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MACS® Products for tissue regeneration research 3
MACS Technology—the complete solution 4
MACS Cell Separation Strategies 5
Human stem cells—sample preparation, cell separation and analysis 6
Embryonic stem cells and induced pluripotent stem cells—separation and analysis 7
Mesenchymal stromal cells—separation and analysis 8
Endothelial progenitor cells—separation and analysis 9
Tissue regeneration—examples 10
References 12
MACS Products for sample preparation, cell separation, and cell analysis 13
MACS Products for cell culture 14
Molecular biology products and services 15
Cover image: SPL/Agentur Focus
Table of contents
MACS® Products for tissue regeneration research
MACS® Products provide the basis for reliable methods to investigate the full potential of stem cells in tissue regeneration research.Stem and progenitor cells have the potential to revolutionize
tissue regeneration and engineering in the future. The
understanding of stem cell biology is of paramount importance
for the development of stem cell–based therapies. The
multipotent—and even pluripotent—differentiation capacity
of certain stem and progenitor cell populations makes them a
potential key to successful regeneration of many tissue types.
Over the coming decade, research into this field may take center
stage as the new frontier in the treatment of many disabling
diseases and injuries.
Reproducible data, standardized protocols, and defined
cell sources are key to unravel the full potential of stem and
progenitor cells. However, most stem cell sources consist of
heterogenous cell populations with varying differentiation
and regeneration potential. The availability of defined cell
populations with specific differentiation capacities will
therefore be crucial for the targeted regeneration of tissues.
In turn, this will facilitate a controlled and optimized growth
and differentiation of stem cells into their target tissue types,
especially during ex vivo tissue development. The separation of
defined pre-committed stem and progenitor cell populations
derived from stem cell populations with pluripotent or
multipotent differentiation potential would be beneficial for
future tissue regeneration applications.
Miltenyi Biotec offers numerous innovative products for the
isolation of stem and progenitor cells as well as for sample
preparation, cell analysis, cell culture, and molecular analysis.
MACS® Technology
3
MACS Products provide the tools that open up new
perspectives for tissue regeneration research:
• Reliable isolation of defined stem cell populations
with excellent purity for reproducible results
• Stem cells and progenitors thereof can be isolated
within an hour rather than several days,
saving valuable time for you to focus on your research.
• Isolated cells can immediately be used for cell culture
and animal experiments; cells remain viable and fully
functional.
• Miltenyi Biotec offers integrated solutions for a wide
variety of research needs—from sample preparation
to molecular analysis.
MACS® Technology —the complete solution
MACS® Technology
4
Since its introduction in 1989, MACS® Technology has become the gold standard for cell separation.
Nowadays, Miltenyi Biotec stands for more than cell separation, offering more than 1000 innovative
research products for biomedical research and life sciences. The MACS Research Product portfolio
includes instruments and reagents for sample preparation, cell separation, cell analysis, cell culture,
and molecular biology. Miltenyi Biotec also provides tools for clinical-scale cell separation based on
MACS Technology. Miltenyi Biotec has a strong commitment to continual product development with
regards to current and future basic and clinical research.
MACS Sample Preparation
The quality of an experiment strictly depends on the quality of the sample preparation. Miltenyi Biotec offers innovative instruments and reagents for fast and gentle preparation of samples from solid tissues as well as cultured cells.
MACS Cell Separation
A large panel of MACS MicroBeads and MicroBead Kits is available for the isolation of virtually any cell type. The cells can be separated manually or in an automated fashion. The new autoMACS™ Pro Separator has been designed for automated walk-away cell sorting of multiple samples.
MACS Cell Analysis
Miltenyi Biotec provides a large panel of monoclonal antibodies and kits for fluorescence microscopy and flow cytometry. The innovative MACSQuant™ Analyzer is an extremely compact, easy-to-use, multicolor benchtop cell analyzer. The instrument is fully automated and enables absolute cell count.
MACS Cell Culture
The product portfolio for cell culture includes media as well as recombinant cytokines and growth factors.
MACSmolecular
Miltenyi Biotec provides products for analytical protein isolation and detection, mRNA purification and amplification, cDNA synthesis and labeling, microRNA analysis, as well as microarray technologies and instrumentation. The portfolio includes genomics services, such as gene expression and microRNA analysis microarrays, array-CGH, and bioinformatics.
Magnetic labeling
Cells of interest are labeled with MACS® MicroBeads in a short incubation step.
Magnetic separation
Labeled and unlabeled cells are separated on a MACS Column placed in the magnetic field of a MACS Separator. The flow-through can be collected as the non-magnetic, unlabeled cell fraction.
Elution of the labeled cell fraction
The separation column is removed from the magnetic field and the retained cells are flushed out.
Both the labeled and unlabeled fractions can be recovered and used for downstream applications.
MACS Technology
MACS® Technology—the gold standard in cell separationMACS Technology is based on MACS MicroBeads, MACS
Columns, and MACS Separators—strong permanent magnets.
MicroBeads are superparamagnetic particles coupled to specific
monoclonal antibodies.
Target cells can be magnetically isolated by positive selection
using specific cell surface antigens or by depletion of unwanted
cells in order to obtain untouched cells. Furthermore, these two
separation strategies can easily be combined to provide greater
flexibility for the sequential sorting of complex subpopulations
of cells.
MACS MicroBeads
• Highly specific monoclonal antibody conjugates
• Small (50 nm), virus-sized nanoparticles
• Non-toxic, biodegradable
• Colloidal, for ease of handling and short incubation times
MACS Columns and MACS Separators
• Optimal recovery and high purity with MACS Columns
• Gentle to cells
• Automated cell separation with
autoMACS™ Pro Separator
MACS MicroBeads are nano-sized particles and are barely detectable by scanning electron microscopy. The micrograph shows a lymphocyte isolated by positive selection (left). Transmission electron micrograph of an isolated lymphocyte with MicroBeads (arrow) on the cell surface (right).
(Courtesy of Prof. Groscurth, Zürich, CH.)
Benefits of MACS® Technology at a glance:
• Fast—cell separation takes less than one hour
• Gentle—separated cells remain viable and functional
• Flexible—both labeled and unlabeled fractions can be
obtained with excellent purity and high recovery
• Easy separation of large cell numbers—up to 109
labeled cells per column
MidiMACS™ Separator autoMACS™ Pro Separator
MACS MicroBeads for indirect magnetic labeling
For maximum flexibility, indirect magnetic labeling with MACS
MicroBeads allows the use of any primary antibody. Monoclonal
or polyclonal primary antibodies can be either unconjugated,
biotinylated, or fluorochrome-conjugated.
5
MACS® Cell Separation Strategies
Sample preparationFor a broad range of tissues that are of interest for current tissue regeneration research,
the ideal cell sources have yet to be determined. Therefore, the isolation of stem cells from
embryonic, fetal, and adult sources is of great importance.
Preparing suitable suspensions of viable single cells from tissues is a critical step for
the successful isolation of tissue-specific stem cells. Miltenyi Biotec offers the new
gentleMACS™ Dissociator for the gentle and efficient dissociation of tissues. In addition, the
Neural Tissue Dissociation Kits, Pre-Separation Filters, and the Dead Cell Removal Kit allow
an optimal preparation of single-cell suspensions for subsequent separation.
gentleMACS™ DissociatorThe gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation
of tissues. Two types of unique gentleMACS Tubes are available for the instrument and
enable the time-saving and easy dissociation of tissues into single-cell suspensions or
homogenization of tissues to lysates in a closed system.
The range of protocols available for the gentle and efficient dissociation of tissues is
continuously expanding. Protocols are optimized to yield single-cell suspensions with high
viability rate. The gentleMACS Dissociator can further be used for the homogenization
of tissues or cells to extract biomolecules for molecular biology experiments, such as the
isolation of total RNA or mRNA.
gentleMACS Dissociator—features at a glance• Time-saving automated tissue dissociation or homogenization
• Standardized procedure
• Reliable and reproducible results
• High level of user safety
• Sterile sample handling
Cell separation and analysisFor research into future tissue regeneration applications, it is crucial to develop reliable
methods for the specific isolation of distinct stem and progenitor cell populations with
defined differentiation potential as well as their differentiated progenies.
Miltenyi Biotec offers numerous tools for the isolation of stem and progenitor cells
according to specific cell surface markers. For maximum flexibility, indirect magnetic
labeling with MACS MicroBeads allows the use of any primary antibody. Monoclonal or
polyclonal primary antibodies of choice can be either unconjugated, biotinylated, or
fluorochrome-conjugated. Furthermore, a large variety of products is available for the
analysis of stem and progenitor cells.
Human stem cells in tissue regeneration researchStem and progenitor cells have the potential to revolutionize tissue regeneration
and engineering in the future. To support research into the biology of stem
cells and to explore their extraordinary capacity for future tissue regeneration
applications, it is crucial to develop reliable methods for the specific isolation
of distinct stem and progenitor cell populations with defined differentiation
potential. Likewise, the isolation of their differentiated progenies is of immense
interest for research into future clinical applications.
For stem cell research, Miltenyi Biotec has developed numerous innovative
tools that allow straightforward sample preparation, cell separation, as well as
subsequent cell analysis.
The gentleMACS Dissociator—the gentle way of automated tissue dissociation
6
Human stem cells—sample preparation, cell separation and analysis
Sample preparation, cell separation, cell analysis
Embryonic stem cells and induced pluripotent stem cellsPluripotent embryonic stem cells (ESCs), derived from the inner cell mass of the blastocyst,
and induced pluripotent stem (iPS) cells are known for their capacity to differentiate into
virtually all cell types of the body and to self-renew while maintaining a stable karyotype.
Therefore, these cells are of special interest for basic tissue regeneration research to
understand developmental processes, and might provide the basis for clinical applications
in the future. For these applications it is crucial to use homogenous cell populations. MACS®
Technology enables the isolation of numerous particular cell types and subsets at high
purity—ready for in vitro and animal experiments.
Pluripotent cells express the surface markers SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, but not
CD15 (SSEA-1).1–3 The positive selection, for example, according to the expression of SSEA-4
using MACS MicroBeads for indirect magnetic labeling is a proven strategy for the isolation
of pluripotent cells.4,5 These markers can also be used for the depletion of pluripotent
cells from differentiated cultures, thereby reducing the risk of teratoma formation in
transplantation experiments.
After their in vitro differentiation, ESC- or iPS-derived cells exhibit a broad cellular
heterogeneity with respect to developmental stages and lineage specification. This leads
to highly heterogenous cell populations. Cell separation according to the expression of
certain surface markers can enrich target cells or deplete unwanted populations:
Hematopoietic stem cells (HSCs) can be isolated from differentiated ESCs by positive
selection according to the expression of, for example, CD34 or CD133.6–12
Endothelial progenitor cells (EPCs) can be separated from ESC cultures by using, for
example, the cell surface markers CD347,8,13 and CD31.
Early neural cell populations were enriched with FORSE-1 antibodies and MicroBeads
for indirect magnetic labeling. Various markers that indicate the developmental maturity
after neuronal differentiation have been described:
CD133+, CD271+, or CD146+ cells showed characteristics of neural stem or precursor cells,
A2B5+ cells showed characteristics of glial-restricted precursor cells, whereas CD56+, PSA-
NCAM+, or CD24+ cells showed characteristics of differentiated neurons.14
Cardiomyocytes might be separable from heterogenous ESC cultures according to
CD56+ expression.15
Cell separation
Cell analysis
microRNA expression profiling
Gene expression analysis
For more information on Miltenyi Biotec products for ESC and iPS cell research, please refer to the corresponding brochure that is available for download at www.miltenyibiotec.com.
7
Cell separation and analysis
Embryonic stem cells and induced pluripotent stem cells— separation and analysis
Mesenchymal stromal cellsMesenchymal stromal cells (MSCs) can be obtained from a variety of tissue sources
including bone marrow aspirate16,17, umbilical cord blood17, and even lipoaspirate18,19.
MSCs have shown the potential to diff erentiate into a variety of nonhematopoietic tissue
types, including bone or cartilage. Numerous cell surface antigens have been used for the
isolation of MSCs from various sources.
Bone marrowCD271 is a well-known marker for the isolation of MSCs from bone marrow.20–22
Marrow stromal antigen-1 (MSCA-1), which is recognized by clone W8B2, was shown to be
restricted to MSCs in the CD271bright population in bone marrow.22 These CD271brightCD45dim
MSCs show a higher clonogenic capacity compared to the CD271+CD45+ fraction.22
Therefore, MSCA-1 is a suitable marker for the identifi cation of MSCs with a high
proliferative potential.
Additional markers for the isolation of MSCs from bone marrow include CD117 23, CD105 24,25,
Stro-1 26 , CD146 27, and CD13328.
Multipotent adult progenitor cells (MAPCs) can be enriched from bone marrow by
depletion of CD235a (glycophorin A) and CD45.33
Cord bloodCD133 is a suitable marker for the identifi cation of MSCs from cord blood.17
LipoaspirateVarious strategies can be pursued for the isolation of MSCs from lipoaspirate: positive
selection of CD271+ cells 29 or CD146+ cells, positive selection of CD34+ cells30, as well as
depletion of CD45+ and CD31+ cells. 18,31
Dental pulpSTRO-1 and CD146 are appropriate markers for the separation of MSCs from dental pulp.27
Amniocentesis culturesMSC-like fetal-derived stem cells from amniocentesis cultures can be isolated according to
the expression of CD117 and share many markers of adult and embryonic stem cells. Fetal-
derived stem cells retain a multipotent ability to diff erentiate into cell types that represent
all embryonic germ layers. 32
Sample preparation
Cell separation
Cell analysis
Cell culture
Expression profi ling
MSCA-1 (W8B2)+ cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the MSC Research Tool Box – MSCA-1 (W8B2). Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC.
BM-MNCs before separation
MSCA-1+ cells
Anti-MSCA-1 (W8B2)-APC
CD
45-F
ITC
Anti-MSCA-1 (W8B2)-APC
CD
45-F
ITC
For more information on Miltenyi Biotec products for MSC research, please refer to the corresponding brochure available for download at www.miltenyibiotec.com.
8
Mesenchymal stromal cells—separation and analysis
Cell separation and analysis
Endothelial progenitor cells Regeneration of vascular tissue is an important topic in
therapeutic research, especially for the potential treatment of
peripheral vascular disease and the revascularization of ischemic
tissues, for example, in the heart.
Endothelial progenitor cells (EPCs) have been suggested to
play an important role in postnatal neoangiogenesis and
neovascularization. Therefore, EPCs have come into focus for
the potential treatment of ischemic or injured tissue and for the
coating of scaffolds to increase biocompatibility of biomaterials.
EPCs are defined by the expression of the markers CD34 and
CD309 (VEGFR-2/KDR). Analysis of CD133 expression allows the
distinction between early and matured EPCs in human.34,35 EPCs
were enriched according to their expression of CD34 or CD133
from different hematopoietic sources34–38,43. CD133+ cells have
been used in studies that show a significantly improved vascular
network restoration in an ischemic hind limb rat model38 and an
ischemic heart mouse model39. Furthermore, CD133+ cells were
used in combination with biodegradable scaffolds for the three-
dimensional tissue engineering of microvessels.40 Safety and
feasibility and, moreover, efficacy was shown in several clinical
trials investigating CD133+ cell therapy combined with coronary
artery bypass grafting41,42 and cell therapy alone43.
SPECT (single-photon emission computed tomographic) scan from the posterior wall area of a human heart. Courtesy of Prof. Gustav Steinhoff, Rostock, Germany.
9
Endothelial progenitor cells—separation and analysis
Tissue-resident stem cells have been found in almost any tissue.
A better understanding of their biology and the development
of methods for their isolation and expansion might therefore be
useful for tissue regeneration. In addition, stem cells from the
hematopoietic system have been described to contribute to the
regeneration of tissues. Following, a few examples for research
on the regeneration of certain tissues are presented.
Liver tissueIn contrast to other organs, the liver is known for its capacity
to regenerate in situ. This is due to the population of stem cells
within the liver. These stem cells have been successfully isolated
using MACS® Technology by targeting CD326 (EpCAM)44,45,
CD13345, or CD117.46 The transplantation of bone marrow–derived
CD133+ cells has been shown to benefit the expansion of liver
tissue in situ prior to partial hepatectomy.47
Muscle tissue The identification and isolation of muscle stem and progenitor
cells by the markers CD13348 and CD5649,50, respectively, is of
importance for research on smooth, skeletal, and even cardiac
muscle tissue regeneration. After transplantation into mouse
models, human CD133+ stem cells from peripheral blood and
circulating endothelial progenitor cells (cEPCs) from umbilical
cord blood were capable of regenerating in situ dystrophic
muscle tissue51 and skeletal muscle52, respectively. Cardiac
muscle regeneration is a prime goal in cardiovascular disease
research; functional cardiomyocytes can be generated in vitro
from ESCs15, CD133+ fetal liver cells53, and from CD34+ cEPCs from
peripheral blood54.
Tissue regeneration research—examples
CD133+ cells, isolated from mobilized peripheral blood, gave rise to adherent cells after 3–5 weeks of cultivation. These cells were able to differentiate into hepatocyte-like cells. (A) The cells are stained for hepatocyte nuclear factor-3 (FITC), albumin (Cy3), and nuclei (DAPI). (B) The cells are stained for cytokeratin 19 (Cy3) and nuclei (DAPI) (200×). (Courtesy of Selim Kuçi, Tübingen, Germany.)
CD133+ cells isolated from mobilized PBMCs were cultivated for 3–5 weeks. Adherent cells were able to differentiate into skeletal muscle–like cells. The cells are stained for desmin (FITC), actin (Cy3), and nuclei (DAPI)(200×). (Courtesy of Selim Kuçi, Tübingen, Germany.)
A
B
10
Tissue regeneration research
Neural tissueThe regeneration of neural tissue has far-reaching consequences
for the potential treatment of debilitating neurodegenerative
diseases or injuries, including stroke and spinal cord damage.
Human neural stem cells that were isolated from fetal brain
according to CD133 expression have been shown to diff erentiate
in vitro and in vivo into cells with neural phenotypes and even
restore the function of damaged spinal tissue in mice.55
Furthermore, CD133+ cells isolated from mobilized peripheral
blood56 or skin57 can be diff erentiated into neural lineages.
Neuronal-committed precursors from mammalian brain can be
magnetically isolated for research purposes via the depletion
of A2B5+ cells followed by the positive selection of PSA-NCAM+
cells.58
Tissue regeneration research—examples
B
A
C
CD133+ cells isolated from mobilized PBMCs were cultivated for 3–5 weeks. Adherent cells were able to differentiate into neural-like cells. (A) Astrocyte-like cells stained for GFAP (Cy3), EPO (FITC), and nuclei (DAPI). (B) Oligodendrocyte-like cells stained with GFAP (FITC), MBP (Cy3), and DAPI (nuclei). (C) Neuronal-like cells stained for betatubulin III (Cy3) and nuclei (DAPI)(200×). (Courtesy of Selim Kuçi, Tübingen, Germany.)
For more information on Miltenyi Biotec products for neuroscience research, please refer to the corresponding brochure that is available for download at www.miltenyibiotec.com.
11
MACS® Technology
1. Kaufman, D. S. et al. (2001) Proc. Natl. Acad. Sci. USA 98: 10716–10721.2. Chang, K. et al. (2006) Blood 108: 1515–1523.3. Carpenter, M. K. et al. (2003) Cloning Stem Cells 5: 79–88.4. Cheng, L. et al. (2003) Stem Cells 21: 131–42. 5. Schulz, T. C. et al. (2004) Stem Cells 22: 1218–1238.6. Bandi, S. and Akkina, R. (2008) AIDS Res. Ther. 5: 1.7. Wang, Z. Z. et al. (2007) Nat. Biotechnol. 25: 317–318.8. Chen, T. et al. (2007) Stem Cells 25: 392–401.9. Narayan, D. A. et al. (2006) Blood 107: 2180–2183.10. Galic, Z. et al. (2006) Proc. Natl. Acad. Sci. USA 103: 11742–11747.11. Kaufman, D. S. et al. (2001) Proc. Natl. Acad. Sci. USA 98: 10716–10717.12. Vodyanik, M. A. et al. (2006) Blood 108: 2095–2105. 13. Ferreira, L. S. et al. (2007) Circ. Res. 101: 286–294.14. Pruszak, J. et al. (2007) Stem Cells 25: 2257–2268.15. Xu, S. et al. (2006) Stem Cells and Dev. 15: 631–639.16. Jones, E. A et al. (2002) Arthritis Rheum. 46: 3349–3360.17. Tondreau, T. et al. (2005) Stem Cells 23: 1105–1112.18. Boquest, A. C. et al. (2005) Mol. Biol. Cell 16: 1131–1141.19. Meyerrose, T. E. et al. (2007) Stem Cells 25: 220–227.20. Jones, E. A. et al. (2006) Cytometry B Clin. Cytom. 70: 391–399.21. Quirici, N. et al. (2002) Exp. Hematol. 30: 783–791.22. Bühring, H. J. et al. (2007) Ann. NY Acad. Sci. 1106: 262–271.23. Huss, R. and Moosmann, S. (2002) Br. J. Hematol. 118: 305–312.24. Aslan, H. et al. (2006) Stem Cells 24: 1728–1737.25. Majumdar, M. K. et al. (2003) J. Biomed. Sci. 10: 228–24.26. Gronthos, S. et al. (1994) Blood 84: 4164–4173.
27. Shi, S. and Gronthos, S. (2003) J. Bone Miner. Res. 18: 696–704.28. Pozzobon, M. et al. (2008) Stem Cells and Dev. (in press).29. Godthardt, K. (2007) MSC 2007 Adult Mesenchymal Stem Cells in Regenerative Medicine, Cleveland, Poster Nr. 301, Session III.30. Astori, G. et al. (2007) J. Transl. Med. 5: 55.31. Noer, A. et al. (2006) Mol. Biol. Cell 17: 3543–3556.32. De Coppi, P. et al. (2007) Nat. Biotechnol. 25: 100–106.33. Reyes, M. et al. (2002) J. Clin. Invest. 109 : 337–346.34. Peichev, M. et al. (2000) Blood 95: 952–958.35. Rafii, S. and Lyden, D. (2003) Nat. Med. 9: 702–712.36. Gehling, U. et al. (2000) Blood 95: 3106–3112.37. Taguchi, A. et al. (2004) J. Clin. Invest. 330–338.38. Suuronen, E. et al. (2006) Circulation. 114 (suppl. 1): 138–144.39. Ma, N. et al. (2006) Cardiovasc. Res. 71: 158–169.40. Wu, X. et al. (2004) Am. J. Physiol. Heart. Circ. Physiol. 287: H480–H487.41. Stamm, C. et al. (2004) Thorac. Cardiovasc. Surg. 52: 152–158.42. Stamm, C. et al. (2007) J. Thorac. Cardiovasc. Surg. 133: 717–725.43. Klein, H. M. et al. (2007) Heart Surg. Forum 10: E66–69.44. Schmelzer, E. et al.(2007) J. Exp. Med. 204: 1973–1987.45. Schmelzer, E. et al. (2006) Stem Cells 24: 1852–1858.46. Laurson, J. et al. (2005) Int. J. Exp. Pathol. 86: 1–18.47. Schulte am Esch, J. et al. (2005) Stem Cells 23: 463–470.48. Alessandri, G. et al. (2004) Lancet 364: 1872–1883.49. Sinanan, A. et al. (2004) Biotechnol. Appl. Biochem. 40: 25–34.50. De Luna, N. et al. (2006) J. Biol. Chem. 281: 17092–17098.51. Torrente, Y. et al. (2004) J. Clin. Invest. 114: 182–195.52. Pesce, M. et al. (2003) Circ. Res. 93: 51–62.53. Schmelkov, S. V. et al. (2005) Circulation 111: 1175–1183.54. Badorff, C. et al. (2003) Circulation 107: 1024–1032.55. Cummings, B. J. et al. (2005) Proc. Natl. Acad. Sci. USA 102: 14069–14074.56. Kuçi, S. et al. (2003) MACS&more 7/1: 6–8.57. Belicchi, M. et al. (2004) J. Neurosci. Res. 77: 475–486.58. Seidenfaden, R. et al. (2006) Mol. Cell Neurosci. 32: 187–198.
References
12
References
13
MACS® Products
MACS® Products for sample preparation, cell separation, and cell analysis
Product Components or capacity Order no.
gentleMACS™ Starting Kit
gentleMACS™ Dissociator C Tubes, 25 pieces M Tubes , 25 piecesPower cord User manual gentleMACS Protocols
130-093-235
C Tubes C Tubes, 25 pieces, single-packed
130-093-237
M Tubes M Tubes, 25 pieces, single-packed M Tubes, 50 pieces per bag
130-093-236 130-093-458
Neural Tissue Dissociation Kit (P)
50 tests with up to 400 mg of neural tissue
130-092-628
Neural Tissue Dissociation Kit (T)
50 tests with up to 400 mg of neural tissue
130-093-231
Pre-Separation Filters
50 filters 130-041-407
Dead Cell Removal Kit
For 10⁹ total cells 130-090-101
Product Order no.
Anti-A2B5 MicroBeads, human, mouse, rat 130-093-388
MSC Research Tool Box – MSCA-1 (W8B2), human
130-093-572
Anti-MSCA-1 (W8B2) MicroBead Kit, human 130-093-583
Anti-PSA-NCAM MicroBeads, human, mouse, rat
130-092-966
CD31 MicroBead Kit, human 130-091-935
CD34 MicroBead Kit, human 130-046-702
CD34 MultiSort Kit, human 130-056-701
CD45 MicroBeads, human 130-045-801
CD56 MicroBeads, human 130-050-401
CD105 MicroBeads, human 130-051-201
CD117 MicroBead Kit, human 130-091-332
CD133 MicroBead Kit, human 130-050-801
CD146 MicroBead Kit, human 130-093-596
CD235a (Glycophorin A) MicroBeads, human 130-050-501
MSC Research Tool Box – CD271 (PE), human 130-092-867
MSC Research Tool Box – CD271 (APC), human 130-092-291
CD271 MicroBead Kit (PE), human 130-092-819
Lineage Cell Depletion Kit, human 130-092-211
CD271 MicroBead Kit (APC), human 130-092-283
CD326 (EpCAM) MicroBeads, human 130-061-101
MACS Products for the analysis of stem and progenitor cells
Product Order no.
Anti-A2B5-PE, -APC, pure, human, mouse, rat
130-093-581, 130-093-582, 130-092-394
Anti-MSCA-1 (W8B2)-FITC, -PE, -APC, -Biotin, pure, human
130-093-585, 130-093-587, 130-093-589, 130-093-593, 130-093-595
Anti-PSA-NCAM-PE, -APC, human, mouse, rat
130-093-274, 130-093-273
CD31-FITC, -PE, -APC, human 130-092-64, 130-092-653, 130-092-652
CD34-FITC, -PE, -APC, human 130-081-001, 130-081-002, 130-090-954
CD56-PE, APC, pure, human 130-090-755, 130-090-843, 130-090-955
CD117 (A3C6E2)-PE, -APC, human 130-091-734, 130-091-733
CD117 (AC126)-PE, human 130-091-735
CD133/1 (AC133)-PE, -APC, -Biotin, pure, human
130-080-801, 130-090-826, 130-090-664, 130-090-422
CD133/2 (293C3)-PE, -APC, -Biotin, pure, human
130-090-853, 130-090-854, 130-090-852, 130-090-851
CD133/1 (W6B3C1) pure, human 130-092-395
CD146-FITC, -PE, -APC, -Biotin, pure, human
130-092-851, 130-092-853, 130-092-849, 130-092-852, 130-092-850
CD271 (LNGFR), -PE, -APC, -Biotin, human
130-091-885, 130-091-884, 130-091-883
CD309 (VEGFR/KDR)-PE, -APC, -Biotin, human
Coming soon
CD326 (EpCAM)-FITC, -PE, -APC, human
130-080-301, 130-091-253, 130-091-254
EPC Enrichment and Enumeration Kit, human
130-093-477
MACS Products for sample preparation
Product Order no.
Anti-FITC MicroBeads 130-048-701
Anti-PE MicroBeads 130-048-801
Anti-APC MicroBeads 130-090-855
Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads 130-091-395
Anti-Cy7 MicroBeads 130-091-652
Anti-Biotin MicroBeads 130-090-485
Streptavidin MicroBeads 130-048-101
Rat Anti-Mouse IgG1 MicroBeads 130-047-101
Rat Anti-Mouse IgG2a+b MicroBeads 130-047-201
Rat Anti-Mouse IgM MicroBeads 130-047-301
Goat Anti-Mouse IgG MicroBeads 130-048-401
Mouse Anti-Rat Kappa MicroBeads 130-047-401
Goat Anti-Rat IgG MicroBeads 130-048-501
Goat Anti-Rabbit IgG MicroBeads 130-048-602
MACS Products for indirect magnetic labeling
MACS Products for the isolation of stem and progenitor cells
MACS® Technology
MACS® Products for cell culture
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NH media for the enumeration, expansion, and differentiation of MSCsMSCs are of great importance to researchers working towards the development of novel tissue regeneration therapies. However, in order to properly evaluate the potential of MSCs completely, it is crucial to establish standardized and reproducible procedures for stem cell isolation and cultivation. MACS® NH Stem Cell Media are optimized for the most convenient enumeration and expansion of nonhematopoietic (NH) stem cells from a variety of sources, including bone marrow aspirate, lipoaspirate, or potentially any tissue or organ within the human body. Miltenyi Biotec also offers media to evaluate the differentiation potential of NH stem cells during in vitro cultivation. Media are available for the reliable and reproducible differentiation of NH stem cells to adipocyte, chondrocyte, or osteoblast lineages. These media can also be used to define the full differentation capacity of an NH stem cell population: MSCs must be able to form all three cell lineages.
AdipocytesNH AdipoDiff Medium
ChondrocytesNH ChondroDiff Medium
OsteoblastsNH OsteoDiff Medium
MACS® NH Media enable the differentiation of mesenchymal stromal cells (MSCs) into functional nonhematopoietic (NH) cell types.
MSC expansionNH Expansion Medium
MSC enumerationNH CFU-F Medium
NH stem cell source, e.g., bone marrow, lipoaspirate
Basic media and cytokines for stem cell cultureMiltenyi Biotec offers cell culture media for a broad spectrum of applications. In addition, high-quality recombinant cytokines and growth factors are available, that are well-suited for various applications such as cell culture, differentiation studies, and biological assays. Selected products are available in a premium-grade format with excellent purity and high, well-defined activity as well as in research-grade quality. For a complete list of available cytokines, please visit http://www.miltenyibiotec.com/cytokines.
CytoMix – MSC, humanThe CytoMix – MSC, human (130-093-552) is a composition of cytokines for the most efficient and reproducible expansion of human MSCs. In combination with the NH Expansion Medium, CytoMix – MSC optimally supports the proliferation of human MSCs, especially after separation, e.g., according to CD271 or MSCA-1 (W8B2) expression using MACS® Technology.
MACS NH Media Order no.
NH CFU-F Medium 24×5 mL 130-091-676
NH Expansion Medium 500 mL 130-091-680
NH AdipoDiff Medium 100 mL 130-091-677
NH ChondroDiff Medium 100 mL 130-091-679
NH OsteoDiff Medium 100 mL 130-091-678
MACS Basic Culture Media Order no.
DMEM 500 mL 130-091-437
DMEM with stable glutamine 500 mL 130-091-438
RPMI 1640 500 mL 130-091-440
RPMI 1640 with stable glutamine 500 mL 130-091-439
MACS Cytokines and Growth Factors
Human: BDNF; BMP-2; EGF; EG-VEGF; FGF-2; FGF-4; Flt3-Ligand; G-CSF; GM-CSF; HGF; IL-3; IL-6; IL-11; PDGF-AA; PDGF-AB; PDGF-BB; SCF; SDF-1α; TGF-α; TGF-β1; TGF-β3; TNF-α; VEGF (121 aa); VEGF (165 aa)
Mouse: EGF; Flt3-Ligand, G-CSF; GM-CSF; IL-3 (135 aa); IL-6; SCF; TNF-α; VEGF (164 aa); VEGF (165 aa);
Rat: VEGF-C; VEGF-C (C152S)
MACS® Products
Molecular biology products and services
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Stem cell differentiation tracking by gene expression profilingMACSmolecular provides a highly innovative range of products and services with a strong focus on gene expression profiling. Particularly when isolating stem cells, sensitive downstream analyses are required.
One-step mRNA isolation and in-column cDNA synthesis Premium mRNA is isolated within 15 minutes directly from cells or tissues. The µMACS™ One-step cDNA Kit combines efficient magnetic isolation of mRNA with revolutionary in-column cDNA synthesis. Purified cDNA can be generated from just a few to as many as 10⁷ cells.
PIQOR™ Stem Cell MicroarrayThe PIQOR™ Stem Cell Microarray comprises 942 relevant marker genes for human stem cells and their differentiation. It is available as a convenient microarray kit* or within the scope of the microarray service**. Gene expression experiments allow for the quality control of different stem cell types, comparison between different stages of differentiation, as well as the optimization of differentiation protocols.
SuperAmp™ Service ***When the number of stem cells for analysis is low, Miltenyi Biotec offers the ideal solution for gene expression profiling needs. The SuperAmp™ Service (available as an extension of the PIQOR™ Microarray Service) can reliably amplify mRNA million-fold from as little as one cell. The service is ideal for stem cells sorted with MACS® Technology, flow cytometry, or even from laser capture microdissected tissue.
miRXplore™ Kits and ServicesExplore microRNA expression in human and mouse stem cells with the new miRXplore™ Microarray Kits and Services. Designed in collaboration with experts at the Rockefeller University¹, the microarray covers more than 2700 human, mouse, rat, and viral microRNA sequences and possess rigorous internal control system. Sequences differing by just one oligonucleotide can be reproducibly detected and re-ratios calculated with the use of the proprietary miRXplore Universal Reference.
Reference1. Landgraf, P. et al. (2007) Cell 129: 1401–1414.
a-Hyb™ Hybridization Station
* PIQOR™ Microarray Kits are not available in the US and Canada.** Microarray Service includes all experimental steps from RNA isolation to
primary data analysis. Final data are returned including an extensive written report. Further Bioinformatics Services, such as pathway or cluster analysis, are also available.
*** In combination with the Microarray Services only. The SuperAmp Service is not available for microRNA amplification
mRNA isolation/cDNA synthesis
µMACS mRNA Isolation Kit Small ScaleLarge ScaleFor Total RNA
130-075-201130-075-101130-075-102
µMACS mRNA Isolation Starting Kit 130-075-202
µMACS One-step cDNA Kit 130-091-902
µMACS One-step cDNA Starting Kit 130-091-989
PIQOR Microarray Kit *
PIQOR Stem Cell Microarray Kit, antisense
4 Microarrays 130-092-033
8 Microarrays 130-092-034
PIQOR Microarray Service **
Service Stem Cell Microarray Plus Amplification 160-000-765
SuperAmp Amplification ***
SuperAmp Service (per sample) 160-000-936
miRXplore Microarray Kit
4 Microarrays 130-093-254
8 Microarrays 130-093-272
miRXplore Microarray Services
miRXplore Microarray Service 160-001-143
miRXplore Universal Reference Service 160-001-161
miRXplore Additional Total RNA Extraction 160-001-162
Miltenyi BiotecMiltenyi Biotec was founded in 1989; nowadays, more than 1100 employees develop, produce, and sell innovative products for cell research and clinical applications. Due to an intense focus on stem cell research at Miltenyi Biotec, the portfolio includes the largest range of products available for the separation of stem cells. Moreover, Miltenyi Biotec offers integrated solutions for a wide variety of research needs—from sample preparation to molecular analysis.
Miltenyi Biotec Asia Pacific Pte. Ltd. (Singapore)Phone +65 6238 [email protected]
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Miltenyi Biotec Ltd. (UK)Phone +44 1483 799 800 [email protected]
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Miltenyi Biotec Trading (Shanghai) Co., Ltd. (P.R. China) Phone +86 21 6235 [email protected]
Miltenyi Biotec Inc. 12740 Earhart AvenueAuburn, CA 95602, USAPhone 800 FOR MACS, +1 530 888 8871 Fax +1 530 888 [email protected]
Miltenyi Biotec Australia Pty. Ltd.Phone +61 02 8877 [email protected]
Miltenyi Biotec GmbH Friedrich-Ebert-Straße 6851429 Bergisch Gladbach GermanyPhone +49 2204 8306-0Fax +49 2204 [email protected]
www.miltenyibiotec.com
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS is a registered trademark of Miltenyi Biotec GmbH. a-Hyb, μMACS, MidiMACS, autoMACS, gentleMACS, MACSQuant, miRXplore, PIQOR, and SuperAmp are trademarks of Miltenyi Biotec GmbH. Copyright © 2008 Miltenyi Biotec GmbH. All rights reserved.
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