Tvary bakteriln­ch bun›k Morfologie koloni­ Barven­ bun›k ... Micrococcus luteus Staphylococcus

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Tvary bakterilnch bunk

Morfologie koloni

Barven bunk

Interpretace Gramova barven

PGPG

Tvary bakterilnch bunkoptimln hydrodynamick vlastnosti

vyuit pstupnch ivin

Borrelia

pomr povrch/ objempomr povrch/ objemnejmen nejmen kulat bukykulat bukydlouh tenk buky nejvydlouh tenk buky nejvy

Chlamydia 0,3 x 0,3 m

Bdellovibrio 0,8 x 0,3

Rickettsia 1 x 0,3

S. aureus 0,8-1 x 0,8-1

E. coli 2-3 x 0,4-0,6

B. subtilis 1,8-4,8 x 0,9-1,1

Streptomyces vlkno x 0,7-1,6

Chromatium 25 x 10

Spirochety 500

Velikost bakteri a) nejmen

Mycoplasma:

0,2-0,8 m

- bez bunn stny

(= amorfn a nebarviteln na b.s.)

Rickettsie

0,3-0,8 m G- koky a tyinky

Barv se erven dle Giemsy,

podlouhl b. polrn.

- b.s. podobn bakteriln

Nejmen rody: rickettsie a mykoplasmata, Nanobacterium

Schopny samostatnho rstu!= nejmen voln ijc bakterie

Nerostou mimo hostitelskou buku!

Rickettsia rickettsii v

bukch endotelu cvRickettsia rickettsii

Mycoplasma pneumoniae

Nebezpe: prochz

protibakterilnmi

filtry!

Velikost bakteri b) nejvt

Epulopiscium spp. symbionti

moskch ryb Acanthuridae

Bakterie monomorfn

existence jedn morfologick formy nezvisle na podmnkch rstu

Bakterie pleomorfn (mnohotvar, pleiomorfn)

existence odlinch morfologickch forem u tho druhu i kmene (vlivem rznch podmnek pro rst, asto star kultury)

pklady:Corynebacterium diphteriae, Mycoplasma pneumoniae,Rickettsia prowazeki, Rickettsia rickettsia

1. mykobakterie 2. corynebacterium plectridium (ztlutn terminln) i

clostridium (ztlutn centrln), corynebacterium mikroskopie: jednotliv, dvojice nebo shluk.

3. aktinomycety mikroskopie: jednotliv, dvojice nebo shluky. U streptomycet nap. m del kutivace tm vt pleomorfismus.

KokyGeneticky kdovno dlen v rovinch a odlouen

samostatn buky.Spojen bunnou stnou Van Der Waalsovy sly

oplotl zapiatl

Neisseria meningitidis - Gram-negative

meningitis, Waterhouse-Friderichson syndrome

Streptococcus pneumoniae - Gram-positive Encapsulated.Causes (lower respiratory infection) pneumonia

(upper respiratory infections) bronchitis, laryngitis, sinusitis, and otitis media.

v zvislosti na rovin dlen :

diplokoky - jedno dlen v jedn rovini shluky (dle nboje buky a ivin), G+ i G-

streptokoky - dlen v jedn rovin (kad b.)Jednotliv, dvojice nebo etzky(Streptococcus, Lactococcus)

tetrdy dlen ve dvou rovinch, mlo ast, vtinou perstaj v balky

(Micrococcus luteus)

pakety, sarcinydlen ve 3 na sebe kolmch rovinch

po dlen zstvaj ve skupinch po 8

stafylokokynepravideln dlen

shluky, hroznkyShluk druhov charakteristick v prosted bohatm ivinami - vce shluk

Neisseria gonorrhoeae, Gram-negative

Micrococcus luteus

Staphylococcus

aureus

Streptococcus

Sarcina

Charakter shluk zvis na zpsobu dlen

bakterilnch bunk

piny tvorby jednotlivch typ shluk u

jednotlivch skupin bakteri nejsou jasn

myxobakterie produkce extracelulrnch

enzym a lyze nerozpustnch makromolekul

studium vztah mezi strukturou a funkc

jin distribuce ivin dovnit shluku bunk

Tyinky, tyky

rovn (vtina bakteri,E.Coli, r.Salmonella)

krtk (kokobacily)

dlouh - vlkna (Erysipelothrix,Actinomyces)

thl ( Mycobacterium tuberculosisi, Clostridium tetani)

robustn (r. Lactobacillus, Clostridium perfringens)

roztpen (r. Bifidobacterium)

vtvc se (rr. Nocardia, Actinomyces)

zakiven (vibria-Vibrio, Campylobacter) Vibria rzn prohnut na jednu stranu, divok kmeny vce ne sbrkov.Mikroskopie: vdy jednotliv, dvojice jen na konci bunnho cyklu.

s rovnmi a konkvnmi konci (Bacillus anthracis)

vetenovit (r. Fusobacterium)

kyjovit (r.Corynebacterium)

pleomorfn

Vibrio

Bifidobacterium

Mycobacterium

paratuberculosis

E. coli

Salmonella typhi

Actinomyces virosus

Kokobacilykokobacily a kokotyky dvojice nebo shluky, nikdy etzky

Bordetella pertusis, Kingella, Acinetobacter

Bordetella holmesii Acinetobacter spp.

Tyinky

Dl se typicky jen podl sv krtk osy a zstvaj vtinou oddlen;

Diplobacily:

tyky ve dvojicch s kratmi konci u sebe (nap. rod Moraxella)

Streptobacily:

Tyky, kter zstvaj v etzku po dlen (nap. Streptobacillus moniliformis,Erysipelothrix rhusiopatiae,

Bacillus, Lactobacillus, )

Moraxella

Dal tvary tyinek

Prostky tvocprostka - bunn vbek Cytoplazma +CM + BS(Filomicrobium,Hyphomicrobium)

Spirlovitnepravideln ( rody Spirilum, Helicobacter) hrub ( r.Borrelia)jemn (r.Treponema)jemn se zahnutmi

konci(r.Leptospira)

Mycelium tvoc aktinomycety, streptomycety

Helicobacter

TreponemaLeptospira

Pupeny,puenPupeny na krtk stran.Pu vtinou tyky. Pupen vdy opout mateskou buku. (Ancalomicrobium,Blastobacter,Hyphomonas)

Asticacaulis

Streptomyces

G+ vlkna

Prosthecomicrobium

Simonsiella

Cylindrospermum

Rhodomicrobium

Thiopedia rosea

Streptomyces

Spirilly urit a konstantn poet zvit( max 5-7.) a vdy stejn stoupn

Pohyb a udrovn potu zvit vdy dle osovho vlkna, zvity jen v 1 rovin, relativn tenk buky.

Spirochety vce zvit ve 2 i 3 rovinch, tlust buky.

Biky v hornm periplazmatickm prostoru, axiln biky vidme a na ezu

(jeden a nkolik destek). Undulujc membrna blkovina + sacharid v 1 rovin, vlun u vodnch.

U spiril i spirochet biky vdy na koncch, jednotliv i ve svazku.Pokud spojen bunk, tak hkem, ne plochou. Pro pozorovn se vyuv mikroskopie v zstinu. Mikroskopie: buky vdy jednotliv.

www.medmicro.info

Micrococcus

Micrococcus luteus je srov

lut (ne zlat jako

Staphylococcus aureus)

G+ sfrick buky, v prepartu jednotliv, po dvou

nebo ve tveicch i nepravidelnch shlucch

KocuriaG+ koky, po dvou, 4 nebo shluky

Bacillus cereusRovn G+ tyky rzn

dlky, ve dvojicch i

etzcch i jednotliv

Escherichia coliGramnegativn rovn tyky

Jednotliv nebo ve dvojicch

PseudomonasGramnegativn rovn nebo mrn zakiven tyky

Bakterie rodu Staphylococcusemu.arsusda.gov/typesof/pages/staph.htm

Z 10 000x

Krevn agar s 10% NaCl

Bl nazltl velk

kolonie

Mikroskop:

typick

hroznky, shluky

hemolza

Rod Bacillus rzn morfologie rznch druh

Bacillus cereus

Centrln ovln spora

Mikroskopie ve vzorku

mikroskopick prepart sputa, Gramovo b.

Grampozitivn koky, ve shlucch.

Leukocyty (hl. polymorfonuklery), svd pro

bakteriln znt

Foto: O. Z.

Vjimky pi pozorovn morfologie buky barven Gramovm barvenm

- !!Bakterie bez bunn stny (nebarviteln Gramem)

- !!Bakterie gramlabiln

- !!Pozor na acidorezistentn buky

Pozn: pro pesn tvar i men buky lpe ut negativn barven pozad

Bakterie bez bunn stny

Sekundrn ztrta b.s.

Mutac tzv. L-formy bakteri (G+ i G-)

Primrn sfrick tvar, ale i jin

ij v osmoticky stabilnm prosted (p. parazit

Mycoplasma)

Vt. specifick stavba membrny

Mal genom nepotebuj enzymy biosyntz

Borrelia burgdorferi (fig 1, 2)

Borrelia recurrentis (fig 1)

Bartonella henselae (fig 1, 2)

Chlamydia trachomatis (fig 1, images of elementary bodies, images of reticulate bodies)

Chlamydophila pneumoniae (images of elementary bodies, images of reticulate bodies)

Chlamydophila psittaci (images of elementary bodies, images of reticulate bodies)

Coxiella burnetii (fig 1, 2)

Ehrlichia chaffeensis (fig 1, 2)

Anaplasma phagocytophilum (formerly; Ehrlichia phagocytophilum or E. equi; Fig. 1)

Legionella sp. (fig 2)

Leptospira sp.(fig 1, 2)

Mycobacterium bovis (fig 1)

Mycobacterium tuberculosis (fig 1, 2 thanks to Anders Olav Lande, 3)

Mycobacterium avium, Mycobacterium intracellulare (fig 1 thanks to Anders Olav Lande)

Mycobacterium kansasii (fig 1)

Mycobacterium leprae (fig 1, for a close up thanks to Anders Olav Lande)

Mycobacterium marinum (fig 1)

Rickettsia rickettsii (Fig. 1,: scroll down to bottom of the page. 2)

Orientia tsutsugamushi (formerly; Rickettsia tsutsugamushi; Fig. 1)

Treponema pallidum(fig 1, 2, 3)

Bakterie nebarviteln Gramem

http://www.wadsworth.org/databank/borreli.htmhttp://www.cdc.gov/ncidod/dvbid/images/bburg.jpghttp://www.portfolio.mvm.ed.ac.uk/studentwebs/session4/60/Borelliaslide.jpghttp://www.cdc.gov/ncidod/eid/vol8no2/01-0141-G2.htmhttp://www.yamagiku.co.jp/pathology/case/case212.htmhttp://www.roche.com/pages/facets/1/chlamydia.jpghttp://www.chlamydiae.com/docs/biology/biol_EB.asphttp://www.chlamydiae.com/docs/biology/biol_RB.asphttp://www.chlamydiae.com/docs/biology/biol_EB.asphttp://www.chlamydiae.com/docs/biology/biol_RB.asphttp://www.chlamydiae.com/docs/biology/biol_EB.asphttp://www.chlamydiae.com/docs/biology/biol_RB.asphttp://www.theguardians.com/Microbiology/Coxiella_burnetii.jpghttp://www.safetyline.wa.gov.au/institute/level2/course20/lecture66/images/l66_06.jpghttp://www.cvm.okstate.edu/instruction/kocan/disk3/images/img0019.jpghttp://www.cvm.okstate.edu/instruction