Turbo-charged biofuel output: Moving beyond the easy sugars to access the

entire plant biomass

The only way to produce biofuel economically is to convert the entire biomass—not just the easy sugars—

of the targeted feedstock to biofuels

The Key to Success is Having the Right Microbe for the Job

Oleaginous microbes for the production of oil as a

feedstock for drop-in fuels

Advantages:• No molecular biology necessary

• Simultaneous growth optimization

• Rapid and robust selection

Experimental Evolution with BioTork Technology

Growth rate

Lipidproduction

Targettemperatures

Toxiccompounds

Carbonsources

Real world biomass

Successfully Evolved Traits

Maximizing energy yield from sweet sorghum

Why sweet sorghum?

• Can be planted in ⅔ of the US

• Tolerates poor soil, salt, dry climates

• 50% less water, nitrogen than corn

• Annual crop, two harvests per year

• Fast growing, short time to harvest

• Large quantities of both “easy sugars” and biomass

Juice•“Easy Sugars”: sucrose, glucose, fructose•Protein, other carbs

Bagasse Solubles•“Easy Sugars”: residual monosaccharides, starch•Protein, other carbs

Bagasse Insolubles•“Hard sugars”: cellulose, hemicellulose•Lignin

Carbon Sources in Sweet Sorghum

Evolved Oleaginous Microbes on Each Component

Juice

Bagasse Solubles/Insolubles

Corn Ethanol

Sweet Sorghum Ethanol

Sweet SorghumGreen Diesel(juice only)

Sweet SorghumGreen Diesel(w/bagasse)

Biofuel Yieldgal/acre 330-424 270-750 193-495 457-841

Energy Yield1000 MJ/acre 29-38 24-67 27-68 64-118

IncrementalValue$/acre

0 (84)-456 117-891 909-1929

Yield and Revenue Potential

• Highest yield for same input feedstock

• Simple fermentation technology

• Reduced waste

• Distributed biorefineries using local feedstock

• Rural development

With BioTork Technology: The right microbe for the job

Opening the door to other agricultural by-products:

Future Developments With BioTork Technology:

• Agricultural residues (stover, straw)• Biodiesel-derived waste glycerol• Woody biomass• Seed cake• Stillage• Fruit culls

Thank You…..