This Monthly Progress Report provides a summary of JBEI’s ... · PDF fileThis Monthly Progress Report provides a ... Sr. Business Editor from Chemical & Engineering News gave a seminar

DOE Bioenergy Research Center (BRC) - Joint BioEnergy Institute

Monthly Progress Report – August 2015

Fiscal Year: 2015

Year / Period: Year 8 / Month 11 / August 1 - 31, 2015

Department of Energy’s Joint BioEnergy Institute is supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through Contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy.

JBEI contacts Jay Keasling, Chief Executive Officer, [email protected], 510-495-2620 Nick Everson, Chief Operating Officer, [email protected], 510-486-6268

Executive Summary This Monthly Progress Report provides a summary of JBEI’s activities for the month of August 2015, eleventh month of fiscal year 2015:

Item Count Highlights

Publications in Print 8 "The DOE Bioenergy Research Centers: History, Operations, and Scientific Output". BioEnergy Research

Invention Disclosures 3 “Knockdown of a Lipid-transfer Protein for Enhancing Drought Tolerance and Increasing the Hexose/Pentose Cell Wall Sugar Ratio in Arabidopsis”

Media Citations 3 JBEI’s Ionic Liquid research was featured on Biofuels Digest and Downstream Business.

Awards 1 Nathan Hillson as a member of the FutureBio team was awarded the Trailblazer Award by NSF Bay Area Regional I-Corps Summer 2015 program.

Outreach Interactions Education: 12, Public: 6 and Scientific Community: 10

On-Site Seminars 6 Melody Bomgardner, Sr. Business Editor from Chemical & Engineering News gave a seminar on “Communicating Science, or how to get people to care about what you do”.

Industry Interactions 38 Interactions with Google, Illium and The Earth Partners.

FY15 Milestones Milestones completed:

- Feedstocks: Characterization of novel nucleotide sugar transporters

- Feedstocks: Analysis of stacked traits in Arabidopsis

- Deconstruction: Design, synthesis and assessment of lignin and hemicellulose derived IL mixtures for mixed and engineered feedstock pretreatment

- Deconstruction: Identify five bacterial strains capable of metabolizing mixtures of aromatics from pretreatment-derived lignin streams

- Fuels Synthesis: Improve overall xylose utilization capability in S. cerevisiae strains and examine performance in industrially relevant yeast strains

- Fuels Synthesis: E. coli membrane editing: With JGI, generate 10-20 single and double mutants and test improvement in expression of beneficial tolerance pump and corresponding tolerance to toxic fuels

mailto:[email protected]


DOE Bioenergy Research Center (BRC) - Joint BioEnergy Institute

Monthly Progress Report – August 2015

Fiscal Year: 2015

Year / Period: Year 8 / Month 11 / August 1 - 31, 2015

Department of Energy’s Joint BioEnergy Institute is supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through Contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy.

JBEI contacts Jay Keasling, Chief Executive Officer, [email protected], 510-495-2620 Nick Everson, Chief Operating Officer, [email protected], 510-486-6268

- Technology: Support the optimization of T and IL tolerant cellulose/hemicellulase cocktail using HT-NIMS

- Technology: Implement software to automate unit operations (e.g., droplet dispensing, routing, merger, and sorting) on an integrated droplet chip

- Technology: Implement membrane proteomics assays to enable E. coli membrane editing with Fuels Synthesis Division

- Technology: Develop absolute quantification targeted proteomic assays for 150 S. cerevisiae proteins

- Technology: Develop rapid untargeted proteomics methods for relative quantification of >800 E. coli and S. cerevisiae proteins

- Industry: Generate >10 inventions

- Industry: Hold an annual meeting of the IAC to inform JBEI research of industry concerns and technology bottlenecks.

- Operations: Hold the annual meeting and report to DOE on the findings of the committee

Milestone delayed:

- Technology: Structural characterization of Fatty Acid Synthase and other large complexes by single particle cryo electron microscopy

Milestones extended:

- Feedstocks: Heterologous screening of transferases to promote in planta design of polysaccharides

- Feedstocks: Transgenic plants with dominant repressors of hemicellulose biosynthesis characterized (CWB)



Dashboard - August 2015 - DOE

FY 2015 Dashboard For Period: August 1 - 31, 2015

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Publications

In Print

Johnson, R. E., de Rond, T., Lindsay, V. N., Keasling, J. D., & Sarpong, R. (2015). "Synthesis of Cycloprodigiosin Identifies the Natural Isolate as a Scalemic Mixture". Org Lett, 17(14), 3474-3477. doi, 10.1021/acs.orglett.5b01527 http://www.ncbi.nlm.nih.gov/pubmed/26114660

Klein-Marcuschamer, D., & Blanch, H. W. (2015). "Renewable fuels from biomass: Technical hurdles and economic assessment of biological routes". Aiche Journal, 61(9), 2689-2701. doi, 10.1002/aic.14755 http://onlinelibrary.wiley.com/doi/10.1002/aic.14755/abstract

Nafisi, M., Stranne, M., Fimognari, L., Atwell, S., Martens, H. J., Pedas, P. R., Hansen, S. F., Nawrath, C., Scheller, H. V., Kliebenstein, D. J., & Sakuragi, Y. (2015). "Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses". [Original Research]. Front Plant Sci, 6, 550. doi, 10.3389/fpls.2015.00550 http://www.ncbi.nlm.nih.gov/pubmed/26257757

Poust, S., Piety, J., Bar-Even, A., Louw, C., Baker, D., Keasling, J. D., & Siegel, J. B. (2015). "Mechanistic Analysis of an Engineered Enzyme that Catalyzes the Formose Reaction". Chembiochem. doi, 10.1002/cbic.201500228 http://www.ncbi.nlm.nih.gov/pubmed/26109266

Sathitsuksanoh, N., Sawant, M., Truong, Q., Tan, J., Canlas, C. G., Sun, N., Zhang, W., Renneckar, S., Prasomsri, T., Shi, J., Çetinkol, Ö., Singh, S., Simmons, B. A., & George, A. (2015). "How Alkyl Chain Length of Alcohols Affects Lignin Fractionation and Ionic Liquid Recycle During Lignocellulose Pretreatment". BioEnergy Research, 1-9. doi, 10.1007/s12155-015-9643-9 http://dx.doi.org/10.1007/s12155-015-9643-9

Sharma, M. K., Sharma, R., Cao, P., Harkenrider, M., Jenkins, J., Grimwood, J., Zhang, J., Udvardi, M. K., Schmutz, J., & Ronald, P. C. (2015). "Targeted Switchgrass BAC Library Screening and Sequence Analysis Identifies Predicted Biomass and Stress Response-Related Genes". BioEnergy Research, 1-14. doi, 10.1007/s12155-015-9667-1 http://dx.doi.org/10.1007/s12155-015-9667-1

Slater, S. C., Simmons, B. A., Rogers, T. S., Phillips, M. F., Nordahl, K., & Davison, B. H. (2015). "The DOE Bioenergy Research Centers: History, Operations, and Scientific Output". BioEnergy Research, 1-16. doi, 10.1007/s12155-015-9660-8 http://dx.doi.org/10.1007/s12155-015-9660-8

Smith-Moritz, A. M., Hao, Z., Fernández-Niño, S. G., Fangel, J. U., Verhertbruggen, Y., Holman, H.-Y. N., Willats, W. G. T., Ronald, P. C., Scheller, H. V., Heazlewood, J. L., & Vega-Sánchez, M. E. (2015). "Structural characterization of a mixed-linkage glucan deficient mutant reveals alteration in cellulose microfibril orientation in rice coleoptile mesophyll cell walls". [Original Research]. Frontiers in Plant Science, 6. doi, 10.3389/fpls.2015.00628 http://www.frontiersin.org/Journal/Abstract.aspx?s=1294&name=plant_biophysics_and_modeling&ART_DOI=10.3389/fpls.2015.00628



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Media Citations

Date Article Source

Aug 3 Century-Old Rice Blight Finally Toppled? Key to Immunity Discovered Nature World News

Aug 16 Ionic Liquid Pretreatment: The Digest’s 2015 8-Slide Guide Biofuels Digest

Aug 28 Ionic-Liquid Pretreatment Technologies Offered For License Downstream Business

Awards

Name Award

Nathan Hillson NSF Bay Area Regional I-Corps Summer 2015, Trailblazer Award

Outreach – Education

Date Presenters Audience or Presentation Title Activity Location

Aug 4 Philip Gach Laney College Tour On-site

Aug 5 Makeda Nuruddin Biotech Partners Tour On-site

Aug 6 Garima Goyal, Sebastian Kolinko and Steve Shih

National Student Leadership Conference Tour On-site

Invention Disclosures Inventor(s)

2015-153 - “Coenzyme A Ligase for Cinnamates, Benzoates, and 3-Phenylpropionates” Aymerick Eudes and Dominique Loque

2015-156 - “A Method to Optimize Expression of Synthetic Promoters in Eukaryotic Systems” Dominique Loque and Patrick Shih

2015-158 - “Knockdown of a Lipid-transfer Protein for Enhancing Drought Tolerance and Increasing the Hexose/Pentose Cell Wall Sugar Ratio in Arabidopsis”

Henrik Vibe Scheller, Rhea Stoppel and Virginia Tartaglio



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Aug 6 Leo d'Espaux, Kamrun Zargar and Sarah Rodriguez

National Student Leadership Conference Tour On-site

Aug 7 Mi Yeon Lee Chuangnam National University Tour On-site

Aug 8 Jesus Barajas MIT Tour On-site

Aug 10 Belle Smith University Professionals and Technical Employees

Tour On-site

Aug 12 Jay Keasling and Nathan Hillson

Paul G. Allen Family Foundation Tour On-site

Aug 13 Belle Smith, CJ Joshua, Dominique Loque and Irina Silva

BRAVO! Biotech Partners Intern Celebration Event Off-site

Aug 18 Irina Silva and Peter Matlock

Digital World Biology Other On-site

Aug 20 Aaron Socha University of Hannover - Germany Tour On-site

Aug 24 Anna Lechner SIO, UCSD Tour On-site

Outreach – Public


Aug 8 Belle Smith, Dominique Loque and Virginia Tartaglio

Chabot Space & Science Center: Building with Biology

Event Chabot Space & Science Center

Aug 12 Jay Keasling and Nathan Hillson

Senate Energy and Water Development Appropriations

Tour On-site



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Aug 11 Irina Silva, Kelly Owen and Lida Gifford

BETO BioComms Group Seminar Webinar

Aug 15 Belle Smith, Dominique Loque, Nathan Hillson and Virginia Tartaglio

Chabot Space & Science Center: Building with Biology

Event Chabot Space & Science Center

Aug 26 Harry Beller, Irina Silva, Jay Keasling, Jenny Mortimer, Nathan Hillson, Paul Adams, Peter Matlock, Steven Singer and Taek Soon Lee

C&E News Meeting, Seminar and Tour

On-site

Aug 31 Blake Simmons, Henrik Scheller, Paul Adams and Sarah Richardson

Congressman Mark DeSaulnier Tour On-site

Outreach – Scientific Community


Aug 1-7 Alex Yi-Lin Tsai, George Wang, Edward E. K. Baidoo, Aymerick Eudes, Chengcheng Zhang, Dominique Loque, Jay D. Keasling, Laura E. Bartley, Henrik V. Scheller.

Cell Wall Biosynthesis in Grasses: Identification of Feruloyl-Coenzyme A: Monolignol Acyltransferase Activity of Oryza sativa AT5

Presentation Gordon Research Conference. Andover, NH

Aug 3 Steven W. Singer A Community Approach to Identifying New Cellulases

Presentation Society for Industrial Microbiology Annual Meeting, Philadelphia, PA



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Aug 3 Hector Garcia Martin Principal Component Analysis of Proteomics (PCAP) as a tool to direct metabolic engineering

Presentation SIMB 2015, Philadelphia, PA

Aug 14 Alex Yi-Lin Tsai JBEI Overview Presentation University of Toronto, Toronto, Canada

Aug 16-20 Jian Sun, Jian Shi, Tanmoy Dutta, Blake Simmons, Seema Singh

Pervaporation membrane for the efficient recovery of ionic liquid from mixed lignocellulosic feedstock processing

Presentation 250th American Chemical Society National Meeting & Exposition, Boston, MA

Aug 16-20 Heather Parker, Tanmoy Dutta, Seema Singh, Blake Simmons, Matthew Jones, Christopher Chuck

Catalytic deconstruction of model lignin compounds in ionic liquids


Aug 16-20 Tanmoy Dutta, Eileen Wang, Ramakrishnan Parthasarathi, Nancy Isern, Jian Sun, Rosalie Chu, Nikola Tolic, John Cort, Blake Simmons, Seema Singh

Mechanistic study of lignin depolymerization in ionic liquids


Aug 16-20 Ramakrishnan Parthasarathi, Tanmoy Dutta, Jian Sun, Blake Simmons, Seema Singh

Biomass dissolution and dissociation in acidic and basic ionic liquids: A quantum chemical study


Aug 19 Oge Nnadi Inventory of Composable Elements (ICE) ACS Synthetic Biology Workflow Update and SBOL XML/RDF upload/download

Presentation WBDA 2015, Live demos of SBOL-based tools session, Seattle, WA



8 / 22

Aug 25-27 Blake Simmons Development of advanced biofuels and biomass conversion technologies at the Joint BioEnergy Institute

Presentation International Congress and Expo on Biofuels and Bioenergy, Valencia, Spain



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Onsite Seminars

Date Type Presenter Title Affiliation

Aug 5 JBEI Seminar Chijioke Joshua Lignin deconstruction and recalcitrance: Re-philosophizing the paradigm

Microbial Communities, Deconstruction Division

Aug 12 General Seminar

Feedstocks Guest

Prof. Debra Mohnen

Moving the Wall: revisiting plant cell wall models based on phenotypes of mutant and transgenic plants affected in matrix polysaccharide biosynthesis

BESC, Professor of Biochemistry & Molecular Biology, The University of Georgia, Complex Carbohydrate Research Center


Fuels Synthesis Guest

David Ando Physical Modeling of Disordered Protein Assemblies

UC Merced


Deconstruction Guest

Gigi Gronvall Mitigating the Risks of Synthetic Biology

UPMC Center for Health Security


Technology/Commercialization Guest

Melody Bomgardner, Sr. Business Editor

Communicating Science, or how to get people to care about what you do

Chemical & Engineering News

Aug 27 General Seminar Fuels Guest Jason Bouvier Functional Discovery in the Oxidative D-Galacturonate Assimilation Pathway by Agrobacterium tumefaciens and Ralstonia pickettii

University of Illinois at Urbana-Champaign



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Industry Interactions

Date Company / Contact

Aug 7, Aug 19

Ajinomoto / Erika Yoshida

Aug 9 NY Times / Amy Harmon

Aug 10 Monsanto / Ginni Ursin

Aug 10 Marrone Bio Innovations / Phyllis Himmel

Aug 10 Dow / Steve Hahn, Leigh Thompson

Aug 10 DuPont / Luis Cascao-Pereira, Karl Sanford, Nathan Danielson

Aug 10 Ambercycle / Akshay Sethi

Aug 10 Elemental Energy LLC / Clifford Black

Aug 11 Chevron / Jeffrey Jacobs, Winnie Lieu, Doug Spooner

Aug 11 Technology Holding / Mukund Karanjikar and Pallavi Chitta

Aug 11 Ilium LLC / Aaron Socha

Aug 11 Ceres / Roger Pennell

Aug 12 China Lake Naval Weapons Center / Bill Hogan

Aug 12 Chevron / Jeffrey Jacobs

Aug 12 Alloy Ventures / Craig Taylor

Aug 12 Earth Partners LLC / Emily McGlynn



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Aug 13 Michigan Molecular Institute (MMI) / Pat Smith

Aug 13 Novozymes / Sarah Teter

Aug 14 Verdezyne / Bill Jankowski

Aug 14 Google / Corey Anderson

Aug 14 Hawaii Forest / Marius Ellis

Aug 14 Benson Hill Biosystems / Ben Gray

Aug 14 Innosense LLC / Anamika Ray

Aug 15 Genesys / Goutham Vemuri

Aug 15 BASF / Lahoucine Achnine

Aug 17 Bolt Threads, Inc. / Gustavo Pesce

Aug 17 DuPont / Luis Cascao-Pereira


Aug 18 POET – DSM jv / Dan Cummings

Aug 20 Cascade Clean Energy / Charles Zhou & others

Aug 20 Illium Tech / Aaron Socha (meeting)

Aug 21 Aemetis / Eric McAfee (call)

Aug 20 Agilent / Steffen Mueller



12 / 22

Aug 25 Zeachem / Nick Bilotti

Aug 27 Virdia / Manja Sachet

Aug 26 Cargill / Gary Folkert


Aug 29 Cooley LLC / Fred Dorey



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Milestones

Feedstocks Division

Area Initiatives Milestone/Deliverables Due % Complete

Plant Cell Wall Biosynthesis and Its Regulation

Novel acetyl and feruloyltransferase activities characterized (CWB) Sept. 2015 95

Genetic characterization of mutant candidates from rice stem saccharification screen (GG)

Sept. 2015 93

Correlation between cell wall modification and environmental interactions investigated (GG)

Sept. 2015 93

Characterization of novel nucleotide sugar transporters (SB) Sept. 2015 100

Characterization of novel GT activities (SB) Sept. 2015 95

Lignin and Hemicellulose Modification for Fuels Production

Evaluation of engineered plants with new lignin traits (including analysis with the Deconstruction and Technology Divisions) (CWE)

Sept. 2015 99

Transgenic plants with dominant repressors of hemicellulose biosynthesis characterized (CWB)

March 2016 80

Nucleotide sugar transporters used for cell wall engineering (CWB) Sept. 2015 95

Optimization of translation control technologies to improve tissue-specific expression (CWE)

Sept. 2015 85

Analysis of stacked traits in Arabidopsis (CWE) Sept. 2015 100

Heterologous screening of transferases to promote in planta design of polysaccharides (SB).

Nov. 2015 70



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Milestones

Feedstocks Division (continued)


Engineering of Modifications in Different Plant Species

Cell wall related genes in switchgrass functionally annotated, integrated into phylogenomics databases, and integrated with Kbase (GG)

Sept. 2015 93

Resequencing of Kitaake mutants (with JGI) and a database set up for hi throughput in silico screening (GG).

Sept. 2015 95

Translation of best technologies developed in Arabidopsis to other plant species (CWE)

Sept. 2015 95



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Milestones

Deconstruction Division


Biomass Pretreatment

Predict and develop task specific ILs for selective lignin or cellulose dissolution and low temperature pretreatment

May 2015 100

Complete characterization of lignin streams from various IL pretreatment process configurations

July 2015 100

Design, synthesis and assessment of lignin and hemicellulose derived IL mixtures for mixed and engineered feedstock pretreatment

Aug. 2015 100

Use multi-scale simulations strategies (from first principle quantum mechanics to force field based) to understand interactions between biomass derived ILs and lignocellulosic biomass

Sept. 2015 95

Enzyme Optimization

Develop an assay for screening lignin degrading enzymes on insoluble lignin and begin populating a lignin degrading enzyme database (LigDB).

Feb. 2015 100

Expand our database of ionic liquid tolerant enzymes, particularly to include enzymes from the GH3, GH5, GH6, GH9, GH10, AA9 and AA10 families.

May 2015 100

Improve the overall sugar yields of our existing thermo and ionic liquid tolerant multi-component enzyme mixture by including enzymes for hydrolysis of both cellulose and hemicellulose and by including LPMOs.

Dec. 2015 60

Demonstrate using directed evolution to engineer enhanced stability -glucosidase in up to 20% [C2mim][OAc].

Sept. 2015 95



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Milestones

Deconstruction Division (continued)


Microbial Communities

Complete characterization of active component of JTherm March 2015 100

Establish complementary thermophilic cellulase cocktail focusing on crystalline substrates

July 2015 100

Identify five bacterial strains capable of metabolizing mixtures of aromatics from pretreatment-derived lignin streams

Sept. 2015 100

Fungal Biotechnology

Design new expression systems based on recent “omics” discoveries in regard to promoters and assess heterologous GH expression

Feb. 2015 100

Initiate ChIP-Seq analysis of histone modifications in A. niger aimed at identifying epigenetic determinants of high productivity

April 2015 100

Optimize cultivation conditions for heterologous enzyme production, including temperature and pH, and initiate ‘omic analysis of optimal vs. baseline conditions

July 2015 100

Validate candidate genes for hyperproduction phenotypes through gene deletion and/or overexpression

Sept. 2015 92



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Milestones

Fuels Synthesis Division


Discovery of Novel Hydrocarbon Biochemistries

In vitro, anaerobic testing of toluene biosynthesis candidates Sept. 2015 92

In vitro, anaerobic testing of ladderane biosynthesis candidates Sept. 2015 92

Optimization of Hydrocarbon Biosynthetic Pathways

Improve methyl ketone production in E. coli with NIMS-aided HT screening and metabolic modeling

Sept. 2015 92

E. coli pathway engineering for isopentenol production both in aerobic and anaerobic conditions

Sept. 2015 95

Genes encoding isoprenoid pathway integrated into E. coli chromosome

Sept. 2015 92

Three enzymes in S. cerevisiae glycolytic pathway replaced and impact on fatty acid-derived fuel tested

Sept. 2015 100

Type 1 fatty acid synthases from several organisms expressed in E. coli and production of fatty acid-based fuels tested

March 2015 100

Develop analytical method for LC-MS of MEV and DXP intermediates April 2015 100

Host Engineering Improve overall xylose utilization capability in S. cerevisiae strains and examine performance in industrially relevant yeast strains.

Sept. 2015 100

E. coli membrane editing: With JGI, generate 10-20 single and double mutants and test improvement in expression of beneficial tolerance pump and corresponding tolerance to toxic fuels.

Sept. 2015 100



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Milestones

Fuels Synthesis Division (continued)


Synthetic Bio Informatics

Codon optimization tool (GeneDesign) integrated into the DIVA platform.

Sept. 2015 92

Clonal sequence validation (Sanger and/or MiSeq) pipeline implemented and integrated with DIVA/ICE

Sept. 2015 92

Quantitative Metabolic Modeling for Host and Pathway Engineering

Usage of EDD, MvT and 2S-13C MFA to improve production of fatty acids in S. cerevisiae.

Sept. 2015 100

Perform flux analysis of S. cerevisiae sip1 strain June 2015 100

Implement interactive metabolic engineering on MvT Sept. 2015 95



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Milestones

Technology Division


High Throughput Screening

Support the optimization of T and IL tolerant cellulose/hemicellulase cocktail using HT-NIMS

Sept. 2015 100

Apply HT NIMS screen for fatty acids based fuel molecules (e.g. FAMK) in collaboration with the Fuels Division.

Sept. 2015 100

Implement software to automate unit operations (e.g., droplet dispensing, routing, merger, and sorting) on an integrated droplet chip

Sept. 2015 100

Demonstrate microfluidic droplet platform for integration of DNA assembly, transformation of cells, and culturing

July 2015 100

Proteomics Develop rapid untargeted proteomics methods for relative quantification of >800 E. coli and S. cerevisiae proteins

Sept. 2015 100

Develop absolute quantification targeted proteomic assays for 150 S. cerevisiae proteins

Sept. 2015 100

Implement membrane proteomics assays to enable E. coli membrane editing with Fuels Synthesis Division

Sept. 2015 100



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Milestones

Technology Division (continued)


Physical Characterization

SEM and TEM analysis of genetically altered feedstock biomass (including lignin and protein mapping) with Feedstocks and Deconstruction Divisions

Sept. 2015 92

Electron tomographic analysis of plant cell walls of engineered feedstocks, and CAD model generation for mechanical properties simulation.

Sept. 2015 92

Structural Biology Test expression of multiple GT clones in insect cell platform to produce quantities of protein for crystallization trials

Sept. 2015 100

Characterize lignin degrading enzymes in collaboration with GLBRC Sept. 2015 100

Structural characterization of Fatty Acid Synthase and other large complexes by single particle cryo electron microscopy

Sept. 2015 70

Synthetic Bio Informatics

EDD further developed to support enzyme characterization data and workflows, with Deconstruction Division

Sept. 2015 92

EDD further developed to support genetic expression tool data and workflows, with Fuels Synthesis

Sept. 2015 92

BioCad tools enabled to query ICE and EDD for enzymes and expression tools satisfying specified performance characteristics

Sept. 2015 92



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Milestones

Operations Management Division


Intellectual Property Management

Generate >10 inventions Sept. 2015 100

Educate researchers about the tech transfer process Sept. 2015 98

Licensing Increase number of patent applications or inventions licensed or optioned and/or licensing agreements executed relative to the average of years 1 - 7.

Sept. 2015 95

Start-up Company Creation

Identify any IP or portfolios of IP that could lead to a start-up company and create an internal commercialization team to develop an initial go-to-market plan around that IP.

Sept. 2015 96

Maintain regular JBEI on-site office hours for the Berkeley Lab entrepreneur adviser.

Sept. 2015 95

Business Development Outreach

Promote each JBEI invention to at least 700 contacts in the biofuels industry.

Sept. 2015 97

Conduct meetings with at least fifteen companies each year, at least one-third of which are new to JBEI

Sept. 2015 93

Industry Advisory Committee

Hold an annual meeting of the IAC to inform JBEI research of industry concerns and technology bottlenecks.

Sept. 2015 100

Industry Partnership Program

Strengthen relationships with current partners and pursue collaborations with new industry partners in areas of complementary research.

Sept. 2015 97



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Milestones

Operations Management Division (continued)


Annual Scientific Advisory Meeting

Hold the annual meeting and report to DOE on the findings of the committee

Sept. 2015 100

Annual JBEI Conference

Annual JBEI Retreat Sept. 2015 100

Human Resources Staffing for year 8 complete Sept. 2015 92

Documents

This Monthly Progress Report provides a summary of JBEI’s ... · PDF fileThis Monthly Progress Report provides a ... Sr. Business Editor from Chemical & Engineering News gave a seminar