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The effect of low level laser on the guinea pig femur fracture healing. Hee-Gon Park * , Manghyang-Ro, Dongnam-Gu, Cheonan-Si, Chungcheongnam-Do Department of Orthopaedic Surgery, Dankook University College of Medicine, Korea Abstract Purpose: This study was conducted to evaluate the effectiveness of the low level laser on the fracture- healing of the guinea pig femurs. Materials and methods: This study was performed on 36 guinea pigs (17 in the experimental group and 19 in the control group. After anesthesia of the guinea pigs, the shaft of femur was fractured with Gigli saw under aseptic conditions, and then the intramedullary nailing of K-wire was performed. The application of low level laser on the fracture sites in the experimental group began 2 days after the operation under anesthesia, for 30 minutes every 2 days. The laser we used was a CW-type microchip laser with output wave length of 890 nm and intensity of 13 mWatts, measuring up to 4.68 J/cm 3 per each application. In order to compare the femur fracture healing process of both groups; gross findings, simple radiologic findings, and histologic findings of fracture site stability were evaluated. The simple radiologic and histologic findings were assessed by the scoring system invented by Zorlu and his colleagues. The statistical evaluation was done by using repetitive measured ANOVA test. Results: In both experimental and control groups, the gross findings progressed favorably. Although the improvement in radiologic findings were found in both groups with the passage of time, the experimental group showed a greater rate of callus formation with statistical significance (p<0.01) compared to the control group. The result of histological findings showed that an increase of osteoblastic proliferation was also greater in the experimental group (p<0.01). Conclusion: In this study, the low level laser was revealed to be effective on acceleration of femur fracture healing in guinea pigs. Keywords: Guinea Pig, Fracture healing, Low level laser. Accepted on February 16, 2016 Introduction The healing of fracture needs long duration of time and has possibilities of many complications such as non-union and delayed union. Now there are variable reports about the methods for promoting fracture healing. A decent amount of ultrasounds, electronic stimulations, fine motion exercise is known as a method for shortening the fracture healing processes [1-6]. Lately, there is a report of laser as having same effects with ultrasounds or electronic stimulations [7-10]. Although orthopaedic surgery department or dental surgery department had done experiments partially for using ultrasounds with laser simultaneously [7,9-11], there is much controversies in report that studied laser-only therapy so far. Laser is some kind of a light wave or an electromagnetic wave; it has some different characters from beams that we know normally. Laser is specific lights and kinds of electromagnetic waves that have relatively short wavelength, that composed of electrical wave and magnetic wave which propagated in light speeds. Living body cells that absorbed low level laser transfer light energy into chemical energy which can be used as healer of cell damage; it is called bio-stimulation effect. Photo- irradiation using low level laser can induce biological and physiological effect in variable enzymes, tissues and organs. In case of low level laser, effects for some soft tissues like promotion of collagen synthesis and wound healing procedure, it is already been reported [12-15]. It is also known as increasing manifestation of growth factor in cell. So, we conducted study on hypothesis that low level laser can stimulate fracture healing by biomechanical and biochemical character for guinea pig; increase of blood circulation, shortening of cell proliferation, and ATP synthesis-induced potential energy increase, light energy effect, etc. Material and Methods This experiment is for female guinea pig which is 9 weeks of birth and English Hartley species. Their body weights are from 552 gm to 635 gm, mean 610 ± 56 gm. We divided 42 guinea pigs into two groups; experimental groups that we broke their right femur, and control groups that we broke their left femur. We excluded 3 pigs which died at 3 weeks and 5 weeks, and 3 pigs which founds to be had infection at wound site. So the last experimental group consisted of 17, and control group consisted of 19 guinea pigs. ISSN 0970-938X www.biomedres.info Biomed Res- India 2016 Volume 27 Issue 3 604 Biomedical Research 2016; 27 (3): 604-610

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The effect of low level laser on the guinea pig …Translate this pagePDF-1.4 1 0 obj >endobj 2 0 obj >endobj 3 0 obj >/Parent 2 0 R /Resources >/ProcSet [/PDF /Text /ImageC ]/ExtGState