i"%%i0 Pei"*aa" !'"#e" i!a" a# &ai *ah" !e *ah" ;e"&e"%

e"i"%!a* *e*aa &i !'*a+!'*a $e#a, #ee*i Ja!a*a, Sa$a-a, &a"

Ba"&"% (Khaia" et al., 7667)0

I!a" a# -a"% &i$&i&a-a!a" &i Balai Pe"eli*ia" Peliaa" I!a"

S!aa"&i, S$a"%, Ja1a Baa* *e&ii a*a# lia strain -ai* i!a" a#

Raja&a", i!a" a# Si"-'"-a, i!a" a# Majala-a, i!a" a# S*i#"a, &a" i!a"

a# Wil&a"0 I"&! i!a" a# Raja&a" $ea#al &ai &aeah K"i"%a", i!a"

a# Si"-'"-a $ea#al &ai &aeah Pa"&e%la"%, i!a" a# Majala-a $ea#al &ai

Ba"&"%, i!a" a# S*i#"a $ea#al &ai K"i"%a", &a" i!a" a# Wil&a"

$ea#al &ai ?ia"j0 I!a" a# strain Raja&a" ea!a" i!a" -a"% #aa* i"i

2


6/12

e"ja&i '$-e! e"eli*ia" &i BPPI S!aa"&i0

Uji *a"*a"% a&a i!a" a# Raja&a" *eha&a $a!*ei A. hydrophila -a"%

&ila!!a" &i BPPI S!aa"&i a&alah &e"%a" ;aa e"%i"je!#ia" ;aia"

$a!*ei A. hydrophila. Pela!a" -a"% #aa, #e$a%ai e$a"&i"%, &i%"a!a"

a&a i!a" a# Ma"*a &a" i!a" a# &ai UPR (U"i* Pe$e"iha" Ra!-a*)

-a"% !e&a"-a ea!a" strain Majala-a0 Pe"%jia" &ila!!a" &e"%a" 5

!ali la"%a" #elaa 28 ja &ala e"*a"% i"*e/al e"%aa*a" e ja0

Ba!*ei A. hydrophila ea!a" #alah #a* $a!*ei e"-e$a$ e"-a!i* -a"%

$e$aha-a a&a $&i&a-a i!a" ai *a1a0 Ba!*ei *e#e$* $a"-a! e"-ea"%

i!a" a# -a"% ea!a" #alah #a* !''&i*a# "%%la" ai *a1a &a" &aa*

e"%i">e!#i i!a" a# a&a #ea &a" !a" -a"% &aa* e"-e$a$!a"!ea*ia" hi"%%a e";aai 860 Hal *e#e$* &aa* e"%a!i$a*!a" !e%ia"

-a"% #a"%a* $e#a &ala #aha $&i&a-a i!a" ai *a1a (Sa"'e#i, 7668)0

A. hydrophila &i!e"al #e$a%ai e"-a!i* Motile Aeromonas

Septicemia a*a e"-a!i* $e;a! eah0 C'%a"a"*h et al., (7663) e"-a*a!a"

$ah1a A. hydrophila ea!a" i!''%a"i#e a!a*i! -a"% $ea&a

&ieaia" la* a" eaia" *a1a0 Dala !'"&i#i i!a" #*e#, $a!*ei

*e#e$* e"ja&i a*'%e" &a" $e#i>a* a*'%e" ''*"i#*i! a&a e"-a!i*

hemoragi septicemia (e"-a!i* $e;a! eah) a&a i!a"0 I!a" -a"% *e#ea"%

$a!*ei *e#e$* a!a" e"%alai e"&aaha" a&a $a%ia" *$h, *e*aa

a&a $a%ia" &a&a, e* &a" a"%!al #ii0 Ba!*ei A. hydrophila &aa*

e"i$l!a" !e%ia" -a"% #a"%a* $e#a $ah!a" a&a !'la -a"% &ia1a*

&e"%a" $ai!, #ehi"%%a &aa* e"i$l!a" !ea*ia" i!a" #e;aa a##al0

B& R-'+ L.+*-/ M''+

Balai Pe"eli*ia" &a" Peliaa" I!a" (BPPI) S!aa"&i, S$a"%, Ja1aBaa* ea!a" #alah #a* $alai e"eli*ia" -a"% ela!!a" !e%ia*a"

e"eli*ia" &ala $i&a"% %e"e"*i! 'le!le, e"%jia" /i# KH, !ali*a#

ai, "*i#i &a" a!a" alai -a"% &iaah!a" "*! e"%e$a"%a" i!a" Nila,

Ma#, Lele, Pa*i", Gae, &a" U&a"% Galah0 Ra"% li"%! !e%ia*a" a%a"%

7


7/12

i"i a&alah ela!!a" #e;aa a!*i> ji *a"*a"% a&a i!a" a# Raja&a" &e"%a"

i!a" a# e$a"&i"%"-a *eha&a $a!*ei A. hydrophila0

C& G'''+ U- Pe-2'h''+

Balai Pe"eli*ia" Peliaa" I!a" (BPPI) S!aa"&i &i&ii!a" a&a

*a"%%al 7 J"i 23790 Selaa e!e$a"%a""-a *elah e"%alai $e$eaa

e$aha" "aa0 Balai Pe"eli*ia" Peliaa" I!a" S!aa"&i, S$a"%, Ja1a

Baa* *ele*a! &i Jala" Ra-a 7 S!aa"&i, De#a Ra";al-a, Ke;aa*a"

Pa*'!$e#i, Ka$a*e" S$a"%, P'/i"#i Ja1a Baa*0 Balai Pe"eli*ia"

Peliaa" I!a" (BPPI) S!aa"&i e"-ai La# aeal #e!i*a 6 ha *e&ii

&ai 55,@ ha aeal "*! e!'laa" *ea#! e#e/'i &a" #ala" ai a#!,

2,89 ha "*! e!a"*'a" &a" 23,95 ha &i%"a!a" "*! ha*;he- &a"

eaha" !a-a1a" #e*a #aa"a e"&!"% lai""-a0 Be&a#a!a" KEPMEN

Kela*a" &a" Pei!a"a" N'0 KEP0 69MEN7665, Balai Pe"eli*ia" Peliaa"

I!a" (BPPI) S!aa"&i ea!a" le$a%a i#e* &i $i&a"% eliaa" &a"

*e!"'l'%i $&i&a-a ei!a"a" ai *a1a -a"% $ea&a &i $a1ah Ba&a"

Pe"eli*ia" &a" Pe"%e$a"%a" Kela*a" &a" Pei!a"a" (BALITBANG KP)

&a" $e*a"%%"%ja1a$ la"%#"% !ea&a P#a* Ri#e* Pei!a"a" B&i&a-a

(PRPB), Balai Pe"eli*ia" Peliaa" I!a" $e*ja" "*! e$e"*! #a*

le$a%a -a"% #e;aa '*ial a ela!#a"a!a" i#e* eliaa" &a"

*e!"'l'%i $&i&a-a ei!a"a" a&a #ea e!'#i#*e ai *a1a #ehi"%%a

a ea!i* *e!"'l'%i #*a*e%i# &a" ali!a*i> &ala $i&a"% eliaa" &a"

*e!"'l'%i $&i&a-a ei!a"a" &ala a"%!a e"i"%!a*a" &a" !e$ela"j*a"

'&!#i $&i&a-a i!a" #e;aa "a#i'"al0

Saa"a &a" Pa#aa"a -a"% *e&aa* &i Balai Pe"eli*ia" &a" Peliaa"

I!a" (BPPI) S!aa"&i, S$a"%+Ja1a Baa* a&alah !a"*' a&i"i#*a#i, e#*a!aa", a"% a&i*'i (!aa#i*a# 566 'a"%), e##, a#ji&, %e&"%

'laha%a (GOR), &a" laa"%a" a!i* -a"% ;! la#0 U"*! !e%ia*a"

e"eli*ia" &i BPPI &i&!"% 'leh la$'a*'i %e"e"*i! 'le!le -a"%

&ile"%!ai a"*aa lai" Real *ie P?R, DNA .a%e" a"al-e, Wh'le %e"'

5


8/12

SeFe";e, La$'a*'i BPPI &ala '#e# a%e&i*a#i &ala a"% li"%!

e"%jia" /i# KH &a" !ali*a# ai *e#e&ia "*! e""ja"% ela!#a"aa"

e"eli*ia" a"*aa lai": !'la *a"ah &a" !'la *e$'!, !'la reservoir ( ha),

la$'a*'i !iia &a" la$'a*'i %e"e*i!, la$'a*'i "ii#i &a" a!a"

alai, &a" la$'a*'i !ali*a# ai ha*;he- (Nila, Ma#, Lele, Pa*i", Gae,

&a" U&a"% Galah)0

D& T-3-'+ 4'+ M'+5'' M''+

Tja" &ila!#a"a!a""-a !e%ia*a" a%a"% i"i a&alah:

20 Maha#i#1a e"&aa*!a" !e*eaila" e"eaa" e*'&e ji *a"*a"% a&a

i!a" a# strain Raja&a" *eha&a $a!*ei A. hydrophila0

70 Maha#i#1a &aa* e"%e*ahi ha#il ji *a"*a"% i!a" a# strain Raja&a"

*eha&a $a!*ei A. hydrophila0

Ma">aa* Pa!*i! Ma%a"% &i Balai Pe"eli*ia" Peliaa" I!a" (BPPI)

S!aa"&i, S$a"%, Ja1a Baa* a&alah:

20 Be*a$ah"-a e"%e*aha", 1a1a#a" &a" !e*eaila" *e"*a"% e*'&e ji

*a"*a"% a&a i!a" a# *eha&a #a* $a!*ei0

70 Mee'leh e"%alaa" $e!eja &i Balai Pe"eli*ia" Peliaa" I!a"

(BPPI) S!aa"&i, S$a"%, Ja1a Baa*0

@


9/12

BAB II

PERENCANAAN MAGANG

A& Be+-* Ke.''+ M''+

Be"*! !e%ia*a" -a"% a!a" &i%"a!a" #elaa a%a"% &i Balai

Pe"eli*ia" &a" Peliaa" I!a" (BPPI) S!aa"&i, S$a"%, Ja1a Baa* a&alah

&e"%a" e"%%"a!a" e*'&e '$#e/a#ie"eli*ia" &a" a#*i#ia#i a!*i>0

Pa*i#ia#i a!*i> -a"% &ila!!a" a&alah !e%ia*a" e"%i!*i #e;aa la"%#"%

#ea !e%ia*a" -a"% $eh$"%a" &e"%a" ji *a"*a"% a&a i!a" a# strain

Raja&a" *eha&a $a!*ei A. hydrophila -a"% &ila!!a" &i Balai Pe"eli*ia"

Peliaa" I!a" (BPPI) S!aa"&i, #e&a"%!a" '$#e/a#ie"eli*ia" &ala

$e"*! e"%aa*a" la"%#"% &a" &'!e"*a#i #elaa !e%ia*a" -a"%

$eh$"%a" &e"%a" ji *a"*a"% i!a" a# strain Raja&a"0 Pa!*i! Ma%a"%

i"i a!a" &ila!#a"a!a" &i Balai Pe"eli*ia" Peliaa" I!a" (BPPI) S!aa"&i,

S$a"%, Ja1a Baa*0 Pa!*i! Ma%a"% a!a" &ila!#a"a!a" #elaa 28 hai !eja

a&a *a"%%al 5 #0&0 7 A%#*# 76240

B& P62e4- Ke3' M''+

Ke%ia*a" ji *a"*a"% i!a" a# #*ai" Raja&a" *eha&a $a!*ei A.

hydrophila i"i a!a" &ila!!a" &i &ala a"%a" (indoor ) -a"% *e&aa* &i

hatcher - i!a" a#0 U"-a !e%ia*a" ji *a"*a"% *eha&a $a!*ei A.

hydrophila i"i &ila!!a" &e"%a" $e$eaa *aha, -ai* e#iaa" #aa"a &a"

a#aa" e"%jia", ela!#a"aa" e"%jia", &a" e"%aa*a" #e*elah

e"%jia"0

20 Pe#iaa" Saa"a &a" Pa#aa" Pe"%jia"

Pe#iaa" #aa"a &a" a#aa" e"%jia" ea!a" !e%ia*a" -a"%

&ila!!a" "*! e"-ia!a" *ea* e"%jia" i!a" a#0 Pe#iaa" #aa"a

&a" a#aa" e"%jia" &ia"*aa"-a &ila!!a" e$e#iha" 28 a!ai

$e!a" @6 6 ; -a"% *ele*a! &i &ala hatchery i!a" a#, e$e#iha"

aea*', #e*a e#iaa" ;aia" $a!*ei A. hydrophila0 Pe$e#iha" a!ai

4


10/12

&a" aea*' &ila!!a" e"%%"a!a" #a$" *ele$ih &ahl0 Se*elah &ila!!a"

e$e#iha", a!ai &a" aea*' &ila!!a" e$e#iha" e"%%"a!a"

la*a" !l'i"0 La*a" !l'i" $e>"%#i e"#*eil!a" e&ia e"%jia" &ai

a*h'%e"0 A!ai+a!ai -a"% #&ah &i$e#ih!a" a#i"%+a#i"% &ii#i

ai #e$a"-a! 76 L -a"% !e&ia" &ila!!a" e$eia" $$! Thi'#l>a*0

Pe$eia" $$! Thi'#l>a* i"i %"a "*! e"#*a$il!a" #h, H, &a"

#ali"i*a# &ala a!ai -a"% #e$el"-a &ila!!a" e$e#iha"

e"%%"a!a" la*a" Kl'i", !e&ia" i!a" &i*e$a a&a a#i"%+a#i"%

a!ai0

70 Pela!#a"aa" e"%jia"

Ba!*ei -a"% *elah &i"i!a" &i%"a!a" a&a i">e!#i $a*a" "*! ea#*i!a" a*'%e"i#i*a# $a!*ei0 A!ai ha# &i*ea*!a" a&a &aeah

-a"% &ii#'la#i #ee*i &i &ala $a! #ee" &i la$'a*'i $a#ah0 Uji

a*'%i"e#i# a*a -a"% $ia#a &i!e"al &e"%a" ji *a"*a"% eili!i $e$eaa

&ala e"%jia""-a -ai* &e"%a" ee"&aa" &a" e"-"*i!a"0 Me*'&e

#"*i! le$ih &ah &i$a"&i"%!a" &e"%a" ee"&aa" !ae"a $e$eaa

>a!*' *aa &ala ji a*'%i"e#i*a# ha"-a *e!'"#e"*a#i $a!*ei0

Se$ali!"-a, e*'&e ee"&aa" eili!i >a!*' *a$aha" lai", -ai* 1a!*

ee"&aa" -a"% le$ih i*, la%i le$ih alai &i$a"&i"%!a" &e"%a" e*'&e

#"*i! &a" &aa* &i%"a!a" "*! i">e!#i $a!*ei e!#*e"al #ee*i

Flavobacterium0

50 Pe"%aa*a" #e*elah e"%jia" &ila!!a" #elaa 28 ja &i hatchery0


11/12

BAB III

JADWAL KEGIATAN MAGANG

S$a"%, 5 A%#*# #& 7 A%#*# 762@

N'0 Ke%ia*a" Mi"%% !e:2 7 5 @ 4

20 Pe#iaa" ala*

70 Pela!#a"aa" Ma%a"%

50 Pe"li#a" &a>* La'a"Ma%a"%

@0 Ujia" Ma%a"%

9


12/12

DA#TAR PUSTAKA

Khaia", S&e"&a, D0 Da"0 G"a&i, B0 76670 Budidaya Ian Mas Secara Intensi! 0

A%'e&ia0 Ja!a*a : 820

Sa"'e#i0 76680 "elacaan #en Aerolysin dari Aeromonas hydrophila pada Ian Mas yang diberi "aan $stra Ba%ang "utih0 J"al e*ei"e 25 (2) : @5+460

Sa"*'#'0 23850 &a'ian "enyait Motile Aeromonas Septicemia (MAS) "ada Ian lele

dumbo (Clasias sp.)* "atologi* "engobatan dan "encegahannya dengan

Fito!armaa0 Pa;#a Saja"a I"#*i** Pe*a"ia" B'%'0 B'%'0

C'%a"a"*h, N0, Bha!-aaj0 R0, ?ha"*h0 A0, A"$ala%a"0,T, Nila0, M0 76630

+etection o! ,irulence #ene in Aeromonas hydrophila Isolates !rom Fish

Samples -sing "CR Thecniue0 Gl'$al J'"al '> Bi'*e;h"'l'%- a"&Bi';hei#*- @ (2) : 42+450

8

Documents

Usulan Proposal Magang bakteri aeromonas hydrophila